##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431375_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3303143
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.509437223880408	33.0	31.0	34.0	26.0	34.0
2	30.627524451711597	33.0	31.0	34.0	26.0	34.0
3	30.802819314816222	34.0	31.0	34.0	26.0	34.0
4	33.820004765158515	37.0	35.0	37.0	27.0	37.0
5	34.16806447677258	37.0	35.0	37.0	30.0	37.0
6	34.03706136852083	37.0	35.0	37.0	30.0	37.0
7	34.04992608554943	37.0	35.0	37.0	30.0	37.0
8	33.92159255593839	37.0	35.0	37.0	28.0	37.0
9	35.29472808170885	39.0	37.0	39.0	27.0	39.0
10-11	35.37342827724988	39.0	37.0	39.0	27.5	39.0
12-13	35.335189393859125	39.0	37.0	39.0	27.5	39.0
14-15	36.649044107384995	40.5	38.0	41.0	27.5	41.0
16-17	36.67068501121507	40.0	38.0	41.0	28.0	41.0
18-19	36.642379091671174	40.0	38.0	41.0	28.0	41.0
20-21	36.664208755115965	40.0	38.0	41.0	28.0	41.0
22-23	36.671237666670805	40.0	38.0	41.0	27.5	41.0
24-25	36.672666608742034	40.0	37.0	41.0	28.0	41.0
26-27	36.66753013720569	40.0	37.0	41.0	28.0	41.0
28-29	36.6379074414883	40.0	37.0	41.0	28.0	41.0
30-31	36.54707395350428	40.0	37.0	41.0	28.0	41.0
32-33	36.44956924359617	40.0	37.0	41.0	27.5	41.0
34-35	36.35616638456162	40.0	36.0	41.0	27.0	41.0
36-37	36.230259634535955	40.0	36.0	41.0	27.0	41.0
38-39	36.09941561718642	40.0	36.0	41.0	26.5	41.0
40-41	35.975310938702926	40.0	35.0	41.0	26.0	41.0
42-43	35.80883343530692	40.0	35.0	41.0	25.5	41.0
44-45	35.63193797543733	40.0	35.0	41.0	25.0	41.0
46-47	35.452881089314026	40.0	35.0	41.0	25.0	41.0
48-49	35.27659020514704	39.0	35.0	41.0	25.0	41.0
50-51	35.07875302401379	39.0	35.0	41.0	25.0	41.0
52-53	34.84332558414819	39.0	34.5	41.0	25.0	41.0
54-55	34.63532051140383	38.5	34.0	41.0	25.0	41.0
56-57	34.38808901098136	38.0	34.0	40.0	25.0	41.0
58-59	34.108118843174516	37.5	33.5	40.0	25.0	41.0
60-61	33.881971655480854	37.0	33.0	40.0	24.5	41.0
62-63	33.679285304935334	36.5	33.0	40.0	24.0	41.0
64-65	33.39624790691774	36.0	33.0	40.0	23.0	41.0
66-67	33.08485524241609	35.5	33.0	39.0	22.5	41.0
68-69	32.773327100885425	35.0	33.0	39.0	22.0	41.0
70-71	32.550753025224765	35.0	32.5	39.0	22.0	41.0
72-73	32.32281436195768	35.0	33.0	38.0	20.5	40.0
74-75	32.00395789706955	35.0	32.0	37.0	20.0	40.0
76-77	31.714453325211778	35.0	32.0	37.0	19.5	39.0
78-79	31.417985082692454	35.0	32.0	36.0	18.0	39.0
80-81	31.139755378438053	35.0	32.0	36.0	17.5	38.5
82-83	30.876084989357103	35.0	31.0	35.5	14.5	37.0
84-85	30.639404803243455	35.0	31.0	35.0	11.0	37.0
86-87	30.424252749578205	35.0	31.0	35.0	7.0	36.5
88-89	30.220169093496708	35.0	31.0	35.0	4.0	36.0
90-91	30.045521795453602	35.0	31.0	35.0	2.0	36.0
92-93	29.879881827701677	35.0	31.0	35.0	2.0	36.0
94-95	29.675330132543458	34.5	30.0	35.0	2.0	35.0
96-97	29.51480847181003	34.0	30.0	35.0	2.0	35.0
98-99	29.417776796221055	34.5	30.0	35.0	2.0	35.0
100	29.38046854162838	35.0	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4429381164001249
1101	2	0.42170971830713455
1101	3	0.41443984565047387
1101	4	0.4823001316917157
1101	5	0.3987914140882438
1101	6	0.39192568727983
1101	7	0.36527567692826324
1101	8	0.26594222589290695
