##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431374_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3290898
Sequences flagged as poor quality	0
Sequence length	100
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.716486199207633	33.0	31.0	34.0	26.0	34.0
2	30.802206570972423	33.0	31.0	34.0	26.0	34.0
3	30.92378827906547	34.0	31.0	34.0	26.0	34.0
4	34.073118644212	37.0	35.0	37.0	30.0	37.0
5	34.258315511450064	37.0	35.0	37.0	31.0	37.0
6	34.27326918063094	37.0	35.0	37.0	30.0	37.0
7	34.18699455285457	37.0	35.0	37.0	30.0	37.0
8	34.13099464036868	37.0	35.0	37.0	30.0	37.0
9	35.539183225976615	39.0	37.0	39.0	29.0	39.0
10-11	35.573616988432946	39.0	37.0	39.0	30.0	39.0
12-13	35.53658894927767	39.0	37.0	39.0	29.5	39.0
14-15	36.88395978848327	41.0	38.0	41.0	29.5	41.0
16-17	36.88406325568279	40.5	38.0	41.0	30.0	41.0
18-19	36.8703668421203	40.5	38.0	41.0	29.5	41.0
20-21	36.91625598848703	41.0	38.0	41.0	29.0	41.0
22-23	36.928842066815804	40.0	38.0	41.0	29.0	41.0
24-25	36.973433391129106	40.5	38.0	41.0	29.0	41.0
26-27	36.99398872283492	40.0	38.0	41.0	30.0	41.0
28-29	36.970662263005416	40.0	38.0	41.0	30.0	41.0
30-31	36.908357232585146	40.0	38.0	41.0	30.0	41.0
32-33	36.82782040038919	40.0	38.0	41.0	30.0	41.0
34-35	36.760410684256996	40.0	38.0	41.0	29.5	41.0
36-37	36.66170160849713	40.0	37.0	41.0	29.0	41.0
38-39	36.55201908415272	40.0	37.0	41.0	28.5	41.0
40-41	36.46641008016657	40.0	37.0	41.0	28.0	41.0
42-43	36.349583609093926	40.0	37.0	41.0	28.0	41.0
44-45	36.22878132351717	40.0	37.0	41.0	27.5	41.0
46-47	36.10630502677385	40.0	36.0	41.0	27.0	41.0
48-49	35.98935609672497	40.0	36.0	41.0	27.0	41.0
50-51	35.87273488877504	40.0	36.0	41.0	27.0	41.0
52-53	35.72193911813736	40.0	35.0	41.0	27.0	41.0
54-55	35.57540874861512	40.0	35.0	41.0	27.0	41.0
56-57	35.40960582795334	39.0	35.0	41.0	27.0	41.0
58-59	35.2079803141878	39.0	35.0	41.0	26.5	41.0
60-61	35.04511306640315	39.0	35.0	41.0	26.0	41.0
62-63	34.891707825645156	39.0	34.5	41.0	26.0	41.0
64-65	34.64947926067596	38.0	34.0	40.5	26.0	41.0
66-67	34.37116358513694	38.0	34.0	40.0	26.0	41.0
68-69	34.07080392646627	37.0	34.0	40.0	25.5	41.0
70-71	33.82357672586632	37.0	34.0	40.0	25.0	41.0
72-73	33.585826269911735	36.5	34.0	39.0	25.0	41.0
74-75	33.25021559464924	36.0	33.5	39.0	24.5	41.0
76-77	32.89547822509236	35.5	33.0	39.0	24.0	40.5
78-79	32.54956018083818	35.0	33.0	37.5	23.0	39.5
80-81	32.19621954250785	35.0	33.0	37.0	22.0	39.0
82-83	31.850289495450788	35.0	32.5	37.0	21.0	39.0
84-85	31.53474598726548	35.0	32.0	36.0	20.0	38.0
86-87	31.25032559501996	35.0	32.0	36.0	19.5	37.0
88-89	30.98046657781554	35.0	32.0	35.5	18.0	37.0
90-91	30.7518441774859	35.0	32.0	35.0	17.0	36.5
92-93	30.522498266430624	35.0	32.0	35.0	9.0	36.0
94-95	30.297298336198814	35.0	31.0	35.0	4.0	36.0
96-97	30.094884739666803	35.0	31.0	35.0	2.0	36.0
98-99	29.9581500247045	35.0	31.0	35.0	2.0	35.5
100	29.9033664975335	35.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.602121044784905
1101	2	0.5807422785954799
1101	3	0.5570187293383455
1101	4	0.5821369717550837
1101	5	0.46517503555144657
1101	6	0.5154791990449112
