##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431372_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4654982
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.222222986039473	33.0	31.0	34.0	28.0	34.0
2	31.264696834488298	34.0	31.0	34.0	28.0	34.0
3	31.353833591622912	34.0	31.0	34.0	28.0	34.0
4	34.58195993024248	37.0	35.0	37.0	32.0	37.0
5	34.699072520581176	37.0	35.0	37.0	32.0	37.0
6	34.73741831869597	37.0	35.0	37.0	32.0	37.0
7	34.64401989094695	37.0	35.0	37.0	32.0	37.0
8	34.594435810922576	37.0	35.0	37.0	32.0	37.0
9	36.09288306592808	39.0	37.0	39.0	32.0	39.0
10-11	36.12013462135836	39.0	37.0	39.0	32.0	39.0
12-13	36.08047668068319	39.0	37.0	39.0	31.5	39.0
14-15	37.46458804781629	41.0	38.0	41.0	31.5	41.0
16-17	37.48012000905696	41.0	38.0	41.0	31.5	41.0
18-19	37.5078306425245	41.0	38.5	41.0	31.0	41.0
20-21	37.55841268559148	41.0	39.0	41.0	31.0	41.0
22-23	37.5666644897875	41.0	38.0	41.0	31.0	41.0
24-25	37.590290037641395	41.0	38.0	41.0	31.0	41.0
26-27	37.591801966151536	41.0	38.0	41.0	31.0	41.0
28-29	37.56569413157774	40.5	38.0	41.0	31.0	41.0
30-31	37.492943581736725	40.0	38.0	41.0	30.0	41.0
32-33	37.418508600033256	40.0	38.0	41.0	30.0	41.0
34-35	37.34933733363523	40.0	38.0	41.0	30.0	41.0
36-37	37.24745423290573	40.0	38.0	41.0	30.0	41.0
38-39	37.13749150909714	40.0	38.0	41.0	30.0	41.0
40-41	37.03885804499352	40.0	38.0	41.0	30.0	41.0
42-43	36.90446128470529	40.0	37.5	41.0	30.0	41.0
44-45	36.75609239734976	40.0	37.0	41.0	30.0	41.0
46-47	36.609400423030635	40.0	37.0	41.0	29.5	41.0
48-49	36.458833675404115	40.0	36.5	41.0	29.0	41.0
50-51	36.30130363554574	40.0	36.0	41.0	29.0	41.0
52-53	36.110001284645136	40.0	35.5	41.0	29.0	41.0
54-55	35.927846552360464	39.5	35.0	41.0	28.5	41.0
56-57	35.69746284733217	39.0	35.0	41.0	28.0	41.0
58-59	35.45001795925312	39.0	35.0	41.0	28.0	41.0
60-61	35.25347316058365	39.0	35.0	41.0	28.0	41.0
62-63	35.07734906386319	38.0	35.0	40.5	27.5	41.0
64-65	34.81779328040366	38.0	34.0	40.0	27.0	41.0
66-67	34.508111846619386	37.0	34.0	40.0	26.5	41.0
68-69	34.192255523222215	37.0	34.0	40.0	26.0	41.0
70-71	33.9466732631834	36.0	34.0	39.0	26.0	41.0
72-73	33.70493645303033	36.0	34.0	39.0	26.0	41.0
74-75	33.371441608152296	35.5	34.0	39.0	26.0	40.5
76-77	33.03220979157385	35.0	33.5	37.5	26.0	40.0
78-79	32.70794108333824	35.0	33.0	37.0	25.5	39.0
80-81	32.386159387941774	35.0	33.0	37.0	25.0	39.0
82-83	32.06372172867693	35.0	33.0	36.0	24.5	39.0
84-85	31.783014520786544	35.0	33.0	36.0	24.0	37.0
86-87	31.528802581835976	35.0	33.0	35.5	23.5	37.0
88-89	31.290087587878965	35.0	32.5	35.0	23.0	36.5
90-91	31.08824910601158	35.0	32.0	35.0	21.5	36.0
92-93	30.89056284213344	35.0	32.0	35.0	20.0	36.0
94-95	30.679921748354772	35.0	32.0	35.0	19.0	36.0
96-97	30.48978320431744	35.0	32.0	35.0	17.5	35.5
98-99	30.368592402720353	35.0	32.0	35.0	5.0	35.0
100	30.334765419071438	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4801229041875814
1101	2	0.5065737922359617
1101	3	0.4767106309849787
1101	4	0.487807334806341
1101	5	0.4001280153334861
1101	6	0.4195297018453914
1101	7	0.48801429149281716
1101	8	0.5067264261810465
