##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431376_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3588590
Sequences flagged as poor quality	0
Sequence length	100
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.786405802836214	34.0	31.0	34.0	31.0	34.0
2	32.930956448075705	34.0	31.0	34.0	31.0	34.0
3	32.99922866641216	34.0	31.0	34.0	31.0	34.0
4	36.37565116104096	37.0	37.0	37.0	35.0	37.0
5	36.33373581267294	37.0	37.0	37.0	35.0	37.0
6	36.36458999216963	37.0	37.0	37.0	35.0	37.0
7	36.23466152444275	37.0	37.0	37.0	35.0	37.0
8	36.290008889285204	37.0	37.0	37.0	35.0	37.0
9	38.11698689457419	39.0	39.0	39.0	37.0	39.0
10-11	38.10837877829454	39.0	39.0	39.0	37.0	39.0
12-13	38.09290598814576	39.0	39.0	39.0	37.0	39.0
14-15	39.60256563162691	41.0	40.0	41.0	37.0	41.0
16-17	39.610064537882565	41.0	40.0	41.0	37.0	41.0
18-19	39.67910168060436	41.0	40.0	41.0	37.0	41.0
20-21	39.685363471447005	41.0	40.0	41.0	37.0	41.0
22-23	39.61497356900621	41.0	40.0	41.0	37.0	41.0
24-25	39.591765010770246	41.0	40.0	41.0	37.0	41.0
26-27	39.51397624136499	41.0	40.0	41.0	36.5	41.0
28-29	39.43355830562979	41.0	39.5	41.0	36.0	41.0
30-31	39.290213705104236	41.0	39.0	41.0	36.0	41.0
32-33	39.11027924616632	41.0	39.0	41.0	35.0	41.0
34-35	39.018446381447866	41.0	39.0	41.0	35.0	41.0
36-37	38.91670628296907	41.0	39.0	41.0	35.0	41.0
38-39	38.80350611242856	41.0	39.0	41.0	35.0	41.0
40-41	38.71878648717184	41.0	39.0	41.0	35.0	41.0
42-43	38.61058507658997	41.0	39.0	41.0	35.0	41.0
44-45	38.44492837019554	41.0	38.5	41.0	35.0	41.0
46-47	38.1962469939447	41.0	38.0	41.0	34.5	41.0
48-49	37.97776940246726	41.0	38.0	41.0	34.0	41.0
50-51	37.85358371951101	41.0	38.0	41.0	33.5	41.0
52-53	37.76076996814905	40.0	38.0	41.0	33.0	41.0
54-55	37.63773236842326	40.0	38.0	41.0	33.0	41.0
56-57	37.50279469095104	40.0	37.5	41.0	33.0	41.0
58-59	37.348122242997945	40.0	37.0	41.0	33.0	41.0
60-61	37.18015905968639	40.0	37.0	41.0	33.0	41.0
62-63	36.97980822551476	40.0	36.0	41.0	32.5	41.0
64-65	36.735284471059664	39.0	36.0	41.0	32.0	41.0
66-67	36.473143351567046	39.0	35.0	41.0	31.5	41.0
68-69	36.21991032689719	39.0	35.0	41.0	31.0	41.0
70-71	35.9695268893911	38.0	35.0	40.5	31.0	41.0
72-73	35.70675780738396	37.0	35.0	40.0	31.0	41.0
74-75	35.39759083651239	37.0	35.0	39.5	31.0	41.0
76-77	35.0589203559058	36.5	35.0	39.0	31.0	41.0
78-79	34.71383008368189	36.0	35.0	39.0	31.0	40.5
80-81	34.37013172304442	36.0	35.0	37.5	31.0	39.0
82-83	34.01270958788828	35.0	35.0	37.0	30.5	39.0
84-85	33.71360144234922	35.0	35.0	37.0	30.0	39.0
86-87	33.45572021323138	35.0	34.5	36.0	30.0	37.0
88-89	33.20214248493141	35.0	34.0	36.0	30.0	37.0
90-91	32.980427131547486	35.0	34.0	36.0	30.0	37.0
92-93	32.81143081265901	35.0	34.0	35.0	30.0	36.0
94-95	32.66615425557113	35.0	34.0	35.0	29.5	36.0
96-97	32.522036231500394	35.0	34.0	35.0	29.0	36.0
98-99	32.396506566645954	35.0	34.0	35.0	29.0	36.0
100	32.29492001036619	35.0	34.0	35.0	29.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.22144218271397875
1101	2	0.205035003173748
1101	3	0.20771333915603662
1101	4	0.15697215599674763
1101	5	0.16336229675882663
1101	6	0.17812225492607325
1101	7	0.21462704962154788
1101	8	0.21000908169576604
1101	9	0.31134207317282403
1101	10-11	0.29013867909645796
1101	12-13	0.2423105965541268
1101	14-15	0.34736566066994357
1101	16-17	0.33072896242843797
