##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431371_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4585865
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.94080113566361	33.0	31.0	34.0	27.0	34.0
2	30.99334803793832	33.0	31.0	34.0	27.0	34.0
3	31.089766270921626	34.0	31.0	34.0	27.0	34.0
4	34.35952780991154	37.0	35.0	37.0	31.0	37.0
5	34.484322804967	37.0	35.0	37.0	31.0	37.0
6	34.516780803621565	37.0	35.0	37.0	31.0	37.0
7	34.40607606198613	37.0	35.0	37.0	31.0	37.0
8	34.363140650673316	37.0	35.0	37.0	31.0	37.0
9	35.81568886131624	39.0	37.0	39.0	30.0	39.0
10-11	35.84012645378789	39.0	37.0	39.0	30.0	39.0
12-13	35.80998099595169	39.0	37.0	39.0	30.0	39.0
14-15	37.142051172461464	40.0	38.0	41.0	31.0	41.0
16-17	37.14504984337742	40.0	38.0	41.0	31.0	41.0
18-19	37.164825610871674	40.0	38.0	41.0	31.0	41.0
20-21	37.205712335622614	40.0	38.0	41.0	30.5	41.0
22-23	37.210968922983994	40.0	38.0	41.0	30.0	41.0
24-25	37.23964879035907	40.0	38.0	41.0	30.0	41.0
26-27	37.23474415404728	40.0	38.0	41.0	30.0	41.0
28-29	37.20507734091605	40.0	38.0	41.0	30.0	41.0
30-31	37.136031261277864	40.0	38.0	41.0	30.0	41.0
32-33	37.0609473894238	40.0	38.0	41.0	30.0	41.0
34-35	36.99646773291407	40.0	38.0	41.0	30.0	41.0
36-37	36.9042945006013	40.0	38.0	41.0	30.0	41.0
38-39	36.80130651469243	40.0	37.5	41.0	30.0	41.0
40-41	36.71196132027437	40.0	37.0	41.0	30.0	41.0
42-43	36.59006196213801	40.0	37.0	41.0	29.0	41.0
44-45	36.45541735310569	40.0	37.0	41.0	29.0	41.0
46-47	36.32677226651897	40.0	37.0	41.0	29.0	41.0
48-49	36.18945215351957	40.0	36.0	41.0	28.0	41.0
50-51	36.0524069068758	40.0	36.0	41.0	28.0	41.0
52-53	35.888931750062426	40.0	35.5	41.0	28.0	41.0
54-55	35.728072348401014	39.5	35.0	41.0	28.0	41.0
56-57	35.532346024141575	39.0	35.0	41.0	27.5	41.0
58-59	35.31754085652325	39.0	35.0	41.0	27.0	41.0
60-61	35.15115501655631	39.0	35.0	41.0	27.0	41.0
62-63	35.00096917375457	39.0	35.0	40.5	26.5	41.0
64-65	34.764745364287876	38.0	34.0	40.0	26.0	41.0
66-67	34.469347876572904	38.0	34.0	40.0	26.0	41.0
68-69	34.164408350442066	37.0	34.0	40.0	26.0	41.0
70-71	33.917262828277764	37.0	34.0	40.0	26.0	41.0
72-73	33.67272052273671	36.0	34.0	39.0	25.5	41.0
74-75	33.328838943143765	36.0	33.5	39.0	25.0	41.0
76-77	32.96879716258547	35.5	33.0	38.5	24.5	40.0
78-79	32.6165778975177	35.0	33.0	37.5	24.0	39.5
80-81	32.25479566014263	35.0	33.0	37.0	23.0	39.0
82-83	31.89935366610225	35.0	33.0	36.5	22.0	39.0
84-85	31.591313852457496	35.0	32.0	36.0	20.5	37.5
86-87	31.303766901118983	35.0	32.0	36.0	20.0	37.0
88-89	31.03786068713318	35.0	32.0	35.5	19.5	37.0
90-91	30.810711174445824	35.0	32.0	35.0	18.0	36.0
92-93	30.590965499420502	35.0	32.0	35.0	16.0	36.0
94-95	30.36390201194322	35.0	31.5	35.0	8.5	36.0
96-97	30.16106318001075	35.0	31.0	35.0	2.0	36.0
98-99	30.024889851750977	35.0	31.5	35.0	2.0	35.0
100	29.973524515004257	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.6789789171951846
1101	2	0.6307503642832764
1101	3	0.6451834978896223
1101	4	0.6703587591544036
1101	5	0.5936485524175623