1101	9	0.5320993742829287
1101	10-11	0.5244066316950438
1101	12-13	0.52991605585202
1101	14-15	0.620294187489506
1101	16-17	0.6719469923899268
1101	18-19	0.6070067593005817
1101	20-21	0.5930754772697142
1101	22-23	0.6036753573602596
1101	24-25	0.6180191869611562
1101	26-27	0.6382843251942845
1101	28-29	0.6834840309979953
1101	30-31	0.7140119904311533
1101	32-33	0.6803284748477054
1101	34-35	0.7292945588934998
1101	36-37	0.6747973511688414
1101	38-39	0.7027567033387214
1101	40-41	0.7004090744888742
1101	42-43	0.7085536078171017
1101	44-45	0.6484155362719477
1101	46-47	0.6500597694108663
1101	48-49	0.6551059734098104
1101	50-51	0.6464821333118564
1101	52-53	0.6200461240590656
1101	54-55	0.6501742070028627
1101	56-57	0.683840071645534
1101	58-59	0.6731311219535172
1101	60-61	0.6880122395356736
1101	62-63	0.7142883714738204
1101	64-65	0.6834778674471096
1101	66-67	0.6931175494163924
1101	68-69	0.6717531103548566
1101	70-71	0.6545665881537204
1101	72-73	0.6438269911722827
1101	74-75	0.673905549767511
1101	76-77	0.7015119514791337
1101	78-79	0.6180071262515057
1101	80-81	0.675653169012854
1101	82-83	0.6720104360650794
1101	84-85	0.6725422947177826
1101	86-87	0.6625406467899673
1101	88-89	0.6574262587153505
1101	90-91	0.6487973029729801
1101	92-93	0.6051245499890534
1101	94-95	0.6056413232562505
1101	96-97	0.6297457930800903
1101	98-99	0.6693410553116799
1101	100	0.7637240250452919
1102	1	0.31689317134241435
1102	2	0.4431993872999307
1102	3	0.44673734459849257
1102	4	0.39604943262349224
1102	5	0.40518543794257766
1102	6	0.4413366358922204
1102	7	0.38578613662922834
1102	8	0.44305678312447583
1102	9	0.5520870098029391
1102	10-11	0.490890561659306
1102	12-13	0.5055730307775903
1102	14-15	0.6194221994127815
1102	16-17	0.5756134405183886
1102	18-19	0.4991486875120188
1102	20-21	0.5319984785184602
1102	22-23	0.4841251967430438
1102	24-25	0.5544023581226156
1102	26-27	0.5186608347289408
1102	28-29	0.5502583087863187
1102	30-31	0.5518279889426125
1102	32-33	0.5964282311889235
1102	34-35	0.6195323217629252
1102	36-37	0.6324722041130997
1102	38-39	0.6054180085873853
1102	40-41	0.6144099391956246
1102	42-43	0.6315873765442532
1102	44-45	0.5902118080948995
1102	46-47	0.5800437121174511
1102	48-49	0.5373845081667881
1102	50-51	0.5433579078752899
1102	52-53	0.5718917337585054
1102	54-55	0.5389592467889202
1102	56-57	0.572865296818108
1102	58-59	0.5742785508137231
1102	60-61	0.5286931203361291
1102	62-63	0.5858105416870316
1102	64-65	0.6340442267532325
1102	66-67	0.6194683314653275
1102	68-69	0.6096466402367895
1102	70-71	0.6189474446404404
1102	72-73	0.6679626706278228
1102	74-75	0.7071327774426948
1102	76-77	0.6470604434917675
1102	78-79	0.6048280478254142
1102	80-81	0.6718456500983763
1102	82-83	0.629059734484656
1102	84-85	0.6450778189826529
1102	86-87	0.6172057288824924
1102	88-89	0.5773449479427164
1102	90-91	0.5693152995100199
1102	92-93	0.656180204613289
1102	94-95	0.6208111557749092
1102	96-97	0.7037523126226262
1102	98-99	0.7583646225064733
1102	100	0.7773006983507571
1103	1	0.277947211782795
1103	2	0.233200682543508
