##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431374_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3290898
Sequences flagged as poor quality	0
Sequence length	100
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.738732102909296	34.0	31.0	34.0	31.0	34.0
2	32.85296566469091	34.0	31.0	34.0	31.0	34.0
3	32.931853858734	34.0	31.0	34.0	31.0	34.0
4	36.332572750659544	37.0	37.0	37.0	35.0	37.0
5	36.297462273215395	37.0	37.0	37.0	35.0	37.0
6	36.333374051702606	37.0	37.0	37.0	35.0	37.0
7	36.198005529189906	37.0	37.0	37.0	35.0	37.0
8	36.24941702842203	37.0	37.0	37.0	35.0	37.0
9	38.05549336381741	39.0	39.0	39.0	35.0	39.0
10-11	38.04859463891011	39.0	39.0	39.0	35.0	39.0
12-13	38.03066731329868	39.0	39.0	39.0	35.0	39.0
14-15	39.491784005459905	41.0	39.0	41.0	36.0	41.0
16-17	39.52365661287588	41.0	39.0	41.0	36.5	41.0
18-19	39.605888271225666	41.0	40.0	41.0	37.0	41.0
20-21	39.58855576806087	41.0	40.0	41.0	37.0	41.0
22-23	39.47878800862257	41.0	39.5	41.0	36.0	41.0
24-25	39.43681390307448	41.0	39.0	41.0	36.0	41.0
26-27	39.33390323856892	41.0	39.0	41.0	35.5	41.0
28-29	39.22650656446964	41.0	39.0	41.0	35.0	41.0
30-31	39.00985703598228	41.0	39.0	41.0	35.0	41.0
32-33	38.74124448706706	41.0	38.0	41.0	35.0	41.0
34-35	38.585281129952975	41.0	38.0	41.0	35.0	41.0
36-37	38.440514108914954	41.0	38.0	41.0	35.0	41.0
38-39	38.30688705635969	41.0	38.0	41.0	35.0	41.0
40-41	38.19790662001679	41.0	38.0	41.0	35.0	41.0
42-43	38.04904269290631	41.0	38.0	41.0	34.0	41.0
44-45	37.815769282426864	40.0	38.0	41.0	33.5	41.0
46-47	37.454028049486794	40.0	38.0	41.0	33.0	41.0
48-49	37.147655138506266	40.0	38.0	41.0	32.0	41.0
50-51	37.008634573298835	40.0	37.5	41.0	31.5	41.0
52-53	36.91086627419021	40.0	37.0	41.0	31.0	41.0
54-55	36.77659167801615	40.0	37.0	41.0	31.0	41.0
56-57	36.63448229024418	40.0	36.5	41.0	31.0	41.0
58-59	36.47237486546226	40.0	36.0	41.0	31.0	41.0
60-61	36.290654708836314	40.0	36.0	41.0	31.0	41.0
62-63	36.06948863197826	39.0	35.0	41.0	30.0	41.0
64-65	35.80846504510319	39.0	35.0	41.0	29.5	41.0
66-67	35.535416472950544	39.0	35.0	41.0	29.0	41.0
68-69	35.269423725682174	38.0	35.0	41.0	29.0	41.0
70-71	35.006588779111354	37.0	35.0	40.0	29.0	41.0
72-73	34.74113494249898	37.0	35.0	40.0	29.0	41.0
74-75	34.43952987907859	36.5	35.0	39.0	29.0	41.0
76-77	34.10824644215652	36.0	35.0	39.0	29.0	41.0
78-79	33.776890228746076	36.0	35.0	38.5	29.0	40.0
80-81	33.446932569772756	35.0	35.0	37.0	28.5	39.0
82-83	33.10755179893148	35.0	34.0	37.0	28.0	39.0
84-85	32.81927683568436	35.0	34.0	36.5	27.0	39.0
86-87	32.56770948233583	35.0	34.0	36.0	27.0	37.0
88-89	32.32250665319922	35.0	34.0	36.0	26.5	37.0
90-91	32.10501358595739	35.0	34.0	35.5	26.0	37.0
92-93	31.93447259684135	35.0	34.0	35.0	26.0	36.0
94-95	31.784966139941133	35.0	34.0	35.0	25.5	36.0
96-97	31.636693388856173	35.0	34.0	35.0	25.0	36.0
98-99	31.506688144087114	35.0	34.0	35.0	25.0	36.0
100	31.395166304151633	35.0	34.0	35.0	24.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.23539642305276942
1101	2	0.22972975927290662
1101	3	0.23570174612543582
1101	4	0.13357859254418258
1101	5	0.15199028634545897
1101	6	0.16368851572416787
1101	7	0.20761637823736123
1101	8	0.20465621460179761
1101	9	0.2846665269855535
1101	10-11	0.26602040631067325
1101	12-13	0.24973719684276574