1101	18-19	0.32401825151159613
1101	20-21	0.3360220992294174
1101	22-23	0.3654221662965398
1101	24-25	0.32995976981563047
1101	26-27	0.3473999988236258
1101	28-29	0.3099101381795606
1101	30-31	0.3346276597343518
1101	32-33	0.37681402961285926
1101	34-35	0.40680326959432733
1101	36-37	0.4215970941417595
1101	38-39	0.44916338630897457
1101	40-41	0.4230406387744807
1101	42-43	0.4441246924256532
1101	44-45	0.36521187250988874
1101	46-47	0.3554965101172556
1101	48-49	0.33429927044667807
1101	50-51	0.3819925168562861
1101	52-53	0.3137798375693208
1101	54-55	0.35195274348508576
1101	56-57	0.39588381787349647
1101	58-59	0.4115350034126024
1101	60-61	0.40662883175413356
1101	62-63	0.3498134822607142
1101	64-65	0.46241590061939064
1101	66-67	0.4501257457981822
1101	68-69	0.4118550342483047
1101	70-71	0.39715512277954446
1101	72-73	0.34807521410223785
1101	74-75	0.333408874210825
1101	76-77	0.37986770323590946
1101	78-79	0.373555775378243
1101	80-81	0.35797318756340246
1101	82-83	0.3957455813715569
1101	84-85	0.34291576142616975
1101	86-87	0.3471472830160707
1101	88-89	0.3231345785706523
1101	90-91	0.35541962070642796
1101	92-93	0.37917939206461426
1101	94-95	0.35441136214647173
1101	96-97	0.3973514304687811
1101	98-99	0.40187460471093317
1101	100	0.4775448136498923
1102	1	0.14212099891335583
1102	2	0.17668391283107354
1102	3	0.1718398189068182
1102	4	0.13542137718054903
1102	5	0.14794765501427776
1102	6	0.15528549480146125
1102	7	0.18307004027575147
1102	8	0.18420627795744338
1102	9	0.23783085821955297
1102	10-11	0.22720861679116666
1102	12-13	0.23444533175661064
1102	14-15	0.32699167313100475
1102	16-17	0.331853105730616
1102	18-19	0.2974870988636198
1102	20-21	0.3188330026562127
1102	22-23	0.30190035944296056
1102	24-25	0.33208438040753663
1102	26-27	0.3431643290416915
1102	28-29	0.3800740011079071
1102	30-31	0.3554798404197044
1102	32-33	0.3412627835069415
1102	34-35	0.35611417302111903
1102	36-37	0.30980176703895523
1102	38-39	0.26311665733701517
1102	40-41	0.2674613552853913
1102	42-43	0.2745390226437223
1102	44-45	0.3575584457186167
1102	46-47	0.2984412141671058
1102	48-49	0.29269459754948457
1102	50-51	0.2631027972301183
1102	52-53	0.27815086560669755
1102	54-55	0.2769025877218496
1102	56-57	0.30916434746850996
1102	58-59	0.3254226981166468
1102	60-61	0.28544409655163605
1102	62-63	0.30482018008002854
1102	64-65	0.2889735330181509
1102	66-67	0.331177770720295
1102	68-69	0.3255798006034425
1102	70-71	0.2715163377328125
1102	72-73	0.2670594820150143
1102	74-75	0.20122164679649757
1102	76-77	0.18729184653808773
1102	78-79	0.17736029251219776
1102	80-81	0.18710324986869864
1102	82-83	0.19706598635598738
1102	84-85	0.21768616641058713
1102	86-87	0.22471441697245353
1102	88-89	0.22853286517501203
1102	90-91	0.27011339017682445
1102	92-93	0.252961946581749
1102	94-95	0.2552791191558086
1102	96-97	0.29204052704198347
1102	98-99	0.3040750720006571
1102	100	0.3217728510330673
1103	1	0.13431293315715465
1103	2	0.1579395924981526
1103	3	0.17243171615032082
1103	4	0.12531594169696092
1103	5	0.10837663593550673
1103	6	0.13959020844192338
1103	7	0.13018493813839171
1103	8	0.16384749020472356
1103	9	0.24085749566642534
1103	10-11	0.22448492688058508
1103	12-13	0.23997959399356006
1103	14-15	0.2994834270674289
1103	16-17	0.31008738275539827
1103	18-19	0.2936168333512654
1103	20-21	0.302920385619025
