##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431370_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4558867
Sequences flagged as poor quality	0
Sequence length	100
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.74382253309869	34.0	31.0	34.0	31.0	34.0
2	32.87459033132574	34.0	31.0	34.0	31.0	34.0
3	32.950334809065495	34.0	31.0	34.0	31.0	34.0
4	36.338678667309225	37.0	37.0	37.0	35.0	37.0
5	36.295488330762886	37.0	37.0	37.0	35.0	37.0
6	36.32858383453608	37.0	37.0	37.0	35.0	37.0
7	36.19212449058067	37.0	37.0	37.0	35.0	37.0
8	36.24954774947372	37.0	37.0	37.0	35.0	37.0
9	38.06490647785952	39.0	39.0	39.0	35.0	39.0
10-11	38.060418191625246	39.0	39.0	39.0	35.0	39.0
12-13	38.039192523054524	39.0	39.0	39.0	35.0	39.0
14-15	39.51996219674757	41.0	39.0	41.0	36.5	41.0
16-17	39.537316837714286	41.0	39.5	41.0	37.0	41.0
18-19	39.61214156938555	41.0	40.0	41.0	37.0	41.0
20-21	39.61421006579047	41.0	40.0	41.0	37.0	41.0
22-23	39.53244183258691	41.0	40.0	41.0	36.5	41.0
24-25	39.50297782321792	41.0	40.0	41.0	36.0	41.0
26-27	39.41032179706055	41.0	39.0	41.0	36.0	41.0
28-29	39.31900437104219	41.0	39.0	41.0	36.0	41.0
30-31	39.137345090348106	41.0	39.0	41.0	35.0	41.0
32-33	38.914419635405025	41.0	39.0	41.0	35.0	41.0
34-35	38.80010066097563	41.0	38.5	41.0	35.0	41.0
36-37	38.679155478762596	41.0	38.0	41.0	35.0	41.0
38-39	38.555451387373225	41.0	38.0	41.0	35.0	41.0
40-41	38.45762839758212	41.0	38.0	41.0	35.0	41.0
42-43	38.323453173782	41.0	38.0	41.0	35.0	41.0
44-45	38.11670366781922	41.0	38.0	41.0	34.0	41.0
46-47	37.80526905040222	40.5	38.0	41.0	33.0	41.0
48-49	37.54000697980442	40.0	38.0	41.0	33.0	41.0
50-51	37.40257041936078	40.0	38.0	41.0	33.0	41.0
52-53	37.3058348708133	40.0	38.0	41.0	33.0	41.0
54-55	37.173876974256984	40.0	37.0	41.0	32.5	41.0
56-57	37.031396507070724	40.0	37.0	41.0	32.0	41.0
58-59	36.865789241054856	40.0	36.5	41.0	32.0	41.0
60-61	36.68534671004879	40.0	36.0	41.0	31.0	41.0
62-63	36.471281570618316	39.5	35.5	41.0	31.0	41.0
64-65	36.216746068705234	39.0	35.0	41.0	31.0	41.0
66-67	35.94562475720393	39.0	35.0	41.0	31.0	41.0
68-69	35.68752872150032	38.5	35.0	41.0	30.5	41.0
70-71	35.435436699513275	37.5	35.0	40.0	30.0	41.0
72-73	35.172971705469806	37.0	35.0	40.0	30.0	41.0
74-75	34.86827319156273	36.5	35.0	39.0	30.0	41.0
76-77	34.53526972820221	36.0	35.0	39.0	30.0	41.0
78-79	34.19782228786231	36.0	35.0	38.5	30.0	40.0
80-81	33.86723499501082	35.0	35.0	37.0	29.5	39.0
82-83	33.52507629636925	35.0	35.0	37.0	29.0	39.0
84-85	33.234256669475116	35.0	34.0	36.5	29.0	39.0
86-87	32.98706904588355	35.0	34.0	36.0	29.0	37.0
88-89	32.74271798234079	35.0	34.0	36.0	29.0	37.0
90-91	32.528010797419626	35.0	34.0	35.5	29.0	37.0
92-93	32.365310394007984	35.0	34.0	35.0	29.0	36.0
94-95	32.2185221678983	35.0	34.0	35.0	29.0	36.0
96-97	32.075229766518746	35.0	34.0	35.0	28.5	36.0
98-99	31.949863749041157	35.0	34.0	35.0	27.0	36.0
100	31.8435005890718	35.0	34.0	35.0	27.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.15460345758184246
1101	2	0.11982977497569891
1101	3	0.15848690588583025
1101	4	0.10831083290420196
1101	5	0.13141075279499148
1101	6	0.13256918600968248
1101	7	0.1443034731652162
1101	8	0.16926273573471207
1101	9	0.22616296835617788
1101	10-11	0.18484245167247337
1101	12-13	0.18926879611525038
1101	14-15	0.24921612569619356
1101	16-17	0.23855675543001809
1101	18-19	0.20084388451709856
1101	20-21	0.23371484860848568
1101	22-23	0.2509490764131499
1101	24-25	0.19279900097585312
1101	26-27	0.16540671794840733
1101	28-29	0.20941246116380796
1101	30-31	0.26206009722960033
1101	32-33	0.25618962020118374
1101	34-35	0.23637826253806082
1101	36-37	0.2146533364576939
1101	38-39	0.22162701805730478
1101	40-41	0.26383568562174986
1101	42-43	0.30988712119629014
1101	44-45	0.3719792308186456
1101	46-47	0.333622093082667
1101	48-49	0.33321862772726263
1101	50-51	0.3192208118190152
1101	52-53	0.29939012045654323