1101	14-15	0.3444879320210532
1101	16-17	0.32355754037381956
1101	18-19	0.3003470938145938
1101	20-21	0.3274619508716299
1101	22-23	0.3975665671385613
1101	24-25	0.3851599847342726
1101	26-27	0.4164207764603063
1101	28-29	0.41244741869889623
1101	30-31	0.4444725470476172
1101	32-33	0.44595843090579024
1101	34-35	0.428910716187481
1101	36-37	0.4173207063873221
1101	38-39	0.4107457205399072
1101	40-41	0.40475458921523
1101	42-43	0.41472811419359346
1101	44-45	0.4245087293410137
1101	46-47	0.46272082830205363
1101	48-49	0.3429655836730774
1101	50-51	0.36337086472060065
1101	52-53	0.2830352817175452
1101	54-55	0.32368078940817213
1101	56-57	0.28900229953500656
1101	58-59	0.3251819625899657
1101	60-61	0.31247452084200944
1101	62-63	0.29436400408143015
1101	64-65	0.39334168872174047
1101	66-67	0.37269079792203286
1101	68-69	0.3043265172640375
1101	70-71	0.28842101058368996
1101	72-73	0.23619426989478853
1101	74-75	0.21491696194384247
1101	76-77	0.18347850768556384
1101	78-79	0.17825312047524733
1101	80-81	0.22784518758650307
1101	82-83	0.2978445181834388
1101	84-85	0.25354372981001205
1101	86-87	0.24113755034175455
1101	88-89	0.2414598125820362
1101	90-91	0.2029541503784884
1101	92-93	0.2424486043744949
1101	94-95	0.3083936130744185
1101	96-97	0.34597975801698055
1101	98-99	0.3731114265506861
1101	100	0.4076219128144274
1102	1	0.12166009640803566
1102	2	0.135434314402751
1102	3	0.18043527061848863
1102	4	0.10935306998387517
1102	5	0.11187747012292704
1102	6	0.08163238937332551
1102	7	0.15532880982709685
1102	8	0.16198735783070362
1102	9	0.18774817090851315
1102	10-11	0.20589333760133854
1102	12-13	0.195755247944426
1102	14-15	0.3000056378803393
1102	16-17	0.29590012978102465
1102	18-19	0.24484112646681666
1102	20-21	0.25304846302369555
1102	22-23	0.2748585024739043
1102	24-25	0.28042731781223296
1102	26-27	0.353825660855847
1102	28-29	0.24201130272385285
1102	30-31	0.2399464020208839
1102	32-33	0.261375104236933
1102	34-35	0.2769484328653533
1102	36-37	0.17876302154349588
1102	38-39	0.14594414882641615
1102	40-41	0.08806021188995317
1102	42-43	0.0756983270203122
1102	44-45	0.08154658599544717
1102	46-47	0.15717824023226967
1102	48-49	0.09771265489638381
1102	50-51	0.11908602427173776
1102	52-53	0.09061503017362327
1102	54-55	0.12441560305907728
1102	56-57	0.06315679461228285
1102	58-59	0.09543234079323781
1102	60-61	0.13197371563796167
1102	62-63	0.14708763695826121
1102	64-65	0.13325614759488502
1102	66-67	0.14028198306800022
1102	68-69	0.12664248040167791
1102	70-71	0.15927022908709176
1102	72-73	0.17872189854647758
1102	74-75	0.1541975683052783
1102	76-77	0.19170375679909313
1102	78-79	0.18485009165011235
1102	80-81	0.1691091641188862
1102	82-83	0.17109394149179025
1102	84-85	0.1470312415790218
1102	86-87	0.14850029447499935
1102	88-89	0.19352861905554875
1102	90-91	0.20944277301371983
1102	92-93	0.20173930639803217
1102	94-95	0.17970028561847684
1102	96-97	0.2135493547841989
1102	98-99	0.30189449171912486
1102	100	0.24659551507986066
1103	1	0.15110000187582528
1103	2	0.1807925170309801
1103	3	0.15926441410298509
1103	4	0.09788014248985633
1103	5	0.1405051468716394
1103	6	0.144379026292917
1103	7	0.1274563577656025
1103	8	0.14779933774764942
1103	9	0.20517313817056504
