FastQCFastQC Report
Wed 25 May 2016
SRR2431375_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431375_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3303143
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA698282.1139865879255No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA94400.28578841424667356No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50730.1535809984611626No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43380.13132946408920232No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41650.12609202810777492No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA39600.1198858178407656No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39020.11812991444814833No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG35030.10605051007479846No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA33680.10196349355749963No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA33320.10087362248621995No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51800.047.907021
GTATCAA92700.038.329841
TCAACGC112550.030.3169924
ATCAACG113850.030.0120983
GTACATG207550.027.468381
TACATGG202100.026.8602892
CAACGCA137300.025.0573755
ACATGGG214600.024.63873
ACGCAGA139400.024.1741587
AACGCAG141550.024.1390136
TATCAAC146400.024.0777112
CGCAGAG139850.023.995558
GCAGAGT152100.021.5376639
CATGGGG131850.021.38784
CATGGGT30950.020.6525294
CGTCACT4650.020.214921
CGCAGAA6850.019.2115542
CATGGGA59900.018.9881884
GCCCATA23000.018.7998771
CAGAGTA145700.018.61278210-11
Produced by FastQC (version 0.11.5)