1101	6	0.6554767334446012
1101	7	0.643641481519559
1101	8	0.6641469682613916
1101	9	0.7790967287433759
1101	10-11	0.7544777230762207
1101	12-13	0.7262379549663223
1101	14-15	0.9194344076318117
1101	16-17	0.917912737298856
1101	18-19	0.8396227918742767
1101	20-21	0.8668394936390698
1101	22-23	0.8744563481694954
1101	24-25	0.8559762896887051
1101	26-27	0.9035821656727094
1101	28-29	0.9033507074210618
1101	30-31	0.8929247725282039
1101	32-33	0.8708814076662463
1101	34-35	0.8598694937810762
1101	36-37	0.9117720157824749
1101	38-39	0.8854056451325931
1101	40-41	0.8955223427002679
1101	42-43	0.8644098215009564
1101	44-45	0.843398726181519
1101	46-47	0.875958245024762
1101	48-49	0.8889127590148149
1101	50-51	0.8402491581596223
1101	52-53	0.8617531203391522
1101	54-55	0.9244898296321722
1101	56-57	0.8188065173845089
1101	58-59	0.8430417792320171
1101	60-61	0.8926365548632162
1101	62-63	0.8626461710892741
1101	64-65	0.8516280153602125
1101	66-67	0.7987873377766022
1101	68-69	0.8641820150252926
1101	70-71	0.7513359938439308
1101	72-73	0.84441317630165
1101	74-75	0.9005993180214062
1101	76-77	0.9048747998909619
1101	78-79	0.8630594154265339
1101	80-81	0.8439151846176358
1101	82-83	0.9412823923558449
1101	84-85	0.8918280002967123
1101	86-87	0.8926146254702125
1101	88-89	0.8850341335117804
1101	90-91	0.8352760095916913
1101	92-93	0.8620575510452113
1101	94-95	0.9453312374430602
1101	96-97	0.9399099686236667
1101	98-99	0.9894432916205034
1101	100	0.9849010493340451
1102	1	0.4283525755588542
1102	2	0.49388036947765457
1102	3	0.4810863489608046
1102	4	0.4320798760364539
1102	5	0.4058724064468109
1102	6	0.43647381558855614
1102	7	0.4751033973321981
1102	8	0.5232362491042792
1102	9	0.5805579523281708
1102	10-11	0.5827311005341897
1102	12-13	0.591484326686988
1102	14-15	0.6988402554801283
1102	16-17	0.7825244481924756
1102	18-19	0.7057640771794524
1102	20-21	0.673962126695848
1102	22-23	0.5762903248832316
1102	24-25	0.632459921703223
1102	26-27	0.6683939286346714
1102	28-29	0.6903819165758378
1102	30-31	0.6793707021483897
1102	32-33	0.7111668261980597
1102	34-35	0.6917123128602967
1102	36-37	0.7210908262111602
1102	38-39	0.6871815743595064
1102	40-41	0.6534366512990104
1102	42-43	0.6218652844688108
1102	44-45	0.6438916315578851
1102	46-47	0.6331335247675653
1102	48-49	0.5468396487212104
1102	50-51	0.5905163065029768
1102	52-53	0.6402775788488171
1102	54-55	0.6107872060886379
1102	56-57	0.5529684243174131
1102	58-59	0.5962303426314506
1102	60-61	0.6243752194542651
1102	62-63	0.6526218399032686
1102	64-65	0.65232974576967
1102	66-67	0.6945904083854444
1102	68-69	0.659032996520736
1102	70-71	0.6272908100503187
1102	72-73	0.6472941787756952
1102	74-75	0.6334599611669205
1102	76-77	0.606598269279985
1102	78-79	0.6284374172085307
1102	80-81	0.6107403696818139
1102	82-83	0.5601850706369902
1102	84-85	0.5791913008675564
1102	86-87	0.6003138540721373
1102	88-89	0.6384058080821227
1102	90-91	0.6705756970926373
1102	92-93	0.66532070244007