1103	10-11	0.20878180433145843
1103	12-13	0.21977536123441865
1103	14-15	0.26722863450356016
1103	16-17	0.2584080111395721
1103	18-19	0.3006768906074413
1103	20-21	0.3134609265615964
1103	22-23	0.2934309514489968
1103	24-25	0.27435827805617663
1103	26-27	0.26970424415763716
1103	28-29	0.2768050205645949
1103	30-31	0.254293309032505
1103	32-33	0.194589841889254
1103	34-35	0.2020971000103131
1103	36-37	0.14814854957405288
1103	38-39	0.15108380240567243
1103	40-41	0.14220128710567081
1103	42-43	0.19302585203121936
1103	44-45	0.1855483950073733
1103	46-47	0.11981603409434882
1103	48-49	0.1614637910402763
1103	50-51	0.12006343870741887
1103	52-53	0.10015407226653394
1103	54-55	0.10178484494324636
1103	56-57	0.12041451015523563
1103	58-59	0.10232825395058853
1103	60-61	0.03946467530240483
1103	62-63	0.04872733746348956
1103	64-65	0.09941401915882153
1103	66-67	0.0940189595363563
1103	68-69	0.1083440287067674
1103	70-71	0.08069631249438203
1103	72-73	0.08939075425194432
1103	74-75	0.04034036057062451
1103	76-77	0.010254569437236682
1103	78-79	-0.037963551776755367
1103	80-81	-0.058376290404815734
1103	82-83	0.0046313030680522616
1103	84-85	-0.014620854941078676
1103	86-87	-0.03122822146124804
1103	88-89	-0.050239360047598325
1103	90-91	0.03253249232082567
1103	92-93	0.03504928172567645
1103	94-95	0.09888526023750899
1103	96-97	0.09771464254118456
1103	98-99	0.09371403522075994
1103	100	0.15406096465270736
1104	1	0.08251699882179508
1104	2	0.12986959882154991
1104	3	0.11431649053790949
1104	4	0.027112246162552367
1104	5	0.050436486286002946
1104	6	0.05717847496731565
1104	7	6.705167966103431E-4
1104	8	0.07043718399923904
1104	9	0.14342919703827306
1104	10-11	0.168854644591363
1104	12-13	0.1805654035448896
1104	14-15	0.2026964819398529
1104	16-17	0.2268363557613995
1104	18-19	0.2186243105846728
1104	20-21	0.18135980500401416
1104	22-23	0.20101851929444337
1104	24-25	0.20851718151373433
1104	26-27	0.24749946248445553
1104	28-29	0.20041146963425405
1104	30-31	0.1833749653996719
1104	32-33	0.17102666607768668
1104	34-35	0.11488121564051568
1104	36-37	0.13457181603143198
1104	38-39	0.11729890291181277
1104	40-41	0.11711157787610205
1104	42-43	0.11514386003201338
1104	44-45	0.1512514329945276
1104	46-47	0.17385003134121746
1104	48-49	0.187842742815711
1104	50-51	0.17609383325678607
1104	52-53	0.1539094973684172
1104	54-55	0.12723210678480967
1104	56-57	0.1161543264802205
1104	58-59	0.1512097367704186
1104	60-61	0.09166547824719373
1104	62-63	0.09710798452319835
1104	64-65	0.14741614534094793
1104	66-67	0.15892168251026817
1104	68-69	0.11849192662067054
1104	70-71	0.1793840223299341
1104	72-73	0.1589959602529305
1104	74-75	0.1494236559572215
1104	76-77	0.13402814432032528
1104	78-79	0.13947550308566292
1104	80-81	0.1606305162436641
1104	82-83	0.19104822662508525
1104	84-85	0.2034110147257664
1104	86-87	0.145843408230796
1104	88-89	0.15293496452088817
1104	90-91	0.15852293708220344
1104	92-93	0.17247188698375027
1104	94-95	0.17482082930051135
1104	96-97	0.14357372461342166
1104	98-99	0.15531132954566118
1104	100	0.09795285646903906
1105	1	-0.0384388004229379
1105	2	0.004637135518372304
1105	3	0.08085023209633135
1105	4	-0.13700186845421314
1105	5	-0.138942061670015
1105	6	-0.09307637793263268