1101	9	0.4551245377311872
1101	10-11	0.443119416263535
1101	12-13	0.45087394678122195
1101	14-15	0.5143003950907925
1101	16-17	0.4586529861462907
1101	18-19	0.4298221836043794
1101	20-21	0.40228223738441926
1101	22-23	0.42932029133881144
1101	24-25	0.39622467227574987
1101	26-27	0.4260528548545679
1101	28-29	0.4592186991185443
1101	30-31	0.49254082166847724
1101	32-33	0.4762038618439135
1101	34-35	0.5516541997080111
1101	36-37	0.49401011351636726
1101	38-39	0.4905773072934494
1101	40-41	0.48806028011696867
1101	42-43	0.5241735464172237
1101	44-45	0.4312365873552153
1101	46-47	0.3985681281446958
1101	48-49	0.4026347360154716
1101	50-51	0.39986029092614217
1101	52-53	0.3838262575354605
1101	54-55	0.4416263869290873
1101	56-57	0.34614260633231453
1101	58-59	0.39493175629867494
1101	60-61	0.37768063836236365
1101	62-63	0.3778918695484066
1101	64-65	0.33786803967710455
1101	66-67	0.3373526234504709
1101	68-69	0.3369712140747083
1101	70-71	0.36052126027728804
1101	72-73	0.41969445312629716
1101	74-75	0.35334880270769276
1101	76-77	0.31857737023196364
1101	78-79	0.2903114686206365
1101	80-81	0.26276533158729976
1101	82-83	0.25695030484903825
1101	84-85	0.3275324261773278
1101	86-87	0.33436285423705314
1101	88-89	0.2881507054801453
1101	90-91	0.26818984478382646
1101	92-93	0.22521532448491755
1101	94-95	0.32936575250663225
1101	96-97	0.29759720573318305
1101	98-99	0.3613400582616535
1101	100	0.4301621304121177
1102	1	0.2962437269883438
1102	2	0.327695483371393
1102	3	0.32977985874535776
1102	4	0.3286001013144926
1102	5	0.246335760596736
1102	6	0.26157835823642017
1102	7	0.28635468554159615
1102	8	0.29737308987677125
1102	9	0.3016283130994992
1102	10-11	0.3419756109779044
1102	12-13	0.33832488635717084
1102	14-15	0.4656808643523078
1102	16-17	0.5044832585609953
1102	18-19	0.34605802219119397
1102	20-21	0.39426434507954156
1102	22-23	0.29426025699197567
1102	24-25	0.33806100561190533
1102	26-27	0.48257203667858306
1102	28-29	0.46225642133518363
1102	30-31	0.4803338983243748
1102	32-33	0.4693055698667763
1102	34-35	0.4443000073487866
1102	36-37	0.40591435386191677
1102	38-39	0.37824923813504086
1102	40-41	0.4009918999006459
1102	42-43	0.40563194354071186
1102	44-45	0.4364412584279975
1102	46-47	0.4163794674413239
1102	48-49	0.2998146163703552
1102	50-51	0.34775318882842043
1102	52-53	0.45391994282501713
1102	54-55	0.3375978347607429
1102	56-57	0.4218939584817747
1102	58-59	0.3776253324238752
1102	60-61	0.2721980943728681
1102	62-63	0.30491319532657
1102	64-65	0.2938841214511285
1102	66-67	0.27287811913775073
1102	68-69	0.26538831875025437
1102	70-71	0.3088960788913866
1102	72-73	0.33776309112542435
1102	74-75	0.3450195136194125
1102	76-77	0.3462472678570947
1102	78-79	0.33993683638471595
1102	80-81	0.3760807209785071
1102	82-83	0.34644414287187786
1102	84-85	0.3508080347202238
1102	86-87	0.3510803714861126
1102	88-89	0.31265628895880937
1102	90-91	0.2554501703163048
1102	92-93	0.30217184148829546
1102	94-95	0.27767969565160655
1102	96-97	0.1997818242085856
1102	98-99	0.22503346858288964
1102	100	0.24059253087104437
1103	1	-0.027722287992531847
