##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2431376_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3588590 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.3547005927119 33.0 31.0 34.0 28.0 34.0 2 31.453592915323288 34.0 31.0 34.0 28.0 34.0 3 31.517148796602566 34.0 31.0 34.0 28.0 34.0 4 34.75803922989252 37.0 35.0 37.0 32.0 37.0 5 34.86569488294845 37.0 35.0 37.0 32.0 37.0 6 34.969088695002775 37.0 35.0 37.0 32.0 37.0 7 34.91341947673042 37.0 35.0 37.0 32.0 37.0 8 34.91872100184195 37.0 35.0 37.0 32.0 37.0 9 36.48153703822393 39.0 38.0 39.0 33.0 39.0 10-11 36.50108719580671 39.0 38.0 39.0 32.5 39.0 12-13 36.44703783380102 39.0 38.0 39.0 32.0 39.0 14-15 37.862816315042956 41.0 39.0 41.0 33.0 41.0 16-17 37.86605909284705 41.0 39.0 41.0 33.0 41.0 18-19 37.888153146500436 41.0 39.0 41.0 33.0 41.0 20-21 37.9333748073756 41.0 39.0 41.0 33.0 41.0 22-23 37.93556257471597 41.0 39.0 41.0 32.0 41.0 24-25 37.95559927436681 41.0 39.0 41.0 32.0 41.0 26-27 37.95466227682739 41.0 39.0 41.0 32.0 41.0 28-29 37.926904717451706 41.0 38.0 41.0 32.0 41.0 30-31 37.866339147130205 41.0 38.0 41.0 32.0 41.0 32-33 37.79885776865008 40.5 38.0 41.0 32.0 41.0 34-35 37.743105509406206 40.0 38.0 41.0 31.5 41.0 36-37 37.66133829721423 40.0 38.0 41.0 31.0 41.0 38-39 37.570265340983504 40.0 38.0 41.0 31.0 41.0 40-41 37.49629729225127 40.0 38.0 41.0 31.0 41.0 42-43 37.39123653022496 40.0 38.0 41.0 30.5 41.0 44-45 37.27887485056805 40.0 38.0 41.0 30.0 41.0 46-47 37.16764258385605 40.0 38.0 41.0 30.0 41.0 48-49 37.053773766298185 40.0 37.5 41.0 30.0 41.0 50-51 36.94010600263613 40.0 37.0 41.0 30.0 41.0 52-53 36.79284273210369 40.0 37.0 41.0 30.0 41.0 54-55 36.65297526326496 40.0 36.5 41.0 30.0 41.0 56-57 36.48530509197205 40.0 36.0 41.0 30.0 41.0 58-59 36.29019782142847 40.0 36.0 41.0 30.0 41.0 60-61 36.13996304955428 39.0 35.0 41.0 29.0 41.0 62-63 36.003225779484424 39.0 35.0 41.0 29.0 41.0 64-65 35.771994153692674 39.0 35.0 41.0 29.0 41.0 66-67 35.49169520619519 38.5 35.0 40.5 29.0 41.0 68-69 35.195637423054734 38.0 35.0 40.0 28.0 41.0 70-71 34.9567816328976 37.0 35.0 40.0 28.0 41.0 72-73 34.71510565430991 37.0 34.5 40.0 28.0 41.0 74-75 34.368510752133844 36.5 34.0 39.0 28.0 41.0 76-77 34.00149682744476 36.0 34.0 39.0 28.0 41.0 78-79 33.6401115758557 35.5 34.0 38.5 27.0 40.0 80-81 33.26826190788025 35.0 34.0 37.0 27.0 39.0 82-83 32.89870199716323 35.0 34.0 37.0 26.0 39.0 84-85 32.5740953967993 35.0 33.5 36.5 26.0 38.5 86-87 32.276653225918814 35.0 33.0 36.0 26.0 37.0 88-89 32.00157791778943 35.0 33.0 36.0 25.5 37.0 90-91 31.770529650921393 35.0 33.0 35.5 25.0 37.0 92-93 31.546449998467367 35.0 33.0 35.0 25.0 36.0 94-95 31.32790343839781 35.0 33.0 35.0 24.5 36.0 96-97 31.136037273692452 35.0 33.0 35.0 24.0 36.0 98-99 31.00921643319521 35.0 33.0 35.0 23.0 36.0 100 30.960643595395407 35.0 33.0 35.0 22.0 36.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3640496801244666 1101 2 0.4076710179353995 1101 3 0.38740408572067864 1101 4 0.38695423072019963 1101 5 0.3812400029296015 1101 6 0.4033869090642881 1101 7 0.4210263565865233 1101 8 0.4474766190675723 1101 9 0.537815504789954 1101 10-11 0.4765916717367844 1101 12-13 0.5373896859956133 1101 14-15 0.6277559400382131 1101 16-17 0.6102589330553343 1101 18-19 0.5481516129127257 1101 20-21 0.5131595706749366 1101 22-23 0.5732768080012249 1101 24-25 0.5477724789964924 1101 26-27 0.558463281656941 1101 28-29 0.5659489960033923 1101 30-31 0.6190870545130522 1101 32-33 0.5736261944894139 1101 34-35 0.6251267909346581 1101 36-37 0.5729603242162966 1101 38-39 0.5285787053371749 1101 40-41 0.5368558567441895 1101 42-43 0.6007550767941296 1101 44-45 0.6142006359857746 1101 46-47 