##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431370_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4558867
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.18296190698259	33.0	31.0	34.0	28.0	34.0
2	31.245932816201922	34.0	31.0	34.0	28.0	34.0
3	31.351339707870398	34.0	31.0	34.0	28.0	34.0
4	34.54386473656722	37.0	35.0	37.0	32.0	37.0
5	34.69503848214919	37.0	35.0	37.0	32.0	37.0
6	34.70498459375981	37.0	35.0	37.0	32.0	37.0
7	34.628395827296565	37.0	35.0	37.0	32.0	37.0
8	34.56607003450638	37.0	35.0	37.0	31.0	37.0
9	36.06065673773769	39.0	37.0	39.0	31.0	39.0
10-11	36.095150176568	39.0	37.0	39.0	31.0	39.0
12-13	36.074268343428315	39.0	37.0	39.0	31.0	39.0
14-15	37.473231726216184	41.0	38.0	41.0	31.0	41.0
16-17	37.49083412611072	41.0	38.0	41.0	31.0	41.0
18-19	37.5143598179109	41.0	38.5	41.0	31.0	41.0
20-21	37.562728743786565	41.0	38.5	41.0	31.0	41.0
22-23	37.57026175582661	41.0	38.0	41.0	31.0	41.0
24-25	37.595153357182824	41.0	38.0	41.0	31.0	41.0
26-27	37.59679126414524	41.0	38.0	41.0	31.0	41.0
28-29	37.57034313569578	41.0	38.0	41.0	30.5	41.0
30-31	37.50406877410549	40.5	38.0	41.0	30.0	41.0
32-33	37.419980337219755	40.0	38.0	41.0	30.0	41.0
34-35	37.352259453938885	40.0	38.0	41.0	30.0	41.0
36-37	37.25811643550909	40.0	38.0	41.0	30.0	41.0
38-39	37.15739513787088	40.0	38.0	41.0	30.0	41.0
40-41	37.07660028248247	40.0	38.0	41.0	30.0	41.0
42-43	36.95958546279152	40.0	38.0	41.0	30.0	41.0
44-45	36.84215584705586	40.0	37.0	41.0	30.0	41.0
46-47	36.72629153252332	40.0	37.0	41.0	29.0	41.0
48-49	36.609116585327016	40.0	37.0	41.0	29.0	41.0
50-51	36.492285254208994	40.0	37.0	41.0	29.0	41.0
52-53	36.34450281177318	40.0	36.0	41.0	29.0	41.0
54-55	36.19191511838358	40.0	36.0	41.0	29.0	41.0
56-57	36.01646308172623	40.0	35.0	41.0	28.5	41.0
58-59	35.809100375159005	39.0	35.0	41.0	28.0	41.0
60-61	35.64529750484057	39.0	35.0	41.0	28.0	41.0
62-63	35.48571498137586	39.0	35.0	41.0	28.0	41.0
64-65	35.23503613946184	39.0	35.0	41.0	28.0	41.0
66-67	34.943901192993785	38.0	35.0	40.0	27.0	41.0
68-69	34.63430957735771	37.5	34.0	40.0	26.5	41.0
70-71	34.383663199650265	37.0	34.0	40.0	26.0	41.0
72-73	34.136877868996834	37.0	34.0	39.5	26.0	41.0
74-75	33.790435211204894	36.0	34.0	39.0	26.0	41.0
76-77	33.43569323255098	36.0	34.0	39.0	26.0	40.5
78-79	33.083499255407105	35.0	34.0	37.5	26.0	39.5
80-81	32.72451806556322	35.0	33.5	37.0	25.0	39.0
82-83	32.37914870076271	35.0	33.0	37.0	25.0	39.0
84-85	32.06949884697228	35.0	33.0	36.0	25.0	38.5
86-87	31.78902608915768	35.0	33.0	36.0	24.0	37.0
88-89	31.52317045002629	35.0	33.0	36.0	24.0	37.0
90-91	31.299458505808573	35.0	33.0	35.0	23.0	36.5
92-93	31.088462220986045	35.0	32.0	35.0	21.5	36.0
94-95	30.87275884556404	35.0	32.0	35.0	20.0	36.0
96-97	30.68340346406245	35.0	32.0	35.0	18.5	36.0
98-99	30.563429904842586	35.0	32.0	35.0	16.5	36.0
100	30.514775710719352	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.37215963247517436
1101	2	0.3843169841216749
1101	3	0.420186107372281
1101	4	0.4699281573006857
1101	5	0.3477452317167149
1101	6	0.3654508039254196
1101	7	0.332564319011432
1101	8	0.4438957453288168
1101	9	0.5109683335297888
1101	10-11	0.45618008899302964
1101	12-13	0.4377186675373608
1101	14-15	0.5161028849657257