1103	2	0.03177387909279972
1103	3	0.06814985444161792
1103	4	0.012580768345443971
1103	5	0.004989899679991083
1103	6	0.041224581344110334
1103	7	0.021856893020434143
1103	8	-0.005854713234491271
1103	9	0.044817527643232324
1103	10-11	0.10574551576375057
1103	12-13	0.13974173476406548
1103	14-15	0.10276614605368906
1103	16-17	0.0677530352018465
1103	18-19	0.09702781382259928
1103	20-21	0.06959933543240027
1103	22-23	0.07391288734928736
1103	24-25	0.07753329002608922
1103	26-27	0.11338462100040658
1103	28-29	0.11020090312167241
1103	30-31	0.1082290080213184
1103	32-33	0.13782711515030854
1103	34-35	0.24303644936680513
1103	36-37	0.18262375970436295
1103	38-39	0.14431330095993644
1103	40-41	0.09623228056381095
1103	42-43	0.0682838437133455
1103	44-45	0.05228324356318126
1103	46-47	-0.011198906519616969
1103	48-49	0.00572693618157416
1103	50-51	-0.03515335720464208
1103	52-53	-0.10630167547699187
1103	54-55	-0.13545786720784747
1103	56-57	-0.26525346145177764
1103	58-59	-0.21257544418313046
1103	60-61	-0.205880649516061
1103	62-63	-0.1983976602467692
1103	64-65	-0.17308167108937766
1103	66-67	-0.24583467650241886
1103	68-69	-0.25909487007743337
1103	70-71	-0.1662767959568967
1103	72-73	-0.2055917447764557
1103	74-75	-0.16501242591549214
1103	76-77	-0.17255498749477738
1103	78-79	-0.2430837103350143
1103	80-81	-0.20961068170141317
1103	82-83	-0.2113787462415999
1103	84-85	-0.17838605416887887
1103	86-87	-0.1647203896467886
1103	88-89	-0.11506389073432288
1103	90-91	-0.11454854823377048
1103	92-93	-0.05542691477992534
1103	94-95	-0.11922620723866473
1103	96-97	-0.12765329332213327
1103	98-99	-0.15990864856581055
1103	100	-0.27184720471506196
1104	1	0.08620109235405593
1104	2	0.14613388630178648
1104	3	0.14748885711154713
1104	4	0.0693577746458729
1104	5	0.08007411292484079
1104	6	0.11959817415147
1104	7	0.1263847135835121
1104	8	0.158041447607701
1104	9	0.1440607696812961
1104	10-11	0.11911080337079483
1104	12-13	0.16264829475752407
1104	14-15	0.12062023650419462
1104	16-17	0.11188133289590496
1104	18-19	0.03547410964751663
1104	20-21	0.08875471328185114
1104	22-23	0.08110139124269011
1104	24-25	0.1840025208381988
1104	26-27	0.19001941700276603
1104	28-29	0.2104019837267046
1104	30-31	0.24361394569515937
1104	32-33	0.24901284506965737
1104	34-35	0.23723878782880092
1104	36-37	0.2376976889931015
1104	38-39	0.31295234431976837
1104	40-41	0.24800893384677636
1104	42-43	0.2568802184097905
1104	44-45	0.22603737061601947
1104	46-47	0.202179944389961
1104	48-49	0.1769277715323696
1104	50-51	0.19468837020270513
1104	52-53	0.16780226401210285
1104	54-55	0.12348044206145659
1104	56-57	0.15755987406159022
1104	58-59	0.14579144129243105
1104	60-61	0.17024803092324703
1104	62-63	0.16001442075400973
1104	64-65	0.2474707733707504
1104	66-67	0.29341501274931403
1104	68-69	0.275183590097555
1104	70-71	0.27752770111398917
1104	72-73	0.2521928476834603
1104	74-75	0.2749002394220952
1104	76-77	0.2605905653212801
1104	78-79	0.26772094652272216
1104	80-81	0.25962194194295307
1104	82-83	0.2741255206247395
1104	84-85	0.2520128369846901
1104	86-87	0.31266521876023035
