##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431372_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4654982
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.7938990956356	34.0	31.0	34.0	31.0	34.0
2	32.90788213574188	34.0	31.0	34.0	31.0	34.0
3	32.98675848800275	34.0	31.0	34.0	31.0	34.0
4	36.37293935830471	37.0	37.0	37.0	35.0	37.0
5	36.334461228851154	37.0	37.0	37.0	35.0	37.0
6	36.366507110016755	37.0	37.0	37.0	35.0	37.0
7	36.23725505275853	37.0	37.0	37.0	35.0	37.0
8	36.28750422665437	37.0	37.0	37.0	35.0	37.0
9	38.10965133699765	39.0	39.0	39.0	37.0	39.0
10-11	38.09951198522357	39.0	39.0	39.0	36.0	39.0
12-13	38.07851995990532	39.0	39.0	39.0	35.0	39.0
14-15	39.557837173162	41.0	39.5	41.0	37.0	41.0
16-17	39.575389228143095	41.0	39.5	41.0	37.0	41.0
18-19	39.656009410992354	41.0	40.0	41.0	37.0	41.0
20-21	39.66063381125856	41.0	40.0	41.0	37.0	41.0
22-23	39.58832397203685	41.0	40.0	41.0	37.0	41.0
24-25	39.56126393184764	41.0	40.0	41.0	37.0	41.0
26-27	39.47175531505815	41.0	40.0	41.0	36.0	41.0
28-29	39.38657206408102	41.0	39.0	41.0	36.0	41.0
30-31	39.21411865824615	41.0	39.0	41.0	35.5	41.0
32-33	39.01249457033346	41.0	39.0	41.0	35.0	41.0
34-35	38.912422969626945	41.0	39.0	41.0	35.0	41.0
36-37	38.79897902934963	41.0	38.5	41.0	35.0	41.0
38-39	38.67210421006998	41.0	38.0	41.0	35.0	41.0
40-41	38.56727995940693	41.0	38.0	41.0	35.0	41.0
42-43	38.42074652490601	41.0	38.0	41.0	35.0	41.0
44-45	38.19393963284928	41.0	38.0	41.0	34.5	41.0
46-47	37.859446717516846	40.0	38.0	41.0	33.5	41.0
48-49	37.563925918510535	40.0	38.0	41.0	33.0	41.0
50-51	37.392899263627655	40.0	37.5	41.0	33.0	41.0
52-53	37.26702971139308	40.0	37.0	41.0	33.0	41.0
54-55	37.11016444317078	40.0	37.0	41.0	33.0	41.0
56-57	36.927879635195154	40.0	36.0	41.0	32.0	41.0
58-59	36.725333309559524	40.0	36.0	41.0	32.0	41.0
60-61	36.514735824972036	39.0	35.0	41.0	31.5	41.0
62-63	36.27421588311191	39.0	35.0	41.0	31.0	41.0
64-65	35.99715369468669	39.0	35.0	41.0	31.0	41.0
66-67	35.69816499827497	38.0	35.0	41.0	31.0	41.0
68-69	35.41973996891932	37.0	35.0	40.0	30.5	41.0
70-71	35.15848321217999	37.0	35.0	40.0	30.5	41.0
72-73	34.896338159846806	36.0	35.0	39.0	30.5	41.0
74-75	34.597812623120774	36.0	35.0	39.0	30.0	41.0
76-77	34.285473606557446	35.5	35.0	38.5	30.0	40.5
78-79	33.977624188450136	35.0	35.0	37.0	30.0	39.5
80-81	33.68423315063302	35.0	35.0	37.0	30.0	39.0
82-83	33.37495794827993	35.0	34.5	36.5	29.0	39.0
84-85	33.127890183034005	35.0	34.0	36.0	29.0	37.5
86-87	32.919475735888994	35.0	34.0	36.0	29.0	37.0
88-89	32.709923797771935	35.0	34.0	35.5	29.0	37.0
90-91	32.524665831146066	35.0	34.0	35.0	29.0	36.0
92-93	32.387083666488934	35.0	34.0	35.0	29.0	36.0
94-95	32.261992957223036	35.0	34.0	35.0	29.0	36.0
96-97	32.13285518612102	35.0	34.0	35.0	29.0	36.0
98-99	32.01845861058109	35.0	34.0	35.0	29.0	36.0
100	31.9180832493015	35.0	34.0	35.0	27.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2653490675407255
1101	2	0.22120438610191684
1101	3	0.259107691313325
1101	4	0.14117291836455337
1101	5	0.19515519669776893
1101	6	0.20536795297932287
1101	7	0.18805546402663964
1101	8	0.22912440790578614
1101	9	0.28192378089435266
1101	10-11	0.30707110695379214
1101	12-13	0.2819065903694664
1101	14-15	0.36298281965527934
1101	16-17	0.36433830874727136
1101	18-19	0.38518806338370837