1101	7	0.39655301798834586
1101	8	0.4384242629223962
1101	9	0.6192716491113615
1101	10-11	0.4902214230744306
1101	12-13	0.5314584741126183
1101	14-15	0.5733220143071591
1101	16-17	0.5748751223078088
1101	18-19	0.6481022291638254
1101	20-21	0.5439373678978612
1101	22-23	0.5443828436378908
1101	24-25	0.5939487087978108
1101	26-27	0.6539055947661154
1101	28-29	0.6784279487434972
1101	30-31	0.6847937165590139
1101	32-33	0.6606335461905886
1101	34-35	0.7152505611686806
1101	36-37	0.773835064284313
1101	38-39	0.716270236273445
1101	40-41	0.6767211923294383
1101	42-43	0.7073458805380852
1101	44-45	0.6753418403401383
1101	46-47	0.616225192995465
1101	48-49	0.6062542604381136
1101	50-51	0.6070645494355205
1101	52-53	0.5779227495553982
1101	54-55	0.6288134742824951
1101	56-57	0.6529914819713625
1101	58-59	0.6173182756771567
1101	60-61	0.6750131993215547
1101	62-63	0.6923821009628952
1101	64-65	0.6676777867385439
1101	66-67	0.6101475277130319
1101	68-69	0.6453857536571164
1101	70-71	0.6733184495050892
1101	72-73	0.6881381469162875
1101	74-75	0.6743054559025694
1101	76-77	0.6621575469567773
1101	78-79	0.6100434534839465
1101	80-81	0.6035167269203114
1101	82-83	0.5749591886491423
1101	84-85	0.5908831113802719
1101	86-87	0.6664823594548182
1101	88-89	0.6727190583261482
1101	90-91	0.6398232804696278
1101	92-93	0.589272965431384
1101	94-95	0.6432263877428319
1101	96-97	0.70399014004321
1101	98-99	0.7305982296970193
1101	100	0.7450026697792289
1102	1	0.25710820878130747
1102	2	0.37012012754532364
1102	3	0.3783836606843174
1102	4	0.3232123368846942
1102	5	0.19717415466607235
1102	6	0.2593920488654291
1102	7	0.2522540895183525
1102	8	0.24129615889261657
1102	9	0.28571560937609064
1102	10-11	0.25339164441359685
1102	12-13	0.3039529728714001
1102	14-15	0.3912270894196297
1102	16-17	0.42809188110688723
1102	18-19	0.4177298065871753
1102	20-21	0.4493955407980863
1102	22-23	0.3774231660123064
1102	24-25	0.46864816359410355
1102	26-27	0.5076679819907852
1102	28-29	0.526051858040141
1102	30-31	0.5226642918337276
1102	32-33	0.4742771982358036
1102	34-35	0.5046355038302579
1102	36-37	0.553357895147002
1102	38-39	0.5482583398026009
1102	40-41	0.589177469988698
1102	42-43	0.5471399812628306
1102	44-45	0.5715117465739183
1102	46-47	0.5603306529033887
1102	48-49	0.4786455931332796
1102	50-51	0.43093236367497667
1102	52-53	0.46576242426036885
1102	54-55	0.42139047426415743
1102	56-57	0.414001125895048
1102	58-59	0.46528758547888316
1102	60-61	0.49449845519987434
1102	62-63	0.4764815054616136
1102	64-65	0.4775488944482973
1102	66-67	0.5299469039905205
1102	68-69	0.49385867180861
1102	70-71	0.4696548230135065
1102	72-73	0.5193645123179849
1102	74-75	0.5097858795625285
1102	76-77	0.4958990398116967
1102	78-79	0.44244900161595524
1102	80-81	0.43138067296327876
1102	82-83	0.4360262690463763
1102	84-85	0.3757279203963151
1102	86-87	0.37624440925376135
1102	88-89	0.4478524630303511
1102	90-91	0.43625898853365896
1102	92-93	0.4082454182444195
1102	94-95	0.44018994477026396
1102	96-97	0.447198046612975
1102	98-99	0.3729523967646813
1102	100	0.378665729677536
1103	1	0.07780974259918594