1101	16-17	0.47567589281545963
1101	18-19	0.46404029025904237
1101	20-21	0.3489143384827855
1101	22-23	0.39578300447715975
1101	24-25	0.3853788797186226
1101	26-27	0.3499485304704777
1101	28-29	0.34814590735050643
1101	30-31	0.4167522172389724
1101	32-33	0.42651873304595256
1101	34-35	0.4258238881488978
1101	36-37	0.4056489512581365
1101	38-39	0.39038557238171023
1101	40-41	0.46800151413532376
1101	42-43	0.4462671963778675
1101	44-45	0.377152157184625
1101	46-47	0.4172451233498151
1101	48-49	0.4455653220131808
1101	50-51	0.5012907065380432
1101	52-53	0.5097023158320297
1101	54-55	0.5195876941699922
1101	56-57	0.44334184783264163
1101	58-59	0.48157308273252397
1101	60-61	0.48476724342282296
1101	62-63	0.5303377024267419
1101	64-65	0.49314535982784236
1101	66-67	0.418165377518001
1101	68-69	0.3864240089101685
1101	70-71	0.46223974647422494
1101	72-73	0.43126133026593294
1101	74-75	0.4544925158760833
1101	76-77	0.4900954858799338
1101	78-79	0.4026764572124719
1101	80-81	0.4276556911396341
1101	82-83	0.4637849159599554
1101	84-85	0.44582443264548743
1101	86-87	0.41127543952934786
1101	88-89	0.3838200061104473
1101	90-91	0.3887146008601867
1101	92-93	0.38157611042709405
1101	94-95	0.45818262074664773
1101	96-97	0.376319443552358
1101	98-99	0.3700326530573115
1101	100	0.4945859472015286
1102	1	0.20214584727568408
1102	2	0.24435192817077578
1102	3	0.2662141365739785
1102	4	0.2828065854353241
1102	5	0.20739531091199837
1102	6	0.21404077628184126
1102	7	0.24992995751703262
1102	8	0.24252796122560483
1102	9	0.37624493405608206
1102	10-11	0.33534874407490634
1102	12-13	0.30140174880177284
1102	14-15	0.3142521719626359
1102	16-17	0.36387079880879014
1102	18-19	0.3054980094182511
1102	20-21	0.29557479053850955
1102	22-23	0.2720058951768678
1102	24-25	0.341311252336169
1102	26-27	0.3718435909647084
1102	28-29	0.35676574559283836
1102	30-31	0.36639241906305386
1102	32-33	0.3632101589504728
1102	34-35	0.39125575326934836
1102	36-37	0.4231490554979942
1102	38-39	0.4009215577735006
1102	40-41	0.3981751126666495
1102	42-43	0.3828431358147242
1102	44-45	0.3354357340954408
1102	46-47	0.34608867530790377
1102	48-49	0.22892109454152632
1102	50-51	0.23156662219987112
1102	52-53	0.25670735367441466
1102	54-55	0.23017155868459582
1102	56-57	0.2211446808288926
1102	58-59	0.2854398707250567
1102	60-61	0.3168151728361579
1102	62-63	0.30105005183697386
1102	64-65	0.3175457547262326
1102	66-67	0.3234284492085564
1102	68-69	0.311943537173363
1102	70-71	0.3276655732952065
1102	72-73	0.3526591482175121
1102	74-75	0.36330373309657915
1102	76-77	0.31498758841777175
1102	78-79	0.30849530292436356
1102	80-81	0.2992693629497367
1102	82-83	0.29891852935841
1102	84-85	0.2694181043504926
1102	86-87	0.25753797771809417
1102	88-89	0.25032995201720354
1102	90-91	0.2789019492304803
1102	92-93	0.28442257314510044
1102	94-95	0.309967636855081
1102	96-97	0.30093659105576265
1102	98-99	0.23196711990149765
1102	100	0.276486794051543
1103	1	0.09015211876801033
1103	2	0.05676840370636427
1103	3	0.09995576154098629
1103	4	0.12618800493061855
1103	5	0.03970272635832117
1103	6	0.06661448353129629
1103	7	0.03278534716926629
1103	8	0.14556855084941134
1103	9	0.17111402605465287
