##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431373_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5824419
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.735441423427815	34.0	31.0	34.0	31.0	34.0
2	32.869320012863085	34.0	31.0	34.0	31.0	34.0
3	32.94754635612582	34.0	31.0	34.0	31.0	34.0
4	36.33805105710973	37.0	37.0	37.0	35.0	37.0
5	36.289279153852085	37.0	37.0	37.0	35.0	37.0
6	36.32155808158719	37.0	37.0	37.0	35.0	37.0
7	36.191094768422396	37.0	37.0	37.0	35.0	37.0
8	36.245436669305555	37.0	37.0	37.0	35.0	37.0
9	38.05741722908328	39.0	39.0	39.0	35.0	39.0
10-11	38.05014019424083	39.0	39.0	39.0	35.0	39.0
12-13	38.025031509580614	39.0	39.0	39.0	35.0	39.0
14-15	39.505802638855485	41.0	39.0	41.0	36.0	41.0
16-17	39.52160103866154	41.0	39.5	41.0	36.5	41.0
18-19	39.59404835057368	41.0	40.0	41.0	37.0	41.0
20-21	39.59385717270684	41.0	40.0	41.0	37.0	41.0
22-23	39.5219719769474	41.0	40.0	41.0	36.0	41.0
24-25	39.48849490395523	41.0	40.0	41.0	36.0	41.0
26-27	39.40048406544928	41.0	39.0	41.0	36.0	41.0
28-29	39.31864706162108	41.0	39.0	41.0	36.0	41.0
30-31	39.152381293310114	41.0	39.0	41.0	35.0	41.0
32-33	38.95818338275457	41.0	39.0	41.0	35.0	41.0
34-35	38.862644239708715	41.0	39.0	41.0	35.0	41.0
36-37	38.75237289762292	41.0	38.0	41.0	35.0	41.0
38-39	38.62942844599607	41.0	38.0	41.0	35.0	41.0
40-41	38.533890762323246	41.0	38.0	41.0	35.0	41.0
42-43	38.39426464682572	41.0	38.0	41.0	35.0	41.0
44-45	38.18133259300198	41.0	38.0	41.0	34.0	41.0
46-47	37.87170608776601	40.0	38.0	41.0	33.5	41.0
48-49	37.5987988673205	40.0	38.0	41.0	33.0	41.0
50-51	37.43501334639558	40.0	38.0	41.0	33.0	41.0
52-53	37.30861928030933	40.0	37.0	41.0	33.0	41.0
54-55	37.15606895726424	40.0	37.0	41.0	32.5	41.0
56-57	36.98532051008006	40.0	36.5	41.0	32.0	41.0
58-59	36.791458856239565	40.0	36.0	41.0	32.0	41.0
60-61	36.593189466623194	39.5	35.5	41.0	31.0	41.0
62-63	36.36698690461658	39.0	35.0	41.0	31.0	41.0
64-65	36.10297130065677	39.0	35.0	41.0	31.0	41.0
66-67	35.8161669000805	38.5	35.0	41.0	31.0	41.0
68-69	35.54383003695304	37.5	35.0	40.5	30.5	41.0
70-71	35.288487229370006	37.0	35.0	40.0	30.0	41.0
72-73	35.024415053243935	36.5	35.0	39.0	30.0	41.0
74-75	34.72185457467947	36.0	35.0	39.0	30.0	41.0
76-77	34.40273167160535	36.0	35.0	39.0	30.0	40.5
78-79	34.08020714169087	35.0	35.0	37.5	30.0	39.5
80-81	33.770034401714575	35.0	35.0	37.0	30.0	39.0
82-83	33.45430368247889	35.0	34.5	37.0	29.0	39.0
84-85	33.195071903309156	35.0	34.0	36.0	29.0	38.0
86-87	32.97360801137418	35.0	34.0	36.0	29.0	37.0
88-89	32.75065135252117	35.0	34.0	36.0	29.0	37.0
90-91	32.554325847779836	35.0	34.0	35.0	29.0	36.5
92-93	32.41021310451738	35.0	34.0	35.0	29.0	36.0
94-95	32.27652071391155	35.0	34.0	35.0	29.0	36.0
96-97	32.1443302756893	35.0	34.0	35.0	29.0	36.0
98-99	32.02968536432561	35.0	34.0	35.0	29.0	36.0
100	31.9263799530906	35.0	34.0	35.0	27.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.1776564319724585
1101	2	0.1559325748712368
1101	3	0.17575689962011865
1101	4	0.12888409965060532
1101	5	0.16041054177871672
1101	6	0.15674314693805513
1101	7	0.14707134079653628
1101	8	0.18259093637061596
1101	9	0.25305443245018466
1101	10-11	0.18979017270137177
1101	12-13	0.2240550786833495
1101	14-15	0.2819933329671258
1101	16-17	0.27637655357700197
1101	18-19	0.23843600546020127