1103	2	0.0688594881049589
1103	3	0.13775704465150795
1103	4	0.09982896807868258
1103	5	0.05320055162702886
1103	6	0.005084780127006638
1103	7	0.050858215098550374
1103	8	0.013710238699516708
1103	9	0.0362565177998988
1103	10-11	0.0774715332021998
1103	12-13	0.05700599543932583
1103	14-15	0.0953084521246268
1103	16-17	0.08611004933113264
1103	18-19	0.03561666397693841
1103	20-21	0.013346978401976628
1103	22-23	0.050620153679901136
1103	24-25	0.07084867669337314
1103	26-27	0.07331395104988303
1103	28-29	0.10528132099917542
1103	30-31	0.1454218445530202
1103	32-33	0.13569639706775405
1103	34-35	0.14237661356332865
1103	36-37	0.16171358392892898
1103	38-39	0.16356568601611343
1103	40-41	0.14392748053916904
1103	42-43	0.06631317714820284
1103	44-45	0.054701226086166344
1103	46-47	0.08493938793667866
1103	48-49	0.11694509042700219
1103	50-51	0.01181273898196622
1103	52-53	0.011316204257560969
1103	54-55	0.025511960249033905
1103	56-57	-0.03917226651251582
1103	58-59	0.004716266506370914
1103	60-61	-0.008907614438271594
1103	62-63	-0.00865083522471366
1103	64-65	0.03303885512496407
1103	66-67	0.04581937746789322
1103	68-69	0.0887632987093454
1103	70-71	0.04016739318987561
1103	72-73	0.04691383655251258
1103	74-75	0.07811843919473205
1103	76-77	0.11406225652716984
1103	78-79	0.13210708450422715
1103	80-81	0.09920983345450907
1103	82-83	0.0853977759747302
1103	84-85	0.12527802945982813
1103	86-87	0.10619410713239219
1103	88-89	0.03704503398777703
1103	90-91	0.017218728570078667
1103	92-93	0.05932358179497044
1103	94-95	0.06687242979332098
1103	96-97	0.09652199769154635
1103	98-99	0.07546583365429171
1103	100	-0.003102239347541058
1104	1	0.2477114049283351
1104	2	0.36823503568247773
1104	3	0.31163743537416977
1104	4	0.21915788223355293
1104	5	0.2524470395299119
1104	6	0.2738232453967484
1104	7	0.3163163699540874
1104	8	0.2888228819695584
1104	9	0.34614232113084853
1104	10-11	0.274583407985304
1104	12-13	0.2566966611013797
1104	14-15	0.36274627335409093
1104	16-17	0.45699572615354356
1104	18-19	0.3413641616765517
1104	20-21	0.363258055482369
1104	22-23	0.3719679099182258
1104	24-25	0.3695480850737596
1104	26-27	0.4442535774722671
1104	28-29	0.45392989007230966
1104	30-31	0.45865501306413137
1104	32-33	0.49038793291283156
1104	34-35	0.5040060242816793
1104	36-37	0.5066119787033543
1104	38-39	0.48071118341307795
1104	40-41	0.47811244580348955
1104	42-43	0.43187789447677005
1104	44-45	0.46113025357450965
1104	46-47	0.4479812734450519
1104	48-49	0.4556530416282598
1104	50-51	0.47370409575804473
1104	52-53	0.45375281107497756
1104	54-55	0.40714212582494724
1104	56-57	0.4515508485889299
1104	58-59	0.46142929167842084
1104	60-61	0.42576841886912575
1104	62-63	0.43103726881422233
1104	64-65	0.5233777267596835
1104	66-67	0.5564889046719514
1104	68-69	0.5825014756654312
1104	70-71	0.5547196316046481
1104	72-73	0.5364519388615676
1104	74-75	0.5824485607722281
1104	76-77	0.552978946812253
1104	78-79	0.4763750334269261
1104	80-81	0.4892311931277149
1104	82-83	0.5056618398272938
1104	84-85	0.5656706672222143
1104	86-87	0.5491415096360726