0.606671774256192 1101 48-49 0.616607983826583 1101 50-51 0.5719153587575576 1101 52-53 0.6313422414876371 1101 54-55 0.6561768107635189 1101 56-57 0.6413090598957254 1101 58-59 0.6539830590390139 1101 60-61 0.6169573437285152 1101 62-63 0.5865331558990263 1101 64-65 0.645359100112529 1101 66-67 0.6108130278026067 1101 68-69 0.6521509043806404 1101 70-71 0.617085116603171 1101 72-73 0.6240896731426915 1101 74-75 0.6191286461496901 1101 76-77 0.625372048816935 1101 78-79 0.608728249412728 1101 80-81 0.6611052330808747 1101 82-83 0.6336948970501695 1101 84-85 0.652317050621285 1101 86-87 0.6370417814289269 1101 88-89 0.6788400844449995 1101 90-91 0.7199018031257864 1101 92-93 0.7557069456603003 1101 94-95 0.764401799212056 1101 96-97 0.8019549929109466 1101 98-99 0.788211326403065 1101 100 0.7998559770226699 1102 1 0.09852226579424794 1102 2 0.14306696809116204 1102 3 0.16681031625027387 1102 4 0.07842962012829702 1102 5 0.10276710573333503 1102 6 0.11466229847238907 1102 7 0.17226592044945477 1102 8 0.1534115100333011 1102 9 0.2133133241045968 1102 10-11 0.17006207672120865 1102 12-13 0.18708812836321442 1102 14-15 0.25746824533416657 1102 16-17 0.24199288944161879 1102 18-19 0.21712466587222679 1102 20-21 0.19107779497399946 1102 22-23 0.10403054631898101 1102 24-25 0.22472170018029658 1102 26-27 0.1839246523734559 1102 28-29 0.22526021095665527 1102 30-31 0.2430574594974786 1102 32-33 0.2363361633367731 1102 34-35 0.2282228968536586 1102 36-37 0.2546955267085025 1102 38-39 0.2394452162405969 1102 40-41 0.21559090347316356 1102 42-43 0.27981816090627376 1102 44-45 0.23892898489543768 1102 46-47 0.20534560603189078 1102 48-49 0.17704785921598898 1102 50-51 0.19033031202858552 1102 52-53 0.18998149382408513 1102 54-55 0.23582836839591437 1102 56-57 0.14645516581472862 1102 58-59 0.13602433629758082 1102 60-61 0.18226435307431643 1102 62-63 0.16453190979310506 1102 64-65 0.1328709381187565 1102 66-67 0.1770170776468376 1102 68-69 0.15791320967659317 1102 70-71 0.08975411971842817 1102 72-73 0.13180291301807756 1102 74-75 0.16837506359517107 1102 76-77 0.14307921392596512 1102 78-79 0.142472018883133 1102 80-81 0.1891588156353876 1102 82-83 0.1656740247760311 1102 84-85 0.11322592912596008 1102 86-87 0.16391234217659445 1102 88-89 0.14887248319890034 1102 90-91 0.10084183427843385 1102 92-93 0.14264573070702724 1102 94-95 0.09589136307498691 1102 96-97 0.07180717359630862 1102 98-99 0.12120836690150583 1102 100 0.13437778387624988 1103 1 -0.001922665749081176 1103 2 0.00527889660390457 1103 3 0.06459786157256175 1103 4 0.054996027056617436 1103 5 -0.004831023266973489 1103 6 0.08172506151652925 1103 7 0.06933535796529355 1103 8 0.049455386055953454 1103 9 0.09103051365345749 1103 10-11 0.04207532349963827 1103 12-13 0.029369871144325543 1103 14-15 0.12373127980579568 1103 16-17 0.08282131241321622 1103 18-19 0.09097103383059135 1103 20-21 0.08063613163613326 1103 22-23 0.07630190156040584 1103 24-25 0.054987054533228275 1103 26-27 0.06532012625020656 1103 28-29 0.03278852722261405 1103 30-31 0.07652678269899127 1103 32-33 0.02605111077856037 1103 34-35 0.10963756501383415 1103 36-37 0.10182707623520315 1103 38-39 0.0717336787466607 1103 40-41 0.025312602854093313 1103 42-43 0.056656888887886225 1103 44-45 0.03673778390777471 1103 46-47 -0.08135578138691102 1103 48-49 0.023026025977458175 1103 50-51 -0.01618698514632655 1103 52-53 -0.012106468380395086 1103 54-55 -0.02874385104464494 1103 56-57 -0.08593383945428457 1103 58-59 -0.07836911744315955 1103 60-61 -0.08303180340561767 1103 62-63 -0.13141076767788462 1103 64-65 -0.09485153608994779 1103 66-67 -0.10967042699353158 1103 68-69 -0.10455996818188851 