##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2431371_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4585865 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.78798045734011 34.0 31.0 34.0 31.0 34.0 2 32.90718653950781 34.0 31.0 34.0 31.0 34.0 3 32.98286866272775 34.0 31.0 34.0 31.0 34.0 4 36.37204169769498 37.0 37.0 37.0 35.0 37.0 5 36.33168246339567 37.0 37.0 37.0 35.0 37.0 6 36.36402685207698 37.0 37.0 37.0 35.0 37.0 7 36.230097266273646 37.0 37.0 37.0 35.0 37.0 8 36.2791536602146 37.0 37.0 37.0 35.0 37.0 9 38.099944067258846 39.0 39.0 39.0 37.0 39.0 10-11 38.09092090587053 39.0 39.0 39.0 36.0 39.0 12-13 38.083025557882756 39.0 39.0 39.0 35.0 39.0 14-15 39.57624581185883 41.0 40.0 41.0 37.0 41.0 16-17 39.59442646043876 41.0 40.0 41.0 37.0 41.0 18-19 39.67291786827567 41.0 40.0 41.0 37.0 41.0 20-21 39.67686772288325 41.0 40.0 41.0 37.0 41.0 22-23 39.610934251226325 41.0 40.0 41.0 37.0 41.0 24-25 39.59579741226574 41.0 40.0 41.0 37.0 41.0 26-27 39.51534159858609 41.0 40.0 41.0 36.5 41.0 28-29 39.4325330117655 41.0 39.5 41.0 36.0 41.0 30-31 39.26777510894891 41.0 39.0 41.0 35.5 41.0 32-33 39.078109691410454 41.0 39.0 41.0 35.0 41.0 34-35 38.981548846291815 41.0 39.0 41.0 35.0 41.0 36-37 38.876034619422946 41.0 39.0 41.0 35.0 41.0 38-39 38.7571440720562 41.0 39.0 41.0 35.0 41.0 40-41 38.66180862280071 41.0 39.0 41.0 35.0 41.0 42-43 38.53492503595287 41.0 38.5 41.0 35.0 41.0 44-45 38.337512225065495 41.0 38.0 41.0 35.0 41.0 46-47 38.04221548170302 41.0 38.0 41.0 34.0 41.0 48-49 37.78581478085377 40.5 38.0 41.0 33.0 41.0 50-51 37.64433689173144 40.0 38.0 41.0 33.0 41.0 52-53 37.541909541602294 40.0 38.0 41.0 33.0 41.0 54-55 37.40831260841739 40.0 37.5 41.0 33.0 41.0 56-57 37.259424121730575 40.0 37.0 41.0 33.0 41.0 58-59 37.090134249481835 40.0 37.0 41.0 32.5 41.0 60-61 36.90600213918203 40.0 36.0 41.0 32.0 41.0 62-63 36.69106340897519 39.5 36.0 41.0 32.0 41.0 64-65 36.43464362775616 39.0 35.5 41.0 31.5 41.0 66-67 36.1563876171671 39.0 35.0 41.0 31.0 41.0 68-69 35.8849694659568 38.5 35.0 41.0 31.0 41.0 70-71 35.62640112606891 37.5 35.0 40.0 31.0 41.0 72-73 35.35568207960766 37.0 35.0 40.0 31.0 41.0 74-75 35.04416723998635 36.5 35.0 39.0 31.0 41.0 76-77 34.70595547404906 36.0 35.0 39.0 31.0 41.0 78-79 34.36534721366634 36.0 35.0 38.5 30.0 40.0 80-81 34.02821725018072 35.0 35.0 37.0 30.0 39.0 82-83 33.68462122195049 35.0 35.0 37.0 30.0 39.0 84-85 33.40405779498524 35.0 34.5 36.5 30.0 38.5 86-87 33.15921096674237 35.0 34.0 36.0 30.0 37.0 88-89 32.91956021383098 35.0 34.0 36.0 29.0 37.0 90-91 32.7097325150217 35.0 34.0 35.5 29.0 36.5 92-93 32.55328961929756 35.0 34.0 35.0 29.0 36.0 94-95 32.415086357753665 35.0 34.0 35.0 29.0 36.0 96-97 32.27913272632317 35.0 34.0 35.0 29.0 36.0 98-99 32.160493494684204 35.0 34.0 35.0 29.0 36.0 100 32.05878062263063 35.0 34.0 35.0 29.0 36.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.23482731938629797 1101 2 0.23261081105886205 1101 3 0.24771738265810228 1101 4 0.12595586702911987 1101 5 0.19296798398071502 1101 6 0.2289887455396311 1101 7 0.2446910637784825 1101 8 0.27982027712573654 1101 9 0.3125622220957993 1101 10-11 0.3374299008720598 1101 12-13 0.30026094903707445 1101 14-15 0.41091942110129054 1101 16-17 0.40656744984365645 1101 18-19 0.4009660955985481 1101 20-21 0.3783863477532492 1101 22-23 0.4141807706456859 1101 24-25 0.363618586784213 1101 26-27 0.37717517281480184 1101 28-29 0.3708932810858414 1101 30-31 0.43974437850826575 1101 32-33 0.4439805036174178 1101 34-35 0.44196821023852806 1101 36-37 0.4403986694678039 1101 38-39 0.4324035145986116 1101 40-41 0.4599990886441603 1101 42-43 0.5100917547252308 1101 44-45 0.49169713110581625 