1105	7	-0.5049749387627429
1105	8	-0.41902657921141184
1105	9	-0.36596452280977587
1105	10-11	-0.36398084653586693
1105	12-13	-0.2259911760189226
1105	14-15	-0.2122210020667552
1105	16-17	-0.1584884726672442
1105	18-19	-0.16160592653274364
1105	20-21	-0.1555196710433009
1105	22-23	-0.1898621049960596
1105	24-25	-0.18655954304903588
1105	26-27	-0.19921329718068392
1105	28-29	-0.24168534627700922
1105	30-31	-0.2872431266631068
1105	32-33	-0.2571278796667684
1105	34-35	-0.26951281254351755
1105	36-37	-0.22211474476617354
1105	38-39	-0.28569461216590497
1105	40-41	-0.2684415733440204
1105	42-43	-0.25432153119260903
1105	44-45	-0.28084089354489805
1105	46-47	-0.3230435277316346
1105	48-49	-0.3151164456580915
1105	50-51	-0.32073830088451416
1105	52-53	-0.38235435947066065
1105	54-55	-0.3691360918300717
1105	56-57	-0.37321669367315025
1105	58-59	-0.36404060473370237
1105	60-61	-0.3462223313287396
1105	62-63	-0.32969175495932745
1105	64-65	-0.3413156723066848
1105	66-67	-0.3232142999987957
1105	68-69	-0.32912868428275743
1105	70-71	-0.28985578588861216
1105	72-73	-0.23503077018583696
1105	74-75	-0.2768558987069625
1105	76-77	-0.314708708894301
1105	78-79	-0.2907242792467599
1105	80-81	-0.25212418909550394
1105	82-83	-0.26584730667898526
1105	84-85	-0.25465449944561414
1105	86-87	-0.25218995002196465
1105	88-89	-0.20507259570109682
1105	90-91	-0.21535170267125636
1105	92-93	-0.18118155077740283
1105	94-95	-0.2186714888169412
1105	96-97	-0.2522625856303655
1105	98-99	-0.2829449855715893
1105	100	-0.24006230908007353
1106	1	0.08773958270528226
1106	2	0.08905639182705016
1106	3	0.08058203655671292
1106	4	0.05978451986032951
1106	5	0.04929594196561027
1106	6	0.0677405568217111
1106	7	0.06271998283101965
1106	8	0.07596334952999939
1106	9	0.0745678264955032
1106	10-11	0.13209077281388915
1106	12-13	0.10344389473383586
1106	14-15	0.13420745906672238
1106	16-17	0.15783073851347496
1106	18-19	0.1401239265060994
1106	20-21	0.1522075142936572
1106	22-23	0.14984565770839708
1106	24-25	0.1253240792553001
1106	26-27	0.15014565486121967
1106	28-29	0.18611899893308248
1106	30-31	0.16018608310782412
1106	32-33	0.09287639229333422
1106	34-35	0.12519805439733744
1106	36-37	0.12241650324990161
1106	38-39	0.0582719425232483
1106	40-41	0.15563836399947917
1106	42-43	0.22197742524495112
1106	44-45	0.13689157870108204
1106	46-47	0.1802476018032806
1106	48-49	0.13379312321306713
1106	50-51	0.23007547679711138
1106	52-53	0.12585394769367042
1106	54-55	0.05901392171486464
1106	56-57	0.11090207959684761
1106	58-59	0.0959717802643496
1106	60-61	0.09724421561928409
1106	62-63	0.11876825462001506
1106	64-65	0.012540736260135077
1106	66-67	0.03537437954447853
1106	68-69	0.04699039109632963
1106	70-71	0.05563579302538102
1106	72-73	0.08631047210343468
1106	74-75	0.10904066120602351
1106	76-77	0.06656895286656095
1106	78-79	0.045181684712645165
1106	80-81	0.04075889739117855
1106	82-83	0.07326064087887829
1106	84-85	0.09683363355708963
1106	86-87	0.05647589922445917
1106	88-89	0.07134595514027353
1106	90-91	0.06357432082362635
1106	92-93	0.029290284963728652
1106	94-95	0.05494964492604382
1106	96-97	0.09009911693128103
1106	98-99	0.10009126563473458
1106	100	0.12958159294057836
1107	1	-0.013207133615132705
1107	2	0.026246564735643574
