##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431375_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3303143
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.71465903837648	34.0	31.0	34.0	31.0	34.0
2	32.83592990070366	34.0	31.0	34.0	31.0	34.0
3	32.908133253692014	34.0	31.0	34.0	31.0	34.0
4	36.30818435653558	37.0	37.0	37.0	35.0	37.0
5	36.26982059208457	37.0	37.0	37.0	35.0	37.0
6	36.30863180915873	37.0	37.0	37.0	35.0	37.0
7	36.17563332862065	37.0	37.0	37.0	35.0	37.0
8	36.23022194316141	37.0	37.0	37.0	35.0	37.0
9	38.03794961344392	39.0	39.0	39.0	35.0	39.0
10-11	38.02501254108587	39.0	39.0	39.0	35.0	39.0
12-13	37.990358576664704	39.0	39.0	39.0	35.0	39.0
14-15	39.40203027843481	41.0	39.0	41.0	36.0	41.0
16-17	39.42462663590405	41.0	39.0	41.0	36.0	41.0
18-19	39.505590887224685	41.0	39.5	41.0	36.0	41.0
20-21	39.49525906083993	41.0	40.0	41.0	36.0	41.0
22-23	39.40004156647169	41.0	39.0	41.0	36.0	41.0
24-25	39.33451957726324	41.0	39.0	41.0	35.5	41.0
26-27	39.20400645688061	41.0	39.0	41.0	35.0	41.0
28-29	39.090689231438056	41.0	39.0	41.0	35.0	41.0
30-31	38.82795143897797	41.0	38.5	41.0	35.0	41.0
32-33	38.53044585111816	40.5	38.0	41.0	35.0	41.0
34-35	38.3821829693719	40.0	38.0	41.0	35.0	41.0
36-37	38.23220172423659	40.0	38.0	41.0	35.0	41.0
38-39	38.08094835736751	40.0	37.0	41.0	34.0	41.0
40-41	37.930471220894766	40.0	37.0	41.0	34.0	41.0
42-43	37.726142949306166	40.0	37.0	41.0	33.5	41.0
44-45	37.41462434414738	40.0	36.5	41.0	33.0	41.0
46-47	36.96011177838804	40.0	36.0	41.0	32.0	41.0
48-49	36.57979930024223	40.0	36.0	41.0	31.0	41.0
50-51	36.36769177114039	40.0	35.0	41.0	30.5	41.0
52-53	36.21203290320764	40.0	35.0	41.0	30.0	41.0
54-55	36.02710539628469	39.0	35.0	41.0	30.0	41.0
56-57	35.81280843729745	39.0	35.0	41.0	30.0	41.0
58-59	35.57727640008319	39.0	35.0	41.0	29.5	41.0
60-61	35.340323746201726	38.0	35.0	41.0	29.0	41.0
62-63	35.08582310847578	37.0	35.0	41.0	29.0	41.0
64-65	34.796910548529084	37.0	35.0	40.0	28.5	41.0
66-67	34.50726822907758	36.0	35.0	40.0	28.0	41.0
68-69	34.2418852287049	36.0	35.0	39.0	28.0	41.0
70-71	34.007793486385545	35.0	35.0	39.0	28.0	41.0
72-73	33.77425606460271	35.0	35.0	39.0	28.0	41.0
74-75	33.511502680931464	35.0	35.0	37.5	28.0	40.0
76-77	33.24862320523211	35.0	34.0	37.0	28.0	39.0
78-79	32.98367312586831	35.0	34.0	36.5	27.0	39.0
80-81	32.7365392597293	35.0	34.0	36.0	27.0	39.0
82-83	32.48464462483156	35.0	34.0	36.0	26.5	37.0
84-85	32.28036585155411	35.0	34.0	35.5	26.0	37.0
86-87	32.1106870032572	35.0	34.0	35.0	26.0	37.0
88-89	31.933468366340783	35.0	34.0	35.0	25.5	36.0
90-91	31.766386589984144	35.0	34.0	35.0	25.0	36.0
92-93	31.645897256037657	35.0	34.0	35.0	25.0	36.0
94-95	31.5300179253517	35.0	34.0	35.0	25.0	36.0
96-97	31.415702711024018	35.0	34.0	35.0	24.0	35.0
98-99	31.306715149783102	35.0	34.0	35.0	24.0	35.0
100	31.202841051689255	35.0	34.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2115110605637227
1101	2	0.17038408853657927
1101	3	0.21888795700798624
1101	4	0.13318703062780912
1101	5	0.1722192663758264
1101	6	0.17763541363814284
1101	7	0.1689058165128401
1101	8	0.17728485983924003
1101	9	0.2367818680483751
1101	10-11	0.26202340349324516
1101	12-13	0.2143300488040083
1101	14-15	0.3263223371369506
1101	16-17	0.34026359400265704