1101 46-47 0.5188576139605985 1101 48-49 0.5026491889739617 1101 50-51 0.49542741614014574 1101 52-53 0.45023097618915386 1101 54-55 0.5089408430895048 1101 56-57 0.5080300883173123 1101 58-59 0.5260743626827775 1101 60-61 0.5114428656310395 1101 62-63 0.45750003653708404 1101 64-65 0.4964869305295778 1101 66-67 0.5305697476557967 1101 68-69 0.5071389979305394 1101 70-71 0.44497786009249296 1101 72-73 0.4286098009124757 1101 74-75 0.4256372494572389 1101 76-77 0.4352834436804258 1101 78-79 0.39399433577821696 1101 80-81 0.39782499995747145 1101 82-83 0.44943061294705444 1101 84-85 0.4017922330404886 1101 86-87 0.39040605224580816 1101 88-89 0.4006587474408363 1101 90-91 0.4003236497726306 1101 92-93 0.4736180267350747 1101 94-95 0.4123346649028079 1101 96-97 0.42546446815615013 1101 98-99 0.454284614023031 1101 100 0.5080618351813513 1102 1 0.19328136996021783 1102 2 0.22512692048211136 1102 3 0.20528150226439834 1102 4 0.11249230232619567 1102 5 0.1533380287014765 1102 6 0.12976829374795784 1102 7 0.191333998952679 1102 8 0.21392249942626052 1102 9 0.24780635341836188 1102 10-11 0.2280172392951414 1102 12-13 0.22698738575241606 1102 14-15 0.32151125455661145 1102 16-17 0.3352212695444692 1102 18-19 0.30283941719070384 1102 20-21 0.294176563210506 1102 22-23 0.33093352091674433 1102 24-25 0.33048605291175903 1102 26-27 0.3629400142955177 1102 28-29 0.3050601884307014 1102 30-31 0.31767328973351283 1102 32-33 0.34982514882572957 1102 34-35 0.3368919379406563 1102 36-37 0.3535565506242051 1102 38-39 0.3034574822119609 1102 40-41 0.35487183349840024 1102 42-43 0.38491889795785283 1102 44-45 0.3659390837791321 1102 46-47 0.4109236329226533 1102 48-49 0.38122406088957206 1102 50-51 0.3355501878204521 1102 52-53 0.3434737824915288 1102 54-55 0.3460742225832192 1102 56-57 0.3503261707169898 1102 58-59 0.39670516376124 1102 60-61 0.33579752331903023 1102 62-63 0.2999960464396807 1102 64-65 0.2868595181644622 1102 66-67 0.34817491690727564 1102 68-69 0.3695667210685869 1102 70-71 0.4058244565291744 1102 72-73 0.3758286732145919 1102 74-75 0.326330847524126 1102 76-77 0.3240003433111198 1102 78-79 0.3427039892603432 1102 80-81 0.2942878224284371 1102 82-83 0.3381936907593328 1102 84-85 0.29890920192956116 1102 86-87 0.30596457601742344 1102 88-89 0.25238576694439274 1102 90-91 0.3140952825601744 1102 92-93 0.2926018682864324 1102 94-95 0.3251051475955933 1102 96-97 0.36049425797691015 1102 98-99 0.355870011538876 1102 100 0.40416857849490384 1103 1 0.1751023835551706 1103 2 0.21024020941097632 1103 3 0.18214594983003707 1103 4 0.13675088704243166 1103 5 0.13681596654551242 1103 6 0.18132464846549823 1103 7 0.1652220514186169 1103 8 0.1908818338362579 1103 9 0.22888019888575428 1103 10-11 0.24284466801249494 1103 12-13 0.2189987377969871 1103 14-15 0.3311443739589848 1103 16-17 0.3359725593741416 1103 18-19 0.32201532933215304 1103 20-21 0.3233224342822538 1103 22-23 0.3216155801822609 1103 24-25 0.29198347956651105 1103 26-27 0.2933863788490214 1103 28-29 0.3108708237551241 1103 30-31 0.36310514589557386 1103 32-33 0.34337380806234563 1103 34-35 0.30811377531478 1103 36-37 0.2703169348580374 1103 38-39 0.29339570578991925 1103 40-41 0.26420280582722455 1103 42-43 0.28067412357456334 1103 44-45 0.317154968363063 1103 46-47 0.290466099433381 1103 48-49 0.1920966024257993 1103 50-51 0.20456942266416434 1103 52-53 0.18924600355226318 1103 54-55 0.21021143281880228 1103 56-57 0.227668174474573 1103 58-59 0.21907199244294873 1103 60-61 0.15035188488666051 1103 62-63 0.20370089538543823 1103 64-65 0.24300145064292167 1103 66-67 0.2570831078343403 1103 68-69 0.20124158086055388 1103 70-71 0.17714874012472848 