1101	20-21	0.251810154422877
1101	22-23	0.27439723305904096
1101	24-25	0.25104991257855147
1101	26-27	0.2225726919985931
1101	28-29	0.24552007768912887
1101	30-31	0.2532044440529049
1101	32-33	0.23478750115286573
1101	34-35	0.2465633696543179
1101	36-37	0.2357027624434309
1101	38-39	0.2509911545981325
1101	40-41	0.21313533073866608
1101	42-43	0.30099095579830504
1101	44-45	0.3159674710333249
1101	46-47	0.2980588811131355
1101	48-49	0.2755560807756652
1101	50-51	0.26747873921051735
1101	52-53	0.2370039935967796
1101	54-55	0.24207447777206426
1101	56-57	0.27054296829128077
1101	58-59	0.307485411566887
1101	60-61	0.29701246641757706
1101	62-63	0.30117590303024144
1101	64-65	0.38659506799795906
1101	66-67	0.3410034548826886
1101	68-69	0.35308192396830407
1101	70-71	0.2842806763805399
1101	72-73	0.26970944994602064
1101	74-75	0.2492251429235921
1101	76-77	0.2106873642088516
1101	78-79	0.212209259596996
1101	80-81	0.2031426723985561
1101	82-83	0.23320017506065227
1101	84-85	0.20849281373724438
1101	86-87	0.20093728753926854
1101	88-89	0.1904023105539352
1101	90-91	0.21513988703156173
1101	92-93	0.2449052659089901
1101	94-95	0.2221798805438695
1101	96-97	0.2225187265277171
1101	98-99	0.24256064774854025
1101	100	0.29774905819569497
1102	1	0.12388094198118438
1102	2	0.14028271142611004
1102	3	0.159616016984657
1102	4	0.11230049000854336
1102	5	0.16720369862724738
1102	6	0.15005136833585198
1102	7	0.15962362096507832
1102	8	0.17466021664775866
1102	9	0.22683683177304204
1102	10-11	0.21913162311646772
1102	12-13	0.20704552829325706
1102	14-15	0.32132528842904406
1102	16-17	0.31450428512764717
1102	18-19	0.2703503936669094
1102	20-21	0.2776981298320038
1102	22-23	0.295274375838396
1102	24-25	0.2742630725873596
1102	26-27	0.28996332810268655
1102	28-29	0.28210255155065767
1102	30-31	0.2981465054171366
1102	32-33	0.301911301046772
1102	34-35	0.3144163077704434
1102	36-37	0.2748103812752305
1102	38-39	0.2741126854508238
1102	40-41	0.2860729533398114
1102	42-43	0.3390233014512276
1102	44-45	0.371827422532796
1102	46-47	0.3738255452660866
1102	48-49	0.4634768780911429
1102	50-51	0.44501301406165794
1102	52-53	0.43528241181833494
1102	54-55	0.46426590854147776
1102	56-57	0.42442956103510454
1102	58-59	0.43257428270612763
1102	60-61	0.37383797856825396
1102	62-63	0.3483854076449333
1102	64-65	0.413155938059937
1102	66-67	0.4558901911970352
1102	68-69	0.43183346914916854
1102	70-71	0.4550407624712207
1102	72-73	0.41420183778877373
1102	74-75	0.4306975508625257
1102	76-77	0.4124282075467889
1102	78-79	0.43319393891323443
1102	80-81	0.401082937779087
1102	82-83	0.4261830472689283
1102	84-85	0.47459850284842275
1102	86-87	0.4719804260573497
1102	88-89	0.44419344672105154
1102	90-91	0.4443042297425279
1102	92-93	0.4572865997369533
1102	94-95	0.44614770464342257
1102	96-97	0.4395922767373648
1102	98-99	0.40825744511170825
1102	100	0.4378911469219098
1103	1	0.12779697953778424
1103	2	0.1777955363075705
1103	3	0.14595068774047348
1103	4	0.13955709272511996
1103	5	0.12943974462735497
1103	6	0.16115007002373005
1103	7	0.1246717012868217
1103	8	0.1606999883715261
1103	9	0.20075346363253743
1103	10-11	0.231786561343597
1103	12-13	0.20821702790057373
1103	14-15	0.2842126858366427
1103	16-17	0.2807250445310885
1103	18-19	0.27297431323984966
1103	20-21	0.2853818422877126