1103	10-11	0.11171518783060463
1103	12-13	0.09207012032592132
1103	14-15	0.09218529232308015
1103	16-17	0.06793042047303288
1103	18-19	0.040918376922228106
1103	20-21	0.032412165155676576
1103	22-23	0.044779038031073526
1103	24-25	0.0466331326997107
1103	26-27	0.06180422199150826
1103	28-29	0.06217025611536542
1103	30-31	0.019353186585888693
1103	32-33	-0.007577259582262741
1103	34-35	0.06581744593741234
1103	36-37	0.06487920258896196
1103	38-39	0.04107231874500883
1103	40-41	-0.008285264291941985
1103	42-43	-0.02487537321879074
1103	44-45	-0.042638338567826395
1103	46-47	-0.04586542051329445
1103	48-49	-0.045766667721942156
1103	50-51	-0.13636218107662046
1103	52-53	-0.15504018140006792
1103	54-55	-0.14793120500608126
1103	56-57	-0.24891253274247305
1103	58-59	-0.19967736081299847
1103	60-61	-0.16306650780853715
1103	62-63	-0.13684636354268065
1103	64-65	-0.08333064017657676
1103	66-67	-0.09741885421334473
1103	68-69	-0.13786147372068314
1103	70-71	-0.08747342435506056
1103	72-73	-0.11706007284184494
1103	74-75	-0.07797398701426772
1103	76-77	-0.11849802712197288
1103	78-79	-0.14654755414523635
1103	80-81	-0.1204846902378165
1103	82-83	-0.1707481321804991
1103	84-85	-0.11761921620544769
1103	86-87	-0.1320369970643256
1103	88-89	-0.15843932011218342
1103	90-91	-0.20034154529270864
1103	92-93	-0.13865302197395835
1103	94-95	-0.09505839628439716
1103	96-97	-0.11671366507575343
1103	98-99	-0.1371116197541653
1103	100	-0.22262832582689285
1104	1	0.30029594630147116
1104	2	0.27137452998364253
1104	3	0.28652259282451453
1104	4	0.2272251410965609
1104	5	0.2740304187761211
1104	6	0.30046879844052654
1104	7	0.2786518660303017
1104	8	0.23626561754004882
1104	9	0.32536967986988685
1104	10-11	0.29518808763402404
1104	12-13	0.33745826824473824
1104	14-15	0.3769692181273143
1104	16-17	0.31025884193213926
1104	18-19	0.3110112880773883
1104	20-21	0.27868007285704266
1104	22-23	0.3026494384545231
1104	24-25	0.3456019886541313
1104	26-27	0.3466522301940529
1104	28-29	0.4317932019416091
1104	30-31	0.43149721718565814
1104	32-33	0.4029511455649626
1104	34-35	0.4323993074561798
1104	36-37	0.4090327240415874
1104	38-39	0.3667993210702818
1104	40-41	0.32269483021711665
1104	42-43	0.30569401137380225
1104	44-45	0.36493919697294075
1104	46-47	0.3618357857338239
1104	48-49	0.2841917702654442
1104	50-51	0.2624219178333149
1104	52-53	0.35365596649877773
1104	54-55	0.2991260813290495
1104	56-57	0.31595499978558905
1104	58-59	0.3286341595377422
1104	60-61	0.3032750031046163
1104	62-63	0.29473702097334353
1104	64-65	0.3023782359846052
1104	66-67	0.34797445358690027
1104	68-69	0.30612584961580325
1104	70-71	0.2873749833221737
1104	72-73	0.233514005578364
1104	74-75	0.29622244467805814
1104	76-77	0.29053983808528017
1104	78-79	0.2438992582964019
1104	80-81	0.29459306584627143
1104	82-83	0.3425319877950628
1104	84-85	0.31303072911644847
1104	86-87	0.3126889383306306
1104	88-89	0.2979962171563102
1104	90-91	0.2750198544666098
1104	92-93	0.24989262383513733
1104	94-95	0.2672063089615371
1104	96-97	0.30789971832507135
1104	98-99	0.2938868672354431
1104	100	0.29566045775602845
1105	1	0.06603054859223789
1105	2	0.1442314269088527
1105	3	0.13565377209893015