1101	20-21	0.406522029757312
1101	22-23	0.4164715592184507
1101	24-25	0.4313696895912216
1101	26-27	0.4151933982789515
1101	28-29	0.4482449492898013
1101	30-31	0.4805069420157011
1101	32-33	0.43856142198505665
1101	34-35	0.4787250949604669
1101	36-37	0.44418058024191964
1101	38-39	0.5099085476678198
1101	40-41	0.48144025593634154
1101	42-43	0.51132020928587
1101	44-45	0.5369351267516578
1101	46-47	0.5615812188783522
1101	48-49	0.49407692818901694
1101	50-51	0.5472159062483826
1101	52-53	0.5308496087849193
1101	54-55	0.496044145526632
1101	56-57	0.4731506976867621
1101	58-59	0.5076435826396022
1101	60-61	0.4782449667396733
1101	62-63	0.4411511515430533
1101	64-65	0.46894315409925014
1101	66-67	0.47149093136298603
1101	68-69	0.4542664282079869
1101	70-71	0.42155107635134215
1101	72-73	0.4089697563060142
1101	74-75	0.38774993824088
1101	76-77	0.40955074107120737
1101	78-79	0.4212881790342351
1101	80-81	0.4113181825686141
1101	82-83	0.42929405497185513
1101	84-85	0.39284083290454674
1101	86-87	0.4162053856186674
1101	88-89	0.42288757037762537
1101	90-91	0.45410690582922086
1101	92-93	0.46525108322288844
1101	94-95	0.44663938488635324
1101	96-97	0.46862065760092975
1101	98-99	0.5151531740822222
1101	100	0.5835922858467768
1102	1	0.13471488267590814
1102	2	0.17425212808868906
1102	3	0.2080822689545485
1102	4	0.08362716629549993
1102	5	0.12651821285159315
1102	6	0.16085607098561638
1102	7	0.17983233701757229
1102	8	0.20596723052980792
1102	9	0.23949342358685755
1102	10-11	0.2716364565170011
1102	12-13	0.26650272223351124
1102	14-15	0.3273098041636686
1102	16-17	0.323805179842104
1102	18-19	0.3116455316290043
1102	20-21	0.34313562047177015
1102	22-23	0.29712079871831065
1102	24-25	0.318660212462305
1102	26-27	0.3392687903169005
1102	28-29	0.311791065621982
1102	30-31	0.314868467563862
1102	32-33	0.323635789422255
1102	34-35	0.3389346417047605
1102	36-37	0.24919548088674048
1102	38-39	0.2534046993390362
1102	40-41	0.27469669944313324
1102	42-43	0.34312662872001454
1102	44-45	0.33728884671754145
1102	46-47	0.32099430163170695
1102	48-49	0.35745874728682026
1102	50-51	0.3477818424503809
1102	52-53	0.3435711296218571
1102	54-55	0.33491588721901167
1102	56-57	0.34265141415947653
1102	58-59	0.36477396453632593
1102	60-61	0.366808565635786
1102	62-63	0.3387257120829972
1102	64-65	0.3622502175864142
1102	66-67	0.407963126583617
1102	68-69	0.4200151409628958
1102	70-71	0.401434443272251
1102	72-73	0.3952813047952688
1102	74-75	0.40887036125054266
1102	76-77	0.397831421064474
1102	78-79	0.38730027410513657
1102	80-81	0.41690633026107804
1102	82-83	0.4321712122002026
1102	84-85	0.40008533483867126
1102	86-87	0.3867744104807542
1102	88-89	0.3866247920181607
1102	90-91	0.3707801158503372
1102	92-93	0.43981839284055013
1102	94-95	0.41433017016071716
1102	96-97	0.4322612540675408
1102	98-99	0.47144646907247534
1102	100	0.4697278820590327
1103	1	0.14911060357489703
1103	2	0.15600886215599985
1103	3	0.15322736022125127
1103	4	0.12571553069069097
1103	5	0.10393962274821433
1103	6	0.15092894042986416
1103	7	0.11406682407712765
1103	8	0.15275622575659753
1103	9	0.24091967865389563
1103	10-11	0.20347594225732735
1103	12-13	0.26345179997210977
1103	14-15	0.309578591192043
1103	16-17	0.3000009055382975
1103	18-19	0.28739516584262503
1103	20-21	0.30404269873805845