1102	94-95	0.6819572263812965
1102	96-97	0.7224908111379165
1102	98-99	0.6851327229740107
1102	100	0.6888417832414824
1103	1	0.3158767620576306
1103	2	0.2752808993005367
1103	3	0.3823123010213756
1103	4	0.3180243105810163
1103	5	0.32372197238773737
1103	6	0.2929408889766023
1103	7	0.38446629092844375
1103	8	0.42924793579598486
1103	9	0.4748869813182921
1103	10-11	0.44605203835998
1103	12-13	0.4025245425729693
1103	14-15	0.43198600167308143
1103	16-17	0.3934639191553444
1103	18-19	0.35853540444470866
1103	20-21	0.4377195790840531
1103	22-23	0.40595239091184965
1103	24-25	0.3812476151075188
1103	26-27	0.5278502007077037
1103	28-29	0.5028525384863229
1103	30-31	0.4735266722672691
1103	32-33	0.44366651750888053
1103	34-35	0.48090496437406927
1103	36-37	0.4956983524742995
1103	38-39	0.439686779981578
1103	40-41	0.4594390948544884
1103	42-43	0.40585310317284495
1103	44-45	0.40842264850632404
1103	46-47	0.38398698019214805
1103	48-49	0.3780970765545888
1103	50-51	0.3159627131504621
1103	52-53	0.27351037188254423
1103	54-55	0.263375863024109
1103	56-57	0.16369698690624546
1103	58-59	0.1683686360854182
1103	60-61	0.18156646713383395
1103	62-63	0.25160627004199654
1103	64-65	0.2601510175410269
1103	66-67	0.26745499836903974
1103	68-69	0.2814090507995246
1103	70-71	0.2788156293619082
1103	72-73	0.2560047735495914
1103	74-75	0.31061001638018837
1103	76-77	0.320045611816937
1103	78-79	0.2815029638479558
1103	80-81	0.25566081907713567
1103	82-83	0.28206857274379615
1103	84-85	0.24702876478951197
1103	86-87	0.2265110769920291
1103	88-89	0.28167915515680164
1103	90-91	0.3204143894572091
1103	92-93	0.3795093374866738
1103	94-95	0.3083322086667444
1103	96-97	0.2828079752384838
1103	98-99	0.34046687199511894
1103	100	0.3519017559365203
1104	1	0.298790384662901
1104	2	0.3465316894396011
1104	3	0.3433536967108637
1104	4	0.31355793555670886
1104	5	0.3145481919369928
1104	6	0.3752369115381171
1104	7	0.3451561127216962
1104	8	0.4258264057220771
1104	9	0.3621128638428459
1104	10-11	0.37022765360480037
1104	12-13	0.3919157860087381
1104	14-15	0.41371156662250996
1104	16-17	0.44822682425356675
1104	18-19	0.443828542238883
1104	20-21	0.45875313796156547
1104	22-23	0.42553084032471133
1104	24-25	0.4845601021917858
1104	26-27	0.48294086156786165
1104	28-29	0.5421742078982135
1104	30-31	0.5533492366636068
1104	32-33	0.5066364223275102
1104	34-35	0.5140950786707279
1104	36-37	0.4872132845831132
1104	38-39	0.5012496294661659
1104	40-41	0.49192701104504266
1104	42-43	0.47786449713010626
1104	44-45	0.4295797615813086
1104	46-47	0.4474423646859691
1104	48-49	0.38266449820925885
1104	50-51	0.3052532898304179
1104	52-53	0.35142178741667607
1104	54-55	0.303633448359129
1104	56-57	0.3212972509950305
1104	58-59	0.3385924163842091
1104	60-61	0.35223099687952697
1104	62-63	0.2804180452413476
1104	64-65	0.35934139091212813
1104	66-67	0.4076091438640361
1104	68-69	0.3997652348533052
1104	70-71	0.391376352308761
1104	72-73	0.3261897306693484
1104	74-75	0.39078909304191