1103 72-73 0.18135826107243957 1103 74-75 0.12515951240133205 1103 76-77 0.14039228245444946 1103 78-79 0.11489593499840112 1103 80-81 0.13429597462904042 1103 82-83 0.15896875316366277 1103 84-85 0.12144326648208192 1103 86-87 0.11595217934060287 1103 88-89 0.14970188992055 1103 90-91 0.20192842132578903 1103 92-93 0.21215001397650468 1103 94-95 0.17571116058018532 1103 96-97 0.15406003543165525 1103 98-99 0.1418539618062482 1103 100 0.16336663908497684 1104 1 0.11987261263471538 1104 2 0.14125784014860443 1104 3 0.1675481306138522 1104 4 0.051139889218354995 1104 5 0.09634318227778493 1104 6 0.07798376575860289 1104 7 0.07076605456701657 1104 8 0.11588946931472321 1104 9 0.19145989656639273 1104 10-11 0.1782587768571915 1104 12-13 0.16873915052308774 1104 14-15 0.23087677133207762 1104 16-17 0.24891319485868024 1104 18-19 0.20036449330424233 1104 20-21 0.19094954410550713 1104 22-23 0.22732910234870474 1104 24-25 0.2550234902333841 1104 26-27 0.18745741083142065 1104 28-29 0.1967019968685193 1104 30-31 0.2462088197383281 1104 32-33 0.21096293043057557 1104 34-35 0.205665787738198 1104 36-37 0.23946428363758088 1104 38-39 0.2839895370830732 1104 40-41 0.2536347908085048 1104 42-43 0.17817979130285266 1104 44-45 0.21937706353835296 1104 46-47 0.24807314428289828 1104 48-49 0.28973699266332886 1104 50-51 0.2643730731066398 1104 52-53 0.2651539607084956 1104 54-55 0.2860984968747644 1104 56-57 0.2985916077199775 1104 58-59 0.2513698763897523 1104 60-61 0.27627013664320543 1104 62-63 0.3116064456565155 1104 64-65 0.27123942533415857 1104 66-67 0.2801252533961929 1104 68-69 0.23857242272333679 1104 70-71 0.25754852390013383 1104 72-73 0.27100409643177414 1104 74-75 0.3064292848716761 1104 76-77 0.22261840809871813 1104 78-79 0.20617333155095707 1104 80-81 0.20197214525045837 1104 82-83 0.22320430501501676 1104 84-85 0.25982130851748053 1104 86-87 0.25423496783253086 1104 88-89 0.2834571435855864 1104 90-91 0.2703798209355739 1104 92-93 0.28319633663890187 1104 94-95 0.2154336926112137 1104 96-97 0.25218381603434636 1104 98-99 0.23560291715123327 1104 100 0.25326756020479024 1105 1 0.0027601619812003264 1105 2 0.06521037042149658 1105 3 0.10287212464088924 1105 4 -0.0991515516233008 1105 5 -0.06383191271229549 1105 6 -0.06830753074648754 1105 7 -0.4114938196410236 1105 8 -0.37178784362206585 1105 9 -0.3148054250245451 1105 10-11 -0.3268843468279101 1105 12-13 -0.26192647058511653 1105 14-15 -0.18849824472631127 1105 16-17 -0.1540155763686215 1105 18-19 -0.1627225918981665 1105 20-21 -0.08395453536726194 1105 22-23 -0.12169081175214558 1105 24-25 -0.1351314949324447 1105 26-27 -0.143522817703996 1105 28-29 -0.18370245345854386 1105 30-31 -0.200177466852125 1105 32-33 -0.24719304430977473 1105 34-35 -0.200034543102376 1105 36-37 -0.13395273719525136 1105 38-39 -0.18454719336121173 1105 40-41 -0.15295326186451774 1105 42-43 -0.09697397248091733 1105 44-45 -0.16842659584700215 1105 46-47 -0.17401177190365757 1105 48-49 -0.21069013683000293 1105 50-51 -0.22298595314296676 1105 52-53 -0.24139722379595696 1105 54-55 -0.29536591566030523 1105 56-57 -0.2739787724141749 1105 58-59 -0.2724985079324256 1105 60-61 -0.23496863287624592 1105 62-63 -0.2649585528396585 1105 64-65 -0.1905050558703465 1105 66-67 -0.2531977663590226 1105 68-69 -0.19930112370920483 1105 70-71 -0.2695269329515426 1105 72-73 -0.23925929135145907 1105 74-75 -0.2726622175208391 1105 76-77 -0.2755898536960686 1105 78-79 -0.28937348866159596 1105 80-81 -0.240914215775355 1105 82-83 -0.21437997790429364 1105 84-85 -0.1939557564778056 1105 86-87 -0.2227581567216106 1105 88-89 -0.20929591865445474 1105 90-91 -0.2232960318214765 1105 92-93 -0.24059721087427022 