1103	22-23	0.32077324213523184
1103	24-25	0.3031416199801953
1103	26-27	0.2624392586451876
1103	28-29	0.2489422610590566
1103	30-31	0.311587825844029
1103	32-33	0.30259732900093184
1103	34-35	0.2564150009128383
1103	36-37	0.24373109449653896
1103	38-39	0.27810344080675975
1103	40-41	0.25426169998240056
1103	42-43	0.27801291195606836
1103	44-45	0.2930061176947518
1103	46-47	0.2928323943596425
1103	48-49	0.2939749198973445
1103	50-51	0.3065573173640246
1103	52-53	0.3023459399062247
1103	54-55	0.2902125337494965
1103	56-57	0.27419833459558873
1103	58-59	0.25910123213567715
1103	60-61	0.19696948760222455
1103	62-63	0.1870012084772199
1103	64-65	0.2573364152935369
1103	66-67	0.29834543223708465
1103	68-69	0.30845372261136106
1103	70-71	0.2739855864316283
1103	72-73	0.28865428583349484
1103	74-75	0.277009452436225
1103	76-77	0.24890959350071284
1103	78-79	0.20982403908408997
1103	80-81	0.23000221889021333
1103	82-83	0.2532408362627976
1103	84-85	0.205986233590707
1103	86-87	0.19825445813311404
1103	88-89	0.18261968699733444
1103	90-91	0.22345842790655013
1103	92-93	0.26194852166990046
1103	94-95	0.26811019477103315
1103	96-97	0.26916952953151707
1103	98-99	0.3197850569492786
1103	100	0.3628489845177221
1104	1	0.09317127098670142
1104	2	0.14291267330071378
1104	3	0.1581054217205633
1104	4	0.0631152883455286
1104	5	0.1084629795974763
1104	6	0.10637667075802426
1104	7	0.10288576631104718
1104	8	0.12332147907795843
1104	9	0.179894728278839
1104	10-11	0.1879433436802742
1104	12-13	0.18806713947309106
1104	14-15	0.2307215822003741
1104	16-17	0.2756202794118323
1104	18-19	0.2527569057810197
1104	20-21	0.23009441341846326
1104	22-23	0.2249695359681212
1104	24-25	0.2267032481487874
1104	26-27	0.23203521685277195
1104	28-29	0.1970643154163625
1104	30-31	0.22067813956790872
1104	32-33	0.19085247949537631
1104	34-35	0.21378300234583492
1104	36-37	0.2557301436733468
1104	38-39	0.2494677052458698
1104	40-41	0.22432147115712553
1104	42-43	0.16839975572653998
1104	44-45	0.17303969786529905
1104	46-47	0.20955493854965823
1104	48-49	0.21825015057818575
1104	50-51	0.20787841475335966
1104	52-53	0.22902302819099418
1104	54-55	0.2667170207220906
1104	56-57	0.1980796160577043
1104	58-59	0.2122558872480056
1104	60-61	0.20920483868832918
1104	62-63	0.22801219167588727
1104	64-65	0.250837837154954
1104	66-67	0.31437211732239945
1104	68-69	0.30803708329360546
1104	70-71	0.2694890953117479
1104	72-73	0.2389362509239703
1104	74-75	0.25993756010328184
1104	76-77	0.21268156851617448
1104	78-79	0.20515443581242465
1104	80-81	0.21475139994816317
1104	82-83	0.20786024127356484
1104	84-85	0.20087626452259855
1104	86-87	0.17929636954647066
1104	88-89	0.21148894608805335
1104	90-91	0.2608891236928841
1104	92-93	0.2492278068002136
1104	94-95	0.2178794459455773
1104	96-97	0.21839846675064933
1104	98-99	0.2401798764328973
1104	100	0.2111536107474059
1105	1	-0.039365168676667395
1105	2	0.01969650829590819
1105	3	0.039853277116911556
1105	4	-0.06946408327418396
1105	5	-0.09588805652555266
1105	6	-0.0982731427750636
1105	7	-0.4219176350009448
1105	8	-0.352868563007938
1105	9	-0.3296604438209627
1105	10-11	-0.291623353748129
1105	12-13	-0.18441390272360536
1105	14-15	-0.15579979321519488
1105	16-17	-0.10468101619943582
1105	18-19	-0.1306591321209325