1103 70-71 -0.11835983120065663 1103 72-73 -0.14531028746396402 1103 74-75 -0.09841228353319309 1103 76-77 -0.09231644832705399 1103 78-79 -0.17793838442615595 1103 80-81 -0.16318176711610022 1103 82-83 -0.13470650141348273 1103 84-85 -0.149220077604042 1103 86-87 -0.11876865780684653 1103 88-89 -0.09570594667573573 1103 90-91 -0.13250985730359943 1103 92-93 -0.1227378642668242 1103 94-95 -0.10005057423682828 1103 96-97 -0.07779178668330289 1103 98-99 -0.12572217763475635 1103 100 -0.15474752898481725 1104 1 -0.022252965378179113 1104 2 0.034341312719980976 1104 3 0.03052366244025606 1104 4 -0.047405708811169234 1104 5 -0.004426966080224304 1104 6 0.00677859462740571 1104 7 0.014673522119402094 1104 8 0.010472688606498082 1104 9 -0.010636951748210777 1104 10-11 0.03168577604517253 1104 12-13 0.046344032178531336 1104 14-15 8.233625627838137E-4 1104 16-17 -0.012952858327892613 1104 18-19 -0.0576338746156182 1104 20-21 0.0034252547263307065 1104 22-23 0.0389960823776363 1104 24-25 0.030577921173360778 1104 26-27 0.040683840991789566 1104 28-29 0.12726093855818732 1104 30-31 0.12579793886679624 1104 32-33 0.08530321640930083 1104 34-35 0.04385604263533338 1104 36-37 0.030060475387870156 1104 38-39 0.037355878428634526 1104 40-41 0.05303884994147268 1104 42-43 0.04460352728543171 1104 44-45 0.01890501996159344 1104 46-47 0.012556808269785336 1104 48-49 0.021139805444121862 1104 50-51 -0.029936039579553153 1104 52-53 -0.030944908928091763 1104 54-55 -0.05240051349952779 1104 56-57 0.0017944718382807423 1104 58-59 -0.028082700598169197 1104 60-61 3.0193934453848215E-4 1104 62-63 -0.014210381515944448 1104 64-65 0.06871087637858864 1104 66-67 0.10362822054636922 1104 68-69 0.06904515986060744 1104 70-71 0.0702627432093621 1104 72-73 0.0178716837247066 1104 74-75 0.0651757360967764 1104 76-77 0.07467628162115858 1104 78-79 0.061756594083931304 1104 80-81 0.021524809800446576 1104 82-83 -0.006937075738939313 1104 84-85 -0.009263448244922756 1104 86-87 0.007623489667771821 1104 88-89 0.04409344801266002 1104 90-91 0.007898023836293788 1104 92-93 -0.0016783151106771754 1104 94-95 0.043906485531028494 1104 96-97 -0.03769398668088897 1104 98-99 -0.146021175486581 1104 100 -0.14336246591005875 1105 1 0.1752181840614746 1105 2 0.22664897069130419 1105 3 0.18175684162619277 1105 4 0.27694005749184925 1105 5 0.2143297667091275 1105 6 0.2141569878044507 1105 7 0.22641848257077868 1105 8 0.22799787891009515 1105 9 0.2829934575458637 1105 10-11 0.27687119929584014 1105 12-13 0.3408810245782874 1105 14-15 0.3072779872823119 1105 16-17 0.4011384606143835 1105 18-19 0.3666492507080008 1105 20-21 0.2935020903599721 1105 22-23 0.38139413083586504 1105 24-25 0.35929531364216416 1105 26-27 0.3101735178774163 1105 28-29 0.3371245865545731 1105 30-31 0.3665988655366803 1105 32-33 0.3667860370091063 1105 34-35 0.37722285392678145 1105 36-37 0.33600363130290134 1105 38-39 0.37389602817182066 1105 40-41 0.3427999512323794 1105 42-43 0.4021409035657797 1105 44-45 0.379740793466091 1105 46-47 0.23737334905933238 1105 48-49 0.27118987359036595 1105 50-51 0.29702874458433115 1105 52-53 0.3069005092041692 1105 54-55 0.38710436981863694 1105 56-57 0.28920512288785005 1105 58-59 0.3412704606138206 1105 60-61 0.37971403664190007 1105 62-63 0.36392449448170083 1105 64-65 0.43563941507316173 1105 66-67 0.40534452386559394 1105 68-69 0.4564926366641089 1105 70-71 0.4642977150283585 1105 72-73 0.43681723219781077 1105 74-75 0.41440187449614996 1105 76-77 0.45555315117913864 1105 78-79 0.46743691083005245 1105 80-81 0.45878161103362913 1105 82-83 0.5157114324832435 1105 84-85 0.49500208999136674 1105 86-87 0.4646827263108193 1105 88-89 0.45935032066547876 