1107	3	0.03872709463802693
1107	4	-0.05939016941304942
1107	5	-0.11104443319935342
1107	6	-0.05779820355859755
1107	7	-0.009056552250100935
1107	8	-0.014586380545722477
1107	9	0.039842074713561715
1107	10-11	0.020134789104055528
1107	12-13	0.03263897843212504
1107	14-15	0.051752194724805634
1107	16-17	0.04009841235112077
1107	18-19	0.030133983001981335
1107	20-21	0.07947574698754778
1107	22-23	0.1035127753728986
1107	24-25	0.09620991123257028
1107	26-27	0.07143687816434863
1107	28-29	0.06267467351849376
1107	30-31	0.0869540428625939
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2216	44-45	-0.7679897132134812
2216	46-47	-0.6609245838939515
2216	48-49	-0.7782834130540834
2216	50-51	-0.6850006133172144
2216	52-53	-0.7371651169478284
2216	54-55	-0.7529618410783101
2216	56-57	-0.7674018011393642
2216	58-59	-0.8347618863306963
2216	60-61	-0.804978774434737
2216	62-63	-0.8442396638298391
2216	64-65	-0.721564191745081
2216	66-67	-0.7681057498703581
2216	68-69	-0.7160396671537796
2216	70-71	-0.6616258737796201
2216	72-73	-0.7589867581480476
2216	74-75	-0.6925042574122813
2216	76-77	-0.5692204996166339
2216	78-79	-0.5485576344740082
2216	80-81	-0.6010371393557037
2216	82-83	-0.7022436918693558
2216	84-85	-0.6716291059677744
2216	86-87	-0.5745777950552622
2216	88-89	-0.5228033546960873
2216	90-91	-0.5551280427488194
2216	92-93	-0.7306232645329942
2216	94-95	-0.6586554835732947
2216	96-97	-0.788272129220573
2216	98-99	-0.7743725193891784
2216	100	-0.7762353763694776
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	56.0
3	368.0
4	225.0
5	346.0
6	566.0
7	950.0
8	1760.0
9	2543.0
10	3456.0
11	5153.0
12	8750.0
13	13879.0
14	12504.0
15	14290.0
16	18286.0
17	28396.0
18	34624.0
19	17321.0
20	10249.0
21	9228.0
22	8944.0
23	9192.0
24	10261.0
25	11906.0
26	14361.0
27	17617.0
28	21577.0
29	26928.0
30	33135.0
31	40905.0
32	51890.0
33	67001.0
34	95728.0
35	161616.0
36	239552.0
37	529676.0
38	1076413.0
39	682819.0
40	8427.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.06006841952834	21.86671593861388	14.807871315126416	23.26534432673136
2	12.789066324470197	23.1759912267081	41.71734940352129	22.317593045300416
3	14.294031634053342	30.609860450869736	32.67450326416853	22.42160465090839
4	9.641263100062476	19.502592663721913	41.443693129745014	29.4124511064706
5	10.246443371991475	38.81669987948578	36.30926877709367	14.627587971429076
6	25.18376443147129	41.7228367454719	19.959931909162787	13.133466913894019
7	22.29601160534298	34.853982104580574	26.04729772846196	16.80270856161449
8	23.434849697559752	34.845200307028655	23.44727791624049	18.272672079171095
9	24.78232385203066	15.850597618036172	23.92140382351565	35.44567470641752
10-11	21.412301444772826	27.5907062449216	31.543502715672133	19.453489594633442
12-13	23.545989574881993	26.084217742391285	31.373245235798862	18.99654744692786
14-15	20.836242873525705	26.81585694846817	30.430690954262328	21.917209223743793
16-17	18.58868005024768	30.12662501238264	30.279896247164146	21.004798690205533
18-19	18.34298723327189	27.647134611890127	34.7921904598684	19.21768769496958
20-21	20.028499819806022	27.181015637677007	34.231370890255484	18.55911365226148
22-23	19.313117052790037	27.528023907170557	34.33035172341389	18.82850731662552