1105	20-21	-0.09297114048228394
1105	22-23	-0.10055064994663354
1105	24-25	-0.08858656778885177
1105	26-27	-0.11089523886839459
1105	28-29	-0.1286409988361683
1105	30-31	-0.11399195468985823
1105	32-33	-0.15167369660380814
1105	34-35	-0.16152662829534847
1105	36-37	-0.1545317304570517
1105	38-39	-0.1909646936975875
1105	40-41	-0.21544807969099367
1105	42-43	-0.1851597555642428
1105	44-45	-0.2009842679336984
1105	46-47	-0.18853759803384662
1105	48-49	-0.20249932269140913
1105	50-51	-0.155364058172907
1105	52-53	-0.1497508679897095
1105	54-55	-0.16046056957216592
1105	56-57	-0.11276762294023968
1105	58-59	-0.1285329209281798
1105	60-61	-0.09436911866560393
1105	62-63	-0.09497941152309153
1105	64-65	-0.09053087061077036
1105	66-67	-0.13393810322251198
1105	68-69	-0.12937627861999346
1105	70-71	-0.1278882712420497
1105	72-73	-0.06732361752487748
1105	74-75	-0.16009577380080486
1105	76-77	-0.09873987984821042
1105	78-79	-0.07109932493298032
1105	80-81	-0.0754489644244174
1105	82-83	-0.054379040140496215
1105	84-85	-0.08860710954084539
1105	86-87	-0.11327248174605842
1105	88-89	-0.09295088711338195
1105	90-91	-0.09988236430253039
1105	92-93	-0.10084573618856751
1105	94-95	-0.10295582168129869
1105	96-97	-0.07130553109946192
1105	98-99	-0.1105506246413448
1105	100	-0.13297810360671036
1106	1	0.12114813145342396
1106	2	0.1173247429624027
1106	3	0.10787551931929329
1106	4	0.05936590238885486
1106	5	0.10683425680919356
1106	6	0.13020694452268344
1106	7	0.10044530527802209
1106	8	0.0996252256117387
1106	9	0.15543499186460963
1106	10-11	0.15487599547766706
1106	12-13	0.11963501025498857
1106	14-15	0.1452702266020296
1106	16-17	0.19666267664336345
1106	18-19	0.1859216886713284
1106	20-21	0.15409354610140014
1106	22-23	0.1631191549243809
1106	24-25	0.1596292445500893
1106	26-27	0.19429682516772573
1106	28-29	0.18312254927995752
1106	30-31	0.21180656171930679
1106	32-33	0.1614231598966569
1106	34-35	0.16075615690163403
1106	36-37	0.24351276538574496
1106	38-39	0.18807638200626542
1106	40-41	0.2597936348548586
1106	42-43	0.2771336595092109
1106	44-45	0.3204781977945359
1106	46-47	0.4226084849917342
1106	48-49	0.4444807418690786
1106	50-51	0.4603939750892323
1106	52-53	0.39915915393301304
1106	54-55	0.4256477179669247
1106	56-57	0.3888551274107144
1106	58-59	0.3893088585146387
1106	60-61	0.40379323751846385
1106	62-63	0.36360801090302175
1106	64-65	0.3792596556648675
1106	66-67	0.3943472890135169
1106	68-69	0.37131621525857383
1106	70-71	0.3468770227845752
1106	72-73	0.39128644499174925
1106	74-75	0.38364534610606427
1106	76-77	0.3336254871264259
1106	78-79	0.3351756697408419
1106	80-81	0.31432386121490197
1106	82-83	0.33802690020837645
1106	84-85	0.3487409929419485
1106	86-87	0.3277685234972765
1106	88-89	0.3490898634393531
1106	90-91	0.3375956514893943
1106	92-93	0.3698323205026739
1106	94-95	0.38677514824683357
1106	96-97	0.3990761777729048
1106	98-99	0.39947234193288494
1106	100	0.44354977490936776
1107	1	0.0022877106174377104
1107	2	0.022753602369043335
1107	3	0.03305832551136234
1107	4	-0.06029917000076779
1107	5	-0.09777640783178043
1107	6	-0.07580720740523361
1107	7	-0.05660131711207583
1107	8	-0.01513498345811115
1107	9	0.015552062719052628
1107	10-11	0.0575494361545239
1107	12-13	0.09303878352280037
1107	14-15	0.09027106468553114