1105	4	0.12054097588362822
1105	5	0.17729742476468147
1105	6	0.1424116424156736
1105	7	0.1628665108003915
1105	8	0.1729007980211179
1105	9	0.1734859928718393
1105	10-11	0.14594987743519283
1105	12-13	0.1687151291320177
1105	14-15	0.16323727180515846
1105	16-17	0.2137878905601056
1105	18-19	0.17901120982194385
1105	20-21	0.14578349811132796
1105	22-23	0.14236055843287687
1105	24-25	0.12216659581493161
1105	26-27	0.1280184898826775
1105	28-29	0.1540260790752157
1105	30-31	0.13362705990905255
1105	32-33	0.2004754460221676
1105	34-35	0.12799577108012983
1105	36-37	0.15195436026186115
1105	38-39	0.18405165786881383
1105	40-41	0.14636334342671375
1105	42-43	0.15757285433095802
1105	44-45	0.107074055552701
1105	46-47	0.07238162612246413
1105	48-49	0.12565018608744083
1105	50-51	0.13554339612775124
1105	52-53	0.1495568996299852
1105	54-55	0.10922947525227755
1105	56-57	0.10004368024141286
1105	58-59	0.03389117597335911
1105	60-61	0.03997610152518405
1105	62-63	0.09692079305614243
1105	64-65	0.09288531875206019
1105	66-67	0.12495897388029675
1105	68-69	0.08182059890167181
1105	70-71	0.06302148470943081
1105	72-73	0.0018620196683869494
1105	74-75	0.03633762937703011
1105	76-77	0.03731050155332838
1105	78-79	0.038055013197464405
1105	80-81	-0.005344231136561461
1105	82-83	0.030880678558858676
1105	84-85	0.05271451022316853
1105	86-87	-0.0035933028764247865
1105	88-89	0.029712982788517195
1105	90-91	0.06602971402696411
1105	92-93	0.0422680914366822
1105	94-95	0.09902613018009276
1105	96-97	0.15001145400917437
1105	98-99	0.12197855768636146
1105	100	0.1450586247786525
1106	1	0.19785423006753788
1106	2	0.21390216547106888
1106	3	0.20900677880044327
1106	4	0.1949601121400164
1106	5	0.14688880049736497
1106	6	0.1415798178645602
1106	7	0.2483502733823144
1106	8	0.18461595785753815
1106	9	0.19830039035300473
1106	10-11	0.24196217477733484
1106	12-13	0.22431139448237758
1106	14-15	0.25629705052664065
1106	16-17	0.2711698816918471
1106	18-19	0.29227869395474926
1106	20-21	0.2703226498946947
1106	22-23	0.2584351624290733
1106	24-25	0.28449239316177
1106	26-27	0.33092998875141433
1106	28-29	0.3494962651582938
1106	30-31	0.39031136514434905
1106	32-33	0.3293425543363355
1106	34-35	0.35855905517207276
1106	36-37	0.3481375130024986
1106	38-39	0.3089862798156773
1106	40-41	0.32900093154661647
1106	42-43	0.31256349105305503
1106	44-45	0.3110192589466294
1106	46-47	0.3283220078885023
1106	48-49	0.3090179754853537
1106	50-51	0.2158154365927416
1106	52-53	0.22491165215562603
1106	54-55	0.21874561723618768
1106	56-57	0.2540371597723663
1106	58-59	0.2308658676221711
1106	60-61	0.22296968733252243
1106	62-63	0.32860023905457325
1106	64-65	0.26329143123721366
1106	66-67	0.31408133076321576
1106	68-69	0.3353543116149993
1106	70-71	0.3342139747518118
1106	72-73	0.3125133539227747
1106	74-75	0.2946226988084959
1106	76-77	0.28631521351662315
1106	78-79	0.32542623777371205
1106	80-81	0.3190451624114843
1106	82-83	0.3559567350744146
1106	84-85	0.3032098135632211
1106	86-87	0.3204029357749505
1106	88-89	0.35289698065802355
1106	90-91	0.36787713884601203
1106	92-93	0.41210063891574933
1106	94-95	0.44259825486432547
1106	96-97	0.46323454148865295
1106	98-99	0.46335257933083085