1104	76-77	0.39466617412244176
1104	78-79	0.41053143848449736
1104	80-81	0.44215101755398933
1104	82-83	0.4327061162014445
1104	84-85	0.4261202416202039
1104	86-87	0.4380652083556882
1104	88-89	0.47834381861834174
1104	90-91	0.4596922593334618
1104	92-93	0.39625164350753295
1104	94-95	0.3450868758054675
1104	96-97	0.341966028882851
1104	98-99	0.3361001190716024
1104	100	0.3446581180754862
1105	1	0.28925973165707575
1105	2	0.3112980757960919
1105	3	0.3256072420249865
1105	4	0.33958201671466526
1105	5	0.2694400396415091
1105	6	0.2232195246496147
1105	7	0.2856650767025428
1105	8	0.361534384133563
1105	9	0.27666486127954926
1105	10-11	0.33552087314902224
1105	12-13	0.4001918066420629
1105	14-15	0.5028854457092748
1105	16-17	0.4662819778246501
1105	18-19	0.38176842398114275
1105	20-21	0.41441618972775984
1105	22-23	0.42729993148043377
1105	24-25	0.4729942611025635
1105	26-27	0.46007489847267635
1105	28-29	0.45989341527887717
1105	30-31	0.4710769927741012
1105	32-33	0.4741396302265741
1105	34-35	0.471675265756609
1105	36-37	0.4780510771102584
1105	38-39	0.4450400777105443
1105	40-41	0.4142230950277721
1105	42-43	0.41838869296384473
1105	44-45	0.40304100910264395
1105	46-47	0.35860198052399994
1105	48-49	0.39161993032724496
1105	50-51	0.40025244345893896
1105	52-53	0.40568409437596387
1105	54-55	0.36242981742545766
1105	56-57	0.3782327835591772
1105	58-59	0.3557849275457343
1105	60-61	0.4958654803964464
1105	62-63	0.509409470818099
1105	64-65	0.4775053825662994
1105	66-67	0.4105025346851363
1105	68-69	0.3781110187897738
1105	70-71	0.4818753081357343
1105	72-73	0.4019844933301613
1105	74-75	0.4238096955752866
1105	76-77	0.42759056802221806
1105	78-79	0.41784874976632835
1105	80-81	0.35375778863921425
1105	82-83	0.40353572461860665
1105	84-85	0.44292950047083224
1105	86-87	0.4025000598552744
1105	88-89	0.44558201589241975
1105	90-91	0.48821958334109183
1105	92-93	0.41597722361206024
1105	94-95	0.47140685164609764
1105	96-97	0.44405774721344216
1105	98-99	0.4814194344401983
1105	100	0.46191698343894316
1106	1	0.14943570687202978
1106	2	0.1613384443142536
1106	3	0.1809217443056781
1106	4	0.16402589878254759
1106	5	0.14206913352151673
1106	6	0.1603611883754823
1106	7	0.1077234070220996
1106	8	0.10911783356652194
1106	9	0.14717955550772643
1106	10-11	0.13335016773325492
1106	12-13	0.16595474974347013
1106	14-15	0.2091122020681908
1106	16-17	0.1565548287264633
1106	18-19	0.17727134040954695
1106	20-21	0.2160857475742759
1106	22-23	0.253927654056767
1106	24-25	0.2574221271141113
1106	26-27	0.28985300735208597
1106	28-29	0.2987862495604148
1106	30-31	0.31691393460928907
1106	32-33	0.28441775267891956
1106	34-35	0.31717450435906613
1106	36-37	0.30812783421350076
1106	38-39	0.23743437743058848
1106	40-41	0.25601499750583656
1106	42-43	0.2246070428634468
1106	44-45	0.276414753278317
1106	46-47	0.28669687106051356
1106	48-49	0.32406463815462416
1106	50-51	0.32005937511004845
1106	52-53	0.25119600834285905
1106	54-55	0.28119264055968785
1106	56-57	0.30741311437085983