1104	88-89	0.5424022074236419
1104	90-91	0.5575784898393863
1104	92-93	0.5730798124277179
1104	94-95	0.5629229259199597
1104	96-97	0.5308002892112427
1104	98-99	0.5242930436133832
1104	100	0.5101708474991682
1105	1	0.307478105594182
1105	2	0.3222810380008134
1105	3	0.37334119074780503
1105	4	0.36346903058773705
1105	5	0.30508972552742364
1105	6	0.32788330783566266
1105	7	0.28486006565501754
1105	8	0.310023050691008
1105	9	0.35222358757066274
1105	10-11	0.3808521735331496
1105	12-13	0.42435619898151344
1105	14-15	0.39813064586482483
1105	16-17	0.3812427080288572
1105	18-19	0.38536372771568494
1105	20-21	0.43255335096250036
1105	22-23	0.40515838385176295
1105	24-25	0.45821172523193354
1105	26-27	0.48764032575576266
1105	28-29	0.5142743903363325
1105	30-31	0.5362830663171039
1105	32-33	0.5718583369756729
1105	34-35	0.6066383193567475
1105	36-37	0.5459048841391763
1105	38-39	0.569198653607998
1105	40-41	0.4673890050276981
1105	42-43	0.4822545533022975
1105	44-45	0.49448018125651316
1105	46-47	0.5113632795212126
1105	48-49	0.5104929077787332
1105	50-51	0.494835043052511
1105	52-53	0.46463191747149324
1105	54-55	0.46032292417550735
1105	56-57	0.4742186965641437
1105	58-59	0.49729123257059626
1105	60-61	0.531387740751974
1105	62-63	0.5483021102141237
1105	64-65	0.5627120152885112
1105	66-67	0.5250813174573565
1105	68-69	0.41849699651273653
1105	70-71	0.48260918460028535
1105	72-73	0.4694977283399808
1105	74-75	0.4949604735529718
1105	76-77	0.449252631640384
1105	78-79	0.4834584138182052
1105	80-81	0.44909888704064826
1105	82-83	0.4417658156523423
1105	84-85	0.4770903218260045
1105	86-87	0.5049078725418781
1105	88-89	0.5660572811273923
1105	90-91	0.5414103422497014
1105	92-93	0.44910650516140294
1105	94-95	0.4543561323304566
1105	96-97	0.4850775435128405
1105	98-99	0.573447965175351
1105	100	0.49962566896646976
1106	1	0.17950341034303463
1106	2	0.22569472323115747
1106	3	0.21761812811857695
1106	4	0.08333317816049401
1106	5	0.05139849395695961
1106	6	0.12872899210332633
1106	7	0.10377990934797054
1106	8	0.17533113847401438
1106	9	0.1738327591700184
1106	10-11	0.15691297331453313
1106	12-13	0.14670633653207688
1106	14-15	0.19138110863319469
1106	16-17	0.18596481722867253
1106	18-19	0.20092336554849055
1106	20-21	0.19151078708522817
1106	22-23	0.20155385709770712
1106	24-25	0.19376957154474894
1106	26-27	0.25413254927291007
1106	28-29	0.26874022698395095
1106	30-31	0.300801996506884
1106	32-33	0.3127051811849526
1106	34-35	0.269428137854554
1106	36-37	0.3245790749713606
1106	38-39	0.2767988351683499
1106	40-41	0.2952073866781433
1106	42-43	0.29287680054008547
1106	44-45	0.3220948358736564
1106	46-47	0.32429971676874203
1106	48-49	0.31158632887005666
1106	50-51	0.32020194216896414
1106	52-53	0.27618037726809064
1106	54-55	0.2602258691738797
1106	56-57	0.27299096471937645
1106	58-59	0.25622911106196256
1106	60-61	0.28986594322911685
1106	62-63	0.2947569157842338
1106	64-65	0.3828198980501938
1106	66-67	0.35449296001086594
1106	68-69	0.3181984589975926
1106	70-71	0.3070145445463126
1106	72-73	0.26370278783164025
1106	74-75	0.2793133199276312