1106	100	0.4736894968176948
1107	1	0.13757221265275987
1107	2	0.1893308963927005
1107	3	0.18494506285590617
1107	4	0.1604142852722248
1107	5	0.17977807837083049
1107	6	0.20714687169060397
1107	7	0.21815870838355522
1107	8	0.1592489538180999
1107	9	0.18092381221588738
1107	10-11	0.20094131443321572
1107	12-13	0.22040185986154626
1107	14-15	0.3304726599112371
1107	16-17	0.2970188212756568
1107	18-19	0.3034756668157783
1107	20-21	0.2683189011132754
1107	22-23	0.320858965935237
1107	24-25	0.29851045164218704
1107	26-27	0.3266384968931888
1107	28-29	0.28665769143714925
1107	30-31	0.3348345145987466
1107	32-33	0.3431139686019975
1107	34-35	0.3651140955998855
1107	36-37	0.3854739114460344
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2216	38-39	-0.4439923873932017
2216	40-41	-0.5286654944090827
2216	42-43	-0.5602978059772923
2216	44-45	-0.49709602833090116
2216	46-47	-0.39519821357956175
2216	48-49	-0.5115587770756562
2216	50-51	-0.4514716023269756
2216	52-53	-0.5635228321883687
2216	54-55	-0.6413047207024434
2216	56-57	-0.4725919975575863
2216	58-59	-0.4137714920475304
2216	60-61	-0.39869929560948947
2216	62-63	-0.4832297791751756
2216	64-65	-0.502444733821342
2216	66-67	-0.5331297060267843
2216	68-69	-0.5778187445478622
2216	70-71	-0.5910988796330727
2216	72-73	-0.5446891531745237
2216	74-75	-0.5808611249952662
2216	76-77	-0.4757227541840834
2216	78-79	-0.4493037746566131
2216	80-81	-0.39652544835154657
2216	82-83	-0.35399392965818777
2216	84-85	-0.3264011028363569
2216	86-87	-0.31814858510610833
2216	88-89	-0.3590771671937105
2216	90-91	-0.3948650743812827
2216	92-93	-0.46436713027003407
2216	94-95	-0.46558908849013037
2216	96-97	-0.6249698787193303
2216	98-99	-0.6962830824356772
2216	100	-0.6815121134653097
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	78583.0
3	17892.0
4	12483.0
5	11176.0
6	7319.0
7	7896.0
8	8809.0
9	8740.0
10	8944.0
11	9716.0
12	11521.0
13	12704.0
14	13762.0
15	13681.0
16	12878.0
17	12442.0
18	11662.0
19	10976.0
20	10783.0
21	11168.0
22	14100.0
23	24471.0
24	42479.0
25	46869.0
26	46912.0
27	34586.0
28	36011.0
29	41995.0
30	48908.0
31	59190.0
32	77315.0
33	103292.0
34	145397.0
35	235238.0
36	400339.0
37	807207.0
38	1364512.0
39	733306.0
40	3605.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.129994759646785	22.67515535157637	13.979744466426094	22.215105422350753
2	14.618472767984642	23.318911605571742	40.43616586770997	21.62644975873365
3	16.32285309548471	30.389618821873686	31.013853006427478	22.27367507621413
4	11.269476621692734	20.318399565856662	41.27320781519427	27.138915997256337
5	11.301516649096508	40.9409708466974	35.78087182207154	11.976640682134548
6	25.956186043593725	42.549848460154685	19.365315987503035	12.128649508748556
7	23.264091714015787	35.77956979223127	25.054821735312743	15.9015167584402
8	22.52524585604274	38.45843715116059	22.029859612048345	16.98645738074833
9	24.272346615946464	19.12966533132026	22.245176268577257	34.35281178415602
10-11	21.37986258427807	29.879068637010032	30.34072939614163	18.400339382570273
12-13	22.48079402184797	28.32315573145696	29.43399094555731	19.76205930113776
14-15	20.508110896852223	29.55808976221504	27.08193724449518	22.851862096437557