1105 94-95 -0.24521253657711384 1105 96-97 -0.24780402209862018 1105 98-99 -0.2032132802452722 1105 100 -0.1834252224379469 1106 1 0.09487989448065548 1106 2 0.09641414036823193 1106 3 0.11057563757763944 1106 4 0.05773548395265493 1106 5 0.08374952267311642 1106 6 0.10066654846162493 1106 7 0.09287013976628344 1106 8 0.09650929669312092 1106 9 0.1053565043979745 1106 10-11 0.12709083572798363 1106 12-13 0.12369093131106723 1106 14-15 0.14513617412026747 1106 16-17 0.19202511264580124 1106 18-19 0.1587674924203739 1106 20-21 0.12228253203939943 1106 22-23 0.1681598772710231 1106 24-25 0.15674974806764652 1106 26-27 0.13480824179885076 1106 28-29 0.10189629660604282 1106 30-31 0.1519729448454683 1106 32-33 0.1135575693179689 1106 34-35 0.16846794249499908 1106 36-37 0.1550702001792601 1106 38-39 0.19652875024195282 1106 40-41 0.13243252456413046 1106 42-43 0.14130904323128135 1106 44-45 0.0828727931960529 1106 46-47 0.09124030365990876 1106 48-49 0.1419904728834922 1106 50-51 0.139185921296054 1106 52-53 0.10263743008121651 1106 54-55 0.06941758736383719 1106 56-57 0.08553663899007091 1106 58-59 0.05862820280393777 1106 60-61 0.014776830919501549 1106 62-63 0.08598861869326413 1106 64-65 0.09305031161014199 1106 66-67 0.10547038778391737 1106 68-69 0.07275031068670046 1106 70-71 0.05953634229257432 1106 72-73 0.13388375681318365 1106 74-75 0.1278817285587266 1106 76-77 0.10547275778802856 1106 78-79 0.07923033417577585 1106 80-81 0.09020568660464079 1106 82-83 0.11894572248073843 1106 84-85 0.133234870495194 1106 86-87 0.09659434450652071 1106 88-89 0.1245202679829518 1106 90-91 0.06378803452300019 1106 92-93 0.14821455799363292 1106 94-95 0.0722746939236174 1106 96-97 0.07189723773190337 1106 98-99 0.059383207956351214 1106 100 0.06423125811029706 1107 1 -0.033859115374490045 1107 2 0.005348121066084843 1107 3 0.014672583866996547 1107 4 -0.07382536409892992 1107 5 -0.10800419452016996 1107 6 -0.059895260585129506 1107 7 -0.04168776438091726 1107 8 -0.004499900533240009 1107 9 -0.007261392652182508 1107 10-11 0.014635744357292424 1107 12-13 0.04815473103419521 1107 14-15 0.04705412858646696 1107 16-17 0.05613921521185716 1107 18-19 0.07451258350169354 1107 20-21 0.048944246509613265 1107 22-23 0.044131571318189344 1107 24-25 0.07004525332114042 1107 26-27 0.07297136273346894 1107 28-29 0.02788314422205218 1107 30-31 0.07430728740141035 1107 32-33 0.10856983685537358 1107 34-35 0.11962067980826419 1107 36-37 0.09661188163143919 1107 38-39 0.0848533823300528 1107 40-41 0.07524733424596519 1107 42-43 0.11206695773469022 1107 44-45 0.13300711930390463 1107 46-47 0.2165221295811861 1107 48-49 0.20653359658991377 1107 50-51 0.20444876337454332 1107 52-53 0.17860379372727664 1107 54-55 0.15323601258059227 1107 56-57 0.18564850126468713 1107 58-59 0.1821703381016988 1107 60-61 0.17829537538494833 1107 62-63 0.19274958523205044 1107 64-65 0.22905629536612082 1107 66-67 0.2729788136250946 1107 68-69 0.2534452350787717 1107 70-71 0.26818762136039 1107 72-73 0.27153835748237753 1107 74-75 0.24917237857830088 1107 76-77 0.22894674010023408 1107 78-79 0.2170108465339311 1107 80-81 0.2528146742700912 1107 82-83 0.2519370598174575 1107 84-85 0.23255444479413967 1107 86-87 0.1953685426238252 1107 88-89 0.20682596344037307 1107 90-91 0.2557201297818352 1107 92-93 0.25061803585190745 1107 94-95 0.21181831259585948 1107 96-97 0.25267055709451114 1107 98-99 0.21765888411773204 1107 100 0.2399178518469185 1108 1 -0.09448803346967338 1108 2 -0.06483568006476759 1108 3 -0.05304098014951819 1108 4 -0.02665410764281262 1108 5 -0.20787585148007537 1108 6 -0.17591596271701349 1108 7 -0.19624275566488336 1108 8 -0.09726974436034652 1108 9 -0.1676620519326164 1108 10-11 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98-99 -0.34295882945110634 2214 100 -0.3646318417811685 2215 1 -0.2441355991967029 2215 2 -0.2310901863372905 2215 3 -0.23252472309282268 2215 4 -0.1410276812750979 2215 5 -0.14571509282991002 2215 6 -0.2254244393122704 2215 7 -0.2743983028911643 2215 8 -0.23073377843058296 2215 9 -0.28353029620386394 2215 10-11 -0.3185777429239067 2215 12-13 -0.2856524591287055 2215 14-15 -0.40008687497963535 2215 16-17 -0.35983948526521914 2215 18-19 -0.4250834850869438 2215 20-21 -0.3814821570270581 2215 22-23 -0.3800632786865634 2215 24-25 -0.3973606135889298 2215 26-27 -0.37286578075396193 2215 28-29 -0.3574128031677759 2215 30-31 -0.4086008036225337 2215 32-33 -0.45917502052408565 2215 34-35 -0.40908469350546284 2215 36-37 -0.42090067577546364 2215 38-39 -0.47526201450518357 2215 40-41 -0.47175355658655604 2215 42-43 -0.4185324294394306 2215 44-45 -0.3560565357342398 2215 46-47 -0.4383453084164515 2215 48-49 -0.4381848149536651 2215 50-51 -0.4352258460373122 2215 52-53 -0.4790170292687037 2215 54-55 -0.5141495730357377 2215 56-57 -0.5244800837224872 2215 58-59 -0.5180268114500564 2215 60-61 -0.4412205739161692 2215 62-63 -0.47141718591247184 2215 64-65 -0.44026890287935316 2215 66-67 -0.4277749797979382 2215 68-69 -0.47280745894943266 2215 70-71 -0.4931774649918239 2215 72-73 -0.45866639843969637 2215 74-75 -0.5038921554226832 2215 76-77 -0.3721367773535462 2215 78-79 -0.4842378145027766 2215 80-81 -0.4933304215009784 2215 82-83 -0.44357767196535036 2215 84-85 -0.39221599335127877 2215 86-87 -0.4275737718398531 2215 88-89 -0.4244568960827664 2215 90-91 -0.5110846983159476 2215 92-93 -0.5017398900520575 2215 94-95 -0.49020595744785567 2215 96-97 -0.5691424961477871 2215 98-99 -0.6544762082158542 2215 100 -0.5958632027102126 2216 1 -0.42317780618308376 2216 2 -0.38177438064170843 2216 3 -0.5037148742821103 2216 4 -0.2701522483772365 2216 5 -0.40501511094240783 2216 6 -0.3246749925386325 2216 7 -0.466093021954606 2216 8 -0.43424956587107744 2216 9 -0.4690107691875127 2216 10-11 -0.49899696615456435 2216 12-13 -0.5226066498403199 2216 14-15 -0.6483457844307452 2216 16-17 -0.7329054199433642 2216 18-19 -0.701269369346015 2216 20-21 -0.7004833070038003 2216 22-23 -0.6981428502458726 2216 24-25 -0.768096457619535 2216 26-27 -0.8008877316862879 2216 28-29 -0.7586448976159375 2216 30-31 -0.8529214533252514 2216 32-33 -0.8782223098134665 2216 34-35 -0.8308574801718152 2216 36-37 -0.8572310245307193 2216 38-39 -0.8968189425308211 2216 40-41 -0.9672336435661393 2216 42-43 -0.9096828207000982 2216 44-45 -0.9426155906406066 2216 46-47 -0.9357117826732662 2216 48-49 -0.9198923657585354 2216 50-51 -0.8671899368892824 2216 52-53 -0.8945193971209875 2216 54-55 -0.9437120089921578 2216 56-57 -1.0199151368943333 2216 58-59 -1.1020746842749105 2216 60-61 -1.0069683059829089 2216 62-63 -1.064225740594651 2216 64-65 -1.0075969617632055 2216 66-67 -0.8710922453974703 2216 68-69 -0.8806577784831262 2216 70-71 -0.8998602354595917 2216 72-73 -1.0387463390262752 2216 74-75 -0.9419255185543207 2216 76-77 -0.8697068725298038 2216 78-79 -0.8543588115957377 2216 80-81 -0.8523197809910599 2216 82-83 -0.924767547868818 2216 84-85 -0.9397374387625419 2216 86-87 -0.8705540765233764 2216 88-89 -0.8153230283124699 2216 90-91 -0.8087591739572701 2216 92-93 -0.9572857929896337 2216 94-95 -0.9075400748005755 2216 96-97 -1.050162780216919 2216 98-99 -1.0608213719111887 2216 100 -1.0588638574073315 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 72.0 3 401.0 4 203.0 5 313.0 6 382.0 7 680.0 8 1299.0 9 1899.0 10 2870.0 11 4569.0 12 7473.0 13 11384.0 14 11837.0 15 15531.0 16 20144.0 17 31907.0 18 38233.0 19 18914.0 20 11237.0 21 10062.0 22 10154.0 23 10383.0 24 11717.0 25 13976.0 26 17330.0 27 21846.0 28 26885.0 29 33756.0 30 42819.0 31 53855.0 32 67677.0 33 87734.0 34 122121.0 35 191661.0 36 340333.0 37 785850.0 38 1574864.0 39 972031.0 40 11463.