1103	3	0.29994892503551895
1103	4	0.2745872945121448
1103	5	0.2854741813164452
1103	6	0.2839930365542287
1103	7	0.265411043683514
1103	8	0.30672827097556876
1103	9	0.2723838124701814
1103	10-11	0.3202194335980977
1103	12-13	0.3371973141960751
1103	14-15	0.3653021735079065
1103	16-17	0.36215324527241677
1103	18-19	0.3057620812900552
1103	20-21	0.34064398954001973
1103	22-23	0.3818904458375698
1103	24-25	0.4319398122494107
1103	26-27	0.4437925418120017
1103	28-29	0.47157394045742507
1103	30-31	0.44009456925970625
1103	32-33	0.39507127165329337
1103	34-35	0.45702041271781013
1103	36-37	0.4475388371993816
1103	38-39	0.4257536205899797
1103	40-41	0.3624901483534799
1103	42-43	0.33380634466687553
1103	44-45	0.3180073754835391
1103	46-47	0.30195158192148597
1103	48-49	0.3451593996301696
1103	50-51	0.2743686056277994
1103	52-53	0.2911945329237895
1103	54-55	0.2744881619024895
1103	56-57	0.17717339688766742
1103	58-59	0.19554702602420093
1103	60-61	0.23753572425543723
1103	62-63	0.24758958272706622
1103	64-65	0.259846462247701
1103	66-67	0.2994625988131929
1103	68-69	0.37547204619970387
1103	70-71	0.29918255533579696
1103	72-73	0.2840792425498151
1103	74-75	0.33010058824118005
1103	76-77	0.3402248674681658
1103	78-79	0.3401792747089232
1103	80-81	0.3473429037022697
1103	82-83	0.33356974863639266
1103	84-85	0.3354705176466375
1103	86-87	0.4122287794209818
1103	88-89	0.400831263934176
1103	90-91	0.3087159135514206
1103	92-93	0.3048032887321632
1103	94-95	0.3316645773766922
1103	96-97	0.32799236443237234
1103	98-99	0.3493522697205016
1103	100	0.2607383707500759
1104	1	0.2505325664370943
1104	2	0.23290001559368534
1104	3	0.30481511033507047
1104	4	0.20065405837058137
1104	5	0.2662833142816794
1104	6	0.2595961727107152
1104	7	0.26777227564659256
1104	8	0.3111156299231439
1104	9	0.3887092518420303
1104	10-11	0.3183408391288438
1104	12-13	0.3149031215956626
1104	14-15	0.38722168035533144
1104	16-17	0.4471746198089406
1104	18-19	0.33496074279866406
1104	20-21	0.35495218693881014
1104	22-23	0.3304247288952098
1104	24-25	0.3460381443008771
1104	26-27	0.3758171764819096
1104	28-29	0.4255933175882731
1104	30-31	0.4259136088978792
1104	32-33	0.484193320027245
1104	34-35	0.4399427320042548
1104	36-37	0.45411077250179943
1104	38-39	0.489789803478871
1104	40-41	0.3992938860275288
1104	42-43	0.3679777886534197
1104	44-45	0.3930182808593159
1104	46-47	0.3825994415979963
1104	48-49	0.3033315553359799
1104	50-51	0.2843076388375465
1104	52-53	0.3928363200278966
1104	54-55	0.32619803298947403
1104	56-57	0.32542524219203983
1104	58-59	0.27210365936250014
1104	60-61	0.33767403885831015
1104	62-63	0.36509339960858256
1104	64-65	0.38284529135471956
1104	66-67	0.42752944302702645
1104	68-69	0.43354361990282086
1104	70-71	0.36168039147965203
1104	72-73	0.37913293143666493
1104	74-75	0.3874927914714732
1104	76-77	0.4236587521926012
1104	78-79	0.36719988012946914
1104	80-81	0.37620360187591473
1104	82-83	0.4565931664959493
1104	84-85	0.4052647389454087
1104	86-87	0.37054087979635497
1104	88-89	0.41275171208772576
1104	90-91	0.41196694382244914
1104	92-93	0.37519708759111836
