FastQCFastQC Report
Wed 25 May 2016
SRR2431372_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431372_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4654982
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA175560.37714431548822314No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54300.053.8376851
GTATCAA106050.040.5957571
TCAACGC123650.033.5251164
ATCAACG124900.033.4153753
CAACGCA137600.030.1263145
TATCAAC143350.029.4752732
AACGCAG143500.028.8221666
GTACATG251050.026.8088761
ACGCAGA155700.026.1411747
TACATGG243550.026.090622
CGCAGAG159400.025.1510738
ACATGGG257350.024.1436613
GCAGAGT175450.022.3413039
CATGGGG165450.022.2428684
GTGGTAT26800.019.1155821
CATGGGA64750.018.7273254
CAGAGTA167550.018.69612110-11
AGAGTAC156100.018.44161810-11
ATGGGGA72900.017.6652135
GTATATA35300.017.574991
Produced by FastQC (version 0.11.5)