1101	18-19	0.2786456550123404
1101	20-21	0.2667919890716277
1101	22-23	0.3052338581134464
1101	24-25	0.34885783516740076
1101	26-27	0.3373919017320972
1101	28-29	0.35755633408981424
1101	30-31	0.40987865842281224
1101	32-33	0.4336912614999733
1101	34-35	0.45486425826224774
1101	36-37	0.4257439416162043
1101	38-39	0.4266684043058291
1101	40-41	0.43490620251048995
1101	42-43	0.46333007473734966
1101	44-45	0.5392784328099296
1101	46-47	0.5323703304911334
1101	48-49	0.5026712299317282
1101	50-51	0.5218089631531413
1101	52-53	0.4803380265883277
1101	54-55	0.46151140830016146
1101	56-57	0.5056446476907723
1101	58-59	0.56599893490894
1101	60-61	0.5291012187468169
1101	62-63	0.475815958001057
1101	64-65	0.5201623456162281
1101	66-67	0.5589587887716121
1101	68-69	0.4909451730250112
1101	70-71	0.36932498882780607
1101	72-73	0.36264105509023636
1101	74-75	0.3107236293573905
1101	76-77	0.2954360374365095
1101	78-79	0.3303068549548911
1101	80-81	0.30347108112788845
1101	82-83	0.3220896800782498
1101	84-85	0.2850006480490066
1101	86-87	0.27176789382434663
1101	88-89	0.2260719734740455
1101	90-91	0.3145206899136994
1101	92-93	0.3658765184413042
1101	94-95	0.32735257589466826
1101	96-97	0.3120719467993993
1101	98-99	0.28812907847142455
1101	100	0.405895349031546
1102	1	0.1320943516392319
1102	2	0.10969160889597163
1102	3	0.16094443443626005
1102	4	0.11122030518343706
1102	5	0.15092277621574368
1102	6	0.10475380129748402
1102	7	0.18380178965502125
1102	8	0.19409447695311854
1102	9	0.20037373857121565
1102	10-11	0.22994602508383366
1102	12-13	0.23070107208070567
1102	14-15	0.30889488847263635
1102	16-17	0.33506194854962246
1102	18-19	0.3291134763361754
1102	20-21	0.3184673957451736
1102	22-23	0.3030339905145638
1102	24-25	0.3094041168622823
1102	26-27	0.2914736442954293
1102	28-29	0.2986562315818233
1102	30-31	0.2914356907497506
1102	32-33	0.28430915356280195
1102	34-35	0.3041718149290844
1102	36-37	0.26834091575441477
1102	38-39	0.2768339057054803
1102	40-41	0.3173330399996104
1102	42-43	0.3705879313641276
1102	44-45	0.2888969021610066
1102	46-47	0.373246211097765
1102	48-49	0.3457802599543456
1102	50-51	0.311771167111381
1102	52-53	0.26401141940937833
1102	54-55	0.2808690813323196
1102	56-57	0.2159870631277272
1102	58-59	0.278395867172577
1102	60-61	0.2806905767950312
1102	62-63	0.3160285017363833
1102	64-65	0.36058789298109417
1102	66-67	0.37984270542425946
1102	68-69	0.3361032737587806
1102	70-71	0.34383244819071024
1102	72-73	0.36301320631797296
1102	74-75	0.3146674641730769
1102	76-77	0.32509582465072384
1102	78-79	0.23914977324984932
1102	80-81	0.2497384950822905
1102	82-83	0.3084608364822117
1102	84-85	0.28505159948539216
1102	86-87	0.3102690260112482
1102	88-89	0.3396279601468031
1102	90-91	0.40943655953127944
1102	92-93	0.4056371556126166
1102	94-95	0.3812708139673724
1102	96-97	0.380804829355732
1102	98-99	0.4064082996042906
1102	100	0.43256582394492327
1103	1	0.12017058098491873
1103	2	0.16000665215095466
1103	3	0.13326390715587877
1103	4	0.10119042080407326
1103	5	0.12348548263798165
1103	6	0.13237243212868322
1103	7	0.10639762034888633
1103	8	0.13827427646664603
1103	9	0.15298015936068765
1103	10-11	0.13360311391974022
1103	12-13	0.17542971901465876
1103	14-15	0.2199962342368451