1103	22-23	0.2860979640537522
1103	24-25	0.31343282525643446
1103	26-27	0.3172443485908687
1103	28-29	0.32283834201256667
1103	30-31	0.3541837874170639
1103	32-33	0.33854816859282266
1103	34-35	0.3730291704999473
1103	36-37	0.31471139559255334
1103	38-39	0.31388842484334134
1103	40-41	0.3569835479236261
1103	42-43	0.3901206801939452
1103	44-45	0.3656738698640183
1103	46-47	0.31399586072119945
1103	48-49	0.29093405370787195
1103	50-51	0.3369900888344972
1103	52-53	0.3259891335075906
1103	54-55	0.3100801432934617
1103	56-57	0.3453417310144289
1103	58-59	0.2916005697988595
1103	60-61	0.2740648079902499
1103	62-63	0.27457018690969903
1103	64-65	0.35477874892411165
1103	66-67	0.3328235041231338
1103	68-69	0.35621295344041215
1103	70-71	0.3023030000351028
1103	72-73	0.34783770785588075
1103	74-75	0.3270364882323875
1103	76-77	0.29239250139356443
1103	78-79	0.25702553855070676
1103	80-81	0.27978673610406446
1103	82-83	0.2777830468887501
1103	84-85	0.24692287771362942
1103	86-87	0.22450339275511055
1103	88-89	0.19754851656302463
1103	90-91	0.23658220742885305
1103	92-93	0.24110473761519557
1103	94-95	0.2541424797719074
1103	96-97	0.23883140110167034
1103	98-99	0.2758854846873575
1103	100	0.28513710707162687
1104	1	0.11550257721832935
1104	2	0.16505389561827855
1104	3	0.1678004778959945
1104	4	0.08334231429360273
1104	5	0.1178586933806045
1104	6	0.12694051160752906
1104	7	0.10699772991208079
1104	8	0.15949528881262864
1104	9	0.20633051285774684
1104	10-11	0.24848920089840476
1104	12-13	0.24617341442689877
1104	14-15	0.2856272406473508
1104	16-17	0.2858650360354602
1104	18-19	0.2809896465745467
1104	20-21	0.27846487453271607
1104	22-23	0.2824759902955307
1104	24-25	0.312930087433152
1104	26-27	0.32599049440147354
1104	28-29	0.2947955011047796
1104	30-31	0.3013347866938645
1104	32-33	0.3407445571915346
1104	34-35	0.34019589647655124
1104	36-37	0.3953155304518816
1104	38-39	0.3286162955400229
1104	40-41	0.34261022821244325
1104	42-43	0.32941278355395553
1104	44-45	0.3778281981502687
1104	46-47	0.36901630543094655
1104	48-49	0.34076683411952047
1104	50-51	0.3753037389211613
1104	52-53	0.35750134178879023
1104	54-55	0.3232114899351757
1104	56-57	0.3096006131560145
1104	58-59	0.3266262950063492
1104	60-61	0.32237493391863836
1104	62-63	0.3320976971550138
1104	64-65	0.34712660745270085
1104	66-67	0.352538449079681
1104	68-69	0.35291047275440235
1104	70-71	0.3405415752190635
1104	72-73	0.3278711471818099
1104	74-75	0.35677714691707507
1104	76-77	0.3195477135892233
1104	78-79	0.286109165563893
1104	80-81	0.3180706776512423
1104	82-83	0.3183794102329074
1104	84-85	0.25390780533637525
1104	86-87	0.26833741293579294
1104	88-89	0.32434184247305353
1104	90-91	0.3277377754841382
1104	92-93	0.3423089551257874
1104	94-95	0.3427292004680069
1104	96-97	0.3702045830607332
1104	98-99	0.38879824168115107
1104	100	0.38338170891061196
1105	1	-0.004116428502335623
1105	2	0.04722032276016819
1105	3	0.08215168286217533
1105	4	-0.08154887292188562
1105	5	-0.09876856784661925
1105	6	-0.06700565938327685
1105	7	-0.3956092892831933
1105	8	-0.3669198876037285
1105	9	-0.30349078537599894
1105	10-11	-0.30741474600178265
1105	12-13	-0.20437255381175845
1105	14-15	-0.19711029077618747
1105	16-17	-0.14886113121038136
1105	18-19	-0.16336409918385186
1105	20-21	-0.1358099951267775