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.877588650533454 22.41320315633213 15.162113895498566 23.547094297635848 2 13.046829769301974 22.87278408762578 41.12271076449045 22.957675378581794 3 14.528411205863954 29.821937006648056 32.830191312448356 22.819460475039627 4 10.09874286621444 19.478889926160374 41.72408912916952 28.69827807845567 5 11.04179909351889 39.29655582970715 36.76584461164906 12.895800465124902 6 26.309976416662938 41.75358411117641 19.23591296298517 12.700526509175477 7 23.180446873163515 34.98194997018011 25.49619319365049 16.34140996300589 8 23.83587393000012 35.64919595321712 22.39631999633657 18.118610120446196 9 25.291128282232467 15.924302176361493 22.911424562214545 35.873144979191494 10-11 22.145625743452978 27.69974693978126 30.85541113835667 19.29921617840909 12-13 23.701406386799437 26.23653334758001 30.586388827407696 19.475671438212856 14-15 21.18055154262064 26.94535273061898 29.1232951689594 22.750800557800982 16-17 19.51926190587817 30.371881858711497 29.14409822356306 20.964758011847273 18-19 19.354614669206356 28.496466860668594 32.75674273010654 19.392175740018512 20-21 20.89698453835863 27.624821053389052 32.29122531954168 19.186969088710637 22-23 20.25243901907167 27.82666719001452 32.369163035940915 19.551730754972894 24-25 20.05494496508453 27.925919699057637 32.12228498396648 19.896850351891352 26-27 19.93074985087242 27.950624515015082 32.15807752497171 19.96054810914078 28-29 19.98670466351863 27.756320927223786 32.437524511709306 19.819449897548278 30-31 20.389110836664184 27.978972427533456 31.9807269785774 19.65118975722496 32-33 19.774085406475955 27.908303931965463 32.399053486392724 19.91855717516586 34-35 20.23486061352532 27.679186220120823 32.076667604018525 20.009285562335336 36-37 20.541887331127697 27.97389872573872 31.46270784692291 20.02150609621067 38-39 20.335986296687402 27.95405988439193 31.640434149030067 20.0695196698906 40-41 20.251176675124487 27.887606604580135 31.670336229185686 20.19088049110969 42-43 20.213169257231964 27.98079828131796 31.57390266275128 20.232129798698796 44-45 20.44355213284714 27.865591531500648 31.217898479712126 20.472957855940088 46-47 20.71805566125848 28.20224074694763 30.667053666580674 20.41264992521321 48-49 20.751884585249503 28.11715710186999 30.365313731189858 20.76564458169066 50-51 20.914525646449274 28.299856950338214 29.807275470908145 20.978341932304364 52-53 20.700164603642346 28.463597212695664 29.973050882772483 20.863187300889503 54-55 20.96928173884866 28.59567246037203 29.727755379113834 20.707290421665476 56-57 20.92004799135423 28.42146649262314 29.810234841007922 20.84825067501471 58-59 20.781402541873 27.98945272950529 30.086324594168428 21.142820134453274 60-61 21.099098912469287 28.352294750316055 29.70884880057612 20.83975753663854 62-63 20.666777151093633 28.277369700154715 30.111472971838467 20.94438017691319 64-65 20.7311706733626 28.10213558401741 30.134325803310823 21.03236793930916 66-67 20.803114293770168 28.177712048633364 29.870472541133836 21.14870111646263 68-69 21.018221236044376 28.26820572631282 29.744972039415764 20.96860099822704 70-71 21.065325907739197 28.150573045564304 29.72069412252405 21.063406924172455 72-73 20.81256523449966 28.22857961522807 29.863060566944977 21.09579458332729 74-75 20.86668238920165 28.10776020495556 29.767741523269407 