FastQCFastQC Report
Wed 25 May 2016
SRR2431373_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431373_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5824419
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT316700.5437452216263974No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219200.37634655061732336No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177350.30449389029189006No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA107750.063.427841
GTATCAA239700.048.8862881
ATCAACG321100.036.1826173
TCAACGC323200.035.9184344
TATCAAC340100.034.2856142
CAACGCA340900.034.1356355
AACGCAG354550.032.8346826
ACGCAGA384600.030.1470077
CGCAGAG386300.029.941348
GCAGAGT424900.026.9669179
TACATGG266950.026.0921612
GTACATG279800.025.9881531
GTGGTAT35900.025.9240911
ACATGGG274150.024.7211573
TGGTATC39500.023.2022062
AGAGTAC407050.023.1730910-11
AGTACTT281500.022.53091412-13
GAGTACT269750.021.89199612-13
CAGAGTA418700.021.4787910-11
CATGGGG160100.021.4007024
Produced by FastQC (version 0.11.5)