1107	16-17	0.11715992791226171
1107	18-19	0.07450611327106316
1107	20-21	0.09549826988878607
1107	22-23	0.09516666899778414
1107	24-25	0.08268885029367823
1107	26-27	0.07821579476912888
1107	28-29	0.07566170047550713
1107	30-31	0.1398267054132063
1107	32-33	0.0999131969783349
1107	34-35	0.041997131078232997
1107	36-37	0.055756199570851095
1107	38-39	0.057234689286062235
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2216	44-45	-0.5061424723435479
2216	46-47	-0.48390463318476407
2216	48-49	-0.5264106610574686
2216	50-51	-0.48866748953869177
2216	52-53	-0.5689034232278374
2216	54-55	-0.6166587874149059
2216	56-57	-0.6218681717563541
2216	58-59	-0.6404827822290571
2216	60-61	-0.6365050162076642
2216	62-63	-0.640429252122594
2216	64-65	-0.5502879976297237
2216	66-67	-0.575980614619759
2216	68-69	-0.581909780324402
2216	70-71	-0.5748720827768423
2216	72-73	-0.6035515763245698
2216	74-75	-0.4923652871347741
2216	76-77	-0.4497705776681258
2216	78-79	-0.45178184884305495
2216	80-81	-0.47629116484120715
2216	82-83	-0.5220895154897747
2216	84-85	-0.4909329075783546
2216	86-87	-0.4646361264738843
2216	88-89	-0.4242888391506199
2216	90-91	-0.43332437978715177
2216	92-93	-0.5856388605761467
2216	94-95	-0.5466774435640218
2216	96-97	-0.6094144386655849
2216	98-99	-0.5948083888022282
2216	100	-0.587384503547824
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	68.0
3	458.0
4	255.0
5	470.0
6	583.0
7	1095.0
8	2112.0
9	3565.0
10	4585.0
11	6271.0
12	9638.0
13	14128.0
14	14962.0
15	19426.0
16	25987.0
17	40818.0
18	48929.0
19	24888.0
20	15129.0
21	13597.0
22	13465.0
23	14201.0
24	15977.0
25	19732.0
26	24355.0
27	30743.0
28	38439.0
29	48546.0
30	61351.0
31	76550.0
32	96106.0
33	120877.0
34	165511.0
35	264014.0
36	472288.0
37	1099090.0
38	1915030.0
39	1084492.0
40	16688.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.142325786803475	22.002887062358244	14.844813100745153	24.009974050093124
2	13.166502329761073	22.436442995067146	40.71691638836985	23.680138286801924
3	14.80608207167197	29.63973781320195	32.097279769905796	23.456900345220287
4	10.246337000582221	19.127349634468395	40.97389589779434	29.65241746715504
5	11.28823321261743	39.45135128499512	36.02946491315271	13.23095058923474
6	26.65960330120481	41.05853304853239	19.130526152050532	13.151337498212268
7	23.86509281011548	34.65801481658514	24.845551118489244	16.631341254810135
8	24.477583085969602	35.31504172347491	21.695400004704332	18.511975185851156
9	25.868159553768365	15.837373650487713	22.612933581873147	35.68153321387078
10-11	22.912620125715545	27.371923276810957	30.004057057021484	19.711399540452017
12-13	24.108164264967886	26.072866667044387	29.815016742442463	20.00395232554526
14-15	21.61703167303039	26.685554387484828	28.49938337197238	23.198030567512397
16-17	19.959759076398864	30.028076620174478	28.628203087724284	21.383961215702374
18-19	19.825333651304963	28.19578227459254	32.154855273976686	19.824028800125816
20-21	21.467789319415377	27.172967810179866	31.706587386656075	19.652655483748678
22-23	20.87678191776579	27.41669418016889	31.804466819231397	19.902057082833927
24-25	20.44203599933899	27.548581080066015	31.61857097175037	20.390811948844625
26-27	20.248977338467725	27.799473438013333	31.730626346762246	20.220922876756696