16-17	19.803363423412	32.33317620364885	27.432177775749985	20.43128259718917
18-19	19.75645483844999	31.38670200293187	29.344571798212144	19.512271360405993
20-21	21.535800452173753	30.468107536368137	28.48879995841072	19.50729205304739
22-23	20.85307599453987	30.475401015208387	28.629339263461734	20.04218372679001
24-25	20.528225543759007	30.655160591436427	28.585797743167323	20.23081612163724
26-27	20.34584470220342	30.588762514896793	28.606789801062416	20.458602981837373
28-29	20.429374228289618	30.37918193270389	28.95761161709697	20.233832221909523
30-31	20.84726314674238	30.800832750768997	28.31376743388215	20.038136668606477
32-33	20.16977025212624	30.585581900064206	28.87276597452832	20.371881873281232
34-35	20.5125747252552	30.15022153530691	28.92842673409863	20.408777005339264
36-37	20.911917368942763	30.48417512509139	28.261364062605903	20.34254344335994
38-39	20.593702338760924	30.569251088044464	28.62727734763923	20.209769225555384
40-41	20.406495736769685	30.29020149085288	28.76065478549824	20.542647986879196
42-43	20.224345215598525	30.353989269702318	28.9149146048788	20.506750909820358
44-45	20.47717119187728	30.3283798364813	28.718462284598345	20.475986687043076
46-47	20.517082424207594	30.58843348577618	28.542157514136733	20.35232657587949
48-49	20.398006785457877	30.219350553547624	28.808331105075013	20.574311555919486
50-51	20.528379090681963	30.196515932577107	28.552094193579237	20.723010783161694
52-53	20.45044305964618	30.33634234120013	28.57303141328756	20.64018318586614
54-55	20.599537121833123	30.56147503316065	28.454548026954946	20.384439818051284
56-57	20.550149850829165	30.366185282439695	28.371709462022032	20.71195540470911
58-59	20.41115698264503	29.91926502791154	28.75460064967897	20.914977339764462
60-61	20.799861018099453	30.404188145870453	28.32201509717217	20.473935738857925
62-63	20.488259473241925	30.370133631887047	28.474158162543457	20.66744873232757
64-65	20.529289404582325	30.143640952894657	28.53185890266156	20.79521073986146
66-67	20.469976421773218	30.18440766093856	28.379430678719075	20.966185238569143
68-69	20.867904240242147	30.353660240581704	28.323353148929325	20.455082370246817
70-71	20.85041085866291	30.110277400064533	28.39043121898489	20.648880522287666
72-73	20.54910792528056	30.257057290769833	28.50385852449742	20.689976259452184
74-75	20.59903261051485	30.271984245208294	28.43319403702718	20.695789107249674
76-77	20.733342297548933	30.39780498093057	28.1818925623406	20.6869601591799
78-79	20.832051033732725	30.288007963382128	28.387371248163195	20.492569754721952
80-81	20.7347022845808	30.355020227613572	28.33474852413988	20.575528963665754
82-83	20.552780132774277	30.34582072511469	28.484765396650264	20.616633745460767
84-85	20.59599106659397	30.179221989085608	28.35282676406856	20.871960180251854
86-87	20.843287932355338	30.34336169043651	28.188441203841563	20.624909173366586
88-89	20.551932589953292	30.23489890927884	28.390985027179738	20.822183473588133
90-91	20.561074856046066	30.256956402522622	28.282292295037013	20.899676446394295
92-93	20.663837620609282	30.406750226294072	28.26672615626043	20.662685996836217
94-95	20.610604219835594	30.045015337986847	28.382311537550788	20.962068904626776