1104	94-95	0.4363169254762873
1104	96-97	0.39408977813641144
1104	98-99	0.3447704277451358
1104	100	0.44303607602942563
1105	1	0.21139971878995922
1105	2	0.26212500518442994
1105	3	0.2568396132946269
1105	4	0.23894045552118115
1105	5	0.2303268751812908
1105	6	0.21266074131909818
1105	7	0.16351692970032872
1105	8	0.2354146615081163
1105	9	0.2379478112244584
1105	10-11	0.2614215513184277
1105	12-13	0.3156127610973485
1105	14-15	0.33049307976763487
1105	16-17	0.359521354895584
1105	18-19	0.3557623927195124
1105	20-21	0.4021388509539108
1105	22-23	0.3393644414775565
1105	24-25	0.376429379362456
1105	26-27	0.4258274219116558
1105	28-29	0.4416860550634638
1105	30-31	0.475967506039531
1105	32-33	0.47482306237269967
1105	34-35	0.45655762853842674
1105	36-37	0.4872763021635578
1105	38-39	0.5100725243582716
1105	40-41	0.4663979652329502
1105	42-43	0.44712587129409087
1105	44-45	0.4421820874112896
1105	46-47	0.3771677139675802
1105	48-49	0.40584049586187376
1105	50-51	0.4366056034478021
1105	52-53	0.408101104085425
1105	54-55	0.38111974084986144
1105	56-57	0.4004873177845809
1105	58-59	0.4458011524645542
1105	60-61	0.4745656214837126
1105	62-63	0.5063625506498255
1105	64-65	0.4790333199244401
1105	66-67	0.450533604730353
1105	68-69	0.4724926005441432
1105	70-71	0.49025324982349616
1105	72-73	0.5127351129951592
1105	74-75	0.4917726530185149
1105	76-77	0.4962734504641162
1105	78-79	0.4664832298630799
1105	80-81	0.4523021064428576
1105	82-83	0.5213875624015714
1105	84-85	0.518730561246759
1105	86-87	0.509511245942317
1105	88-89	0.5311090590135912
1105	90-91	0.5098772340123361
1105	92-93	0.5251723326437165
1105	94-95	0.5582326247139697
1105	96-97	0.6254286771075499
1105	98-99	0.6051492177117801
1105	100	0.6631052346554007
1106	1	0.17129404636414236
1106	2	0.18861525027665849
1106	3	0.15442535457942697
1106	4	0.14919085135353782
1106	5	0.18528944680317494
1106	6	0.19463284254469926
1106	7	0.14018138064743368
1106	8	0.1314078148829907
1106	9	0.16805110314410854
1106	10-11	0.20015689105214562
1106	12-13	0.1994936467352204
1106	14-15	0.24607638215324812
1106	16-17	0.17523872008521124
1106	18-19	0.20297698088160843
1106	20-21	0.2734932917864512
1106	22-23	0.23636142949992944
1106	24-25	0.2595333707926386
1106	26-27	0.3102448903484216
1106	28-29	0.31105530533148595
1106	30-31	0.26468429866746845
1106	32-33	0.2858159108636471
1106	34-35	0.2786686853522582
1106	36-37	0.30585903801625847
1106	38-39	0.2500440392421268
1106	40-41	0.26557992030718225
1106	42-43	0.3353961166205863
1106	44-45	0.29648018862172876
1106	46-47	0.28349051146538784
1106	48-49	0.29019356402772445
1106	50-51	0.2456225198551678
1106	52-53	0.2671302459939042
1106	54-55	0.3223030444756674
1106	56-57	0.25590059225866213
1106	58-59	0.3027085304489745
1106	60-61	0.2551802528463156
1106	62-63	0.24007511710419038
1106	64-65	0.2808280398515208
1106	66-67	0.31553735204668953
1106	68-69	0.2736909451105305
1106	70-71	0.2110929535658812
1106	72-73	0.1645440995954246
1106	74-75	0.20677077203968963
1106	76-77	0.2640988038194223
1106	78-79	0.25808018519224163
1106	80-81	0.26464042752800765