28-29	20.32335769368454	27.515330023667854	32.00619639427885	20.155115888368755
30-31	20.758927210906485	27.616971510891368	31.583215772983376	20.04088550521877
32-33	20.081424533572704	27.577141516914182	32.04799622812949	20.29343772138363
34-35	20.63291923844819	27.439910111556998	31.64028723368454	20.286883416310275
36-37	20.869858820626707	27.60861109100816	31.142383575800668	20.379146512564457
38-39	20.53264101082017	27.572152722221134	31.35158532143265	20.543620945526044
40-41	20.51235759128679	27.542096456457166	31.37397864964943	20.57156730260661
42-43	20.51985723820745	27.67316735021494	31.349236293086612	20.457739118491
44-45	20.662908279494413	27.553348669402688	30.857674414452106	20.92606863665079
46-47	21.05023550373934	27.786573904563145	30.325284040302407	20.8379065513951
48-49	20.983078440859533	27.72830008691194	30.094255330181248	21.194366142047283
50-51	21.232340878508165	27.92927981388521	29.462941223181076	21.37543808442555
52-53	21.056438600388912	28.092738192897553	29.63642032560163	21.214402881111898
54-55	21.148371830614483	28.29871227804987	29.482200208296348	21.0707156830393
56-57	21.216330263683474	28.141846993894724	29.41440977022738	21.227412972194422
58-59	21.270813374127375	27.53340269693942	29.73139630312583	21.464387625807383
60-61	21.42329437025563	27.96582757065402	29.465079940381873	21.145798118708477
62-63	20.86868235269475	27.91042334007907	29.928272674064143	21.29262163316204
64-65	21.05266636895457	27.873758738854466	29.756229763002974	21.31734512918799
66-67	21.126022770507948	27.81119385149585	29.58600200261796	21.476781375378238
68-69	21.27538330510856	28.000551818912058	29.49430878800805	21.229756087971335
70-71	21.41107942200023	27.842280599550882	29.419270287397975	21.32736969105091
72-73	21.206909606169756	27.77243818389939	29.562249449000266	21.458402760930586
74-75	21.17219136017087	27.720191507827973	29.49375934124329	21.61385779075787
76-77	21.336721379026166	27.893233637799604	29.168188998008375	21.601855985165855
78-79	21.2145946260561	27.942128607025353	29.37293743538993	21.470339331528614
80-81	21.035258098566644	28.033490008522822	29.42896426116225	21.502287631748292
82-83	20.991666633043497	28.016064950694552	29.62576209062752	21.36650632563443
84-85	21.030203557865164	27.633809256582285	29.430881159803985	21.905106025748562
86-87	21.20075420566492	27.90754112951604	29.228336760864543	21.663367903954498
88-89	20.806704016316385	27.795405462551525	29.483204890794724	21.914685630337363
90-91	20.90097188328129	27.75553432462091	29.243066625654656	22.100427166443133
92-93	20.76046897146149	27.944262207826437	29.58361913624708	21.711649684464998
94-95	20.90120553914461	27.438227171545737	29.533665901776416	22.12690138753323
96-97	20.82263925537368	27.936673144995083	29.226195018946942	22.01449258068429
98-99	20.341433920824162	27.748191431761764	29.55531314162869	22.355061505785383
100	20.645686571882	27.831680093073423	29.239388148007485	22.283245187037096
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3881.0
1	2402.0
2	1974.0
3	2698.0
4	1573.0
5	1162.5
6	2733.0
7	7660.0
8	13399.0
9	13744.5
10	8695.5
11	4734.0
12	4269.5
13	4521.0
14	4863.5
15	5372.0
16	6028.0
17	6829.5
18	7840.0
19	9047.0
20	10977.5
21	13510.0
22	17027.5
23	22271.5
24	28592.5
25	36719.0
26	48133.5
27	62425.0
28	79763.5
29	97720.0