1106	76-77	0.32059888723662766
1106	78-79	0.33350100235242763
1106	80-81	0.28398472085230964
1106	82-83	0.2641084086674148
1106	84-85	0.3128370805223568
1106	86-87	0.33676995319042646
1106	88-89	0.31187942292189774
1106	90-91	0.2863056161511537
1106	92-93	0.3543494330604666
1106	94-95	0.28356994585886497
1106	96-97	0.2436792764951008
1106	98-99	0.21185910402257235
1106	100	0.1927732729293119
1107	1	0.19213717037811406
1107	2	0.27023038879467265
1107	3	0.2399858660223657
1107	4	0.1718002664859526
1107	5	0.16429193804469122
1107	6	0.23445536923555466
1107	7	0.23770446616185126
1107	8	0.21893949639689936
1107	9	0.16170124064788638
1107	10-11	0.3212431554958357
1107	12-13	0.32573811973988853
1107	14-15	0.4740229427374061
1107	16-17	0.4147416774161101
1107	18-19	0.39525217045721206
1107	20-21	0.4029593307889954
1107	22-23	0.39229557415924887
1107	24-25	0.4372990334030078
1107	26-27	0.44184701901239265
1107	28-29	0.41938632385250685
1107	30-31	0.3650212313251515
1107	32-33	0.4013098874033787
1107	34-35	0.4267925032997226
1107	36-37	0.49587775333650796
1107	38-39	0.4354686567054671
1107	40-41	0.4781298920193535
1107	42-43	0.5016411755948127
1107	44-45	0.4651128914141225
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2216	98-99	-2.419274124170869
2216	100	-2.409060042907832
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	73638.0
3	17152.0
4	12809.0
5	13188.0
6	8208.0
7	6421.0
8	6971.0
9	7037.0
10	7003.0
11	7439.0
12	8587.0
13	9386.0
14	9648.0
15	9597.0
16	9393.0
17	9360.0
18	9183.0
19	8702.0
20	8428.0
21	9047.0
22	11511.0
23	21290.0
24	37785.0
25	43684.0
26	38593.0
27	27530.0
28	27636.0
29	30870.0
30	36678.0
31	44785.0
32	58439.0
33	77221.0
34	108898.0
35	179219.0
36	291258.0
37	584142.0
38	947768.0
39	470307.0
40	2087.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.916624378207715	22.808632315270337	13.403577755561566	21.871165550960377
2	14.6613959610975	24.16780971979043	40.08880261716117	21.081991701950898
3	16.0402869128307	31.15337924789457	30.78284174973677	22.023492089537953
4	11.092453572660325	21.40423805682041	40.181500777144215	27.321807593375052
5	10.84586411903145	41.35722956824815	34.57898231332206	13.21792399939834
6	25.29638575743938	43.28783307408284	19.28419516016454	12.131586008313235
7	22.801800602753413	36.997530765158935	24.2854381995431	15.915230432544552
8	22.083516414060846	39.31589493202159	21.943736937455977	16.656851716461585
9	23.686756623875915	20.70930183797857	21.836532156268593	33.767409381876924
10-11	20.79496234766316	31.129816238607212	29.643246311493094	18.431975102236535
12-13	22.061425179388728	29.53462854211829	28.9371168598966	19.466829418596383
14-15	20.108447603055456	30.688660055705157	26.6935043261748	22.509388015064584
16-17	19.37322274953523	33.437408269718475	26.856514544054537	20.332854436691747
18-19	19.341590046242697	32.00503327663149	28.988409850442036	19.66496682668378
20-21	21.34690895919594	31.286247705033706	27.983334639967573	19.38350869580279
22-23	20.524337126219045	31.591377186409304	28.189646108752083	19.694639578619576
24-25	20.32990691294595	31.234468525004424	28.32527778132291	20.110346780726722
