FastQCFastQC Report
Wed 25 May 2016
SRR2431376_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431376_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3588590
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163020.4542731267712388No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125340.3492736701601465No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83740.23335070320097867No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64450.059.950011
GTATCAA129650.044.158581
ATCAACG161900.035.1837123
TCAACGC162200.035.06024
CAACGCA167150.034.0214465
TATCAAC171700.033.2850462
AACGCAG172400.033.0399326
ACGCAGA196750.028.831447
CGCAGAG196650.028.7505058
GCAGAGT214000.025.9583749
GTGGTAT24900.025.1068731
GTACATG114550.023.4304891
TACATGG110000.022.9439132
TGGTATC25650.022.9038522
AGAGTAC203100.022.28384610-11
ACATGGG112150.022.0011773
GAGTACT150550.021.96125212-13
CAGAGTA215000.021.19254710-11
AGTACTT158900.020.80721512-13
GTACTTT170550.019.2205714-15
Produced by FastQC (version 0.11.5)