1106	58-59	0.31899031486020846
1106	60-61	0.32431968139313483
1106	62-63	0.32242249470031936
1106	64-65	0.34998723205739424
1106	66-67	0.3740144731708668
1106	68-69	0.3378061839372961
1106	70-71	0.28670337415095304
1106	72-73	0.23268827597414798
1106	74-75	0.3006528555633423
1106	76-77	0.2760806015517332
1106	78-79	0.27897949297614133
1106	80-81	0.22504541739596107
1106	82-83	0.26338704412623315
1106	84-85	0.26669552418930564
1106	86-87	0.30183840145350516
1106	88-89	0.308768291647052
1106	90-91	0.2612275151857233
1106	92-93	0.2683894177066968
1106	94-95	0.3042305725726635
1106	96-97	0.232357926113405
1106	98-99	0.2938326443153336
1106	100	0.3332600203949667
1107	1	0.02700347530722169
1107	2	0.08931292235376276
1107	3	0.14365531105981333
1107	4	0.14660439655367696
1107	5	0.0449311020234191
1107	6	0.11207818672001224
1107	7	0.1137846061186707
1107	8	0.14306578753012644
1107	9	0.22006251056026116
1107	10-11	0.13755059995263252
1107	12-13	0.1884137475366856
1107	14-15	0.18711382707904534
1107	16-17	0.1752526954999425
1107	18-19	0.2019960251924715
1107	20-21	0.10333527583377133
1107	22-23	0.15427314019696325
1107	24-25	0.1845829955735141
1107	26-27	0.17559955287143225
1107	28-29	0.14180555627321922
1107	30-31	0.18459571270926034
1107	32-33	0.18144312234223037
1107	34-35	0.24382704292451507
1107	36-37	0.20983938793404633
1107	38-39	0.21628033797648527
1107	40-41	0.2239631814381795
1107	42-43	0.1538245323663645
1107	44-45	0.15715574756565331
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2216	98-99	-1.822716993203997
2216	100	-1.8378735286424934
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	96306.0
3	19549.0
4	13714.0
5	11719.0
6	8871.0
7	8675.0
8	9653.0
9	10208.0
10	10472.0
11	11352.0
12	12792.0
13	13237.0
14	13500.0
15	13125.0
16	13260.0
17	13379.0
18	13304.0
19	13007.0
20	12437.0
21	13235.0
22	15895.0
23	24497.0
24	39361.0
25	49285.0
26	44545.0
27	36032.0
28	38657.0
29	46344.0
30	55647.0
31	67021.0
32	87352.0
33	116641.0
34	166175.0
35	257906.0
36	455731.0
37	863126.0
38	1307582.0
39	579887.0
40	2386.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.160376348412086	22.292910572658503	14.195710856226002	22.35100222270341
2	14.460630655285318	23.00405703177045	40.49255702032223	22.042755292622
3	16.35894209707438	29.84830560864744	31.304672073861745	22.488080220416432
4	11.365164775928811	19.80529732238025	40.96608621625064	27.8634516854403
5	11.527247313211356	40.842523711448116	35.749155284771795	11.881073690568737
6	26.53604063791673	41.97260495021114	19.317424302721513	12.173930109150618
7	23.653574625506856	35.63107505345229	24.7997705994398	15.91557972160105
8	22.985478203130707	37.8825150762179	22.057845139357568	17.074161581293822
9	24.55460420226064	18.49354483832385	22.05945879348825	34.892392165927255
10-11	21.755110540759485	29.496659844980172	30.134020517394212	18.61420909686613
12-13	22.646022069991155	27.94013779297908	29.417787047808865	19.9960530892209
14-15	20.687253113643774	28.907676087281242	27.048168666107703	23.35690213296728