26-27	20.19862055888697	31.09569181420998	28.355224014843365	20.350463612059688
28-29	20.305901307181202	31.0242523469278	28.467321077711922	20.20252526817908
30-31	20.705275581315494	31.61053609075699	27.810904500838372	19.873283827089143
32-33	19.819043312797906	31.184892391073802	28.71143377886522	20.284630517263068
34-35	20.130067841665102	30.611720569887	28.67563807811728	20.582573510330615
36-37	20.81942375606901	31.22454722084975	27.904237688314858	20.051791334766378
38-39	20.452153181289727	31.13042397546202	28.316602337720585	20.10082050552767
40-41	20.159163243588832	30.85466337759481	28.5352052843935	20.45096809442286
42-43	19.923877920251552	31.005989854440948	28.563449854720506	20.506682370586997
44-45	20.38669992202736	30.943013122861906	28.42067727410573	20.249609681005
46-47	20.254942571905904	31.519178048058617	28.140844839311335	20.08503454072414
48-49	20.103737642430726	30.90814422081754	28.631744283779074	20.356373852972652
50-51	20.256082078508662	30.641013486288543	28.421041916218613	20.68186251898418
52-53	20.083697519643575	30.926923289630974	28.396489347284543	20.592889843440908
54-55	20.361418068867525	31.362427519783353	28.145235738087294	20.130918673261828
56-57	20.051684980816788	31.1180869173095	28.124876553451365	20.705351548422346
58-59	19.860582126823743	30.565790857085208	28.655673922436975	20.917953093654074
60-61	20.592570781592137	31.086332666646005	27.916833034630667	20.40426351713119
62-63	20.203619194517728	31.00503266889463	28.198215198404814	20.59313293818283
64-65	20.168069652731514	30.655299959965326	28.515063140070453	20.661567247232707
66-67	20.11517828872241	30.773728021956316	28.126061640318234	20.98503204900304
68-69	20.62821454812638	30.982227343418117	28.004924491734474	20.38463361672103
70-71	20.739096137285323	30.63338638876076	28.1630120410903	20.464505432863614
72-73	20.272308652531922	30.73885911991195	28.614028754461547	20.374803473094577
74-75	20.21200596311402	30.60761834611708	28.501202407367227	20.67917328340167
76-77	20.38943473787398	30.87081398451122	28.12025775335486	20.619493524259944
78-79	20.466450190798984	30.977988378855866	28.257302414113106	20.29825901623204
80-81	20.380349071894663	30.704126958659916	28.31737720221046	20.59814676723496
82-83	20.05650743285754	30.8124280495774	28.55117354871145	20.579890968853608
84-85	20.291995378735255	30.635873631138722	28.287303008258597	20.784827981867434
86-87	20.48142192812781	30.83883506887495	28.184494882865565	20.495248120131677
88-89	20.111031224759067	30.660516970717634	28.431505421501125	20.79694638302217
90-91	20.079776747872142	30.606855427777095	28.320107353557646	20.99326047079312
92-93	20.22039758717076	30.886657466340893	28.206965091281806	20.685979855206543
94-95	20.135434103084467	30.56050829799392	28.441541491640926	20.862516107280683
96-97	19.992229932021782	30.795541615824686	28.375151766262256	20.83707668589128
98-99	19.874131407649113	30.691503892807038	28.447256042807663	20.987108656736194
100	20.113699073049894	30.866794376551297	28.16865970258831	20.850846847810498