21.25781588257338 76-77 20.92419705742729 28.394173731331435 29.525381439562693 21.156247771678583 78-79 20.919632618842975 28.43280995427307 29.582563340234696 21.06499408664926 80-81 20.95186746557737 28.315674760989157 29.560531877834478 21.171925895598996 82-83 20.677638709788564 28.321081580742668 29.812345985379018 21.188933724089754 84-85 20.785849493039894 28.066998785057702 29.58845151717323 21.55870020472917 86-87 20.83452313392112 28.345067792738643 29.492826033981682 21.32758303935855 88-89 20.544713065282856 28.157773452581402 29.6922526225304 21.60526085960534 90-91 20.626041739241554 28.16183635479282 29.528016005127157 21.68410590083847 92-93 20.587362379874396 28.237457097804246 29.66185688139335 21.513323640928004 94-95 20.562610578678594 27.812439589573813 29.736621717584335 21.888328114163265 96-97 20.458711265012642 28.15312749106719 29.525844186585847 21.86231705733433 98-99 20.23022148137778 28.01964255418624 29.689093929509582 22.061042034926395 100 20.442261717456066 27.995109144708735 29.41681108297292 22.145818054862275 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1160.0 1 780.0 2 1041.5 3 1537.0 4 959.5 5 760.0 6 1565.5 7 4098.0 8 7719.5 9 8526.5 10 5793.0 11 3597.0 12 3357.5 13 3606.5 14 3947.5 15 4325.5 16 4858.0 17 5524.0 18 6274.0 19 7187.0 20 8435.0 21 10340.0 22 13305.5 23 17559.5 24 23370.0 25 31082.5 26 41253.5 27 54135.5 28 69537.0 29 85710.5 30 100603.5 31 114727.5 32 127727.0 33 137839.5 34 146929.0 35 155852.5 36 163343.0 37 169795.5 38 175933.5 39 179818.5 40 182000.0 41 182236.5 42 177787.5 43 173761.0 44 169648.0 45 160741.5 46 152445.0 47 145070.5 48 133830.5 49 127260.5 50 125077.5 51 118524.5 52 108466.0 53 99122.5 54 90588.0 55 82370.5 56 73322.5 57 63373.5 58 56385.0 59 51156.5 60 44440.0 61 36595.0 62 29944.5 63 23795.5 64 18378.0 65 14032.0 66 10666.5 67 8482.0 68 6410.0 69 4481.0 70 3592.0 71 3284.5 72 2323.0 73 1107.5 74 628.0 75 435.5 76 259.0 77 171.0 78 122.0 79 65.5 80 30.5 81 12.0 82 7.5 83 6.5 84 9.0 85 7.5 86 5.0 87 4.0 88 3.0 89 2.5 90 3.0 91 3.0 92 2.5 93 3.0 94 7.0 95 11.0 96 12.5 97 11.0 98 6.5 99 2.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.00907135295086096 2 0.0 3 3.4889819041772927E-4 4 0.002529511880528537 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.0903068450554039E-4 24-25 7.632147915387828E-5 26-27 5.778626278793641E-4 28-29 0.0010139853659015257 30-31 0.0016681694729347681 32-33 0.004230390558814967 34-35 0.005484243430628682 36-37 0.004110456805858873 38-39 0.002453190401374659 40-41 0.003674334067836711 42-43 5.560564909782561E-4 44-45 5.015411487254858E-4 46-47 0.0035325941779795083 48-49 0.002355062785319673 50-51 9.812761605498637E-4 52-53 1.090306845055404E-5 54-55 5.6695955942881E-4 56-57 8.50439339143215E-4 58-59 7.414086546376746E-4 60-61 3.816073957693914E-4 62-63 0.0 64-65 0.0 66-67 9.158577498465392E-4 68-69 7.523117230882287E-4 70-71 0.00226783823771524 72-73 1.1993375295609444E-4 74-75 0.001973455389550281 76-77 3.052859166155131E-4 78-79 0.0012102405980114984 80-81 8.177301337915528E-4 82-83 0.0012756590087148227 84-85 0.0011012099135059579 86-87 2.72576711263851E-4 88-89 0.0016245571991325518 90-91 0.0018426185681436327 92-93 0.003140083713759563 94-95 0.0010139853659015257 96-97 0.002278741306165794 98-99 0.002333256648418564 100 0.003576206451781725 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4585865.