24-25	19.12271295153553	27.249573862190395	34.42057032804597	19.207142858228103
26-27	19.007165256709452	27.171091015169196	34.44672853461688	19.37501519350447
28-29	19.111118741656835	27.18169710292151	34.4389639569147	19.26822019850696
30-31	19.630845741361682	27.709815696356543	33.80308075748899	18.85625780479278
32-33	18.764534037890567	27.296330505370104	34.61046233453513	19.328673122204204
34-35	19.03203701312021	26.96273617515897	34.26874017123886	19.73648664048196
36-37	19.82646329198895	27.62529842907754	33.32043363057348	19.227804648360028
38-39	19.533313034404255	27.520121161512012	33.5970826670848	19.34948313699893
40-41	19.302078485516606	27.42990997134271	33.590536362822796	19.67747518031789
42-43	19.194391896029042	27.602400026375918	33.45964704582862	19.743561031766422
44-45	19.6552808115394	27.61990974150847	33.05665974298984	19.6681497039623
46-47	19.74357443274458	28.33061285327686	32.19772093511583	19.728091778862726
48-49	19.85796842273994	28.020985666935346	32.10097490972988	20.020071000594836
50-51	19.892447550556323	27.949415646581084	31.722624438995346	20.43551236386725
52-53	19.822559016584233	28.271562434193477	31.57567678356387	20.33020176565842
54-55	20.099547841306883	28.737480552314278	31.230992925904317	19.931978680474526
56-57	19.747392027032365	28.33308517200619	31.402668595234694	20.51685420572675
58-59	19.50823702153288	27.776086231195723	31.972114398788893	20.7435623484825
60-61	20.21124725491608	28.30870924308157	31.22645198872157	20.25359151328078
62-63	19.8929137274993	28.190998323254018	31.59241033906247	20.32367761018421
64-65	19.81316345872768	27.855299678081792	31.901839558685808	20.429697304504728
66-67	19.843412252168537	28.091108416450904	31.416140501426597	20.649338829953958
68-69	20.337156480091696	28.221532537466093	31.182932930765688	20.258378051676523
70-71	20.51210835452608	27.91939533740303	31.19361789604862	20.37487841202227
72-73	20.102084691246606	28.096475274963197	31.498209453659264	20.303230580130936
74-75	20.07171443070593	27.974325626057865	31.337291807841787	20.616668135394413
76-77	20.191857147284956	28.254609852787365	30.968544641169583	20.584988358758096
78-79	20.27140034604957	28.35265149696523	31.060805419133853	20.315142737851343
80-81	20.149954221845828	28.128237174899894	31.094123713752374	20.6276848895019
82-83	19.825887272811805	28.151607339912875	31.394288994369703	20.628216392905614
84-85	20.03908706497104	28.05676695002231	31.062639125930207	20.84150685907644
86-87	20.15708822796998	28.239583857191192	30.916671617202585	20.686656297636247
88-89	19.678067878532257	28.11365699246608	31.173095453483278	21.035179675518386
90-91	19.678820605073476	28.053338965127445	31.0058526090603	21.261987820738778
92-93	19.78286735487835	28.312694225217918	30.934200032545284	20.970238387358453
94-95	19.66576305529026	27.898193455892656	31.17706903556477	21.258974453252318
96-97	19.56470146364647	28.081646470091666	31.160041472920746	21.193610593341116
98-99	19.472240640472975	27.893505505318128	31.13224432344785	21.502009530761047
100	19.708665346212122	27.998113520599638	30.809691058610312	21.483530074577928
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6356.0
1	3933.5
2	7961.0
3	11226.5
4	5046.0
5	2247.0
6	3727.5
