FastQCFastQC Report
Wed 25 May 2016
SRR2431372_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431372_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4654982
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT297910.6399809924077042No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189890.40792853763988773No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155840.33478110119437626No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92550.064.196371
GTATCAA215050.050.906111
ATCAACG287650.037.8250773
TCAACGC290100.037.5380334
TATCAAC296000.036.8374442
CAACGCA303750.035.8348965
AACGCAG312250.034.9196176
ACGCAGA342550.031.6799077
CGCAGAG346700.031.124478
GCAGAGT369650.028.9123679
GTGGTAT29500.027.0873661
GTACATG226850.024.9268131
TACATGG221500.024.7410452
AGAGTAC353500.024.00446310-11
TGGTATC33450.023.4646242
ACATGGG228750.023.2583313
AGTACTT251250.023.19527412-13
GAGTACT246600.022.27949512-13
CAGAGTA364950.022.11807410-11
CATGGGG148150.020.6207734
Produced by FastQC (version 0.11.5)