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.63702817407851 #Duplication Level Percentage of deduplicated Percentage of total 1 75.3187286788305 44.9178514425519 2 15.547680354346236 18.54434902667427 3 4.533219063856197 8.110431390913856 4 1.7609659211941437 4.20075097023389 5 0.8877781685891203 2.64722258262406 6 0.4984455384503221 1.7835486371883356 7 0.2964426863508082 1.237527258653187 8 0.20888248303931098 0.9965704420869492 9 0.15426109811135824 0.8279706108808226 >10 0.7055589885406555 7.570140150810039 >50 0.050690657199046946 2.0993757539648383 >100 0.03310120502540846 3.7875419803236476 >500 0.003220464095789693 1.3084011104780346 >1k 9.149039028045918E-4 0.8415884238083626 >5k 0.0 0.0 >10k+ 1.0978846833628304E-4 1.1267302188081034 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23543 0.5133818810627875 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16100 0.35107880410784004 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11983 0.2613029384859781 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.180613690110808E-5 0.0 4 0.0 0.0 0.0 2.180613690110808E-5 0.0 5 0.0 0.0 0.0 2.180613690110808E-5 0.0 6 0.0 0.0 0.0 4.361227380221616E-5 0.0 7 0.0 0.0 0.0 4.361227380221616E-5 0.0 8 0.0 0.0 0.0 1.5264295830775656E-4 0.0 9 0.0 0.0 0.0 1.962552321099727E-4 0.0 10-11 0.0 0.0 0.0 2.1806136901108078E-4 0.0 12-13 0.0 0.0 0.0 2.3986750591218888E-4 0.0 14-15 0.0 0.0 0.0 2.507705743627429E-4 0.0 16-17 0.0 0.0 0.0 2.616736428132969E-4 0.0 18-19 0.0 0.0 0.0 2.616736428132969E-4 0.0 20-21 0.0 0.0 0.0 3.379951219671752E-4 0.0 22-23 0.0 0.0 0.0 6.650871754837964E-4 0.0 24-25 0.0 0.0 0.0 0.0011448221873081742 0.0 26-27 0.0 0.0 0.0 0.0018099093627919707 0.0 28-29 0.0 0.0 0.0 0.0039360077106500085 0.0 30-31 0.0 0.0 0.0 0.008526199528333259 0.0 32-33 0.0 0.0 0.0 0.01761935861609533 0.0 34-35 0.0 0.0 0.0 0.029787183006913635 0.0 36-37 0.0 0.0 0.0 0.0480389195931411 0.0 38-39 0.0 0.0 0.0 0.07469692195474573 0.0 40-41 0.0 0.0 0.0 0.1055417026013631 0.0 42-43 0.0 0.0 0.0 0.13484915059645236 0.0 44-45 0.0 0.0 0.0 0.16993522487033524 0.0 46-47 0.0 0.0 0.0 0.21114882361342951 0.0 48-49 0.0 0.0 0.0 0.25727970622772367 0.0 50-51 0.0 0.0 0.0 0.3082079389602616 0.0 52-53 0.0 0.0 0.0 0.35759883904127143 0.0 54-55 0.0 0.0 0.0 0.4085270717738093 0.0 56-57 1.090306845055404E-5 0.0 0.0 0.4717430626501216 0.0 58-59 2.180613690110808E-5 0.0 0.0 0.543223579411954 0.0 60-61 2.180613690110808E-5 0.0 0.0 0.6225979177319874 0.0 62-63 2.180613690110808E-5 0.0 0.0 0.7073583718665943 0.0 64-65 2.180613690110808E-5 0.0 0.0 0.7956405171107305 0.0 66-67 2.180613690110808E-5 0.0 0.0 0.8968537015372237 0.0 68-69 4.361227380221616E-5 0.0 0.0 1.0126224823452064 0.0 70-71 4.361227380221616E-5 0.0 0.0 1.1460106217692845 0.0 72-73 4.361227380221616E-5 0.0 0.0 1.2885900478971797 0.0 74-75 4.361227380221616E-5 0.0 0.0 1.4344512976286916 0.0 76-77 4.361227380221616E-5 0.0 0.0 1.5907249777304826 0.0 78-79 4.361227380221616E-5 0.0 0.0 1.769339045087459 0.0 80-81 4.361227380221616E-5 0.0 0.0 1.9716890924612915 0.0 82-83 4.361227380221616E-5 0.0 0.0 2.1937845967990772 0.0 84-85 4.361227380221616E-5 0.0 0.0 2.42074286966581 0.0 86-87 4.361227380221616E-5 0.0 0.0 2.6639140053185164 0.0 88 4.361227380221616E-5 0.0 0.0 2.8582175881758403 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8310 0.0 62.956455 1 GTATCAA 18375 0.0 50.08774 1 ATCAACG 24390 0.0 37.538002 3 TCAACGC 24410 0.0 37.507248 4 CAACGCA 25310 0.0 36.19091 5 TATCAAC 25980 0.0 35.313007 2 AACGCAG 26090 0.0 35.180984 6 ACGCAGA 28990 0.0 31.580612 7 CGCAGAG 29105 0.0 31.294353 8 GCAGAGT 31540 0.0 28.595198 9 GTGGTAT 2990 0.0 25.310505 1 GTACATG 17970 0.0 24.19579 1 TACATGG 17260 0.0 24.15332 2 AGAGTAC 30040 0.0 23.835396 10-11 TGGTATC 3140 0.0 23.799152 2 GAGTACT 21440 0.0 22.830442 12-13 ACATGGG 17865 0.0 22.519814 3 AGTACTT 22770 0.0 22.405085 12-13 CAGAGTA 31195 0.0 22.161928 10-11 GTACTTT 24560 0.0 19.671822 14-15 >>END_MODULE