1103	22-23	0.3570661489634688
1103	24-25	0.3213319384191422
1103	26-27	0.3149264317564544
1103	28-29	0.298930465144295
1103	30-31	0.2921153690114835
1103	32-33	0.3140011676865271
1103	34-35	0.266757593013665
1103	36-37	0.26705147665111895
1103	38-39	0.22270051453430284
1103	40-41	0.2524012060386198
1103	42-43	0.2573004852166747
1103	44-45	0.20552731470015573
1103	46-47	0.08657500135223728
1103	48-49	0.12492440339920563
1103	50-51	0.09938503212120509
1103	52-53	0.11144178360044066
1103	54-55	0.10756812658534187
1103	56-57	0.14521887794833788
1103	58-59	0.12246968925068558
1103	60-61	0.01849192019218293
1103	62-63	0.08891645645807955
1103	64-65	0.1213114097783432
1103	66-67	0.08024095162839018
1103	68-69	0.02188710042911879
1103	70-71	0.04694523505057191
1103	72-73	0.07312640575085538
1103	74-75	0.07288441094512166
1103	76-77	0.08239386041535113
1103	78-79	0.06403588174026709
1103	80-81	0.07958713280665819
1103	82-83	0.11888103537982886
1103	84-85	0.09319557233813214
1103	86-87	0.08239321565345392
1103	88-89	0.07851013500161486
1103	90-91	0.11636760179762717
1103	92-93	0.14987180884617857
1103	94-95	0.04087435210116297
1103	96-97	0.041875736161109955
1103	98-99	0.07946692300914293
1103	100	0.12285925130992581
1104	1	0.06457536487572924
1104	2	0.09976168495757776
1104	3	0.09472376999504206
1104	4	0.028626426593874044
1104	5	0.0625476331155923
1104	6	0.08427282181949636
1104	7	0.05260331568352683
1104	8	0.09166622455290963
1104	9	0.08209808224312098
1104	10-11	0.1269331613337954
1104	12-13	0.17196630328125906
1104	14-15	0.17346399778256938
1104	16-17	0.17412450286683168
1104	18-19	0.18701749565369852
1104	20-21	0.15915316498905696
1104	22-23	0.14667575662156196
1104	24-25	0.17111887790331082
1104	26-27	0.21753098899747414
1104	28-29	0.1877134639543101
1104	30-31	0.20804406185075663
1104	32-33	0.22093413543296947
1104	34-35	0.20588255908790387
1104	36-37	0.25187532543427693
1104	38-39	0.21095628124473365
1104	40-41	0.16575144754243354
1104	42-43	0.15988924269020544
1104	44-45	0.11777687628917732
1104	46-47	0.19046589787236456
1104	48-49	0.167467222071771
1104	50-51	0.12306069523874896
1104	52-53	0.094154213986549
1104	54-55	0.0857968100005877
1104	56-57	0.07860936587047718
1104	58-59	0.0697848900339153
1104	60-61	0.08259338201867905
1104	62-63	0.0490834747021367
1104	64-65	0.13610259752037734
1104	66-67	0.039286819116391314
1104	68-69	0.040692675971662595
1104	70-71	0.07910568967296427
1104	72-73	0.09172230404932691
1104	74-75	0.10016450535216848
1104	76-77	0.11880171684135377
1104	78-79	0.11537928255889085
1104	80-81	0.06728233344398404
1104	82-83	0.09064830245999644
1104	84-85	0.10321387178142061
1104	86-87	0.10965778944085969
1104	88-89	0.05340540245575909
1104	90-91	0.056437836881784165
1104	92-93	0.07776185616136644
1104	94-95	0.0792960182311262
1104	96-97	0.05914666856401851
1104	98-99	0.04394096903443767
1104	100	0.03836764055843389
1105	1	-0.01367750232539322
1105	2	0.038787759079262685
1105	3	0.04200625254186008
1105	4	-0.07439477313711507
1105	5	-0.10114321505219692
1105	6	-0.10500530412905107
1105	7	-0.4383461787249061
1105	8	-0.4098523356270718
1105	9	-0.43200595832323785
1105	10-11	-0.3777873051555076
1105	12-13	-0.25382719872146
1105	14-15	-0.23521780389698677
1105	16-17	-0.15169072261093675
1105	18-19	-0.18009985872462408