1104	88-89	0.30641248403644994
1104	90-91	0.2777686305249816
1104	92-93	0.25642731873555036
1104	94-95	0.21402404312503265
1104	96-97	0.27971808290715927
1104	98-99	0.34292547781815585
1104	100	0.3361792976064919
1105	1	0.21591241402906292
1105	2	0.15883456721599742
1105	3	0.2676778389055201
1105	4	0.2392232470557829
1105	5	0.16565219977896817
1105	6	0.2791393228779242
1105	7	0.3001395225223149
1105	8	0.24843350519245178
1105	9	0.3169738908292814
1105	10-11	0.2760640470533815
1105	12-13	0.34384962348090653
1105	14-15	0.371056268094236
1105	16-17	0.35919637365820734
1105	18-19	0.3682241144956393
1105	20-21	0.32533561535095856
1105	22-23	0.30250925790787875
1105	24-25	0.32793618797300894
1105	26-27	0.36655212651781
1105	28-29	0.30922075330929744
1105	30-31	0.35874765393095487
1105	32-33	0.39021810375263044
1105	34-35	0.35772403306388867
1105	36-37	0.3568569864494151
1105	38-39	0.3904864904390877
1105	40-41	0.39524910685690884
1105	42-43	0.38061636555497813
1105	44-45	0.3409466869822708
1105	46-47	0.33354366885935605
1105	48-49	0.34393278756148504
1105	50-51	0.34416423619769176
1105	52-53	0.39292348754932505
1105	54-55	0.3183979823001124
1105	56-57	0.3134836401981147
1105	58-59	0.3560576978928651
1105	60-61	0.3900412687274297
1105	62-63	0.3815248560558615
1105	64-65	0.38045112568666895
1105	66-67	0.38628709909004755
1105	68-69	0.3672801619614461
1105	70-71	0.35543032689866294
1105	72-73	0.37087491606641265
1105	74-75	0.35365654691073445
1105	76-77	0.34317929569342454
1105	78-79	0.37703353997529376
1105	80-81	0.3471975208284803
1105	82-83	0.4057387047017116
1105	84-85	0.33197894429997277
1105	86-87	0.32380508258474094
1105	88-89	0.32268588055365655
1105	90-91	0.37657317744043795
1105	92-93	0.2652784590166348
1105	94-95	0.3646324837701904
1105	96-97	0.3449533901567463
1105	98-99	0.3196389048155268
1105	100	0.36641802059387985
1106	1	0.18533823456496634
1106	2	0.19777331693938294
1106	3	0.20459473319773736
1106	4	0.1981875831859412
1106	5	0.21083664545092518
1106	6	0.20079953381937798
1106	7	0.2707754044171722
1106	8	0.23731096353430559
1106	9	0.20182548798076994
1106	10-11	0.2569916443918103
1106	12-13	0.23515300427634855
1106	14-15	0.3566067615503812
1106	16-17	0.3075837937499557
1106	18-19	0.25175922795367
1106	20-21	0.2309975650869589
1106	22-23	0.31671410569860114
1106	24-25	0.2783354969598406
1106	26-27	0.2866238855776473
1106	28-29	0.2330913392458953
1106	30-31	0.2521625889823653
1106	32-33	0.2979572597953606
1106	34-35	0.27263233811646614
1106	36-37	0.2805437263954573
1106	38-39	0.2559464179255002
1106	40-41	0.2731939508074177
1106	42-43	0.34617044020714616
1106	44-45	0.30651793560905105
1106	46-47	0.2851195358776124
1106	48-49	0.28339959477320065
1106	50-51	0.22062620597937865
1106	52-53	0.2231947856709482
1106	54-55	0.24427370015817473
1106	56-57	0.18448006294366337
1106	58-59	0.18807370291105485
1106	60-61	0.23988892712637266
1106	62-63	0.24776605861697476
1106	64-65	0.2775024387074154
1106	66-67	0.3428589034779108
1106	68-69	0.34333372899597236
1106	70-71	0.3568267681132724
1106	72-73	0.2411227224430803