1103	16-17	0.3230998552890796
1103	18-19	0.21233953860981103
1103	20-21	0.1835530836420034
1103	22-23	0.3076281093847584
1103	24-25	0.25644529734361754
1103	26-27	0.3077026031294494
1103	28-29	0.3290712037525978
1103	30-31	0.27744431273168857
1103	32-33	0.2752421699992169
1103	34-35	0.2539334168865608
1103	36-37	0.18744709234305645
1103	38-39	0.2358921850809068
1103	40-41	0.20134090761858658
1103	42-43	0.23502938844521992
1103	44-45	0.19208747068956455
1103	46-47	0.22929707257060272
1103	48-49	0.024607443133987772
1103	50-51	0.047815452086503285
1103	52-53	0.012944431010126323
1103	54-55	0.08024346780795355
1103	56-57	0.03095355200328953
1103	58-59	-0.007956570241887562
1103	60-61	-0.037391648769173
1103	62-63	-0.015446809801012762
1103	64-65	0.00857855532145635
1103	66-67	0.03399327337253766
1103	68-69	0.019094685626456
1103	70-71	-0.014684841711947172
1103	72-73	-0.009980357418442054
1103	74-75	0.009372537264077607
1103	76-77	-0.03512011175067897
1103	78-79	-0.060013657554272015
1103	80-81	-0.08104169031691555
1103	82-83	-0.012496189006554914
1103	84-85	-0.0823599200240821
1103	86-87	-0.11125123298670658
1103	88-89	-0.12421726004498268
1103	90-91	-0.11530630424334376
1103	92-93	-0.06299959927260801
1103	94-95	-0.14464094974293573
1103	96-97	-0.16088629769156526
1103	98-99	-0.10252280191071961
1103	100	-0.04215068548219847
1104	1	0.10248719305774756
1104	2	0.14935890226568205
1104	3	0.1456374479396203
1104	4	0.06006286444669229
1104	5	0.0791768673396902
1104	6	0.1027199871111577
1104	7	0.08202464439309409
1104	8	0.11375191838431675
1104	9	0.1677321088531798
1104	10-11	0.19290405573317315
1104	12-13	0.19295712896268924
1104	14-15	0.22656928005293508
1104	16-17	0.26763017484420004
1104	18-19	0.2512664026813667
1104	20-21	0.22395105509217927
1104	22-23	0.26183737813695274
1104	24-25	0.2615337189282201
1104	26-27	0.275080259353615
1104	28-29	0.24153964120440463
1104	30-31	0.2567091352846802
1104	32-33	0.21497396033657168
1104	34-35	0.22016218706661306
1104	36-37	0.32158481002941386
1104	38-39	0.34133836199467993
1104	40-41	0.3388289003798661
1104	42-43	0.27094631414619386
1104	44-45	0.25226357066410543
1104	46-47	0.18651683708937838
1104	48-49	0.16136859151837513
1104	50-51	0.21499235285329377
1104	52-53	0.19669596617159613
1104	54-55	0.20957192640364752
1104	56-57	0.20932672676364916
1104	58-59	0.2251341255183661
1104	60-61	0.2542734231256176
1104	62-63	0.2825884622175181
1104	64-65	0.3327218376778305
1104	66-67	0.36047325263740504
1104	68-69	0.3369531423455925
1104	70-71	0.28753424464169797
1104	72-73	0.3301589358406005
1104	74-75	0.3052099504264234
1104	76-77	0.2947376872411809
1104	78-79	0.29437639329881904
1104	80-81	0.26524196478873563
1104	82-83	0.3061846590528745
1104	84-85	0.3458140321178931
1104	86-87	0.29065380717367617
1104	88-89	0.297631826847482
1104	90-91	0.2651401513684206
1104	92-93	0.33581755997728635
1104	94-95	0.3647597659535222
1104	96-97	0.364805012690681
1104	98-99	0.37086530177236554
1104	100	0.3657138933029387
1105	1	-0.029850106293508816
1105	2	0.06268601469479052
1105	3	0.07181486649842128
1105	4	-0.10029690331806052
1105	5	-0.10525183500437407
1105	6	-0.15052638836045418
1105	7	-0.5008047684317916
1105	8	-0.4018755071088975
1105	9	-0.34019903854888867
