FastQCFastQC Report
Wed 25 May 2016
SRR2431370_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431370_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4558867
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT299140.656171807600441No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205010.44969506677865356No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164060.3598701168514019No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87380.19167043039421855No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA75130.16479971887751935No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93350.060.7252121
GTATCAA203150.048.3345451
ATCAACG264650.036.8691373
TCAACGC267500.036.5286334
CAACGCA273400.035.7047845
TATCAAC284200.034.5479162
AACGCAG283700.034.4581876
ACGCAGA318650.030.5017687
CGCAGAG318650.030.4427728
GCAGAGT341600.028.0673079
GTACATG163550.024.745071
TACATGG159650.024.4646822
ACATGGG160050.023.4050793
AGAGTAC332250.023.2130410-11
GAGTACT257700.022.11341312-13
AGTACTT269800.021.96653612-13
CAGAGTA343400.021.59024210-11
CATGGGT41050.020.3802534
ATAACGC4900.020.1433053
GTGGTAT36050.019.8186651
Produced by FastQC (version 0.11.5)