1105	20-21	-0.12129994801467348
1105	22-23	-0.10110145575070106
1105	24-25	-0.10678038766467779
1105	26-27	-0.11254724527086779
1105	28-29	-0.14374183032438026
1105	30-31	-0.1673006867223421
1105	32-33	-0.11795683652886879
1105	34-35	-0.15931405324031545
1105	36-37	-0.13149818144878367
1105	38-39	-0.14169881841542775
1105	40-41	-0.12417510925700981
1105	42-43	-0.10572963828300885
1105	44-45	-0.08365426922239294
1105	46-47	-0.19146805681187828
1105	48-49	-0.11876135947458266
1105	50-51	-0.03624134141143287
1105	52-53	-0.050218587627533395
1105	54-55	-0.05598583919207556
1105	56-57	-0.042292560079260966
1105	58-59	-0.02478074461890145
1105	60-61	-0.05044675879705096
1105	62-63	-0.07659754136133046
1105	64-65	-0.013273075758561959
1105	66-67	0.012959604065898134
1105	68-69	0.013058183854603556
1105	70-71	0.0035086660866383568
1105	72-73	0.04586970229121334
1105	74-75	-0.04562813366319318
1105	76-77	0.03317715205480454
1105	78-79	0.013358924984537168
1105	80-81	0.004107045831119649
1105	82-83	-6.032375260787148E-4
1105	84-85	-0.019697624400606628
1105	86-87	-0.0262101972988944
1105	88-89	-0.0428362875710846
1105	90-91	-0.014813450159714137
1105	92-93	-0.05869147407711495
1105	94-95	-0.1340721024204612
1105	96-97	-0.17176353016114376
1105	98-99	-0.11974980473788577
1105	100	-0.1024047926493239
1106	1	0.08807125760384338
1106	2	0.05325791066273666
1106	3	0.09738382373753751
1106	4	0.02415894013771691
1106	5	0.07696493993063314
1106	6	0.07157071307585028
1106	7	0.06994599235282806
1106	8	0.10497648257682357
1106	9	0.13874207317282838
1106	10-11	0.1378959478189259
1106	12-13	0.13381059655412741
1106	14-15	0.12669121133073702
1106	16-17	0.1591329271861497
1106	18-19	0.1746438021723904
1106	20-21	0.09308201112369119
1106	22-23	0.11666225440227151
1106	24-25	0.10234523237069482
1106	26-27	0.14073347899983446
1106	28-29	0.11610485183594221
1106	30-31	0.13035497250967865
1106	32-33	0.06749288423841193
1106	34-35	0.06725745461634602
1106	36-37	0.044402821014010385
1106	38-39	0.004352813621757434
1106	40-41	0.05063359031633752
1106	42-43	0.1086114765666224
1106	44-45	0.14488808396363595
1106	46-47	0.12576302994104793
1106	48-49	0.1228182131779505
1106	50-51	0.11561278117346774
1106	52-53	0.08454944109355012
1106	54-55	0.086288866833101
1106	56-57	-0.003195036752060787
1106	58-59	0.05728566420555126
1106	60-61	0.007879492547075984
1106	62-63	0.038693658472169545
1106	64-65	0.02641369797622417
1106	66-67	0.014222221569106352
1106	68-69	0.016316708257114954
1106	70-71	0.02422296418924219
1106	72-73	-0.007784257263395489
1106	74-75	-0.0025721830579001903
1106	76-77	-0.01792833200638455
1106	78-79	-0.015552154137175478
1106	80-81	-0.01280214283306691
1106	82-83	-0.02864340541258059
1106	84-85	-7.820359306549562E-4
1106	86-87	-0.0042906024464883785
1106	88-89	-0.011329298081328432
1106	90-91	-0.0183781766504012
1106	92-93	-0.01335188546842403
1106	94-95	0.013277441441623239
1106	96-97	0.04287962430137071
1106	98-99	0.008774164182298705
1106	100	-0.029277212781828155
1107	1	-0.06478321078024862
1107	2	-0.030630544220443312
1107	3	-0.010489389070613697
1107	4	-0.0777274592533388
1107	5	-0.10459412156926362
1107	6	-0.0668033834090096
1107	7	-0.03458743096607009
1107	8	-0.062471086195110104
1107	9	-0.05626317341688747
1107	10-11	-0.025924524821000716
1107	12-13	0.006612275462835271