1105	10-11	-0.4127431335231506
1105	12-13	-0.24959694405533384
1105	14-15	-0.22242063026292413
1105	16-17	-0.13508450724035725
1105	18-19	-0.16698988637985224
1105	20-21	-0.1737549734529651
1105	22-23	-0.1840241657838817
1105	24-25	-0.18674285225372245
1105	26-27	-0.18246551580656245
1105	28-29	-0.24182978722084414
1105	30-31	-0.21836732935823022
1105	32-33	-0.217534681944187
1105	34-35	-0.22167551387394013
1105	36-37	-0.2496474537991773
1105	38-39	-0.20491572753909537
1105	40-41	-0.23078861970350317
1105	42-43	-0.2759680839629368
1105	44-45	-0.2911350025105719
1105	46-47	-0.18957082931697755
1105	48-49	-0.1444029897821153
1105	50-51	-0.1724272178491688
1105	52-53	-0.16172838840193293
1105	54-55	-0.16052599815308355
1105	56-57	-0.15381357931917705
1105	58-59	-0.13452107567194105
1105	60-61	-0.09429783988122864
1105	62-63	-0.10319821303207277
1105	64-65	-0.07826354362612875
1105	66-67	-0.05745200901601777
1105	68-69	-0.05077918917278623
1105	70-71	-0.09408098437449297
1105	72-73	-0.03517395866562367
1105	74-75	-0.10737328664158241
1105	76-77	-0.052772528034395805
1105	78-79	-0.06886406885192997
1105	80-81	-0.08855990808148562
1105	82-83	-0.06544885401759615
1105	84-85	-0.07464706904831786
1105	86-87	-0.11515601678784648
1105	88-89	-0.05171876479249349
1105	90-91	-0.0940600853114617
1105	92-93	-0.09752592299443563
1105	94-95	-0.09681097805234984
1105	96-97	-0.10115286119383171
1105	98-99	-0.10063601172196357
1105	100	-0.04295912128395685
1106	1	0.08632141497716361
1106	2	0.05643750334053976
1106	3	0.06390245039047215
1106	4	0.0750178449593335
1106	5	0.08986253522034104
1106	6	0.07499211082561175
1106	7	0.08496797314831639
1106	8	0.06952926207569732
1106	9	0.043021594095286275
1106	10-11	0.09355014278132501
1106	12-13	0.08346864419834077
1106	14-15	0.13022328905205427
1106	16-17	0.16660056936402157
1106	18-19	0.14383090009809507
1106	20-21	0.1018467772788938
1106	22-23	0.13854615319595354
1106	24-25	0.1703744651376553
1106	26-27	0.20576597585731093
1106	28-29	0.17997907714023853
1106	30-31	0.20391733856185112
1106	32-33	0.20630099296204207
1106	34-35	0.26151876657312556
1106	36-37	0.2778458272351756
1106	38-39	0.28299829898089257
1106	40-41	0.3336073096703558
1106	42-43	0.3749334370881314
1106	44-45	0.3425184987560783
1106	46-47	0.3718064220925612
1106	48-49	0.41538609128637205
1106	50-51	0.42460968029451607
1106	52-53	0.32571293150529357
1106	54-55	0.3286988812490179
1106	56-57	0.2865074219742212
1106	58-59	0.33171291945065207
1106	60-61	0.2986778918849424
1106	62-63	0.3459153001831652
1106	64-65	0.30076889153648523
1106	66-67	0.3071064255733802
1106	68-69	0.2831017405642626
1106	70-71	0.3167630010462119
1106	72-73	0.24953028015283962
1106	74-75	0.29947563812682887
1106	76-77	0.23196718489558066
1106	78-79	0.19847052465699022
1106	80-81	0.28276985869593574
1106	82-83	0.2794006146871979
1106	84-85	0.3360949986869599
1106	86-87	0.29807318032039376
1106	88-89	0.3205538813453579
1106	90-91	0.3652812336385267
1106	92-93	0.33344173756674067
1106	94-95	0.329523087849541
1106	96-97	0.34028529307125766
1106	98-99	0.37101397929689384
1106	100	0.3789829117655259
1107	1	-0.042353110359499624
1107	2	0.008529603154215692
1107	3	0.05420295211579429
1107	4	-0.06512572367700642