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	42811.0
1	26831.0
2	8603.0
3	4992.5
4	2864.0
5	1956.0
6	1841.5
7	2188.0
8	2856.5
9	2894.0
10	2057.0
11	1440.0
12	1376.0
13	1454.0
14	1535.0
15	1708.5
16	2021.0
17	2403.0
18	3048.5
19	4000.0
20	5465.5
21	7410.0
22	10203.5
23	14697.5
24	20364.5
25	26902.5
26	35418.0
27	46084.5
28	58113.5
29	69822.5
30	79201.5
31	87517.5
32	95577.5
33	102542.5
34	108415.0
35	113113.0
36	116593.0
37	119145.5
38	120866.5
39	121029.0
40	120145.5
41	117563.0
42	113140.5
43	110190.0
44	106492.0
45	100807.5
46	96955.0
47	93825.0
48	90229.5
49	87706.0
50	86190.0
51	81633.5
52	73936.0
53	68030.0
54	63666.0
55	60098.0
56	55645.5
57	49789.5
58	45170.5
59	41088.5
60	36104.0
61	30361.0
62	24786.5
63	19630.5
64	15181.0
65	11589.0
66	8781.0
67	6441.5
68	4613.0
69	3545.5
70	2695.5
71	1999.5
72	1518.5
73	1119.5
74	860.0
75	679.5
76	518.5
77	410.5
78	328.5
79	241.0
80	170.5
81	139.0
82	115.5
83	100.5
84	91.0
85	78.0
86	73.5
87	66.5
88	56.0
89	53.0
90	42.5
91	35.5
92	33.0
93	22.0
94	18.5
95	20.5
96	19.5
97	12.0
98	10.5
99	15.5
100	35.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.430947419215059E-4
2	1.2154737096075295E-4
3	9.116052822056472E-5
4	9.116052822056472E-5
5	9.116052822056472E-5
6	3.038684274018824E-5
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	1.519342137009412E-5
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	1.0635394959065883E-4
84-85	7.292842257645177E-4
86-87	0.0012914408164580002
88-89	0.0034033263869010827
90-91	0.0027196224252468474
92-93	0.0022030460986636473
94-95	0.0022030460986636473
96-97	0.0019599513567421416
98-99	0.0025828816329160004
100	0.0032210053304599535
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3290898.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.14751645287583
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.63329600359549	61.90715411884112
2	10.813246017066753	15.819241818847688
3	2.335542745556005	5.125174542208581
4	0.8168882908900126	2.3901339879215526
5	0.39978100264394734	1.4621493734222666
6	0.22430168596202876	0.9844266758589172
7	0.14711150484411428	0.7532588854694522
8	0.10844486587617487	0.6345978088725932
9	0.07566202727227679	0.4981040446281126
>10	0.40629967166547853	5.353330836075303
>50	0.025294319066066526	1.2685930752219097
>100	0.013468069711921153	1.7673766948143461
>500	3.318979255888171E-4	0.16854660588419637
>1k	2.904106835804341E-4	0.3657406710239483
>5k	0.0	0.0
>10k+	4.148724051149059E-5	1.502170860909844
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	49500	1.5041487156393178	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	3.0386842740188242E-5	0.0	0.0	3.0386842740188242E-5	0.0
10-11	3.0386842740188242E-5	0.0	0.0	6.0773685480376484E-5	0.0
12-13	3.0386842740188242E-5	0.0	0.0	1.367407923308471E-4	0.0
14-15	3.0386842740188242E-5	0.0	0.0	1.671276350710353E-4	0.0
16-17	3.0386842740188242E-5	0.0	0.0	1.9751447781122356E-4	0.0
18-19	3.0386842740188242E-5	0.0	0.0	2.2790132055141183E-4	0.0
20-21	3.0386842740188242E-5	0.0	0.0	2.886750060317883E-4	0.0
22-23	3.0386842740188242E-5	0.0	0.0	8.508315967252708E-4	0.0