7	9581.0
8	15802.0
9	14889.5
10	8696.5
11	4453.5
12	3924.5
13	4158.5
14	4545.0
15	4982.5
16	5438.5
17	6033.0
18	6709.5
19	7615.5
20	8998.5
21	10714.0
22	13350.5
23	17642.5
24	22960.0
25	29054.5
26	37167.5
27	47317.0
28	59045.5
29	70362.0
30	79436.5
31	87756.0
32	95201.0
33	101491.5
34	107310.5
35	112209.0
36	115627.5
37	117673.5
38	119351.5
39	119853.0
40	119556.0
41	117381.0
42	112338.0
43	109059.0
44	105711.5
45	100237.5
46	95910.0
47	92746.0
48	89098.0
49	86300.5
50	84806.5
51	80403.0
52	72819.0
53	65996.0
54	61071.0
55	57178.0
56	52454.0
57	46916.5
58	42548.0
59	38694.0
60	34002.5
61	28410.0
62	23011.0
63	18176.0
64	13775.0
65	10090.5
66	7363.0
67	5376.0
68	3749.5
69	2636.5
70	1886.5
71	1357.5
72	947.5
73	632.5
74	470.5
75	346.0
76	249.5
77	195.0
78	119.5
79	65.5
80	39.5
81	23.0
82	14.5
83	9.5
84	5.5
85	4.5
86	6.0
87	3.0
88	1.0
89	2.0
90	3.0
91	3.0
92	3.0
93	1.0
94	2.0
95	3.0
96	5.0
97	7.5
98	4.5
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009632629148639672
2	3.038684274018824E-5
3	4.2541579836263534E-4
4	0.00224862636277393
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	7.596710685047061E-5
24-25	7.596710685047061E-5
26-27	5.469631693233883E-4
28-29	9.419921249458354E-4
30-31	0.0015649224011196944
32-33	0.003995869820334753
34-35	0.005256923794052566
36-37	0.003783161921153436
38-39	0.002552494790175812
40-41	0.003418519808271177
42-43	5.317697479532942E-4
44-45	5.01382905213106E-4
46-47	0.003600840864712306
48-49	0.0024461408405851534
50-51	0.0011698934454972471
52-53	3.038684274018824E-5
54-55	5.469631693233883E-4
56-57	8.508315967252707E-4
58-59	7.292842257645177E-4
60-61	4.558026411028236E-4
62-63	0.0
64-65	0.0
66-67	8.356381753551767E-4
68-69	6.837039616542354E-4
70-71	0.002157465834553365
72-73	9.116052822056472E-5
74-75	0.0020663053063328003
76-77	3.6464211288225886E-4
78-79	0.0012154737096075298
80-81	8.204447539850825E-4
82-83	0.001261053973717812
84-85	0.0012002802882374356
86-87	2.5828816329160004E-4
88-89	0.0014889552942692237
90-91	0.0017624368789309179
92-93	0.0029931040099085418
94-95	8.812184394654589E-4
96-97	0.002309400048254306
98-99	0.0024309474192150595
100	0.0034033263869010827
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3290898.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.24457273553924
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.22927697128448	57.631757229644485
2	11.688802012849306	16.18772202340123
3	2.5492281623624753	5.295606447245804
4	0.9285511230122183	2.571885031043448
5	0.44259761752428134	1.5323741459618239
6	0.2656410596113118	1.1036521004280715
7	0.1756663001763414	0.851475652982062
8	0.11892658356603406	0.6588016372741945
9	0.0817038089352616	0.5091790806529489
>10	0.4666705670649551	5.918135700071782
>50	0.03291009239355538	1.5658368056169436
>100	0.018312451006608037	2.307558938610806
>500	0.0010982373171518115	0.5489374752294532
>1k	3.5143594059388987E-4	0.4807993858557685
>5k	8.785898514847247E-5	0.44852505026213507
>10k+	1.7571797029694494E-4	2.387753295718993