1107	5	-0.06245380596768513
1107	6	-0.049465400672190185
1107	7	-0.009417340526731266
1107	8	0.01862877677483965
1107	9	0.05874460434846185
1107	10-11	0.05139367858802757
1107	12-13	0.07365730296343997
1107	14-15	0.05903052930126762
1107	16-17	0.09187838214695887
1107	18-19	0.09097257475230691
1107	20-21	0.08648685644958931
1107	22-23	0.10366578813312088
1107	24-25	0.0898550941259586
1107	26-27	0.053717969055462333
1107	28-29	-0.002245718685131237
1107	30-31	0.03985372566509682
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2216	36-37	-0.586374043311757
2216	38-39	-0.6983982236301856
2216	40-41	-0.7228001187187942
2216	42-43	-0.7173076145307036
2216	44-45	-0.6492456072648523
2216	46-47	-0.6740790748478247
2216	48-49	-0.6513594471798143
2216	50-51	-0.6185982417503837
2216	52-53	-0.5901557785339904
2216	54-55	-0.6710829064498611
2216	56-57	-0.6610473942327815
2216	58-59	-0.8046133695626594
2216	60-61	-0.7022790724056378
2216	62-63	-0.6993106261340216
2216	64-65	-0.6441230058251151
2216	66-67	-0.6522502837889874
2216	68-69	-0.7108483258055571
2216	70-71	-0.7473246974038048
2216	72-73	-0.7458850848824454
2216	74-75	-0.6877097824150624
2216	76-77	-0.6246285406843484
2216	78-79	-0.5783012418507596
2216	80-81	-0.6673667924446605
2216	82-83	-0.6013635501514152
2216	84-85	-0.6024542148781009
2216	86-87	-0.5771527872723539
2216	88-89	-0.5500335673229948
2216	90-91	-0.5288539891095709
2216	92-93	-0.7431094884400622
2216	94-95	-0.6729601371805778
2216	96-97	-0.7811912086853425
2216	98-99	-0.8122598140345509
2216	100	-0.8573147616727823
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	40.0
3	562.0
4	426.0
5	535.0
6	658.0
7	1258.0
8	2369.0
9	3975.0
10	4346.0
11	5124.0
12	7553.0
13	10762.0
14	11187.0
15	14538.0
16	19706.0
17	31880.0
18	37365.0
19	18386.0
20	10423.0
21	9426.0
22	9355.0
23	9494.0
24	10378.0
25	12689.0
26	15671.0
27	20023.0
28	24684.0
29	30630.0
30	38648.0
31	48628.0
32	60628.0
33	77287.0
34	110667.0
35	216715.0
36	359633.0
37	737374.0
38	924646.0
39	401439.0
40	4035.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.756151645727215	20.620214747479153	14.88460290124668	23.739030705546956
2	14.182228369906099	21.856469342362313	39.24279950507714	24.71850278265445
3	15.592951769641314	29.189734454654637	31.032533717292736	24.184780058411317
4	10.635503549749474	18.692783942114108	38.436172628328364	32.23553987980806
5	12.199532384762028	38.616251249189034	33.910732898938974	15.27348346710996
6	27.845025177535454	39.23762913080057	18.136907787522368	14.7804379041416
7	24.908064834008094	34.57670467188372	22.629598536908635	17.885631957199553
8	26.204072908741765	33.52900555622327	20.065313551366078	20.201607983668886
9	27.416282007772597	15.048576461872829	21.279490473164497	36.25565105719007
10-11	24.600948248380405	26.60555416462442	27.372520656841075	21.420976930154097
12-13	26.158949218971145	25.368126659972035	27.940146702701036	20.532777418355792
14-15	23.189171646519693	25.37321272497134	27.836972241286556	23.600643387222412
16-17	20.742834930246737	28.34061377300347	28.05623310889053	22.86031818785926
18-19	20.604951102631645	25.62506376502622	33.0111654263833	20.75881970595884
20-21	22.183508252594574	24.481001882146792	32.994999005492645	20.340490859765985