24-25	3.0386842740188242E-5	0.0	0.0	0.0019295645140019533	0.0
26-27	3.0386842740188242E-5	0.0	0.0	0.0028563632175776944	0.0
28-29	3.0386842740188242E-5	0.0	0.0	0.005044215894871248	0.0
30-31	3.0386842740188242E-5	0.0	0.0	0.01054423443084532	0.0
32-33	3.0386842740188242E-5	0.0	0.0	0.02013128331537471	0.0
34-35	3.0386842740188242E-5	0.0	0.0	0.033699008598868756	0.0
36-37	3.0386842740188242E-5	1.5193421370094121E-5	0.0	0.05247807741230509	0.0
38-39	3.0386842740188242E-5	3.0386842740188242E-5	0.0	0.08207486224124844	0.0
40-41	3.0386842740188242E-5	3.0386842740188242E-5	0.0	0.11733879324123689	0.0
42-43	3.0386842740188242E-5	3.0386842740188242E-5	0.0	0.15248117687026458	0.0
44-45	3.0386842740188242E-5	3.0386842740188242E-5	0.0	0.1892036763217821	0.0
46-47	3.0386842740188242E-5	3.0386842740188242E-5	0.0	0.232778408811212	0.0
48-49	3.0386842740188242E-5	3.0386842740188242E-5	0.0	0.28344846908047594	0.0
50-51	3.0386842740188242E-5	6.0773685480376484E-5	0.0	0.34180640056300743	0.0
52-53	3.0386842740188242E-5	6.0773685480376484E-5	0.0	0.3967610056586378	0.0
54-55	3.0386842740188242E-5	6.0773685480376484E-5	0.0	0.45885651879821254	0.0
56-57	3.0386842740188242E-5	6.0773685480376484E-5	0.0	0.525768346512107	0.0
58-59	3.0386842740188242E-5	6.0773685480376484E-5	0.0	0.6033763428705478	0.0
60-61	3.0386842740188242E-5	6.0773685480376484E-5	0.0	0.6889912722910281	0.0
62-63	9.116052822056472E-5	6.0773685480376484E-5	0.0	0.7791642281225368	0.0
64-65	9.116052822056472E-5	6.0773685480376484E-5	0.0	0.8737584695727427	0.0
66-67	9.116052822056472E-5	6.0773685480376484E-5	0.0	0.981616567879041	0.0
68-69	9.116052822056472E-5	6.0773685480376484E-5	0.0	1.1035133875313061	0.0
70-71	9.116052822056472E-5	6.0773685480376484E-5	0.0	1.2400110851202317	0.0
72-73	1.2154737096075297E-4	6.0773685480376484E-5	0.0	1.398569630538534	0.0
74-75	1.8232105644112943E-4	6.0773685480376484E-5	0.0	1.5536336890417144	0.0
76-77	1.8232105644112943E-4	6.0773685480376484E-5	0.0	1.7145776016151215	0.0
78-79	1.8232105644112943E-4	6.0773685480376484E-5	0.0	1.8987370620420323	0.0
80-81	1.8232105644112943E-4	6.0773685480376484E-5	0.0	2.1088164993263234	0.0
82-83	1.8232105644112943E-4	6.0773685480376484E-5	0.0	2.342871155532624	0.0
84-85	1.8232105644112943E-4	6.0773685480376484E-5	0.0	2.58443136189575	0.0
86-87	1.8232105644112943E-4	6.0773685480376484E-5	0.0	2.831704294694032	0.0
88	1.8232105644112943E-4	6.0773685480376484E-5	0.0	3.030601373849934	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAACG	350	0.0	38.94269	2
GGTATCA	4070	0.0	37.761513	1
GTATCAA	7435	0.0	36.348198	1
TACCTGG	3315	0.0	35.870136	2
GTACCTG	3490	0.0	34.4755	1
ATAACGC	375	0.0	33.83986	3
TCAACGC	8095	0.0	31.294495	4
ATCAACG	8185	0.0	31.065233	3
CAACGCA	8425	0.0	30.124502	5
AACGCAG	8840	0.0	28.763453	6
TATCAAC	9260	0.0	28.321697	2
ACCTGGG	4000	0.0	28.19988	3
GTCTCGC	730	0.0	26.39715	1
TAACGCA	550	0.0	25.636255	4
CGCAGAG	9785	0.0	25.601322	8
ACGCAGA	9950	0.0	25.318485	7
GTACATG	11965	0.0	25.179167	1
TACATGG	11535	0.0	24.28425	2
TCTGTCG	715	0.0	22.349556	8
ACATGGG	12285	0.0	21.806992	3
>>END_MODULE