1107	14-15	0.022380456052943032
1107	16-17	7.537613091628259E-4
1107	18-19	-0.001748100359691307
1107	20-21	0.015338013884893087
1107	22-23	0.005252701448696939
1107	24-25	0.018772466562751333
1107	26-27	-0.00394875948347817
1107	28-29	-0.04710791008906767
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2216	38-39	-0.7206228605841503
2216	40-41	-0.7550475136695951
2216	42-43	-0.771526923150148
2216	44-45	-0.769322924507513
2216	46-47	-0.7749256108438018
2216	48-49	-0.7390016409037798
2216	50-51	-0.6704152710476095
2216	52-53	-0.774602349671774
2216	54-55	-0.8296741827783762
2216	56-57	-0.7640141937255152
2216	58-59	-0.8616701332899694
2216	60-61	-0.7537109362657546
2216	62-63	-0.8492521349720903
2216	64-65	-0.8808007522389332
2216	66-67	-0.8168370545332237
2216	68-69	-0.8658046177815208
2216	70-71	-0.786092267444154
2216	72-73	-0.8444965340336381
2216	74-75	-0.712610761248996
2216	76-77	-0.716832959564421
2216	78-79	-0.6984885493939217
2216	80-81	-0.717093920970143
2216	82-83	-0.7400773680899135
2216	84-85	-0.6971135674884934
2216	86-87	-0.7051910765531133
2216	88-89	-0.6593718837325824
2216	90-91	-0.6418182417625076
2216	92-93	-0.8618882135691841
2216	94-95	-0.7734539236861764
2216	96-97	-0.8738325794732269
2216	98-99	-0.9148493389510364
2216	100	-0.8980745732598017
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	50.0
3	324.0
4	174.0
5	249.0
6	336.0
7	559.0
8	1025.0
9	1568.0
10	2210.0
11	3307.0
12	5157.0
13	7721.0
14	8582.0
15	10830.0
16	13591.0
17	20567.0
18	25267.0
19	13608.0
20	8096.0
21	7104.0
22	7146.0
23	7353.0
24	8350.0
25	10385.0
26	12865.0
27	16452.0
28	20541.0
29	26111.0
30	32645.0
31	41570.0
32	52512.0
33	68396.0
34	94940.0
35	151651.0
36	252838.0
37	578547.0
38	1236737.0
39	829150.0
40	10076.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.19503607469827	22.72924760795586	15.307103826964072	23.7686124903818
2	13.021468883731183	22.962813518070753	40.83415515123075	23.18156244696732
3	14.647182270652385	29.115826004763445	32.73463800872824	23.502353715855932
4	10.183555752109307	19.523723136763792	41.372372475303756	28.920348635823146
5	11.058465859850248	38.89480269409434	36.77906921660039	13.267662229455023
6	26.68513260082651	41.31736977475833	19.137432807871615	12.860064816543545
7	23.06145310553727	34.581186482713264	25.526320922702233	16.83103948904723
8	23.35817131519622	35.83070230926353	22.70356881114867	18.107557564391584
9	24.420705625329166	16.1577945655536	23.48735854472091	35.934141264396324
10-11	21.747705923496415	27.822320187037246	31.23072014356614	19.1992537459002
12-13	23.25119336563943	26.16575033648313	30.78164404403958	19.801412253837857
14-15	20.868823131090483	27.4612591574964	28.948152338383597	22.721765373029516
16-17	19.18731312298145	30.776906807409038	29.287881870038092	20.74789819957142
18-19	18.998213783129305	28.93681083656812	32.6550121356856	19.409963244616968
20-21	20.500349719527726	28.21322580735052	32.15195940466869	19.134465068453068
22-23	19.992570894178993	28.35612502744812	32.1964237744762	19.454880303896687
24-25	19.850428511516377	28.342410727347968	32.09269113034804	19.714469630787622
26-27	19.69330106047872	28.22545093195949	32.203667312421	19.877580695140793
28-29	19.65324482974345	28.155357797219217	32.373768828957616	19.81762854407972
30-31	20.12043682068975	28.53547861855603	31.921442276838047	19.422642283916172
