FastQCFastQC Report
Wed 25 May 2016
SRR2431374_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431374_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3290898
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT272100.8268259909605221No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172740.5249023214940116No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171430.520921645095047No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168690.5125956501842355No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA75640.22984607848678387No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71810.21820791771729173No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42520.1292048553312804No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG5250.058.188442
GGTATCA72750.057.8227921
ATAACGC5750.053.945933
GTATCAA165700.050.376681
TAACGCA7550.040.4621544
ATCAACG219500.037.577273
TCAACGC221600.037.1575434
TATCAAC225550.036.6735082
CAACGCA227650.036.0874675
AACGCAG237200.034.7930456
TACCTGG27900.033.6905752
GTACCTG30650.031.7430671
TATCACG3850.031.7391452
ACGCAGA265200.030.9955187
CGCAGAG264950.030.971558
GCAGAGT281500.028.8334479
GTATAAC12200.026.9679051
ACCTGGG34850.026.7020783
GGACCGT1807.512426E-926.1101936
AGAGTAC268300.024.9793710-11
Produced by FastQC (version 0.11.5)