96-97	20.4851838345174	30.351612133708798	28.364439009276044	20.798765022497758
98-99	20.334378391480573	30.276813364167758	28.323177971190617	21.065630273161055
100	20.374050724542307	30.336715846922612	28.321509890890905	20.967723537644172
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	32115.0
1	22939.0
2	11214.0
3	6579.0
4	3372.0
5	1901.0
6	1549.0
7	1918.0
8	2717.5
9	2998.0
10	2072.0
11	1180.5
12	1068.5
13	1119.0
14	1220.0
15	1384.0
16	1635.0
17	2078.5
18	2728.0
19	3788.0
20	5436.0
21	7607.5
22	10934.0
23	15852.5
24	22571.0
25	30868.0
26	41893.5
27	55840.5
28	72204.5
29	89495.0
30	105473.0
31	119544.0
32	132455.5
33	144069.0
34	152933.0
35	160728.5
36	166589.0
37	171350.5
38	175268.0
39	174466.5
40	173721.0
41	172296.0
42	168188.0
43	166960.0
44	163831.5
45	155492.5
46	147778.0
47	141230.0
48	134146.5
49	128301.0
50	124248.0
51	115924.0
52	103282.5
53	92944.0
54	85369.5
55	80007.0
56	72331.5
57	63415.0
58	57950.0
59	53045.5
60	46950.0
61	39705.5
62	31844.5
63	23555.5
64	17004.0
65	13146.0
66	9995.0
67	7496.0
68	5525.0
69	4025.0
70	3031.0
71	2586.0
72	2092.5
73	1389.5
74	1030.0
75	827.5
76	595.0
77	451.5
78	339.5
79	248.0
80	183.5
81	146.5
82	138.0
83	125.0
84	108.0
85	91.5
86	79.0
87	70.0
88	67.0
89	60.0
90	51.0
91	45.0
92	36.0
93	28.0
94	23.0
95	22.5
96	19.0
97	22.5
98	27.5
99	26.5
100	57.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	3.070938459051339E-4
2	1.0967637353754782E-4
3	2.1935274707509564E-5
4	8.774109883003825E-5
5	2.1935274707509564E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	4.387054941501913E-5
84-85	5.154789556264747E-4
86-87	9.761197244841756E-4
88-89	0.0030709384590513387
90-91	0.002566427140778619
92-93	0.0018974012621995773
94-95	0.001919336536907087
96-97	0.0017438543392470105
98-99	0.002401912580472297
100	0.0027857798878537145
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4558867.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.428245418945934
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.24152526027703	48.22052895482287
2	14.474161978122135	18.071930724075695
3	4.195249348495338	7.8570616776462
4	1.669551252176564	4.169086212415482
5	0.7716119508350255	2.408519011746031
6	0.43458318452115074	1.6278159418940077
7	0.24852576081288974	1.0860519032270137
8	0.1653796622189596	0.8259489712246074
9	0.12246227814006336	0.6880594638861983
>10	0.5867063201295547	6.741710578347495
>50	0.054148845891793924	2.3751268278135655
>100	0.03373676590627919	3.9237341736037608
>500	0.0017944334990523875	0.7175203291175185
>1k	5.277740383274197E-4	0.5170201288319728
>5k	0.0	0.0
>10k+	3.5184935888372E-5	0.7698851013475527
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	35050	0.7688313784982103	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	2.1935274707509564E-5	0.0	0.0	0.0	0.0
6	2.1935274707509564E-5	0.0	0.0	0.0	0.0
7	2.1935274707509564E-5	0.0	0.0	0.0	0.0
8	2.1935274707509564E-5	0.0	0.0	4.387054941501913E-5	0.0
9	2.1935274707509564E-5	0.0	0.0	4.387054941501913E-5	0.0
10-11	6.58058241225287E-5	0.0	0.0	4.387054941501913E-5	0.0
12-13	6.58058241225287E-5	0.0	0.0	5.4838186768773914E-5	0.0
14-15	6.58058241225287E-5	0.0	0.0	1.0967637353754782E-4	0.0