1105	22-23	-0.09448036045574781
1105	24-25	-0.11902279604534272
1105	26-27	-0.14099576352374044
1105	28-29	-0.1136804994698366
1105	30-31	-0.14215535926621214
1105	32-33	-0.1913029681247167
1105	34-35	-0.20479238041876613
1105	36-37	-0.21392578908510984
1105	38-39	-0.2122925156736315
1105	40-41	-0.2323939045833825
1105	42-43	-0.12641902706235442
1105	44-45	-0.17999726062492982
1105	46-47	-0.1923535631342972
1105	48-49	-0.20819363237123412
1105	50-51	-0.20650134685531896
1105	52-53	-0.20120457561729665
1105	54-55	-0.23831070068037974
1105	56-57	-0.22669252622630154
1105	58-59	-0.21088712873742566
1105	60-61	-0.16982183168586573
1105	62-63	-0.15272099963654284
1105	64-65	-0.16664666371992354
1105	66-67	-0.20757948559969464
1105	68-69	-0.20555378589313023
1105	70-71	-0.17100157797797522
1105	72-73	-0.15400252883050314
1105	74-75	-0.130716236775406
1105	76-77	-0.1397987162956298
1105	78-79	-0.16884988966480563
1105	80-81	-0.1725449362721463
1105	82-83	-0.160495682479052
1105	84-85	-0.11865918394939712
1105	86-87	-0.1303670525890226
1105	88-89	-0.12138359181442837
1105	90-91	-0.12833682091122967
1105	92-93	-0.12858040719950736
1105	94-95	-0.16719510976407292
1105	96-97	-0.10653951138665718
1105	98-99	-0.12464612540295406
1105	100	-0.1553630486431068
1106	1	0.08113204673350793
1106	2	0.07184318822815783
1106	3	0.05535738064330786
1106	4	0.07387695002357475
1106	5	0.0791313347972249
1106	6	0.09081440673347174
1106	7	0.06265089487359177
1106	8	0.09088292759458483
1106	9	0.13629799723796765
1106	10-11	0.14714701952077291
1106	12-13	0.11906569547925727
1106	14-15	0.12016542747523573
1106	16-17	0.20826358423128966
1106	18-19	0.1622799939569859
1106	20-21	0.14602223413628934
1106	22-23	0.2062288779407453
1106	24-25	0.128677677196535
1106	26-27	0.13232684518718685
1106	28-29	0.16099384745844958
1106	30-31	0.16541570538847594
1106	32-33	0.1472284783273352
1106	34-35	0.1689675214189421
1106	36-37	0.18987919681787702
1106	38-39	0.15335030367247526
1106	40-41	0.16236033485350987
1106	42-43	0.1768282193362296
1106	44-45	0.170974031538627
1106	46-47	0.14201632532297026
1106	48-49	0.17347060067859843
1106	50-51	0.169144658269488
1106	52-53	0.10071722234353331
1106	54-55	0.15874368992382415
1106	56-57	0.16103999513968859
1106	58-59	0.11262116203847938
1106	60-61	0.11842193869141227
1106	62-63	0.13415055620853877
1106	64-65	0.14177211175597648
1106	66-67	0.12183916103259662
1106	68-69	0.15668576657860456
1106	70-71	0.12281827403101886
1106	72-73	0.16253729090557556
1106	74-75	0.152852689730004
1106	76-77	0.14937858886803923
1106	78-79	0.14710365291421112
1106	80-81	0.14626827116192231
1106	82-83	0.17048080046260594
1106	84-85	0.17814411665168706
1106	86-87	0.13814911263257557
1106	88-89	0.16938526775431484
1106	90-91	0.16829298006329907
1106	92-93	0.19384039963017585
1106	94-95	0.20311137868272766
1106	96-97	0.17197665297369724
1106	98-99	0.16843151940485512
1106	100	0.18987485383813407
1107	1	-0.10539200504816648
1107	2	-0.04245851962120639
1107	3	-0.04621986211690654
1107	4	-0.10578177721030357
1107	5	-0.13800386404172116
1107	6	-0.10125487358574503
1107	7	-0.061006426058519025
1107	8	-0.07899050824732257
1107	9	-0.053044510168675174
1107	10-11	-0.034756246064389984
1107	12-13	-0.007179111167751273
1107	14-15	-0.062444740332644244