32-33	19.47327250620816	28.316409919571868	32.437707281280545	19.77261029293943
34-35	19.769687798706386	28.130052753463254	32.11179665532453	19.988462792505832
36-37	20.21552007615574	28.493680812476523	31.52391459677698	19.766884514590757
38-39	20.011297252906033	28.452858770683882	31.707423443276728	19.828420533133357
40-41	19.919636701487605	28.338833380614602	31.744352860591217	19.997177057306573
42-43	19.818663119106375	28.397276407771955	31.65551064288988	20.128549830231794
44-45	20.14162161514461	28.4509191875943	31.294999563197273	20.112459634063814
46-47	20.289252899196214	28.75139875793222	30.804554555077583	20.154793787793984
48-49	20.228803152961287	28.63664882773427	30.714773681868984	20.419774337435463
50-51	20.366186032636783	28.668433299889077	30.381126572131954	20.58425409534218
52-53	20.270889161326476	28.802263396245237	30.398001777578624	20.52884566484966
54-55	20.459936927680843	29.073415967713125	30.128610795526583	20.338036309079442
56-57	20.319734557256155	28.78727058587814	30.258636412465634	20.634358444400075
58-59	20.174178015198255	28.398428900648447	30.54776693780531	20.879626146347988
60-61	20.52636345523375	28.849936674204457	30.09135912901603	20.532340741545763
62-63	20.25656316269064	28.772052533167624	30.391226080438276	20.58015822370346
64-65	20.242184256212052	28.52351480665108	30.54004497588189	20.694255961254978
66-67	20.268555740649607	28.66611621466625	30.250258738448725	20.815069306235415
68-69	20.626395317682043	28.677609110829692	30.112110288111026	20.58388528337724
70-71	20.669157298697634	28.56269037304722	30.100012163811	20.668140164444136
72-73	20.372929408447	28.732721616412917	30.306112041839295	20.588236933300784
74-75	20.43067954293071	28.60588572153105	30.2006958310053	20.762738904532934
76-77	20.48283218747492	28.767162796607902	30.010538999313376	20.7394660166038
78-79	20.579463951815743	28.842657691473843	30.018199510778427	20.559678845931987
80-81	20.530613877068536	28.66728873597087	30.035525386850797	20.766572000109793
82-83	20.247454051711767	28.728008148149183	30.314180259687422	20.710357540451632
84-85	20.360853219147952	28.56165741571701	30.057219481793556	21.020269883341477
86-87	20.461165248070724	28.770195816817417	29.940866639998763	20.8277722951131
88-89	20.187310994715375	28.569057919077594	30.116393258063763	21.12723782814327
90-91	20.216474156485216	28.52922444305947	30.025192896832014	21.229108503623298
92-93	20.182414255838925	28.704963005929667	30.081311574634444	21.03131116359696
94-95	20.10815505922097	28.40389778438459	30.13647707159839	21.351470084796052
96-97	20.047961550503214	28.618061671737728	30.037498960220006	21.29647781753906
98-99	19.917879439749903	28.501650199964917	30.09033428828081	21.49013607200437
100	20.12114902355403	28.558999886581283	29.84963242025229	21.470218669612397
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2142.0
1	1337.0
2	1612.0
3	2343.0
4	1273.0
5	686.5
6	1661.0
7	4024.0
8	6969.5
9	7694.0
10	4985.0
11	2601.0
12	2257.5
13	2421.5
14	2761.0
15	3091.5
16	3547.0
17	4209.5
18	4937.0
19	5912.0
20	7497.0
21	9618.5
22	12158.0
23	16178.0
24	21894.5
25	28467.5
26	37192.5
27	47983.5
28	60722.0
29	74775.5
30	87215.5
31	98526.0
32	108855.0
33	117601.5
34	124790.5
35	131141.0
36	135387.0
37	137747.0
38	139731.5
39	140110.0