1106	74-75	0.27652315918376047
1106	76-77	0.3047833561841031
1106	78-79	0.30740151912777947
1106	80-81	0.2492411111914592
1106	82-83	0.30001965853863055
1106	84-85	0.32671864033751774
1106	86-87	0.33580940930018244
1106	88-89	0.31252930458246553
1106	90-91	0.33041910605413705
1106	92-93	0.3058982874683345
1106	94-95	0.3056798597276398
1106	96-97	0.35366622609799236
1106	98-99	0.29937671171585833
1106	100	0.2771229249742575
1107	1	-0.0870564425973086
1107	2	-0.069684917844274
1107	3	-0.09806759213370597
1107	4	-0.166371016295642
1107	5	-0.10059865016608427
1107	6	-0.15626398654030282
1107	7	-0.12224422937437396
1107	8	-0.14884947014868288
1107	9	-0.16527462174838092
1107	10-11	-0.14660307976804177
1107	12-13	-0.12459160341368403
1107	14-15	-0.09301081035765435
1107	16-17	-0.06604370951979632
1107	18-19	-0.1714321996334789
1107	20-21	-0.14643003371315189
1107	22-23	-0.06329222647602961
1107	24-25	-0.09540292351360335
1107	26-27	-0.023103639975836643
1107	28-29	0.02426001158335822
1107	30-31	-0.007278382139318751
1107	32-33	0.08561951357354047
1107	34-35	0.04338738475280479
1107	36-37	0.05803415320509231
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2216	62-63	-0.6628259735738311
2216	64-65	-0.6867535673064395
2216	66-67	-0.7026534365615404
2216	68-69	-0.747960515990755
2216	70-71	-0.7687393681752894
2216	72-73	-0.7257994593161854
2216	74-75	-0.7149478903287303
2216	76-77	-0.7220596429190529
2216	78-79	-0.6873779926106707
2216	80-81	-0.714112878627148
2216	82-83	-0.6640565085505301
2216	84-85	-0.6194360474508898
2216	86-87	-0.6095691205183797
2216	88-89	-0.6972828000185842
2216	90-91	-0.7064702364789994
2216	92-93	-0.7525777722527636
2216	94-95	-0.7573510875297984
2216	96-97	-0.8817378313673956
2216	98-99	-0.9354870389353565
2216	100	-0.8821467413166744
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	75372.0
3	20374.0
4	16147.0
5	14917.0
6	8687.0
7	7919.0
8	8433.0
9	8308.0
10	8310.0
11	9003.0
12	10280.0
13	11630.0
14	11364.0
15	10687.0
16	10004.0
17	10081.0
18	10080.0
19	9425.0
20	8927.0
21	9794.0
22	12614.0
23	26855.0
24	49992.0
25	42260.0
26	48324.0
27	33509.0
28	33751.0
29	34816.0
30	40013.0
31	48963.0
32	66019.0
33	87676.0
34	128633.0
35	231187.0
36	407429.0
37	687140.0
38	752605.0
39	290203.0
40	1412.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.131230522285804	22.44786315057155	12.908195671141407	22.51271065600124
2	15.721192562228667	23.63884061832075	37.12627984281623	23.513686976634354
3	17.56206666258974	30.719660250755187	28.090345495289032	23.62792759136604
4	12.088826968028311	20.555192769548743	36.55911146390663	30.796868798516318
5	12.811468595658315	42.24035781689071	31.64002637488791	13.308147212563068
6	27.924404120560325	40.61492342293385	17.78999577069476	13.67067668581106
7	25.62111298239283	36.71388129427034	21.13284226568453	16.532163457652302
8	24.26649406338145	38.55001130741236	19.189935161753517	17.993559467452666
9	26.0332658925151	20.4314496829232	19.56472971348803	33.97055471107366
10-11	23.6884688310497	30.450604167000943	25.93137808444866	19.929548917500696