1107	16-17	-0.03128166051203607
1107	18-19	-0.0065746158677981725
1107	20-21	-0.009221544343873234
1107	22-23	-0.004110433625619692
1107	24-25	0.008404026057803549
1107	26-27	-0.03266366092538675
1107	28-29	-0.04359923885474615
1107	30-31	-0.022382638560650037
1107	32-33	-0.04487613960034764
1107	34-35	-0.05169389807553415
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2216	40-41	-0.8432816225890534
2216	42-43	-0.7907065710530929
2216	44-45	-0.8145472036370336
2216	46-47	-0.7962637603215157
2216	48-49	-0.7932943074331718
2216	50-51	-0.7823248820602089
2216	52-53	-0.8215205667486103
2216	54-55	-0.8822559119658564
2216	56-57	-0.9058876802710003
2216	58-59	-0.97783830908147
2216	60-61	-0.9693362113740491
2216	62-63	-0.975244516922416
2216	64-65	-0.8977655608322692
2216	66-67	-0.8691571406293122
2216	68-69	-0.9250522261252314
2216	70-71	-0.8646747658732252
2216	72-73	-0.9612139845966539
2216	74-75	-0.8833644134433243
2216	76-77	-0.8437145046990864
2216	78-79	-0.7661318292080566
2216	80-81	-0.7790954666643373
2216	82-83	-0.7872291565133551
2216	84-85	-0.826022632466433
2216	86-87	-0.7884535694306436
2216	88-89	-0.6851778846102334
2216	90-91	-0.7087514850055854
2216	92-93	-0.8850466551840697
2216	94-95	-0.8281640809146751
2216	96-97	-0.9611327129295262
2216	98-99	-1.0200525115284975
2216	100	-1.0289280018808569
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	74.0
3	439.0
4	240.0
5	355.0
6	440.0
7	811.0
8	1462.0
9	2488.0
10	3297.0
11	4754.0
12	7426.0
13	10896.0
14	11955.0
15	15479.0
16	20908.0
17	33530.0
18	40691.0
19	20295.0
20	12619.0
21	11644.0
22	11394.0
23	11414.0
24	12690.0
25	15091.0
26	18608.0
27	23703.0
28	29199.0
29	36047.0
30	46011.0
31	57307.0
32	72991.0
33	93965.0
34	131211.0
35	215588.0
36	404589.0
37	922370.0
38	1541925.0
39	798987.0
40	12089.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.68001443737734	21.54108260994267	14.982603175148803	23.79629977753119
2	13.334981746438546	22.44397507874359	40.26331358531569	23.957729589502172
3	15.017512532889704	29.3638043958348	31.898094760160795	23.720588311114703
4	10.406246549303974	18.947688629814273	40.34589239886587	30.30017242201588
5	11.842645148788975	38.88390975518273	35.4069897584996	13.866455337528697
6	27.211190075493306	40.42657952275648	18.897667058648132	13.464563343102078
7	24.327784726127835	34.64597714878382	24.111715147341066	16.914522977747282
8	24.94078387413743	34.50101418222455	21.644552009008844	18.913649934629177
9	26.19067055468743	15.67795536051482	21.970332001283786	36.16104208351397
10-11	23.21769450451151	27.156291904028844	29.430199730095623	20.195813861364016
12-13	24.64585255109472	25.689401162023827	29.40055407303401	20.264192213847444
14-15	22.107625765255374	26.023000733407777	28.233707455796818	23.635666045540027
16-17	20.407748085814294	29.558438679247313	28.13129030359301	21.902522931345384
18-19	20.306254675098636	27.577958411009966	31.845676309811726	20.270110604079672
20-21	21.89730271781932	26.5580296551093	31.51828514052256	20.02638248654882
22-23	21.34648957025258	26.792540502603824	31.52132711246679	20.339642814676804
24-25	20.96841447224263	26.786946453045985	31.44648312130235	20.798155953409037
26-27	20.785774321490237	27.151421735406032	31.36536545675717	20.697438486346563