22-23	21.7614201510895	24.76793682620015	32.866378869933364	20.604264152776985
24-25	20.981780377592003	24.90169965445022	33.10305763421615	21.013462333741632
26-27	20.994197316663875	25.272052815407946	32.99963943257404	20.734110435354136
28-29	20.903501284317404	24.975383096539282	33.533800578938106	20.587315040205212
30-31	21.24652238878494	25.060840693731496	32.94989984388877	20.7427370735948
32-33	20.702461661519347	25.04581423082501	33.22813140608287	21.023592701572777
34-35	21.3599244923709	24.703301793608947	33.08657113165318	20.85020258236697
36-37	21.182194572159805	24.960941124753315	32.56348382309376	21.29338047999312
38-39	21.15991539382244	24.94223174602646	32.58739562924596	21.31045723090514
40-41	21.080384343199253	24.923347070688724	32.52357867568866	21.47268991042336
42-43	21.057285860143153	25.329176666659098	32.518211906745954	21.095325566451795
44-45	21.260648246539795	25.17279086538025	31.856262824972465	21.71029806310749
46-47	21.518625956070448	25.179558155316688	31.40879223781376	21.893023650799105
48-49	21.499459062939884	25.351563676700835	30.629420043114507	22.519557217244774
50-51	21.786286257496723	25.52326443866538	30.164345117177447	22.52610418666045
52-53	21.83225849812088	25.89971911592054	29.822120877637108	22.44590150832147
54-55	21.886927145096145	25.8128736800008	29.929896731188354	22.3703024437147
56-57	21.945579609087595	25.699744044907863	29.781235520312094	22.573440825692447
58-59	22.32683039066095	25.037585676572455	30.097195984402624	22.538387948363972
60-61	22.277170096670414	25.544964087855444	29.86919529821786	22.30867051725628
62-63	22.083527718902875	25.316024767925576	30.28841015965703	22.312037353514516
64-65	22.316472523290695	25.43023417393676	30.17362554391378	22.079667758858758
66-67	22.362120194488757	25.334672067012974	30.138552275476393	22.16465546302187
68-69	22.601632120356275	25.43176896192138	30.010718632786244	21.955880284936104
70-71	22.407751194491926	25.459435108707694	30.03945108168805	22.093362615112326
72-73	22.395974376506235	25.401583735236212	29.87797521782679	22.324466670430763
74-75	22.260097663437307	25.427975750500632	29.87523608657802	22.43669049948404
76-77	22.307360338127825	25.321456515707304	29.755554543047737	22.615628603117134
78-79	22.190102660825705	25.262313395557516	30.064197074877917	22.483386868738855
80-81	21.461987896893813	25.750268155643198	30.142995150946728	22.64474879651626
82-83	21.774217352993848	25.615183184652363	30.073432499727907	22.537166962625886
84-85	21.708243178316657	25.217696550550738	29.870649058598463	23.20341121253414
86-87	21.873008520693222	25.504709919515854	29.685026497554595	22.937255062236332
88-89	21.58460561057879	25.38774566130993	29.784434286645013	23.243214441466264
90-91	21.620314901868475	25.50959441795832	29.566050602579246	23.304040077593953
92-93	21.538534664304407	25.585125514221392	30.040414138197036	22.835925683277164
94-95	21.572692251975525	25.137563684368036	30.084982122132605	23.204761941523834
96-97	21.510168727982588	25.895637025940733	29.681420424889833	22.91277382118685
98-99	21.0614088945079	25.56208281260596	30.101039274953738	23.275469017932398
100	21.319105236930078	25.553504920615598	30.07448891052551	23.05290093192881
