FastQCFastQC Report
Wed 25 May 2016
SRR2431371_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431371_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4585865
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130430.2844174436011527No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47600.043.6429751
GTATCAA85750.039.573281
TCAACGC98100.033.2977454
ATCAACG98400.033.1962283
CAACGCA102700.031.6686785
AACGCAG108800.030.022736
TATCAAC123650.027.0255472
ACGCAGA122050.026.4553327
CGCAGAG122500.025.8977458
GTACATG188050.024.9434321
TACATGG180250.024.3279442
ACATGGG192350.022.2600023
GCAGAGT143950.021.3857239
CATGGGG106900.019.6090344
AGAGTAC124200.017.7289210-11
CTGTCGC9650.017.5335359
CAGAGTA137400.017.03479610-11
GTATTAG25600.016.5234811
GTATATA42150.015.7224621
GAGTACA137400.015.6667091
Produced by FastQC (version 0.11.5)