28-29	20.806750063937336	26.842241111797478	31.679104505667468	20.671904318597726
30-31	21.155628806873096	26.904738938706135	31.257611597921592	20.682020656499176
32-33	20.632991057459616	26.931987131060193	31.630035274541285	20.80498653693891
34-35	21.152570570888628	26.74556411327005	31.21926717839653	20.882598137444788
36-37	21.35677995222352	26.95486090112777	30.805756336925715	20.882602809722993
38-39	21.18450366868502	26.88464198620391	30.926731673143575	21.004122671967494
40-41	21.024995322064324	26.893189033071234	30.92304532645761	21.158770318406834
42-43	21.028355303055854	27.053908223194707	30.91318370248382	21.00455277126562
44-45	21.349352642892843	26.88129482377474	30.455725182066335	21.313627351266085
46-47	21.54752012319252	27.037271839045395	30.087599369944495	21.327608667817586
48-49	21.569903152165377	27.17018775674	29.661294132318574	21.598614958776054
50-51	21.870907902531346	27.32881733516007	28.922309829412136	21.87796493289645
52-53	21.531275523729203	27.534359961005222	29.203023771090848	21.731340744174734
54-55	21.69681838228865	27.671049304623306	29.0737254613843	21.558406851703744
56-57	21.667053888496653	27.485682190878556	29.112228701698374	21.735035218926416
58-59	21.697919054357392	27.103754690436055	29.28096343011989	21.917362825086666
60-61	21.846037312199197	27.509634604166237	29.048990803476414	21.595337280158144
62-63	21.40707525829316	27.22888079910943	29.52539880927574	21.838645133321677
64-65	21.61920282398514	27.092833012028834	29.508094768143035	21.77986939584299
66-67	21.638360258417215	27.21862068209926	29.24973678574198	21.89328227374154
68-69	21.826796299210358	27.262931254400556	29.10099512293627	21.809277323452818
70-71	21.931963886192506	27.285338628976742	28.992852793365607	21.789844691465145
72-73	21.728280436036293	27.263825759060804	29.147679382905984	21.86021442199692
74-75	21.769032574207642	27.151485388122115	29.007843249723493	22.07163878794675
76-77	21.75372166817867	27.33555261055444	28.86721694602048	22.04350877524641
78-79	21.699007064865437	27.394025675753042	28.96183438807382	21.945132871307695
80-81	21.67423030776948	27.406231437750606	28.916560775691224	22.002977478788686
82-83	21.527363790081438	27.365433500704306	29.17856534439086	21.928637364823395
84-85	21.66907110333299	27.089875447280537	28.894389859023857	22.346663590362617
86-87	21.63629235387945	27.370866653419192	28.863377354335952	22.129463638365404
88-89	21.421549672174816	27.262111915994257	28.961271085217362	22.35506732661356
90-91	21.44628696605071	27.152193821882385	28.841258687326704	22.560260524740205
92-93	21.381958008546782	27.248481799206502	29.188425324800875	22.18113486744584
94-95	21.44357239559367	26.925063233924917	29.039677864667585	22.591686505813826
96-97	21.42122889281834	27.35971529191022	28.699386642771994	22.519669172499444
98-99	21.039643496296197	27.11083832657572	29.067131670252333	22.78238650687575
100	21.232851697826387	27.199358774125994	28.77709495118408	22.790694576863537
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1484.0
1	1014.5
2	1508.0
3	2249.5
4	1419.0
5	1074.0
6	2425.5
7	6729.0
8	12497.0
9	13154.5
10	8343.5
11	4797.5
12	4314.0
13	4618.5
14	4904.0
15	5175.0
16	5778.5
17	6787.0
18	7735.0
19	8915.5
20	11109.5
21	13200.5
22	14948.5
23	18302.5
24	24041.5
25	29446.0
26	35714.0
27	45307.5
28	56565.0