40	139552.0
41	137103.5
42	132274.5
43	128843.5
44	124795.0
45	117779.0
46	111955.5
47	106571.0
48	99406.0
49	94130.5
50	90408.5
51	84529.5
52	77496.5
53	71296.0
54	65985.5
55	60994.0
56	55073.5
57	48921.5
58	43776.0
59	38827.5
60	33534.0
61	27940.0
62	23055.0
63	18387.0
64	13941.5
65	10469.5
66	7890.5
67	5941.0
68	4291.5
69	2991.0
70	2182.5
71	1592.5
72	1129.0
73	805.5
74	551.0
75	401.0
76	268.0
77	177.0
78	132.0
79	68.5
80	33.5
81	19.0
82	11.0
83	5.5
84	5.5
85	6.0
86	3.5
87	2.5
88	2.0
89	2.5
90	3.5
91	2.0
92	1.5
93	1.5
94	3.0
95	10.0
96	12.0
97	7.0
98	3.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009502339358912554
2	2.786609782672303E-5
3	2.5079488044050727E-4
4	0.00248008270657835
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	5.573219565344606E-5
24-25	1.3933048913361516E-4
26-27	6.130541521879067E-4
28-29	9.75313423935306E-4
30-31	0.0019366937989572506
32-33	0.004709370532716192
34-35	0.005573219565344606
36-37	0.004291379065315347
38-39	0.002981672467459364
40-41	0.0037897893044343323
42-43	5.712550054478221E-4
44-45	3.761923206607609E-4
46-47	0.00385945454900114
48-49	0.0025218818533184343
50-51	8.777820815417755E-4
52-53	1.3933048913361515E-5
54-55	3.0652707609395336E-4
56-57	5.433889076210992E-4
58-59	5.573219565344606E-4
60-61	1.3933048913361516E-4
62-63	0.0
64-65	0.0
66-67	6.687863478413527E-4
68-69	7.105854945814372E-4
70-71	0.002131756483744312
72-73	5.573219565344606E-5
74-75	0.0020899573370042274
76-77	3.9012536957412245E-4
78-79	8.91715130455137E-4
80-81	8.220498858883294E-4
82-83	0.0012539744022025364
84-85	0.0011007108641555598
86-87	1.811296358736997E-4
88-89	0.0018391624565637199
90-91	0.002187488679397758
92-93	0.003051337712026172
94-95	0.0010728447663288369
96-97	0.0025218818533184343
98-99	0.002382551364184819
100	0.0032603334457265943
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3588590.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.5427016829762
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.55303484806207	53.056841990350904
2	14.451949545872415	19.52247433828568
3	3.8046700963093794	7.709330919514942
4	1.338249805005776	3.615560294272248
5	0.5883623358576401	1.9869790866165822
6	0.341408533441857	1.383579283577145
7	0.21057267215037168	0.9955853024347794
8	0.12905052418550503	0.6973136845674617
9	0.09044819734990131	0.549820405023061
>10	0.4445325317356438	5.357764872254866
>50	0.03103774942532828	1.4289467785472993
>100	0.01556727779807824	1.9084794699599321
>500	7.025928807655195E-4	0.34081079658990415
>1k	2.893029475230453E-4	0.40780611694317614
>5k	4.1328992503291814E-5	0.2337578494956631
>10k+	8.265798500658363E-5	0.8049488115663889
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16302	0.4542731267712388	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12534	0.3492736701601465	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8374	0.23335070320097867	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	2.786609782672303E-5	0.0
6	0.0	0.0	0.0	2.786609782672303E-5	0.0
7	0.0	0.0	0.0	2.786609782672303E-5	0.0
8	0.0	0.0	0.0	2.786609782672303E-5	0.0
9	1.1146439130689212E-4	0.0	0.0	2.786609782672303E-5	0.0
10-11	1.1146439130689212E-4	0.0	0.0	2.786609782672303E-5	0.0
12-13	1.1146439130689212E-4	0.0	0.0	2.786609782672303E-5	0.0
14-15	1.1146439130689212E-4	0.0	0.0	8.359829348016909E-5	0.0