1105 90-91 0.5284136141494074 1105 92-93 0.45868489841620885 1105 94-95 0.4743057362199288 1105 96-97 0.4838691661392964 1105 98-99 0.460649121678653 1105 100 0.38472211875495077 1106 1 -0.02873269873015971 1106 2 -0.02415629483992987 1106 3 -0.02320031956566382 1106 4 -0.06644576927980239 1106 5 -0.06260404993383872 1106 6 0.001020389240501629 1106 7 0.032941775088730196 1106 8 0.03380349131426641 1106 9 0.047430482763523685 1106 10-11 0.00925907261784431 1106 12-13 0.02943109568724367 1106 14-15 0.03624404576508766 1106 16-17 0.04822897710819518 1106 18-19 0.03442562172330099 1106 20-21 0.03110450459563907 1106 22-23 -0.007082663364421649 1106 24-25 -0.01633148576121357 1106 26-27 0.04516900852918582 1106 28-29 0.03647454666418071 1106 30-31 0.03224344217824893 1106 32-33 0.029733242947571625 1106 34-35 0.03931709930470362 1106 36-37 0.010455918929949348 1106 38-39 0.03372319872924123 1106 40-41 0.08165717833009722 1106 42-43 0.04744450410690604 1106 44-45 0.0507032791575881 1106 46-47 0.04138146588613978 1106 48-49 0.10050401906887885 1106 50-51 0.019952363162843767 1106 52-53 -0.007310181419853734 1106 54-55 -0.02960380597657064 1106 56-57 -0.02874468459766888 1106 58-59 -0.028801081930147632 1106 60-61 -0.06603781045650692 1106 62-63 -0.01864878242696477 1106 64-65 -0.0062682126628033075 1106 66-67 0.0415200762607526 1106 68-69 0.010802446230862017 1106 70-71 0.04063577739611901 1106 72-73 0.031386589442249146 1106 74-75 0.06040485760343017 1106 76-77 0.014977775689182238 1106 78-79 0.05629873399422536 1106 80-81 0.08053210532756339 1106 82-83 0.08356758427483868 1106 84-85 0.009303834762256713 1106 86-87 0.03409200169324578 1106 88-89 0.04086563510580277 1106 90-91 -0.023331677050421717 1106 92-93 0.03416817914047243 1106 94-95 0.04065862740588955 1106 96-97 -0.008593024710197739 1106 98-99 0.03331969644711563 1106 100 0.05649914882883422 1107 1 -0.1025285640168434 1107 2 -0.08245895758051702 1107 3 -0.06158591078159503 1107 4 -0.05542093542180737 1107 5 -0.052231210781485515 1107 6 0.04123496782609237 1107 7 0.08738578295938026 1107 8 0.042480256703107955 1107 9 0.004350726895587798 1107 10-11 -0.003592483562272264 1107 12-13 -0.035073482944575574 1107 14-15 -0.005190684655730138 1107 16-17 -8.860104750141318E-5 1107 18-19 -0.009794731963104653 1107 20-21 0.026401788233521017 1107 22-23 -0.010895000323372983 1107 24-25 -0.00410132303218802 1107 26-27 0.030791858082267254 1107 28-29 0.023223602509155228 1107 30-31 0.012018324187771157 1107 32-33 0.019949069620587068 1107 34-35 0.04619023969296876 1107 36-37 0.06909026475494784 1107 38-39 0.04023753709652311 1107 40-41 0.021884992805745185 1107 42-43 0.043726220550681205 1107 44-45 0.04045936281333695 1107 46-47 0.10200045561330029 1107 48-49 0.10347220208471697 1107 50-51 0.07222483759631615 1107 52-53 0.017218526902112785 1107 54-55 0.019364148993666674 1107 56-57 0.03465029809089515 1107 58-59 0.010616847076789782 1107 60-61 -0.01975070104238341 1107 62-63 0.053141375556243986 1107 64-65 0.08213776398101658 1107 66-67 0.07036829188095339 1107 68-69 0.10016639930893945 1107 70-71 0.0887122589606335 1107 72-73 0.07741908902237071 1107 74-75 0.07137240270792233 1107 76-77 0.12269576340245436 1107 78-79 0.05901313923433804 1107 80-81 0.03321026980700026 1107 82-83 0.10447443535027645 1107 84-85 -0.037948077045726336 1107 86-87 -0.039832370372401726 1107 88-89 0.012672403437662183 1107 90-91 -0.03286070124637064 1107 92-93 0.008386868710317685 1107 94-95 0.0722105085509881 1107 96-97 0.029840687209652828 1107 98-99 0.019936824829088096 1107 100 0.06946982801952117 1108 1 -0.15561154223318496 1108 2 -0.1834178206507069 1108 3 -0.16242436630787793 