29	69888.0
30	81247.0
31	90736.0
32	100731.0
33	107807.5
34	114544.0
35	124175.5
36	132535.5
37	139141.0
38	147622.5
39	154631.5
40	160628.5
41	164684.0
42	162989.5
43	163683.0
44	164760.5
45	161754.0
46	160133.0
47	156195.5
48	146709.0
49	145933.5
50	149406.0
51	143878.5
52	134010.0
53	124638.0
54	117378.5
55	109848.5
56	97469.0
57	83923.5
58	76988.5
59	73978.5
60	66533.5
61	55604.5
62	47337.5
63	40100.0
64	32758.5
65	25214.0
66	18968.5
67	14254.0
68	10663.5
69	8074.0
70	6024.5
71	4487.5
72	3193.5
73	2174.0
74	1635.5
75	1196.0
76	720.0
77	455.0
78	302.0
79	165.5
80	79.0
81	34.5
82	23.5
83	15.5
84	11.5
85	8.5
86	5.5
87	5.5
88	5.0
89	5.5
90	5.5
91	5.5
92	5.5
93	4.5
94	5.5
95	6.0
96	6.0
97	12.0
98	9.0
99	1.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008528496995262279
2	0.0
3	6.44470805687326E-4
4	0.002663812663507614
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	7.518826066352135E-5
24-25	4.296472037915507E-5
26-27	4.1890602369676187E-4
28-29	7.196590663508473E-4
30-31	0.0016971064549766248
32-33	0.004479072099526915
34-35	0.005316884146920439
36-37	0.003899048374408322
38-39	0.0024167655213274725
40-41	0.0032438363886262075
42-43	3.22235402843663E-4
44-45	3.544589431280293E-4
46-47	0.0033082834691949402
48-49	0.0022019419194316972
50-51	0.0010633768293840879
52-53	0.0
54-55	4.940942843602833E-4
56-57	5.907649052133821E-4
58-59	6.659531658769035E-4
60-61	3.544589431280293E-4
62-63	0.0
64-65	0.0
66-67	6.229884454977485E-4
68-69	6.337296255925372E-4
70-71	0.0018582241563984564
72-73	5.370590047394383E-5
74-75	0.0018797065165880342
76-77	2.7927068246450795E-4
78-79	9.774473886257776E-4
80-81	7.948473270143687E-4
82-83	0.0011385650900476091
84-85	9.774473886257776E-4
86-87	1.503765213270427E-4
88-89	0.001331906331753807
90-91	0.0014822828530808496
92-93	0.0027927068246450795
94-95	9.452238483414114E-4
96-97	0.002341577260663951
98-99	0.0023093537203795846
100	0.003179389308057475
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4654982.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.93435203019286
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.4773479210634	30.085079096579946
2	16.313589702084176	14.334539856949908
3	6.149278370375237	8.10493681967149
4	2.943689769028899	5.17316410320771
5	1.6091837002361375	3.5349221583711405
6	1.0148783147953888	2.6752812690017698
7	0.6717180787408692	2.0658048975512306
8	0.4677418231016352	1.643994713231315
9	0.3396150034141089	1.342868860325755
>10	1.768469915602026	14.029633924761994
>50	0.14067464405863223	4.2871492458492835
>100	0.09499465826845106	7.909221703424789
>500	0.006123680517262562	1.8028903161047074
>1k	0.0025474504202451734	1.6251944889828636
>5k	0.0	0.0
>10k+	1.4696829347563364E-4	1.3853185459861463
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29791	0.6399809924077042	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18989	0.40792853763988773	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15584	0.33478110119437626	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	2.148236018957753E-5	0.0
9	4.296472037915506E-5	0.0	0.0	2.148236018957753E-5	0.0
10-11	4.296472037915506E-5	0.0	0.0	2.148236018957753E-5	0.0
12-13	4.296472037915506E-5	0.0	0.0	2.148236018957753E-5	0.0
14-15	8.592944075831012E-5	0.0	0.0	2.148236018957753E-5	0.0