16-17	1.1146439130689212E-4	0.0	0.0	8.359829348016909E-5	0.0
18-19	1.5326353804697666E-4	0.0	0.0	8.359829348016909E-5	0.0
20-21	1.950626847870612E-4	0.0	0.0	8.359829348016909E-5	0.0
22-23	1.950626847870612E-4	0.0	0.0	2.786609782672303E-4	0.0
24-25	1.950626847870612E-4	0.0	0.0	5.155228097943761E-4	0.0
26-27	2.0899573370042272E-4	0.0	0.0	7.523846413215218E-4	0.0
28-29	2.2292878261378425E-4	0.0	0.0	0.00183916245656372	0.0
30-31	2.2292878261378425E-4	0.0	0.0	0.004374977358795515	0.0
32-33	2.2292878261378425E-4	0.0	0.0	0.010003929119793569	0.0
34-35	2.2292878261378425E-4	0.0	0.0	0.019631665918926375	0.0
36-37	2.2292878261378425E-4	0.0	0.0	0.034930153625797315	0.0
38-39	2.2292878261378425E-4	4.1799146740084545E-5	0.0	0.05520273979473832	0.0
40-41	2.2292878261378425E-4	8.359829348016909E-5	0.0	0.07657603682783488	0.0
42-43	2.2292878261378425E-4	8.359829348016909E-5	0.0	0.09665356031198884	0.0
44-45	2.2292878261378425E-4	8.359829348016909E-5	0.0	0.12301488885606882	0.0
46-47	2.2292878261378425E-4	8.359829348016909E-5	0.0	0.15436424891113223	0.0
48-49	2.2292878261378425E-4	8.359829348016909E-5	0.0	0.18943373302606314	0.0
50-51	2.2292878261378425E-4	8.359829348016909E-5	0.0	0.2276799522932405	0.0
52-53	2.2292878261378425E-4	8.359829348016909E-5	0.0	0.2650483894788761	0.0
54-55	2.2292878261378425E-4	8.359829348016909E-5	0.0	0.3068057370722206	0.0
56-57	2.2292878261378425E-4	8.359829348016909E-5	0.0	0.35883174171471244	0.0
58-59	2.2292878261378425E-4	8.359829348016909E-5	0.0	0.41737841324865754	0.0
60-61	2.2292878261378425E-4	8.359829348016909E-5	0.0	0.48088525019575934	0.0
62-63	2.786609782672303E-4	8.359829348016909E-5	0.0	0.5424832594417306	0.0
64-65	2.786609782672303E-4	8.359829348016909E-5	0.0	0.6137508046335748	0.0
66-67	2.786609782672303E-4	8.359829348016909E-5	0.0	0.6975720268963577	0.0
68-69	2.786609782672303E-4	8.359829348016909E-5	0.0	0.7948665074583611	0.0
70-71	2.786609782672303E-4	1.1146439130689212E-4	0.0	0.9010781393249159	0.0
72-73	2.9259402718059184E-4	1.2539744022025363E-4	0.0	1.0169871732351705	0.0
74-75	3.065270760939533E-4	1.3933048913361516E-4	0.0	1.139765200259712	0.0
76-77	3.065270760939533E-4	1.3933048913361516E-4	0.0	1.275347699235633	0.0
78-79	3.065270760939533E-4	1.5326353804697666E-4	0.0	1.4322059639022569	0.0
80-81	3.065270760939533E-4	1.950626847870612E-4	0.0	1.6068567320312435	0.0
82-83	3.065270760939533E-4	1.950626847870612E-4	0.0	1.7923892113615654	0.0
84-85	3.065270760939533E-4	1.950626847870612E-4	0.0	1.9839547008713727	0.0
86-87	3.065270760939533E-4	1.950626847870612E-4	0.0	2.190971941626098	0.0
88	3.065270760939533E-4	1.950626847870612E-4	0.0	2.36307296180394	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6445	0.0	59.95001	1
GTATCAA	12965	0.0	44.15858	1
ATCAACG	16190	0.0	35.183712	3
TCAACGC	16220	0.0	35.0602	4
CAACGCA	16715	0.0	34.021446	5
TATCAAC	17170	0.0	33.285046	2
AACGCAG	17240	0.0	33.039932	6
ACGCAGA	19675	0.0	28.83144	7
CGCAGAG	19665	0.0	28.750505	8
GCAGAGT	21400	0.0	25.958374	9
GTGGTAT	2490	0.0	25.106873	1
GTACATG	11455	0.0	23.430489	1
TACATGG	11000	0.0	22.943913	2
TGGTATC	2565	0.0	22.903852	2
AGAGTAC	20310	0.0	22.283846	10-11
ACATGGG	11215	0.0	22.001177	3
GAGTACT	15055	0.0	21.961252	12-13
CAGAGTA	21500	0.0	21.192547	10-11
AGTACTT	15890	0.0	20.807215	12-13
GTACTTT	17055	0.0	19.22057	14-15
>>END_MODULE