FastQCFastQC Report
Wed 25 May 2016
SRR2431371_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431371_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4585865
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT235430.5133818810627875No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161000.35107880410784004No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119830.2613029384859781No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83100.062.9564551
GTATCAA183750.050.087741
ATCAACG243900.037.5380023
TCAACGC244100.037.5072484
CAACGCA253100.036.190915
TATCAAC259800.035.3130072
AACGCAG260900.035.1809846
ACGCAGA289900.031.5806127
CGCAGAG291050.031.2943538
GCAGAGT315400.028.5951989
GTGGTAT29900.025.3105051
GTACATG179700.024.195791
TACATGG172600.024.153322
AGAGTAC300400.023.83539610-11
TGGTATC31400.023.7991522
GAGTACT214400.022.83044212-13
ACATGGG178650.022.5198143
AGTACTT227700.022.40508512-13
CAGAGTA311950.022.16192810-11
GTACTTT245600.019.67182214-15
Produced by FastQC (version 0.11.5)