12-13	24.100848797645153	28.866718758467314	25.734610944788038	21.297821499099495
14-15	22.173381533890602	29.579797181048473	23.817013069067855	24.42980821599307
16-17	21.76496748702675	32.111083292488395	24.42360200572606	21.700347214758793
18-19	21.805005111798067	31.071028411425118	26.112947577504215	21.011018899272603
20-21	23.78673887264342	29.54237827426787	25.38975757331729	21.281125279771416
22-23	23.393855488545302	29.920230519841258	25.17726298861418	21.508651002999265
24-25	22.29750573923079	30.052301701742856	25.609351457081935	22.04084110194442
26-27	22.33139770212794	30.3549074321033	25.60070817400276	21.71298669176599
28-29	22.14675537813531	30.087798197050507	26.06179932264513	21.703647102169054
30-31	22.531661511475583	30.28794090961245	25.486059792143422	21.694337786768543
32-33	21.927978897674123	30.11138179606514	25.85726685160164	22.10337245465909
34-35	22.629265520747964	29.596266343903366	26.0520964426911	21.722371692657568
36-37	22.43647943791716	29.906364937878866	25.722970516262844	21.934185107941133
38-39	22.319257749361746	29.84984301315444	25.723454903405635	22.10744433407818
40-41	22.35050072007176	29.648262276262336	25.84977398798659	22.151463015679308
42-43	22.092731074615905	30.17566905217243	26.06908026688521	21.66251960632646
44-45	22.12691064237909	29.93698728756218	25.686792851535646	22.249309218523088
46-47	22.320574676906208	29.813816719409363	25.850878996156084	22.014729607528345
48-49	22.059232676272266	29.797862217893684	25.790799853351793	22.352105252482257
50-51	22.09023345341089	29.94947539358726	25.746069728134692	22.214221424867166
52-53	22.314353329540985	30.016744658042356	25.462748660896604	22.20615335152005
54-55	22.214145739376104	29.86771992614307	25.73379354148458	22.184340792996245
56-57	22.178543284380968	30.014065391658796	25.569132187132077	22.238259136828166
58-59	22.608164405840135	29.47146096914363	25.67230664854655	22.248067976469684
60-61	22.491926628668512	29.95636277327382	25.495581026918906	22.05612957113876
62-63	22.005374880833195	29.85040308578829	25.82865773598055	22.315564297397962
64-65	22.59761384838622	29.73168282450987	25.48898125209838	22.181722075005535
66-67	22.21685527995609	29.72570367071604	25.531062385128344	22.526378664199523
68-69	22.352771284803595	29.855277231412625	25.748098099295124	22.043853384488653
70-71	22.38496789270098	29.76151804508615	25.647829960737393	22.205684101475473
72-73	22.489050580008193	29.823307679988424	25.454968192415528	22.232673547587858
74-75	22.349713590964726	29.718498411967026	25.630255789713008	22.301532207355237
76-77	22.478272966081093	29.604152772071934	25.18566407812196	22.731910183725017
78-79	22.652289047128747	29.437644691737535	25.62308080516042	22.286985455973294
80-81	22.071054749976007	30.12144796637627	25.625366506990467	22.182130776657264
82-83	22.204296517858765	30.102917224216956	25.608708683252907	22.084077574671372
84-85	22.169768551098482	29.43482485457339	25.708234304680733	22.6871722896474
86-87	22.63066800197632	29.86890512681405	25.346189523551182	22.154237347658444
88-89	22.21049398517035	29.784930236331963	25.523331580909677	22.481244197588012
