FastQCFastQC Report
Wed 25 May 2016
SRR2431374_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431374_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3290898
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA495001.5041487156393178No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG3500.038.942692
GGTATCA40700.037.7615131
GTATCAA74350.036.3481981
TACCTGG33150.035.8701362
GTACCTG34900.034.47551
ATAACGC3750.033.839863
TCAACGC80950.031.2944954
ATCAACG81850.031.0652333
CAACGCA84250.030.1245025
AACGCAG88400.028.7634536
TATCAAC92600.028.3216972
ACCTGGG40000.028.199883
GTCTCGC7300.026.397151
TAACGCA5500.025.6362554
CGCAGAG97850.025.6013228
ACGCAGA99500.025.3184857
GTACATG119650.025.1791671
TACATGG115350.024.284252
TCTGTCG7150.022.3495568
ACATGGG122850.021.8069923
Produced by FastQC (version 0.11.5)