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	12396.0
1	7441.0
2	5956.0
3	8075.5
4	4195.0
5	2655.5
6	7134.5
7	20036.0
8	33070.0
9	31392.0
10	16847.5
11	5653.5
12	3459.5
13	3328.5
14	3495.0
15	3718.0
16	4100.5
17	4513.5
18	5043.5
19	5546.5
20	6272.0
21	7422.0
22	8615.0
23	10299.0
24	12809.0
25	15968.5
26	19550.0
27	23921.0
28	29303.0
29	35124.0
30	39498.5
31	43235.0
32	47717.0
33	52442.5
34	57327.0
35	62601.5
36	67693.0
37	72824.5
38	79523.5
39	83987.5
40	89249.5
41	94817.5
42	97883.5
43	104419.0
44	108573.0
45	108627.0
46	110826.5
47	112568.0
48	111361.5
49	114716.0
50	123248.5
51	120712.0
52	111825.0
53	106853.5
54	103130.0
55	99651.0
56	87501.0
57	74094.0
58	73161.0
59	74908.5
60	67635.0
61	55902.0
62	47734.5
63	37362.5
64	25922.5
65	20102.5
66	15856.5
67	11797.5
68	8542.0
69	6114.0
70	4787.5
71	4776.0
72	3765.5
73	1933.5
74	1399.5
75	1184.5
76	861.0
77	595.5
78	375.0
79	179.5
80	73.0
81	30.5
82	18.0
83	10.5
84	5.5
85	4.5
86	3.5
87	3.5
88	3.5
89	3.0
90	2.5
91	1.0
92	0.5
93	1.5
94	4.5
95	7.0
96	9.0
97	8.5
98	4.5
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008688694373813062
2	6.054839284887151E-5
3	4.843871427909721E-4
4	0.0028155002674725253
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	4.541129463665364E-5
24-25	3.0274196424435756E-5
26-27	4.843871427909721E-4
28-29	8.022662052475476E-4
30-31	0.00149857272300957
32-33	0.003935645535176648
34-35	0.005010379508244118
36-37	0.0035269438834467657
38-39	0.0022554276336204638
40-41	0.003193927722777972
42-43	4.6925004457875426E-4
44-45	5.146613392154078E-4
46-47	0.0031636535263535365
48-49	0.002376524419318207
50-51	9.9904848200638E-4
52-53	3.0274196424435756E-5
54-55	4.843871427909721E-4
56-57	7.41717812398676E-4
58-59	6.963065177620224E-4
60-61	3.7842745530544696E-4
62-63	0.0
64-65	0.0
66-67	6.508952231253688E-4
68-69	4.9952424100319E-4
70-71	0.001967822767588324
72-73	7.568549106108939E-5
74-75	0.0019526856693761062
76-77	4.087016517298827E-4
78-79	9.9904848200638E-4
80-81	7.265807141864582E-4
82-83	0.0012563791516140839
84-85	0.001089871071279687
86-87	2.4219357139548605E-4
88-89	0.0013169275444629553
90-91	0.0015288469194340059
92-93	0.0027549518746236537
94-95	0.001105008169491905
96-97	0.002270564731832682
98-99	0.002149467946134939
100	0.0030576938388680117
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3303143.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.01208725842822
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.18893753330558	22.47388681941893
2	16.23578686863174	11.368975731075551
3	6.92518107855322	7.273951326081642
4	3.7481928803493534	5.249282247528439
5	2.2649069802687096	3.9649560412695615
6	1.4571773601954237	3.0611292531700953
7	0.9921879723470688	2.4317000305204974
8	0.7511275703892539	2.103883522934379
9	0.5535369717777592	1.7442436280984117
>10	2.5576045642832135	15.520275694788838
>50	0.17043693814632047	4.167689253466035
>100	0.13361955786670546	9.306321370072629
>500	0.01472208561228499	3.4071307535889552
>1k	0.005975434748334602	3.4282642422966263
>5k	1.732010071981044E-4	0.4599641677557538
>10k+	4.33002517995261E-4	4.038345917933642