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27210	0.8268259909605221	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17274	0.5249023214940116	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17143	0.520921645095047	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16869	0.5125956501842355	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7564	0.22984607848678387	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7181	0.21820791771729173	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4252	0.1292048553312804	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.0386842740188242E-5	0.0
5	0.0	0.0	0.0	3.0386842740188242E-5	0.0
6	0.0	0.0	0.0	3.0386842740188242E-5	0.0
7	0.0	0.0	0.0	3.0386842740188242E-5	0.0
8	0.0	0.0	0.0	3.0386842740188242E-5	0.0
9	0.0	0.0	0.0	3.0386842740188242E-5	0.0
10-11	0.0	0.0	0.0	3.0386842740188242E-5	0.0
12-13	0.0	0.0	0.0	6.0773685480376484E-5	0.0
14-15	0.0	0.0	0.0	9.116052822056472E-5	0.0
16-17	0.0	0.0	0.0	1.0635394959065885E-4	0.0
18-19	0.0	0.0	0.0	1.367407923308471E-4	0.0
20-21	0.0	0.0	0.0	1.9751447781122356E-4	0.0
22-23	0.0	0.0	0.0	7.292842257645178E-4	0.0
24-25	0.0	0.0	0.0	0.0017928237216711062	0.0
26-27	0.0	0.0	0.0	0.0027196224252468474	0.0
28-29	0.0	0.0	0.0	0.004892281681170307	0.0
30-31	0.0	0.0	0.0	0.010377106795774284	0.0
32-33	0.0	0.0	0.0	0.020100896472634522	0.0
34-35	0.0	0.0	0.0	0.033729395441608946	0.0
36-37	0.0	0.0	0.0	0.0527059787328565	0.0
38-39	0.0	0.0	0.0	0.08253066488235127	0.0
40-41	0.0	0.0	0.0	0.11846310642262385	0.0
42-43	0.0	0.0	0.0	0.15387897163631326	0.0
44-45	0.0	0.0	0.0	0.19092053293660272	0.0
46-47	0.0	0.0	0.0	0.2346471996397336	0.0
48-49	0.0	0.0	0.0	0.2858642230783209	0.0
50-51	0.0	0.0	0.0	0.3450122124720973	0.0
52-53	0.0	0.0	0.0	0.40081764916445295	0.0
54-55	0.0	0.0	0.0	0.46403747548541463	0.0
56-57	0.0	0.0	0.0	0.5319672624311054	0.0
58-59	0.0	0.0	0.0	0.610821119341894	0.0
60-61	0.0	0.0	0.0	0.6973780408873201	0.0
62-63	0.0	0.0	0.0	0.7889335980635073	0.0
64-65	0.0	0.0	0.0	0.884940827701132	0.0
66-67	0.0	0.0	0.0	0.9942423010375891	0.0
68-69	0.0	0.0	0.0	1.1175521088772729	0.0
70-71	0.0	0.0	1.5193421370094121E-5	1.256526334149524	0.0
72-73	0.0	6.0773685480376484E-5	3.0386842740188242E-5	1.4176829546221121	0.0
74-75	0.0	9.116052822056472E-5	3.0386842740188242E-5	1.575846471084792	0.0
76-77	0.0	9.116052822056472E-5	3.0386842740188242E-5	1.7397531008253675	0.0
78-79	0.0	9.116052822056472E-5	3.0386842740188242E-5	1.9274678218528802	0.0
80-81	0.0	9.116052822056472E-5	3.0386842740188242E-5	2.1418621908062785	0.0
82-83	0.0	9.116052822056472E-5	3.0386842740188242E-5	2.380687581322788	0.0
84-85	0.0	9.116052822056472E-5	3.0386842740188242E-5	2.627428744373116	0.0
86-87	0.0	9.116052822056472E-5	3.0386842740188242E-5	2.8796547325380493	0.0
88	0.0	9.116052822056472E-5	3.0386842740188242E-5	3.0822286196655138	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAACG	525	0.0	58.18844	2
GGTATCA	7275	0.0	57.822792	1
ATAACGC	575	0.0	53.94593	3
GTATCAA	16570	0.0	50.37668	1
TAACGCA	755	0.0	40.462154	4
ATCAACG	21950	0.0	37.57727	3
TCAACGC	22160	0.0	37.157543	4
TATCAAC	22555	0.0	36.673508	2
CAACGCA	22765	0.0	36.087467	5
AACGCAG	23720	0.0	34.793045	6
TACCTGG	2790	0.0	33.690575	2
GTACCTG	3065	0.0	31.743067	1
TATCACG	385	0.0	31.739145	2
ACGCAGA	26520	0.0	30.995518	7
CGCAGAG	26495	0.0	30.97155	8
GCAGAGT	28150	0.0	28.833447	9
GTATAAC	1220	0.0	26.967905	1
ACCTGGG	3485	0.0	26.702078	3
GGACCGT	180	7.512426E-9	26.110193	6
AGAGTAC	26830	0.0	24.97937	10-11
>>END_MODULE