16-17	8.592944075831012E-5	0.0	0.0	2.148236018957753E-5	0.0
18-19	1.0741180094788765E-4	0.0	0.0	2.148236018957753E-5	0.0
20-21	1.288941611374652E-4	0.0	0.0	7.518826066352137E-5	0.0
22-23	1.288941611374652E-4	0.0	0.0	3.0075304265408546E-4	0.0
24-25	1.288941611374652E-4	0.0	0.0	6.44470805687326E-4	0.0
26-27	1.288941611374652E-4	0.0	0.0	0.0010204121090049327	0.0
28-29	1.288941611374652E-4	0.0	0.0	0.0026315891232232477	0.0
30-31	1.288941611374652E-4	0.0	0.0	0.006960284701423121	0.0
32-33	1.288941611374652E-4	0.0	0.0	0.014275028345974269	0.0
34-35	1.5037652132704273E-4	0.0	0.0	0.025263255582943178	0.0
36-37	1.5037652132704273E-4	0.0	0.0	0.040923896161145196	0.0
38-39	1.5037652132704273E-4	1.0741180094788765E-5	0.0	0.06499488075356682	0.0
40-41	1.5037652132704273E-4	2.148236018957753E-5	0.0	0.09320121968248213	0.0
42-43	1.5037652132704273E-4	2.148236018957753E-5	0.0	0.11959229917537813	0.0
44-45	1.611177014218315E-4	2.148236018957753E-5	0.0	0.1500220623839147	0.0
46-47	1.7185888151662024E-4	2.148236018957753E-5	0.0	0.18669245122752354	0.0
48-49	1.82600061611409E-4	2.148236018957753E-5	0.0	0.2266066764597586	0.0
50-51	1.9334124170619779E-4	2.148236018957753E-5	0.0	0.2723855860237483	0.0
52-53	2.1482360189577533E-4	2.148236018957753E-5	0.0	0.31657480093370927	0.0
54-55	2.1482360189577533E-4	2.148236018957753E-5	0.0	0.3639111816114434	0.0
56-57	2.577883222749304E-4	2.148236018957753E-5	0.0	0.4189812119574254	0.0
58-59	3.0075304265408546E-4	2.148236018957753E-5	0.0	0.48329939836502056	0.0
60-61	3.437177630332405E-4	2.148236018957753E-5	0.0	0.555888293445603	0.0
62-63	4.7261192417070573E-4	2.148236018957753E-5	0.0	0.6321828956588876	0.0
64-65	4.8335310426549446E-4	2.148236018957753E-5	0.0	0.7137191937584291	0.0
66-67	4.940942843602832E-4	2.148236018957753E-5	0.0	0.8023446707205313	0.0
68-69	4.940942843602832E-4	2.148236018957753E-5	0.0	0.9032902812513561	0.0
70-71	4.940942843602832E-4	2.148236018957753E-5	0.0	1.0251489694267346	0.0
72-73	4.940942843602832E-4	2.148236018957753E-5	0.0	1.1565887902466647	0.0
74-75	5.370590047394383E-4	2.148236018957753E-5	0.0	1.2875559991424241	0.0
76-77	5.370590047394383E-4	2.148236018957753E-5	0.0	1.4283621290050101	0.0
78-79	5.370590047394383E-4	2.148236018957753E-5	0.0	1.5897913246495905	0.0
80-81	5.370590047394383E-4	2.148236018957753E-5	0.0	1.775968199232564	0.0
82-83	5.370590047394383E-4	2.148236018957753E-5	0.0	1.9787939029624604	0.0
84-85	5.370590047394383E-4	2.148236018957753E-5	0.0	2.1889343503369076	0.0
86-87	5.370590047394383E-4	2.148236018957753E-5	0.0	2.4071521651426364	0.0
88	5.370590047394383E-4	4.296472037915506E-5	0.0	2.581277435659257	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9255	0.0	64.19637	1
GTATCAA	21505	0.0	50.90611	1
ATCAACG	28765	0.0	37.825077	3
TCAACGC	29010	0.0	37.538033	4
TATCAAC	29600	0.0	36.837444	2
CAACGCA	30375	0.0	35.834896	5
AACGCAG	31225	0.0	34.919617	6
ACGCAGA	34255	0.0	31.679907	7
CGCAGAG	34670	0.0	31.12447	8
GCAGAGT	36965	0.0	28.912367	9
GTGGTAT	2950	0.0	27.087366	1
GTACATG	22685	0.0	24.926813	1
TACATGG	22150	0.0	24.741045	2
AGAGTAC	35350	0.0	24.004463	10-11
TGGTATC	3345	0.0	23.464624	2
ACATGGG	22875	0.0	23.258331	3
AGTACTT	25125	0.0	23.195274	12-13
GAGTACT	24660	0.0	22.279495	12-13
CAGAGTA	36495	0.0	22.118074	10-11
CATGGGG	14815	0.0	20.620773	4
>>END_MODULE