1108 4 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2214 86-87 -0.3108774227524904 2214 88-89 -0.3427297351469676 2214 90-91 -0.2576706129929107 2214 92-93 -0.25958944617919144 2214 94-95 -0.27720064556959656 2214 96-97 -0.3853596589184214 2214 98-99 -0.3033855712533189 2214 100 -0.22826170970806814 2215 1 -0.3124552807110419 2215 2 -0.363762924623348 2215 3 -0.3087611884307542 2215 4 -0.30782305387510434 2215 5 -0.2536892398902424 2215 6 -0.32756296038740373 2215 7 -0.3554705102542073 2215 8 -0.32512834176793604 2215 9 -0.35014036988367536 2215 10-11 -0.4014644640334524 2215 12-13 -0.4441413845004689 2215 14-15 -0.49620698424376997 2215 16-17 -0.5061299703389253 2215 18-19 -0.5034559588888428 2215 20-21 -0.5115427791945208 2215 22-23 -0.4151406854713642 2215 24-25 -0.43317438784423956 2215 26-27 -0.38486230581041525 2215 28-29 -0.3813722833699771 2215 30-31 -0.3753848776018245 2215 32-33 -0.4482727611241586 2215 34-35 -0.4959017991436738 2215 36-37 -0.505610824608766 2215 38-39 -0.47959048526335124 2215 40-41 -0.526017250305415 2215 42-43 -0.3896391790805467 2215 44-45 -0.3417702517426875 2215 46-47 -0.3461607322712297 2215 48-49 -0.3308068986799384 2215 50-51 -0.34784286578552326 2215 52-53 -0.38374627026171027 2215 54-55 -0.37111117879262423 2215 56-57 -0.31502266334187823 2215 58-59 -0.32291446054323103 2215 60-61 -0.3319678520939391 2215 62-63 -0.39500457256050225 2215 64-65 -0.3112012132033968 2215 66-67 -0.3256177815968755 2215 68-69 -0.3973285995358111 2215 70-71 -0.4855676249111909 2215 72-73 -0.4793291258129244 2215 74-75 -0.46684054445083234 2215 76-77 -0.37410379974704 2215 78-79 -0.3880759281329631 2215 80-81 -0.37577244315933456 2215 82-83 -0.3054319959002214 2215 84-85 -0.29175213997923066 2215 86-87 -0.3430878530358257 2215 88-89 -0.3890144847455943 2215 90-91 -0.36100811854522874 2215 92-93 -0.25047699689844904 2215 94-95 -0.22247822689760355 2215 96-97 -0.26274827079662444 2215 98-99 -0.2566361931791832 2215 100 -0.26306961044469546 2216 1 -0.1621687125847302 2216 2 -0.23562376251199169 2216 3 -0.3195182009404647 2216 4 -0.2721127121132696 2216 5 -0.25130067644074217 2216 6 -0.2502545159564207 2216 7 -0.23599369312349694 2216 8 -0.33402777198462275 2216 9 -0.2961806841081369 2216 10-11 -0.28711545336677347 2216 12-13 -0.30923052941449214 2216 14-15 -0.35869078738515014 2216 16-17 -0.38381960878393784 2216 18-19 -0.4849948659605161 2216 20-21 -0.37064863147916327 2216 22-23 -0.37390264518850813 2216 24-25 -0.4620664605229763 2216 26-27 -0.531569692659545 2216 28-29 -0.4115892807157522 2216 30-31 -0.44060101508097915 2216 32-33 -0.4923569869521671 2216 34-35 -0.46999400442574313 2216 36-37 -0.3960193775235581 2216 38-39 -0.39007928969182615 2216 40-41 -0.4482511026592917 2216 42-43 -0.4528252877460517 2216 44-45 -0.4939351552321227 2216 46-47 -0.39820552483246985 2216 48-49 -0.4577744519646316 2216 50-51 -0.44246707703366184 2216 52-53 -0.5649859275264504 2216 54-55 -0.5954886407692115 2216 56-57 -0.4285585457380776 2216 58-59 -0.4580567089808696 2216 60-61 -0.41752973166501306 2216 62-63 -0.43508929646220196 2216 64-65 -0.5258384143862287 2216 66-67 -0.585624006165915 2216 68-69 -0.6574432132664185 2216 70-71 -0.6676820747804229 2216 72-73 -0.5431245770644324 2216 74-75 -0.5298459188875881 2216 76-77 -0.5061188376785068 2216 78-79 -0.39573861057070303 2216 80-81 -0.4126512706473804 2216 82-83 -0.3818518303731864 2216 84-85 -0.35196132551789816 2216 86-87 -0.37461820200106644 2216 88-89 -0.47866463800735914 2216 90-91 -0.4069863493182879 2216 92-93 -0.4891408588136059 2216 94-95 -0.5455134451955637 2216 96-97 -0.6665706077518543 2216 98-99 -0.6779994441023227 2216 100 -0.6317115457610925 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 52698.0 3 10780.0 4 7630.0 5 7099.0 6 5026.0 7 4541.0 8 5128.0 9 5391.0 10 5693.0 11 6169.0 12 7253.0 13 7767.0 14 8368.0 15 8453.0 16 8422.0 17 8327.0 18 8233.0 19 8105.0 20 8247.0 21 8434.0 22 10373.0 23 15661.0 24 25259.0 25 32962.0 26 32933.0 27 25518.0 28 25887.0 29 31599.0 30 38075.0 31 45575.0 32 59330.0 33 79717.0 34 113512.0 35 179601.0 36 312261.0 37 641961.0 38 1119174.0 39 604114.0 40 3314.