30	111818.0
31	124402.0
32	138968.0
33	151411.0
34	163344.5
35	178171.0
36	189889.0
37	199268.5
38	211223.5
39	218453.5
40	221347.0
41	222799.5
42	219621.0
43	218603.5
44	215596.0
45	207061.0
46	200716.0
47	193542.5
48	180807.5
49	176864.0
50	178282.5
51	169888.5
52	156087.0
53	142226.0
54	132491.5
55	124397.5
56	108435.5
57	90618.0
58	81774.0
59	76803.5
60	65176.0
61	50974.0
62	41841.0
63	33450.0
64	25671.5
65	19742.0
66	15094.5
67	11312.5
68	8271.5
69	5847.0
70	4041.0
71	3032.0
72	2194.5
73	1402.5
74	1013.5
75	705.5
76	422.5
77	264.5
78	177.0
79	87.0
80	40.5
81	16.0
82	6.5
83	9.5
84	11.0
85	6.5
86	3.0
87	1.0
88	1.0
89	2.5
90	5.0
91	5.0
92	3.0
93	3.0
94	6.0
95	11.0
96	12.0
97	9.5
98	6.5
99	2.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00868756179800938
2	3.4338188924938264E-5
3	3.7772007817432087E-4
4	0.0027813933029199995
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	1.0301456677481478E-4
24-25	1.1159911400604937E-4
26-27	5.751646644927159E-4
28-29	0.0010988220455980244
30-31	0.0018113394657904936
32-33	0.00434378089900469
34-35	0.0053395883778279
36-37	0.0039918144625240735
38-39	0.0027213014723013575
40-41	0.0035282489120374064
42-43	5.408264755677777E-4
44-45	4.034737198680246E-4
46-47	0.0034595725341875303
48-49	0.0022834895635083946
50-51	9.700538371295059E-4
52-53	1.7169094462469132E-5
54-55	4.2922736156172827E-4
56-57	5.236573811053085E-4
58-59	6.695946840362962E-4
60-61	2.747055113995061E-4
62-63	0.0
64-65	0.0
66-67	7.382710618861727E-4
68-69	7.640247035798764E-4
70-71	0.0021633059022711106
72-73	1.0301456677481478E-4
74-75	0.001957276768721481
76-77	3.262127947869135E-4
78-79	9.614692898982714E-4
80-81	9.443001954358022E-4
82-83	0.0010730684039043207
84-85	9.528847426670368E-4
86-87	2.2319822801209873E-4
88-89	0.0015366339543909874
90-91	0.0018628467491779007
92-93	0.0031591133810943204
94-95	9.271311009733331E-4
96-97	0.0022062286384272834
98-99	0.0022233977328897526
100	0.003433818892493826
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5824419.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.16487917301759
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.3549185424129	27.55682243703247
2	17.056594805006352	14.383785181124246
3	6.938963920181256	8.777417258411132
4	3.4993619099361704	5.902006884604746
5	2.0386241183555325	4.297916981483028
6	1.296702475408376	3.2805181919368187
7	0.8788109831152111	2.5938471243281573
8	0.5663504072377453	1.9104077192615068
9	0.41974295323591126	1.5928569816225993
>10	1.746883464556245	12.440117566051418
>50	0.10798178262190047	3.157084751505198
>100	0.08112937352369441	6.829672645790848
>500	0.008678995319437329	2.497572373871249
>1k	0.005093284001144582	3.463440451982701
>5k	4.074627200700903E-5	0.09112598916953861
>10k+	1.2223881602102708E-4	1.2254074618245365
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31670	0.5437452216263974	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21920	0.37634655061732336	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17735	0.30449389029189006	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.7169094462469132E-5	0.0
5	0.0	0.0	0.0	1.7169094462469132E-5	0.0
6	0.0	0.0	0.0	1.7169094462469132E-5	0.0
7	0.0	0.0	0.0	1.7169094462469132E-5	0.0
8	0.0	0.0	0.0	1.7169094462469132E-5	0.0