90-91	22.175487864910963	29.771687880290614	25.291138596685624	22.761685658112796
92-93	22.201470565529235	29.770172290554008	25.69002958750961	22.33832755640715
94-95	22.399311067559186	29.382396407718065	25.76893727871989	22.44935524600286
96-97	22.22339116151712	30.181183404203853	25.33385789273951	22.26156754153952
98-99	21.75313056867633	30.006064048216903	25.617894119235597	22.62291126387117
100	21.799005104056572	29.92946841094876	25.609351815998444	22.662174668996222
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	64427.0
1	46580.5
2	23047.0
3	12758.0
4	5909.5
5	3076.0
6	2575.5
7	3527.5
8	5080.5
9	5405.0
10	3418.0
11	1533.5
12	1239.5
13	1179.0
14	1144.0
15	1166.0
16	1256.5
17	1526.0
18	1912.5
19	2345.0
20	3164.0
21	4351.0
22	5724.5
23	7676.5
24	10581.5
25	13984.0
26	17712.5
27	22357.5
28	28171.5
29	34424.0
30	39096.5
31	42739.5
32	47271.0
33	52327.5
34	57315.0
35	62867.5
36	67711.5
37	73108.0
38	80177.0
39	84615.0
40	89526.5
41	94629.5
42	97403.0
43	103100.0
44	105714.0
45	105482.5
46	108836.5
47	109053.5
48	109468.0
49	115182.5
50	120658.0
51	118316.5
52	113315.0
53	109806.5
54	107432.5
55	106878.0
56	95158.0
57	81016.0
58	78225.5
59	76656.5
60	69742.0
61	59655.0
62	50496.0
63	39306.5
64	27998.5
65	22047.0
66	17684.0
67	13512.0
68	10010.0
69	7228.5
70	5692.5
71	5560.0
72	4504.0
73	2737.5
74	2104.5
75	1725.5
76	1292.5
77	938.5
78	655.5
79	453.0
80	319.0
81	206.5
82	146.0
83	127.0
84	124.5
85	110.5
86	87.0
87	79.0
88	74.0
89	61.0
90	46.5
91	42.0
92	42.5
93	33.5
94	26.0
95	16.5
96	13.5
97	17.5
98	13.5
99	15.0
100	41.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.7246776781992177E-4
2	9.082258927330728E-5
3	3.0274196424435756E-5
4	6.054839284887151E-5
5	3.0274196424435756E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	9.082258927330728E-5
84-85	4.9952424100319E-4
86-87	0.0011806936605529945
88-89	0.002982008347806922
90-91	0.0023311131246815534
92-93	0.0018164517854661454
94-95	0.001861863080102799
96-97	0.001619669508707313
98-99	0.002088919553286067
100	0.0025430324996526033
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3303143.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.427714027146656
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.35949116007774	26.952584685008045
2	14.58624649322425	11.50204710928633
3	6.154707728759695	7.279981687506195
4	3.319875625403697	5.235804270564464
5	1.9300743104780194	3.8049208982334806
6	1.2595354119236652	2.9796361217034377
7	0.8865576547545739	2.446845917616881
8	0.6150210965868127	1.9399100733509596
9	0.4636465481675123	1.6452471159740116
>10	2.154343531062601	14.686763783221368
>50	0.14599946167169683	4.02559790234778
>100	0.10821646692051123	8.512896145929247
>500	0.011061177262332593	2.8907889819695005
>1k	0.0049928925138628165	3.542632368436556
>5k	1.5362746196500974E-4	0.43953924036418435
>10k+	7.681373098250487E-5	2.1148036984875813
