FastQCFastQC Report
Wed 25 May 2016
SRR2431375_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431375_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3303143
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT468711.4189818606097284No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT316350.9577242038870252No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT294050.8902127458605333No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA152610.4620145116333141No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100160.30322635138714854No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91600.27731163924783153No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60100.1819479205108589No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48750.14758670756912431No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40770.12342789882242458No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105000.062.6300851
GTATCAA226400.049.5391541
ATCAACG316650.035.3555563
TCAACGC319750.035.0127834
TATCAAC332300.033.7187422
CAACGCA340550.032.858995
AACGCAG349450.032.0759126
ACGCAGA375750.029.743267
CGCAGAG378900.029.4587758
GCAGAGT398300.027.8469339
GTGGTAT34950.026.8990781
TGGTATC36500.025.4957472
GTACATG197350.025.2716061
TACATGG193750.024.7188282
TAACGCA2950.023.8981724
AGAGTAC391850.023.50179910-11
AGTACTT297750.023.3685812-13
ACATGGG203500.023.003313
TATAACG3150.022.3808252
GAGTACT295650.022.302612-13
Produced by FastQC (version 0.11.5)