1106	82-83	0.22080434700262686
1106	84-85	0.2394516272449998
1106	86-87	0.2662615398021906
1106	88-89	0.24418699061899574
1106	90-91	0.18664439381009856
1106	92-93	0.18817292794251372
1106	94-95	0.13084833333312673
1106	96-97	0.1470009161682455
1106	98-99	0.17599543684098862
1106	100	0.1334408894702932
1107	1	-0.0020643078966564588
1107	2	0.044569854879583204
1107	3	0.008164793811342719
1107	4	-0.0025498147161187035
1107	5	0.025265825375051065
1107	6	-0.05071467244864891
1107	7	0.007810225619316213
1107	8	-0.02482237804984777
1107	9	-0.028336417923412682
1107	10-11	-0.045763078192884166
1107	12-13	-0.05150117071565319
1107	14-15	0.01692815956742777
1107	16-17	0.034848516360519
1107	18-19	0.015272870555044449
1107	20-21	0.01995510436118053
1107	22-23	0.05001436586762509
1107	24-25	-0.004636672578840262
1107	26-27	0.019495450948440407
1107	28-29	0.017050379373905855
1107	30-31	0.047154421507293875
1107	32-33	0.03814076537173605
1107	34-35	0.10721724897975804
1107	36-37	0.04865399225592881
1107	38-39	0.08358312098649634
1107	40-41	0.06339740658629012
1107	42-43	0.06644067832019829
1107	44-45	0.06032341538503516
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2216	92-93	-1.2093342349187175
2216	94-95	-1.2672910721285646
2216	96-97	-1.3939043411922398
2216	98-99	-1.452047192533147
2216	100	-1.448303734608377
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	85022.0
3	18412.0
4	12685.0
5	11402.0
6	8447.0
7	8052.0
8	8915.0
9	9557.0
10	9386.0
11	10022.0
12	11400.0
13	12556.0
14	12589.0
15	12083.0
16	11876.0
17	11567.0
18	11712.0
19	11407.0
20	11173.0
21	12179.0
22	15143.0
23	24686.0
24	40541.0
25	50845.0
26	45987.0
27	34709.0
28	36208.0
29	43762.0
30	52381.0
31	63214.0
32	82472.0
33	111612.0
34	164313.0
35	267221.0
36	498570.0
37	939408.0
38	1312720.0
39	566953.0
40	3795.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.93129618319528	21.545029212881637	13.883127420667	22.64054718325608
2	14.934607667487294	22.649205796802562	39.45172696767762	22.964459568032517
3	16.98187017694161	29.369995415664334	30.34174997883988	23.306384428554182
4	11.85822750220785	19.368014334758545	39.54707421880958	29.226683944224025
5	12.335171220855418	40.56080130922096	34.450294329816955	12.653733140106665
6	27.3425117433322	40.75807382284185	19.018548299434883	12.880866134391066
7	24.87414559282936	35.325034554376366	23.486879218867013	16.313940633927263
8	23.960049684402644	37.01466514800701	21.27146785959645	17.753817307993888
9	25.38390051776784	18.45575342718833	21.16770805988079	34.99263799516304
10-11	22.822848724227075	28.987566439569477	28.77152908432299	19.41805575188046
12-13	23.522690313303038	27.390814830218464	28.273707610469817	20.812787246008686
14-15	21.59059906139272	28.044813062649865	26.08266798883433	24.281919887123088
16-17	21.135559707857087	31.17321398879738	26.274333606445737	21.416892696899794
18-19	21.131746588923438	30.208419710323263	27.995682904896302	20.664150795856997
20-21	22.961334759189185	28.9660840793799	27.26672197658337	20.80585918484755
22-23	22.479152443554025	29.15830823835624	27.266442705900907	21.096096612188834