16-17	20.140584164601442	31.918896435023708	27.3412649521955	20.59925444817935
18-19	20.138610709211893	30.89098785071083	29.18254244291971	19.78785899715757
20-21	21.86778285012751	29.849886553572773	28.406625576636035	19.875705019663684
22-23	21.22549399077382	29.977659612744816	28.469252801816015	20.32759359466535
24-25	20.957278506890194	30.00464470715994	28.339909700787093	20.698167085162776
26-27	20.83363771066091	30.043361503227857	28.333051670731695	20.789949115379542
28-29	20.8412916647132	29.80076823020303	28.69188255650788	20.666057548575896
30-31	21.196339185737042	30.044593549962766	28.21964885577748	20.53941840852271
32-33	20.57840778130189	29.91877214004337	28.796900911823613	20.705919166831123
34-35	21.06463011885435	29.56493485961754	28.596219033922715	20.77421598760539
36-37	21.331046596443638	29.85175097827782	28.07790896592028	20.73929345935827
38-39	20.990958085333954	29.80248001194976	28.402787696541438	20.803774206174843
40-41	20.939015867235515	29.64630445946403	28.557000696706076	20.857678976594382
42-43	20.752693330484	29.649880665915806	28.650527217874927	20.946898785725267
44-45	20.88772783324411	29.534537104777396	28.543208315116125	21.03452674686237
46-47	21.073581538052256	29.741869854433133	28.4098964971712	20.774652110343414
48-49	20.970918245521837	29.47572595355511	28.576844281286085	20.976511519636972
50-51	21.079883511616675	29.55324677023855	28.174063126585715	21.192806591559062
52-53	20.845282187766102	29.61702972067429	28.436968816133923	21.100719275425682
54-55	21.049583884392582	29.76941100533923	28.21431725530516	20.966687854963023
56-57	20.989540686435383	29.65580103208446	28.22679036561259	21.12786791586756
58-59	20.96977342333453	29.217116072976417	28.43728500511899	21.375825498570062
60-61	21.338776871975078	29.53192036834926	28.133710870250216	20.99559188942544
62-63	20.873837324038107	29.601645491090554	28.36944611321964	21.155071071651697
64-65	21.00599341672727	29.34351534552369	28.39495929339394	21.2555319443551
66-67	20.927731191389192	29.357067859607728	28.281087646496356	21.434113302506724
68-69	21.172406950488075	29.51939274269958	28.214415382921214	21.093784923891132
70-71	21.242840772678655	29.421723055519518	28.21829687528961	21.117139296512217
72-73	20.943965860312066	29.459578509179842	28.42856255035855	21.167893080149547
74-75	20.96561935425487	29.310119246859646	28.438080929115884	21.286180469769608
76-77	21.013396600205194	29.58850729360764	28.209618032802975	21.188478073384193
78-79	21.005350135688687	29.601176659147182	28.309250272304137	21.084222932859994
80-81	21.10323788423776	29.53834227566664	28.255519950979803	21.102899889115793
82-83	20.780521077891724	29.595531655516265	28.567403776762024	21.056543489829988
84-85	20.94289262478916	29.293803636633243	28.274261939210632	21.489041799366966
86-87	21.1225434814228	29.583438821808762	28.161148883435832	21.13286881333261
88-89	20.84652192884995	29.3645355786238	28.41277601735999	21.37616647516626