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.74917592821456 22.622968453007775 14.357143195517564 22.2707124232601 2 14.713243920314106 22.947229970545536 40.47620374576087 21.86332236337949 3 16.602760415650717 29.06534321279388 31.420613667206336 22.911282704349063 4 11.513686433947596 19.93401308034632 40.89394441828127 27.658356067424812 5 11.704624936256302 39.93507199206373 36.076314095508266 12.2839889761717 6 26.90700247172288 41.44076085593506 19.33611808537615 12.316118586965912 7 23.494157872590627 35.07305097545275 25.022780534973343 16.41001061698327 8 22.763731716356563 37.47276785589884 22.52054428062275 17.24295614712185 9 23.869876469588334 18.34193931321215 22.834845998010362 34.95333821918915 10-11 21.470828375490093 29.24767109087413 30.712912313749968 18.568588219885804 12-13 22.439913726561127 27.547017073558138 29.886515316600672 20.126553883280064 14-15 20.498134364750502 29.1081176729579 27.29227914027515 23.101468822016447 16-17 19.758735325016232 32.056629484003466 27.746357204361598 20.4382779866187 18-19 19.687370248481994 31.030181770556126 29.6239748759262 19.658473105035682 20-21 21.37455100749877 30.2409860140055 28.735018489155905 19.649444489339825 22-23 20.814024449714232 30.337960034442496 28.803778085543346 20.044237430299923 24-25 20.6527772746399 30.351252163105848 28.72794050030792 20.268030061946334 26-27 20.450330073928757 30.18312206186831 28.826238160391686 20.540309703811246 28-29 20.443906938379698 30.101028537670782 29.042186485499876 20.41287803844964 30-31 20.852674727399897 30.515690006381334 28.570803574663028 20.060831691555737 32-33 20.19050657779239 30.309299752827712 29.120671350028843 20.379522319351054 34-35 20.488227966973103 29.926280238199404 29.03976213498895 20.545729659838543 36-37 20.89483613341173 30.299198292365524 28.50699021063983 20.29897536358291 38-39 20.591374328078714 30.254668268038422 28.852279028810756 20.301678375072104 40-41 20.463733666983412 30.026319529397338 29.02616347924951 20.48378332436974 42-43 20.32507196419764 30.030610908462656 29.059881457619845 20.584435669719863 44-45 20.59179231954612 30.034498229109484 28.885760145349565 20.487949305994835 46-47 20.588629517442783 30.26364115153863 28.738264889552724 20.409464441465868 48-49 20.425027657102092 29.95729520508055 29.03170883271703 20.585968305100334 50-51 20.570906679224986 29.879144733725504 28.809198041570642 20.740750545478864 52-53 20.438068990885 29.957587799107728 28.920648499828623 20.68369471017865 54-55 20.630944186992664 30.212896987396164 28.68726993052982 20.468888895081356 56-57 20.52647418624028 30.026793253060397 28.71914874644359 20.727583814255738 58-59 20.421460796580273 29.640192944861354 28.94019656745407 20.998149691104306 60-61 20.806988260012986 30.076771099512623 28.571653490646742 20.54458714982765 62-63 20.474448181597786 30.04769282643044 28.813196826608777 20.664662165362998 64-65 20.53610192303941 29.692177150357104 28.941492341003013 20.830228585600473 66-67 20.44939655965156 29.851208970654213 28.683981730986265 21.015412738707962 68-69 20.86958944878072 29.92726948467225 28.611390546147653 20.591750520399376 70-71 20.864058028362116 29.74593085306485 