FastQCFastQC Report
Wed 25 May 2016
SRR2431373_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431373_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5824419
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA206460.35447312427213773No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63150.050.7588841
GTATCAA123600.041.6006281
TCAACGC144850.034.2968144
ATCAACG146200.034.0765653
CAACGCA165950.029.9644095
TATCAAC175350.029.0013892
AACGCAG175250.028.4011066
GTACATG298450.027.4332711
CGCAGAG178900.027.164868
ACGCAGA181350.027.0052037
TACATGG286350.026.9180742
ACATGGG301200.024.71713
GCAGAGT203900.023.0043859
CATGGGG170650.022.3087924
CATGGGT48250.021.2352074
GTCGTAC2551.3487806E-820.2746561
GTGGTAT28800.020.0730631
AGAGTAC188000.019.02497710-11
ATGGGCG10750.018.7999765
CAGAGTA200550.018.3265810-11
Produced by FastQC (version 0.11.5)