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	69828	2.1139865879255	No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9440	0.28578841424667356	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5073	0.1535809984611626	No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4338	0.13132946408920232	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4165	0.12609202810777492	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	3960	0.1198858178407656	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3902	0.11812991444814833	No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG	3503	0.10605051007479846	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	3368	0.10196349355749963	No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA	3332	0.10087362248621995	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	3.0274196424435756E-5	0.0
8	0.0	0.0	0.0	3.0274196424435756E-5	0.0
9	0.0	0.0	0.0	3.0274196424435756E-5	0.0
10-11	0.0	0.0	0.0	3.0274196424435756E-5	0.0
12-13	0.0	0.0	0.0	1.6650808033439666E-4	0.0
14-15	0.0	0.0	0.0	1.8164517854661453E-4	0.0
16-17	0.0	0.0	0.0	1.8164517854661453E-4	0.0
18-19	0.0	0.0	0.0	4.087016517298827E-4	0.0
20-21	0.0	0.0	0.0	6.054839284887151E-4	0.0
22-23	0.0	0.0	0.0	0.0012109678569774303	0.0
24-25	0.0	0.0	0.0	0.0018921372765272348	0.0
26-27	0.0	0.0	0.0	0.0027852260710480893	0.0
28-29	0.0	0.0	0.0	0.005146613392154078	0.0
30-31	0.0	0.0	0.0	0.010838162319948	0.0
32-33	0.0	0.0	0.0	0.019541993791973283	0.0
34-35	0.0	0.0	0.0	0.031137011022532177	0.0
36-37	0.0	0.0	0.0	0.04874145624334157	0.0
38-39	0.0	0.0	0.0	0.07343006342746893	0.0
40-41	0.0	0.0	0.0	0.10004108208454796	0.0
42-43	0.0	0.0	0.0	0.1240333827509133	0.0
44-45	0.0	0.0	0.0	0.15345990167546486	0.0
46-47	0.0	0.0	0.0	0.18933482443842123	0.0
48-49	0.0	0.0	0.0	0.22976601376325517	0.0
50-51	0.0	0.0	0.0	0.2727856468823784	0.0
52-53	0.0	0.0	0.0	0.3160777477693215	0.0
54-55	0.0	0.0	0.0	0.36088355847748643	0.0
56-57	0.0	0.0	0.0	0.4168151363716315	0.0
58-59	0.0	0.0	0.0	0.48001252140764117	0.0
60-61	0.0	0.0	0.0	0.5509298265318819	0.0
62-63	0.0	0.0	0.0	0.6239965996022576	0.0
64-65	0.0	0.0	0.0	0.6963519290566591	0.0
66-67	0.0	0.0	0.0	0.778046848107999	0.0
68-69	0.0	0.0	0.0	0.8782695753710935	0.0
70-71	0.0	0.0	0.0	0.9939170057124382	0.0
72-73	0.0	0.0	0.0	1.1180714852490492	0.0
74-75	0.0	0.0	0.0	1.2446933117942516	0.0
76-77	0.0	0.0	0.0	1.3779754615528301	0.0
78-79	0.0	0.0	0.0	1.5311780325586875	0.0
80-81	0.0	0.0	0.0	1.7064959040525949	0.0
82-83	0.0	0.0	0.0	1.9006594628207134	0.0
84-85	0.0	0.0	0.0	2.096185360427932	0.0
86-87	0.0	0.0	0.0	2.3028370252211303	0.0
88	0.0	0.0	0.0	2.4691029119841317	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5180	0.0	47.90702	1
GTATCAA	9270	0.0	38.32984	1
TCAACGC	11255	0.0	30.316992	4
ATCAACG	11385	0.0	30.012098	3
GTACATG	20755	0.0	27.46838	1
TACATGG	20210	0.0	26.860289	2
CAACGCA	13730	0.0	25.057375	5
ACATGGG	21460	0.0	24.6387	3
ACGCAGA	13940	0.0	24.174158	7
AACGCAG	14155	0.0	24.139013	6
TATCAAC	14640	0.0	24.077711	2
CGCAGAG	13985	0.0	23.99555	8
GCAGAGT	15210	0.0	21.537663	9
CATGGGG	13185	0.0	21.3878	4
CATGGGT	3095	0.0	20.652529	4
CGTCACT	465	0.0	20.21492	1
CGCAGAA	685	0.0	19.211554	2
CATGGGA	5990	0.0	18.988188	4
GCCCATA	2300	0.0	18.799877	1
CAGAGTA	14570	0.0	18.612782	10-11
>>END_MODULE