28.701189046394266 20.688822072178766 72-73 20.551915933556074 29.901674473818407 28.989895752928028 20.556513839697484 74-75 20.59214064576895 29.773434691619826 28.853505137115132 20.780919525496085 76-77 20.626388079997994 29.973750135847226 28.627984807403468 20.771876976751315 78-79 20.764548193022886 30.023992710228807 28.695114237068044 20.516344859680263 80-81 20.73049582147863 29.833513998534244 28.708824357198786 20.727165822788336 82-83 20.458247534647715 29.912504498640065 28.977442219905925 20.651805746806296 84-85 20.574673406603207 29.74007679960764 28.759516351962905 20.925733441826246 86-87 20.72300753120073 29.95789650269831 28.625724459375935 20.693371506725022 88-89 20.487951689717345 29.78443459190128 28.797147530841837 20.930466187539537 90-91 20.508189601540593 29.692598558694783 28.734659083704855 21.06455275605977 92-93 20.58249351017771 29.914754158662515 28.704862625882903 20.797889705276866 94-95 20.492126944228865 29.588965321213152 28.825945623595338 21.09296211096264 96-97 20.43550423616284 29.846061328569235 28.760637780558877 20.95779665470905 98-99 20.349830130240218 29.74119018766836 28.81642654440893 21.092553137682497 100 20.518906241683435 29.800547364044466 28.617926687996086 21.062619706276013 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 13448.0 1 8741.5 2 3287.5 3 2098.0 4 1374.0 5 998.5 6 918.0 7 1067.5 8 1306.0 9 1331.0 10 1057.5 11 807.0 12 791.5 13 859.0 14 898.5 15 1017.0 16 1319.5 17 1790.5 18 2427.0 19 3397.0 20 5115.0 21 7194.5 22 9950.0 23 14211.5 24 19813.0 25 26582.0 26 35440.0 27 46783.5 28 60071.5 29 74344.0 30 86794.0 31 98064.0 32 108353.5 33 117369.5 34 125383.0 35 131733.0 36 135910.0 37 138374.5 38 140258.5 39 140738.5 40 140266.0 41 137318.5 42 132320.0 43 128539.0 44 123834.0 45 117583.0 46 112471.0 47 106613.0 48 99436.5 49 94891.5 50 91325.5 51 85741.5 52 78974.5 53 72826.5 54 67777.5 55 63329.5 56 57881.0 57 51388.5 58 45898.0 59 40981.0 60 35532.0 61 29625.0 62 24732.5 63 19980.0 64 15323.5 65 11850.0 66 9152.5 67 6941.5 68 5249.0 69 3959.5 70 3085.5 71 2395.0 72 1731.5 73 1215.0 74 894.0 75 656.5 76 454.5 77 345.0 78 267.5 79 190.0 80 132.0 81 112.5 82 91.5 83 76.5 84 68.0 85 59.5 86 51.5 87 33.5 88 27.0 89 29.5 90 23.0 91 17.0 92 16.0 93 14.5 94 12.0 95 15.0 96 14.5 97 10.5 98 14.0 99 18.0 100 47.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.9506268478706123E-4 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.5326353804697668E-4 84-85 8.359829348016909E-4 86-87 0.0014490370869895976 88-89 0.0033439317392067636 90-91 0.0029677394185460026 92-93 0.002410417462011542 94-95 0.0023686183152714577 96-97 0.002312886119618012 98-99 0.0029398733207192797 100 0.0033160656413800406 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3588590.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.37112866307513 #Duplication Level Percentage of deduplicated Percentage of total 1 80.01784394988394 56.309459919391514 2 13.561435471639575 19.086670408614786 3 3.5219451161811617 7.435297587452208 4 1.2074991086817273 3.3989230055036153 5 0.5766680365320126 2.029039029733858 6 0.2938409522224088 1.2406751673194185 7 0.16760048624507645 0.825596476708236 8 0.11994649772970796 0.6752616339538022 9 0.0880702032243203 0.5577839642233635 >10 0.4077382491227899 5.076339399591409 >50 0.024531429538954305 1.1723184810348994 >100 0.012405382250316429 1.5399377484268228 >500 4.3552368735659525E-4 0.20250163134212512 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 3.959306056736093E-5 0.4501955467038833 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 16158 0.4502604086841907 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 