90-91	20.8903415060494	29.39171729092715	28.250256337791058	21.467684865232396
92-93	20.87716128646775	29.506050386395426	28.41659594989425	21.200192377242573
94-95	20.89681808730664	29.12272602727628	28.468409787256455	21.512046098160628
96-97	20.77793107779507	29.496318946028584	28.297809379824017	21.427940596352332
98-99	20.553099038523815	29.373925069361444	28.44634729110392	21.626628601010825
100	20.704437154199155	29.405817871606978	28.211286660450284	21.678458313743583
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	10293.0
1	7454.0
2	3763.5
3	2381.0
4	1660.5
5	1374.5
6	1269.5
7	1307.5
8	1439.5
9	1515.0
10	1330.0
11	1152.5
12	1186.0
13	1290.0
14	1387.0
15	1512.5
16	1807.5
17	2266.0
18	2843.0
19	3715.5
20	5129.0
21	7142.0
22	10236.0
23	14711.5
24	20715.5
25	28631.5
26	39044.5
27	52382.5
28	68041.0
29	84388.5
30	99510.5
31	113647.5
32	127032.0
33	137578.5
34	146490.0
35	155398.0
36	162918.0
37	169860.5
38	176572.5
39	180336.5
40	182793.5
41	182756.5
42	178315.5
43	174560.0
44	169373.0
45	160413.0
46	152779.0
47	144954.0
48	134392.0
49	128416.5
50	125497.5
51	118638.5
52	109316.0
53	100766.0
54	93244.5
55	85880.5
56	76806.0
57	67068.5
58	59753.5
59	53362.0
60	46526.5
61	39415.5
62	32637.5
63	26032.5
64	20716.0
65	16826.0
66	13326.5
67	10569.0
68	8461.0
69	6446.5
70	4994.5
71	4385.0
72	3359.5
73	2069.5
74	1333.0
75	918.5
76	649.5
77	478.0
78	349.5
79	246.0
80	194.5
81	166.5
82	132.5
83	118.0
84	106.5
85	88.5
86	85.5
87	73.0
88	63.5
89	60.5
90	42.0
91	38.5
92	35.0
93	28.0
94	29.5
95	25.0
96	18.5
97	15.5
98	19.5
99	26.5
100	59.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.7444909520886463E-4
2	0.0
3	0.0
4	2.180613690110808E-5
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	1.1993375295609444E-4
84-85	6.541841070332424E-4
86-87	0.0012211436664620525
88-89	0.0032273082613639955
90-91	0.002594930291231861
92-93	0.0018208124312425245
94-95	0.0018644247050447408
96-97	0.0018753277734952947
98-99	0.00226783823771524
100	0.0029002162078473745
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4585865.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.621397658132054
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.70502615168343	49.43702368831801
2	14.04433534814272	17.870404880566966
3	4.174778002723755	7.9681563433713105
4	1.6420689632534207	4.1788288997289005
5	0.7593771726994166	2.41563185384088
6	0.4297413712319171	1.6404448001578045
7	0.2592098850029622	1.1543906619484496
8	0.17795639496217378	0.9057467655756851
9	0.13021385420745044	0.7455948659217212
>10	0.6058788721557267	6.923890550009866
>50	0.041354069071946976	1.8300113618609086
>100	0.027248442427302788	3.291668517254418
>500	0.002400037848922779	1.022415385816976
>1k	3.7714837325566954E-4	0.3312796450951567
>5k	0.0	0.0
>10k+	3.4286215750297785E-5	0.2845117805330569
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	13043	0.2844174436011527	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.180613690110808E-5	0.0