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	46871	1.4189818606097284	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31635	0.9577242038870252	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29405	0.8902127458605333	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	15261	0.4620145116333141	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10016	0.30322635138714854	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9160	0.27731163924783153	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6010	0.1819479205108589	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4875	0.14758670756912431	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4077	0.12342789882242458	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.0274196424435756E-5	0.0
3	0.0	0.0	0.0	6.054839284887151E-5	0.0
4	0.0	0.0	0.0	6.054839284887151E-5	0.0
5	0.0	0.0	0.0	6.054839284887151E-5	0.0
6	0.0	0.0	0.0	6.054839284887151E-5	0.0
7	0.0	0.0	0.0	6.054839284887151E-5	0.0
8	0.0	0.0	0.0	6.054839284887151E-5	0.0
9	0.0	0.0	0.0	6.054839284887151E-5	0.0
10-11	0.0	0.0	0.0	7.568549106108939E-5	0.0
12-13	0.0	0.0	0.0	1.9678227675883243E-4	0.0
14-15	0.0	0.0	0.0	2.119193749710503E-4	0.0
16-17	0.0	0.0	0.0	2.2705647318326818E-4	0.0
18-19	0.0	0.0	0.0	4.843871427909721E-4	0.0
20-21	0.0	0.0	0.0	6.508952231253687E-4	0.0
22-23	0.0	0.0	0.0	0.001241242053401866	0.0
24-25	0.0	0.0	0.0	0.00199809696401276	0.0
26-27	0.0	0.0	0.0	0.0028155002674725253	0.0
28-29	0.0	0.0	0.0	0.005161750490366296	0.0
30-31	0.0	0.0	0.0	0.010868436516372437	0.0
32-33	0.0	0.0	0.0	0.019723638970519895	0.0
34-35	0.0	0.0	0.0	0.03159112396889871	0.0
36-37	0.0	0.0	0.0	0.049483174055740244	0.0
38-39	0.0	0.0	0.0	0.07445938610589975	0.0
40-41	0.0	0.0	0.0	0.10116122735225208	0.0
42-43	0.0	0.0	0.0	0.12532003609895181	0.0
44-45	0.0	0.0	0.0	0.15509470828238436	0.0
46-47	0.0	0.0	0.0	0.19118155042031182	0.0
48-49	0.0	0.0	0.0	0.23180952202190458	0.0
50-51	0.0	0.0	0.0	0.275328679382031	0.0
52-53	0.0	0.0	0.0	0.31915057870640173	0.0
54-55	0.0	0.0	0.0	0.3645315991466309	0.0
56-57	0.0	0.0	0.0	0.42094756418356694	0.0
58-59	0.0	0.0	0.0	0.4850229009158853	0.0
60-61	0.0	0.0	0.0	0.5569998029149813	0.0
62-63	0.0	0.0	0.0	0.6307931566995435	0.0
64-65	0.0	0.0	0.0	0.7038145184752824	0.0
66-67	0.0	0.0	0.0	0.7869020505621465	0.0
68-69	0.0	0.0	0.0	0.8882903343875818	0.0
70-71	0.0	0.0	0.0	1.0056785310233314	0.0
72-73	0.0	0.0	0.0	1.132103575291775	0.0
74-75	0.0	0.0	0.0	1.2606629504081415	0.0
76-77	0.0	0.0	0.0	1.3962156648985529	0.0
78-79	0.0	0.0	0.0	1.5526273007254	0.0
80-81	0.0	0.0	0.0	1.7311996483349343	0.0
82-83	0.0	0.0	0.0	1.9285419977276188	0.0
84-85	0.0	0.0	0.0	2.1276251134147084	0.0
86-87	0.0	0.0	0.0	2.3388481818679967	0.0
88	0.0	0.0	0.0	2.5081566253716536	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10500	0.0	62.630085	1
GTATCAA	22640	0.0	49.539154	1
ATCAACG	31665	0.0	35.355556	3
TCAACGC	31975	0.0	35.012783	4
TATCAAC	33230	0.0	33.718742	2
CAACGCA	34055	0.0	32.85899	5
AACGCAG	34945	0.0	32.075912	6
ACGCAGA	37575	0.0	29.74326	7
CGCAGAG	37890	0.0	29.458775	8
GCAGAGT	39830	0.0	27.846933	9
GTGGTAT	3495	0.0	26.899078	1
TGGTATC	3650	0.0	25.495747	2
GTACATG	19735	0.0	25.271606	1
TACATGG	19375	0.0	24.718828	2
TAACGCA	295	0.0	23.898172	4
AGAGTAC	39185	0.0	23.501799	10-11
AGTACTT	29775	0.0	23.36858	12-13
ACATGGG	20350	0.0	23.00331	3
TATAACG	315	0.0	22.380825	2
GAGTACT	29565	0.0	22.3026	12-13
>>END_MODULE