2.786609782672303E-5 0.0 0.0 0.0 10-11 2.786609782672303E-5 2.786609782672303E-5 0.0 0.0 0.0 12-13 2.786609782672303E-5 2.786609782672303E-5 0.0 0.0 0.0 14-15 2.786609782672303E-5 2.786609782672303E-5 0.0 0.0 0.0 16-17 2.786609782672303E-5 2.786609782672303E-5 0.0 0.0 0.0 18-19 2.786609782672303E-5 2.786609782672303E-5 0.0 1.3933048913361515E-5 0.0 20-21 2.786609782672303E-5 2.786609782672303E-5 0.0 2.786609782672303E-5 0.0 22-23 2.786609782672303E-5 2.786609782672303E-5 0.0 2.0899573370042275E-4 0.0 24-25 2.786609782672303E-5 2.786609782672303E-5 0.0 4.737236630542915E-4 0.0 26-27 4.1799146740084545E-5 2.786609782672303E-5 0.0 7.245185434947988E-4 0.0 28-29 6.966524456680758E-5 2.786609782672303E-5 0.0 0.0018252294076503586 0.0 30-31 8.359829348016909E-5 2.786609782672303E-5 0.0 0.004333178212055431 0.0 32-33 8.359829348016909E-5 2.786609782672303E-5 0.0 0.01001786216870693 0.0 34-35 8.359829348016909E-5 2.786609782672303E-5 0.0 0.019506268478706124 0.0 36-37 8.359829348016909E-5 2.786609782672303E-5 0.0 0.03473509094101026 0.0 38-39 8.359829348016909E-5 2.786609782672303E-5 0.0 0.05503554320777798 0.0 40-41 8.359829348016909E-5 2.786609782672303E-5 0.0 0.07632524194739437 0.0 42-43 8.359829348016909E-5 5.573219565344606E-5 0.0 0.09633310018698152 0.0 44-45 8.359829348016909E-5 5.573219565344606E-5 0.0 0.12293129056258865 0.0 46-47 8.359829348016909E-5 5.573219565344606E-5 0.0 0.1540437887861249 0.0 48-49 8.359829348016909E-5 5.573219565344606E-5 0.0 0.18902967460757567 0.0 50-51 8.359829348016909E-5 6.966524456680758E-5 0.0 0.22691363460300562 0.0 52-53 1.6719658696033818E-4 1.1146439130689212E-4 0.0 0.2637665489788469 0.0 54-55 1.6719658696033818E-4 1.1146439130689212E-4 0.0 0.3049805076645702 0.0 56-57 1.6719658696033818E-4 1.1146439130689212E-4 0.0 0.35647705644835437 0.0 58-59 1.6719658696033818E-4 1.6719658696033818E-4 0.0 0.4145360712703318 0.0 60-61 1.6719658696033818E-4 2.0899573370042272E-4 0.0 0.4771233269891517 0.0 62-63 1.6719658696033818E-4 2.2292878261378425E-4 0.0 0.5383312108655489 0.0 64-65 1.6719658696033818E-4 2.5079488044050727E-4 0.0 0.6093061620302125 0.0 66-67 1.6719658696033818E-4 2.647279293538688E-4 0.0 0.6925282631897207 0.0 68-69 1.6719658696033818E-4 3.3439317392067636E-4 0.0 0.7889310286212691 0.0 70-71 1.6719658696033818E-4 3.3439317392067636E-4 0.0 0.8938608199877947 0.0 72-73 1.811296358736997E-4 3.3439317392067636E-4 0.0 1.0079306914414854 0.0 74-75 2.0899573370042272E-4 3.3439317392067636E-4 0.0 1.1287998907648964 0.0 76-77 2.2292878261378425E-4 3.901253695741224E-4 0.0 1.2623899637462068 0.0 78-79 2.2292878261378425E-4 3.901253695741224E-4 0.0 1.4170746727823462 0.0 80-81 2.2292878261378425E-4 3.901253695741224E-4 0.0 1.589106027715621 0.0 82-83 2.2292878261378425E-4 3.901253695741224E-4 0.0 1.7723116878774114 0.0 84-85 2.2292878261378425E-4 3.901253695741224E-4 0.0 1.9612298980936802 0.0 86-87 2.2292878261378425E-4 3.901253695741224E-4 0.0 2.166003917973354 0.0 88 2.2292878261378425E-4 3.901253695741224E-4 0.0 2.3353183283685235 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3760 0.0 39.749516 1 GTATCAA 6325 0.0 34.924477 1 TCAACGC 6530 0.0 32.964375 4 ATCAACG 6590 0.0 32.735565 3 CAACGCA 6970 0.0 30.950844 5 AACGCAG 7285 0.0 29.870605 6 TATCAAC 7970 0.0 27.303307 2 ACGCAGA 8420 0.0 25.341734 7 CGCAGAG 8360 0.0 25.186296 8 GTACATG 12520 0.0 23.387094 1 TACATGG 12270 0.0 23.36565 2 ACATGGG 12415 0.0 21.9949 3 GCAGAGT 9925 0.0 20.599247 9 CATGGGT 3990 0.0 20.260406 4 CATGGGG 6620 0.0 18.316998 4 ATAACGC 315 7.774361E-9 17.904545 3 TCTGTCG 820 0.0 17.768076 8 AGAGTAC 8810 0.0 16.99128 10-11 CAGAGTA 9850 0.0 16.533302 10-11 CGCCGTA 185 0.0033733926 15.24412 94 >>END_MODULE