10-11	0.0	0.0	0.0	4.361227380221616E-5	0.0
12-13	1.090306845055404E-5	0.0	0.0	6.541841070332423E-5	0.0
14-15	4.361227380221616E-5	0.0	0.0	6.541841070332423E-5	0.0
16-17	4.361227380221616E-5	0.0	0.0	7.632147915387827E-5	0.0
18-19	4.361227380221616E-5	0.0	0.0	8.722454760443232E-5	0.0
20-21	4.361227380221616E-5	0.0	0.0	1.5264295830775653E-4	0.0
22-23	4.361227380221616E-5	0.0	0.0	4.797350118243777E-4	0.0
24-25	4.361227380221616E-5	0.0	0.0	9.485669551982014E-4	0.0
26-27	4.361227380221616E-5	0.0	0.0	0.0016136541306819977	0.0
28-29	4.361227380221616E-5	0.0	0.0	0.0036961402047378192	0.0
30-31	4.361227380221616E-5	0.0	0.0	0.00828633202242107	0.0
32-33	4.361227380221616E-5	0.0	0.0	0.017335878836380922	0.0
34-35	4.361227380221616E-5	0.0	0.0	0.02952550936410034	0.0
36-37	4.361227380221616E-5	0.0	0.0	0.04740654162300896	0.0
38-39	4.361227380221616E-5	0.0	0.0	0.07381377341025085	0.0
40-41	4.361227380221616E-5	0.0	0.0	0.10417881904504384	0.0
42-43	4.361227380221616E-5	0.0	0.0	0.1331809811235176	0.0
44-45	4.361227380221616E-5	0.0	0.0	0.16791815720698278	0.0
46-47	4.361227380221616E-5	0.0	0.0	0.20867382707515375	0.0
48-49	4.361227380221616E-5	0.0	0.0	0.2543903930883269	0.0
50-51	4.361227380221616E-5	0.0	0.0	0.3044790895501721	0.0
52-53	4.361227380221616E-5	0.0	0.0	0.3527796827861265	0.0
54-55	4.361227380221616E-5	0.0	0.0	0.40274844549501565	0.0
56-57	4.361227380221616E-5	0.0	0.0	0.46492864486852536	0.0
58-59	4.361227380221616E-5	0.0	0.0	0.5355805284281154	0.0
60-61	4.361227380221616E-5	0.0	0.0	0.6136792077394342	0.0
62-63	6.541841070332424E-5	0.0	0.0	0.6972839366182825	0.0
64-65	8.722454760443232E-5	0.0	0.0	0.7843122289906048	0.0
66-67	8.722454760443232E-5	0.0	0.0	0.8839008562179654	0.0
68-69	8.722454760443232E-5	0.0	0.0	0.997423604925134	0.0
70-71	8.722454760443232E-5	0.0	0.0	1.128053268031222	0.0
72-73	8.722454760443232E-5	0.0	0.0	1.2681140853470392	0.0
74-75	8.722454760443232E-5	0.0	0.0	1.4114458231980227	0.0
76-77	8.722454760443232E-5	0.0	0.0	1.5649392208449224	0.0
78-79	8.722454760443232E-5	0.0	0.0	1.7402060461875786	0.0
80-81	8.722454760443232E-5	0.0	0.0	1.9388490502882225	0.0
82-83	8.722454760443232E-5	0.0	0.0	2.156496102698182	0.0
84-85	8.722454760443232E-5	0.0	0.0	2.3785370916937154	0.0
86-87	8.722454760443232E-5	0.0	0.0	2.6170526171180355	0.0
88	8.722454760443232E-5	0.0	0.0	2.8063625946250053	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4760	0.0	43.642975	1
GTATCAA	8575	0.0	39.57328	1
TCAACGC	9810	0.0	33.297745	4
ATCAACG	9840	0.0	33.196228	3
CAACGCA	10270	0.0	31.668678	5
AACGCAG	10880	0.0	30.02273	6
TATCAAC	12365	0.0	27.025547	2
ACGCAGA	12205	0.0	26.455332	7
CGCAGAG	12250	0.0	25.897745	8
GTACATG	18805	0.0	24.943432	1
TACATGG	18025	0.0	24.327944	2
ACATGGG	19235	0.0	22.260002	3
GCAGAGT	14395	0.0	21.385723	9
CATGGGG	10690	0.0	19.609034	4
AGAGTAC	12420	0.0	17.72892	10-11
CTGTCGC	965	0.0	17.533535	9
CAGAGTA	13740	0.0	17.034796	10-11
GTATTAG	2560	0.0	16.523481	1
GTATATA	4215	0.0	15.722462	1
GAGTACA	13740	0.0	15.666709	1
>>END_MODULE