FastQCFastQC Report
Wed 25 May 2016
SRR2431376_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431376_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3588590
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA161580.4502604086841907No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37600.039.7495161
GTATCAA63250.034.9244771
TCAACGC65300.032.9643754
ATCAACG65900.032.7355653
CAACGCA69700.030.9508445
AACGCAG72850.029.8706056
TATCAAC79700.027.3033072
ACGCAGA84200.025.3417347
CGCAGAG83600.025.1862968
GTACATG125200.023.3870941
TACATGG122700.023.365652
ACATGGG124150.021.99493
GCAGAGT99250.020.5992479
CATGGGT39900.020.2604064
CATGGGG66200.018.3169984
ATAACGC3157.774361E-917.9045453
TCTGTCG8200.017.7680768
AGAGTAC88100.016.9912810-11
CAGAGTA98500.016.53330210-11
CGCCGTA1850.003373392615.2441294
Produced by FastQC (version 0.11.5)