16-17	1.316116482450574E-4	0.0	0.0	1.0967637353754782E-4	0.0
18-19	1.316116482450574E-4	0.0	0.0	1.2064401089130261E-4	0.0
20-21	1.4257928559881217E-4	0.0	0.0	2.303203844288504E-4	0.0
22-23	1.9741747236758607E-4	0.0	0.0	5.703171423952487E-4	0.0
24-25	2.412880217826052E-4	0.0	0.0	0.0011845048342055164	0.0
26-27	2.412880217826052E-4	0.0	0.0	0.0020728834598596536	0.0
28-29	2.412880217826052E-4	0.0	0.0	0.004178669831780572	0.0
30-31	2.7419093384386953E-4	0.0	0.0	0.008828948069772599	0.0
32-33	3.070938459051339E-4	0.0	0.0	0.01708757899714995	0.0
34-35	3.2902912061264346E-4	0.0	0.0	0.028614565855946225	0.0
36-37	3.2902912061264346E-4	0.0	0.0	0.04557053320485112	0.0
38-39	3.2902912061264346E-4	0.0	0.0	0.07020384670138435	0.0
40-41	3.2902912061264346E-4	0.0	0.0	0.09891712129351438	0.0
42-43	3.2902912061264346E-4	0.0	0.0	0.12849683923659103	0.0
44-45	3.2902912061264346E-4	0.0	0.0	0.16152039530874668	0.0
46-47	3.2902912061264346E-4	0.0	0.0	0.19979744967335086	0.0
48-49	3.6193203267390777E-4	0.0	0.0	0.2421105945841368	0.0
50-51	3.9483494473517214E-4	0.0	0.0	0.28803209218430803	0.0
52-53	4.167702194426817E-4	0.0	0.0	0.33351488429032916	0.0
54-55	4.3870549415019126E-4	0.0	0.0	0.3827705436460419	0.0
56-57	4.49673131503946E-4	0.0	0.0	0.4424344908504679	0.0
58-59	4.825760435652104E-4	0.0	0.0	0.5128028521121586	0.0
60-61	5.483818676877391E-4	0.0	0.0	0.5893788961160744	0.0
62-63	5.483818676877391E-4	0.0	0.0	0.6663936456141406	0.0
64-65	5.483818676877391E-4	1.0967637353754782E-5	0.0	0.748979954887914	0.0
66-67	5.483818676877391E-4	2.1935274707509564E-5	0.0	0.8409545617365017	0.0
68-69	5.483818676877391E-4	2.1935274707509564E-5	0.0	0.9456735631901523	0.0
70-71	5.483818676877391E-4	2.1935274707509564E-5	0.0	1.0671511145203403	0.0
72-73	5.483818676877391E-4	2.1935274707509564E-5	0.0	1.1990917918860102	0.0
74-75	5.703171423952487E-4	2.1935274707509564E-5	0.0	1.334706627765188	0.0
76-77	6.141876918102678E-4	2.1935274707509564E-5	1.0967637353754782E-5	1.4820678032502375	0.0
78-79	6.141876918102678E-4	2.1935274707509564E-5	2.1935274707509564E-5	1.6462643020733003	0.0
80-81	6.141876918102678E-4	2.1935274707509564E-5	2.1935274707509564E-5	1.8356753991726453	0.0
82-83	6.141876918102678E-4	2.1935274707509564E-5	2.1935274707509564E-5	2.041746337412344	0.0
84-85	6.361229665177773E-4	2.1935274707509564E-5	2.1935274707509564E-5	2.2579184696548507	0.0
86-87	6.580582412252869E-4	2.1935274707509564E-5	2.1935274707509564E-5	2.482162782989721	0.0
88	6.580582412252869E-4	2.1935274707509564E-5	2.1935274707509564E-5	2.662635255645756	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5240	0.0	40.0932	1
GTATCAA	9370	0.0	35.7137	1
TCAACGC	9875	0.0	31.983547	4
ATCAACG	9960	0.0	31.804974	3
CAACGCA	10455	0.0	30.254185	5
AACGCAG	11000	0.0	28.926138	6
ACGCAGA	12155	0.0	26.022831	7
CGCAGAG	12025	0.0	25.952394	8
GTACATG	17625	0.0	25.866465	1
TACATGG	16850	0.0	25.66152	2
TATCAAC	12980	0.0	25.129234	2
ACATGGG	17335	0.0	23.425257	3
GCAGAGT	13745	0.0	21.337048	9
CATGGGG	8650	0.0	20.212559	4
CATGGGT	4275	0.0	19.239616	4
AGAGTAC	13055	0.0	17.136597	10-11
CATGGGA	5755	0.0	16.49683	4
CAGAGTA	14165	0.0	16.274845	10-11
CTGTCGC	1005	0.0	15.9003725	9
TCTGTCG	940	0.0	15.499878	8
>>END_MODULE