24-25	21.917775407079983	29.186020483000796	27.26924615390564	21.62695795601358
26-27	21.762898331293226	29.47437820382549	27.183520795569134	21.579202669312146
28-29	21.75680808217948	29.176471573896528	27.526798170218488	21.539922173705506
30-31	22.055133618132146	29.256010012498436	27.14243578170657	21.54642058766285
32-33	21.510244293103604	29.229114097541085	27.62212614355974	21.63851546579557
34-35	22.09774387956817	28.880595027005473	27.347742698038356	21.673918395387997
36-37	22.253909896966302	29.089790250531582	27.046033690355838	21.610266162146278
38-39	21.93808697863923	29.052443167342002	27.284595300261095	21.724874553757672
40-41	21.86875266112737	28.9207348170197	27.36849465798149	21.842017863871437
42-43	21.72827950784772	29.04836151890598	27.541717669370154	21.681641303876148
44-45	21.863607635861964	28.855761418626326	27.353424782308505	21.92720616320321
46-47	22.052222758326458	28.84492356791068	27.390557041896184	21.712296631866675
48-49	21.866765542809834	28.76938084830403	27.49232972329431	21.871523885591824
50-51	22.040890813326453	28.858231890048124	26.963165485924545	22.137711810700882
52-53	21.73446642758232	28.894386702247182	27.348816816047837	22.02233005412266
54-55	21.921857055516003	28.98651380392019	27.267302000308486	21.824327140255324
56-57	21.87245836826007	28.864118056740068	27.235626260208956	22.027797314790906
58-59	22.018849911771945	28.512731091119146	27.303811271450673	22.164607725658232
60-61	22.22963482995208	28.914494191384627	27.019288151919813	21.836582826743474
62-63	21.696410426506482	28.780981322806404	27.436421880900934	22.08618636978618
64-65	22.01850855905657	28.549103793430756	27.351548013670946	22.080839633841723
66-67	21.85953672860604	28.665191401384583	27.21228567586298	22.2629861941464
68-69	21.990095772658197	28.78234545267844	27.17189883870657	22.055659935956786
70-71	22.160074947658227	28.67988533575425	27.135045849801354	22.024993866786165
72-73	21.94142748564871	28.642119346540973	27.37934324987723	22.037109917933087
74-75	21.902974060909365	28.588295293085984	27.31684032290565	22.191890323098995
76-77	21.924563832899892	28.691034680692646	27.13333800216628	22.251063484241186
78-79	21.914606758952022	28.738886638014925	27.327044443995703	22.01946215903735
80-81	21.851448619994667	28.787737525086026	27.294154950545458	22.066658904373853
82-83	21.704317765694654	28.811248691454328	27.526870702046434	21.957562840804584
84-85	21.895604419211555	28.438867630744745	27.306932322337435	22.35859562770626
86-87	21.949937888265474	28.74194870263544	27.23904344169365	22.06906996740544
88-89	21.710500027283512	28.709418998690822	27.30687462861125	22.273206345414415
90-91	21.771876590077284	28.540738578914937	27.207332060179457	22.480052770828326
92-93	21.685913686431853	28.614164092215983	27.588239988751585	22.111682232600586
94-95	21.853446673909247	28.303066524393007	27.480714321467865	22.362772480229882
96-97	21.794404268631627	28.816528863479512	27.10950586883142	22.279560999057445
98-99	21.407201998261165	28.672381713083155	27.44503739865134	22.475378890004333
