FastQCFastQC Report
Wed 25 May 2016
SRR2431370_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431370_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4558867
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA350500.7688313784982103No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52400.040.09321
GTATCAA93700.035.71371
TCAACGC98750.031.9835474
ATCAACG99600.031.8049743
CAACGCA104550.030.2541855
AACGCAG110000.028.9261386
ACGCAGA121550.026.0228317
CGCAGAG120250.025.9523948
GTACATG176250.025.8664651
TACATGG168500.025.661522
TATCAAC129800.025.1292342
ACATGGG173350.023.4252573
GCAGAGT137450.021.3370489
CATGGGG86500.020.2125594
CATGGGT42750.019.2396164
AGAGTAC130550.017.13659710-11
CATGGGA57550.016.496834
CAGAGTA141650.016.27484510-11
CTGTCGC10050.015.90037259
TCTGTCG9400.015.4998788
Produced by FastQC (version 0.11.5)