9	0.0	0.0	0.0	5.15072833874074E-5	0.0
10-11	0.0	0.0	0.0	5.15072833874074E-5	0.0
12-13	0.0	0.0	0.0	6.867637784987653E-5	0.0
14-15	0.0	0.0	0.0	8.584547231234566E-5	0.0
16-17	8.584547231234566E-6	0.0	0.0	1.030145667748148E-4	0.0
18-19	1.7169094462469132E-5	0.0	0.0	1.1159911400604935E-4	0.0
20-21	1.7169094462469132E-5	0.0	0.0	1.802754918559259E-4	0.0
22-23	1.7169094462469132E-5	0.0	0.0	5.665801172614814E-4	0.0
24-25	1.7169094462469132E-5	0.0	0.0	9.700538371295059E-4	0.0
26-27	1.7169094462469132E-5	0.0	0.0	0.0016053103322408637	0.0
28-29	1.7169094462469132E-5	0.0	0.0	0.0036055098371185175	0.0
30-31	1.7169094462469132E-5	0.0	0.0	0.008163904416904072	0.0
32-33	1.7169094462469132E-5	0.0	0.0	0.01682571257321975	0.0
34-35	1.7169094462469132E-5	0.0	0.0	0.02876681777186703	0.0
36-37	1.7169094462469132E-5	0.0	0.0	0.04740386981087727	0.0
38-39	1.7169094462469132E-5	0.0	0.0	0.07515771100945862	0.0
40-41	1.7169094462469132E-5	0.0	0.0	0.10375283783670097	0.0
42-43	1.7169094462469132E-5	0.0	0.0	0.13071690069000874	0.0
44-45	1.7169094462469132E-5	0.0	0.0	0.1639648521165802	0.0
46-47	2.5753641693703696E-5	0.0	0.0	0.20341943119133427	0.0
48-49	3.4338188924938264E-5	0.0	0.0	0.24653102738659427	0.0
50-51	3.4338188924938264E-5	0.0	0.0	0.29458732278704536	0.0
52-53	3.4338188924938264E-5	0.0	0.0	0.3404288050018379	0.0
54-55	3.4338188924938264E-5	0.0	0.0	0.3913780928192151	0.0
56-57	6.009183061864196E-5	0.0	0.0	0.4510321115290641	0.0
58-59	6.867637784987653E-5	8.584547231234566E-6	0.0	0.5195625520760097	0.0
60-61	6.867637784987653E-5	1.7169094462469132E-5	0.0	0.595183828635955	0.0
62-63	6.867637784987653E-5	1.7169094462469132E-5	0.0	0.6743934459385563	0.0
64-65	6.867637784987653E-5	1.7169094462469132E-5	0.0	0.7552598808567859	0.0
66-67	7.72609250811111E-5	1.7169094462469132E-5	0.0	0.8482305273710562	0.0
68-69	8.584547231234566E-5	1.7169094462469132E-5	0.0	0.9589025102761323	0.0
70-71	8.584547231234566E-5	1.7169094462469132E-5	0.0	1.0831552469010215	0.0
72-73	8.584547231234566E-5	1.7169094462469132E-5	0.0	1.2145245731806038	0.0
74-75	8.584547231234566E-5	1.7169094462469132E-5	0.0	1.3527443681507116	0.0
76-77	8.584547231234566E-5	1.7169094462469132E-5	0.0	1.4955568958895298	0.0
78-79	8.584547231234566E-5	3.4338188924938264E-5	0.0	1.6602256808790714	0.0
80-81	8.584547231234566E-5	3.4338188924938264E-5	0.0	1.8513262867935842	0.0
82-83	8.584547231234566E-5	3.4338188924938264E-5	0.0	2.05872036335298	0.0
84-85	8.584547231234566E-5	3.4338188924938264E-5	0.0	2.2679257793781664	0.0
86-87	8.584547231234566E-5	4.292273615617283E-5	0.0	2.4920597230384693	0.0
88	8.584547231234566E-5	5.15072833874074E-5	0.0	2.6743439989465045	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10775	0.0	63.42784	1
GTATCAA	23970	0.0	48.886288	1
ATCAACG	32110	0.0	36.182617	3
TCAACGC	32320	0.0	35.918434	4
TATCAAC	34010	0.0	34.285614	2
CAACGCA	34090	0.0	34.135635	5
AACGCAG	35455	0.0	32.834682	6
ACGCAGA	38460	0.0	30.147007	7
CGCAGAG	38630	0.0	29.94134	8
GCAGAGT	42490	0.0	26.966917	9
TACATGG	26695	0.0	26.092161	2
GTACATG	27980	0.0	25.988153	1
GTGGTAT	3590	0.0	25.924091	1
ACATGGG	27415	0.0	24.721157	3
TGGTATC	3950	0.0	23.202206	2
AGAGTAC	40705	0.0	23.17309	10-11
AGTACTT	28150	0.0	22.530914	12-13
GAGTACT	26975	0.0	21.891996	12-13
CAGAGTA	41870	0.0	21.47879	10-11
CATGGGG	16010	0.0	21.400702	4
>>END_MODULE