100	21.515026848427496	28.69904088974153	27.228892108957677	22.557040152873302
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	13904.0
1	9927.5
2	4865.0
3	3241.5
4	2356.5
5	1847.5
6	1666.0
7	1748.0
8	1935.0
9	1969.5
10	1741.5
11	1541.5
12	1603.0
13	1676.5
14	1738.5
15	1838.0
16	2123.0
17	2895.0
18	3743.0
19	4816.0
20	7036.5
21	9248.5
22	11303.5
23	14799.0
24	20605.0
25	26647.0
26	33108.5
27	42708.5
28	54514.5
29	68313.5
30	80077.0
31	90266.5
32	100246.5
33	107089.5
34	114280.5
35	124249.0
36	133748.5
37	141019.5
38	149321.5
39	156548.5
40	162831.0
41	166676.0
42	164290.5
43	164062.5
44	163869.5
45	161233.5
46	160369.5
47	156114.5
48	148616.0
49	148588.0
50	150254.5
51	144602.0
52	136080.5
53	127739.5
54	121232.0
55	114909.5
56	102054.5
57	88063.5
58	80683.0
59	75780.0
60	68291.5
61	57887.5
62	49396.5
63	42056.0
64	34620.5
65	27512.0
66	21599.5
67	16726.0
68	12829.0
69	9839.0
70	7521.5
71	5916.0
72	4476.0
73	3227.0
74	2442.0
75	1869.0
76	1273.5
77	871.5
78	635.5
79	467.5
80	337.5
81	249.0
82	198.0
83	171.5
84	161.5
85	142.0
86	122.0
87	102.5
88	92.0
89	83.5
90	63.0
91	46.0
92	43.5
93	47.5
94	40.5
95	32.0
96	29.0
97	26.0
98	22.0
99	21.0
100	50.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	3.22235402843663E-4
2	4.296472037915507E-5
3	0.0
4	6.44470805687326E-5
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	1.0741180094788767E-5
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	1.0741180094788766E-4
84-85	7.304002464456361E-4
86-87	0.0013856122322277507
88-89	0.003351248189574095
90-91	0.002953824526066911
92-93	0.0022663890000004295
94-95	0.0022126830995264856
96-97	0.0020300830379150767
98-99	0.002900118625592967
100	0.0031579069478678973
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4654982.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.870047302065025
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.51025392839131	34.23199238134774
2	14.833519098182144	14.201625217721284
3	5.590464403458411	8.028473863021945
4	2.6423944445807117	5.0596618821117
5	1.4540280450898588	3.4802195648490337
6	0.899067904398408	2.582305386679216
7	0.5932294827108056	1.9878546378842077
8	0.40641939335720656	1.556425246758884
9	0.2947843472858685	1.27002065836346
>10	1.5699644332352798	13.568221066009231
>50	0.11977068643198538	3.9883083926790186
>100	0.0798063671256963	7.183950639078448
>500	0.004858045374016173	1.6105715006708956
>1k	0.0013944384915338028	0.8723388394498311
>5k	0.0	0.0
>10k+	4.498188682362193E-5	0.37803072337500093
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	17556	0.37714431548822314	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	2.148236018957753E-5	0.0	0.0	0.0	0.0
4	2.148236018957753E-5	0.0	0.0	0.0	0.0
5	6.44470805687326E-5	0.0	0.0	0.0	0.0
6	6.44470805687326E-5	0.0	0.0	0.0	0.0
7	6.44470805687326E-5	0.0	0.0	2.148236018957753E-5	0.0
8	6.44470805687326E-5	0.0	0.0	2.148236018957753E-5	0.0
9	1.7185888151662024E-4	0.0	0.0	2.148236018957753E-5	0.0
10-11	1.7185888151662024E-4	0.0	0.0	2.148236018957753E-5	0.0
12-13	1.7185888151662024E-4	0.0	0.0	5.3705900473943825E-5	0.0
14-15	1.7185888151662024E-4	0.0	0.0	8.592944075831012E-5	0.0
16-17	1.7185888151662024E-4	0.0	0.0	8.592944075831012E-5	0.0
18-19	1.7185888151662024E-4	0.0	0.0	8.592944075831012E-5	0.0
20-21	1.7185888151662024E-4	0.0	0.0	1.3963534123225395E-4	0.0
22-23	1.7185888151662024E-4	0.0	0.0	4.0816484360197314E-4	0.0
24-25	1.7185888151662024E-4	0.0	0.0	7.733649668247911E-4	0.0
26-27	1.9334124170619779E-4	0.0	0.0	0.001149306270142398	0.0
28-29	2.0408242180098657E-4	0.0	0.0	0.002728259744076347	0.0
30-31	2.1482360189577533E-4	0.0	0.0	0.006960284701423121	0.0
32-33	2.1482360189577533E-4	0.0	0.0	0.01418909890521596	0.0
34-35	2.577883222749304E-4	0.0	0.0	0.025005467260668247	0.0
36-37	2.577883222749304E-4	0.0	0.0	0.04046202541706928	0.0
38-39	2.577883222749304E-4	0.0	0.0	0.06440411584835344	0.0
40-41	2.577883222749304E-4	0.0	0.0	0.09235266645499382	0.0
42-43	2.577883222749304E-4	0.0	0.0	0.11844299290523572	0.0
44-45	2.6852950236971916E-4	0.0	0.0	0.1487760854929192	0.0
46-47	2.900118625592967E-4	0.0	0.0	0.1851886860142531	0.0
48-49	3.0075304265408546E-4	0.0	0.0	0.22479141702373928	0.0
50-51	3.0075304265408546E-4	0.0	0.0	0.2699688205024209	0.0
52-53	3.0075304265408546E-4	0.0	0.0	0.31393247063039126	0.0
54-55	3.0075304265408546E-4	0.0	0.0	0.3606458628626276	0.0
56-57	3.3297658293845176E-4	0.0	0.0	0.4150714224029223	0.0
58-59	3.6520012322281806E-4	0.0	0.0	0.47855179676312387	0.0
60-61	4.2964720379155065E-4	0.0	0.0	0.550195467995365	0.0
62-63	5.370590047394383E-4	0.0	0.0	0.6260174582844789	0.0
64-65	5.370590047394383E-4	0.0	0.0	0.7068770620380487	0.0
66-67	5.370590047394383E-4	0.0	0.0	0.7944499033508615	0.0
68-69	5.585413649290158E-4	0.0	0.0	0.8943213958722074	0.0
70-71	5.585413649290158E-4	0.0	0.0	1.0139781421281544	0.0
72-73	5.585413649290158E-4	3.22235402843663E-5	0.0	1.143452326990738	0.0
74-75	5.800237251185934E-4	4.296472037915506E-5	0.0	1.2723357469481085	0.0
76-77	5.800237251185934E-4	4.296472037915506E-5	0.0	1.4109184525310732	0.0
78-79	5.907649052133821E-4	4.296472037915506E-5	0.0	1.570081259175653	0.0
80-81	6.015060853081709E-4	4.296472037915506E-5	0.0	1.7536265446354036	0.0
82-83	6.015060853081709E-4	4.296472037915506E-5	0.0	1.9537132474411287	0.0
84-85	6.015060853081709E-4	4.296472037915506E-5	0.0	2.1604272583653383	0.0
86-87	6.015060853081709E-4	4.296472037915506E-5	0.0	2.3744882364743836	0.0
88	6.015060853081709E-4	4.296472037915506E-5	0.0	2.545724129545506	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5430	0.0	53.837685	1
GTATCAA	10605	0.0	40.595757	1
TCAACGC	12365	0.0	33.525116	4
ATCAACG	12490	0.0	33.415375	3
CAACGCA	13760	0.0	30.126314	5
TATCAAC	14335	0.0	29.475273	2
AACGCAG	14350	0.0	28.822166	6
GTACATG	25105	0.0	26.808876	1
ACGCAGA	15570	0.0	26.141174	7
TACATGG	24355	0.0	26.09062	2
CGCAGAG	15940	0.0	25.151073	8
ACATGGG	25735	0.0	24.143661	3
GCAGAGT	17545	0.0	22.341303	9
CATGGGG	16545	0.0	22.242868	4
GTGGTAT	2680	0.0	19.115582	1
CATGGGA	6475	0.0	18.727325	4
CAGAGTA	16755	0.0	18.696121	10-11
AGAGTAC	15610	0.0	18.441618	10-11
ATGGGGA	7290	0.0	17.665213	5
GTATATA	3530	0.0	17.57499	1
>>END_MODULE