1101	54-55	0.29821223128345054
1101	56-57	0.2618117691051651
1101	58-59	0.25042727191491565
1101	60-61	0.26129180234418925
1101	62-63	0.27948298979109865
1101	64-65	0.3236099809900068
1101	66-67	0.2625943649588791
1101	68-69	0.256107507390702
1101	70-71	0.2198237173136377
1101	72-73	0.2298374606634752
1101	74-75	0.26873812745911607
1101	76-77	0.24698330107052868
1101	78-79	0.2552382235595374
1101	80-81	0.2863597286923252
1101	82-83	0.2902321931049201
1101	84-85	0.24140744945390225
1101	86-87	0.25637920112608725
1101	88-89	0.2750772563391024
1101	90-91	0.2728127049784561
1101	92-93	0.33149098856094383
1101	94-95	0.36890299192643994
1101	96-97	0.3664318828299997
1101	98-99	0.4042423288225656
1101	100	0.43957121219698436
1102	1	0.1549736379985731
1102	2	0.15062272033638635
1102	3	0.14113012224314048
1102	4	0.09466096105906274
1102	5	0.11564806547817597
1102	6	0.10650013566107219
1102	7	0.15487280548940419
1102	8	0.1754281120617165
1102	9	0.20216204171417473
1102	10-11	0.20002669985851895
1102	12-13	0.17464053067583052
1102	14-15	0.26389888071072676
1102	16-17	0.25650020443497823
1102	18-19	0.23570012166791088
1102	20-21	0.22972878517063577
1102	22-23	0.23534698477961769
1102	24-25	0.23414613993919886
1102	26-27	0.24882099985511985
1102	28-29	0.22177869711481435
1102	30-31	0.22097043931167093
1102	32-33	0.2584521039740224
1102	34-35	0.27637482618333564
1102	36-37	0.19342635646147954
1102	38-39	0.22579528638352286
1102	40-41	0.2667508533339813
1102	42-43	0.2547365376961963
1102	44-45	0.21986601999673638
1102	46-47	0.21254657642498387
1102	48-49	0.274818363564556
1102	50-51	0.3180273084104286
1102	52-53	0.2936514277063864
1102	54-55	0.2869486050221184
1102	56-57	0.259452056187385
1102	58-59	0.279517448748436
1102	60-61	0.2506777782549321
1102	62-63	0.1938048489803137
1102	64-65	0.24579845028869585
1102	66-67	0.2706105328679058
1102	68-69	0.2846944721386748
1102	70-71	0.2796202062401747
1102	72-73	0.2649122700125801
1102	74-75	0.25613655989791795
1102	76-77	0.2853640609611716
1102	78-79	0.23008453177173038
1102	80-81	0.21992088934826626
1102	82-83	0.26030124630201357
1102	84-85	0.2479371987237684
1102	86-87	0.24427530829163402
1102	88-89	0.2661901269633731
1102	90-91	0.25587779195328864
1102	92-93	0.23773592850773184
1102	94-95	0.21309935860381302
1102	96-97	0.23716715273118183
1102	98-99	0.2788304831572823
1102	100	0.3169974409491836
1103	1	0.05349625763832222
1103	2	0.09810326965113347
1103	3	0.11410124539552413
1103	4	0.1040580172863983
1103	5	0.07590212879915015
1103	6	0.08919258469542513
1103	7	0.04607953056856218
1103	8	0.0897571681122642
1103	9	0.15079291809442452
1103	10-11	0.18183961629046763
1103	12-13	0.13295533693081296
1103	14-15	0.23512547951517604
1103	16-17	0.2375468873004749
1103	18-19	0.21400452834112826
1103	20-21	0.20936806936491337
1103	22-23	0.22831768306062372
1103	24-25	0.1694762833186374
1103	26-27	0.13494697129058153
1103	28-29	0.1492501483216273
1103	30-31	0.19004959407156008
1103	32-33	0.12402279781096581
1103	34-35	0.056625375769122854
1103	36-37	0.09705389125271324
1103	38-39	0.08835906287479389
1103	40-41	0.10357979156817265
1103	42-43	0.026868967176703507
1103	44-45	0.06328897433258618
1103	46-47	0.06391313616569505
1103	48-49	0.03216128873566504
1103	50-51	0.01628107480979679
1103	52-53	0.048867371029515994
1103	54-55	3.936111595521652E-4
1103	56-57	0.031109704104281377
1103	58-59	-0.0026619192529295788
1103	60-61	-0.055505565468209284
1103	62-63	-0.004175009674248997
1103	64-65	-0.03528131882544727
1103	66-67	0.009858649698742283
1103	68-69	-0.03723668062774266
1103	70-71	-0.02759426859071823
1103	72-73	-0.025658808406220146
1103	74-75	-0.06449640323570094
1103	76-77	-0.10343582876761559
1103	78-79	-0.11276163524130567
1103	80-81	-0.056998009297196006
1103	82-83	-0.07246335761407607
1103	84-85	-0.07610047369404072
1103	86-87	-0.050678550431797476
1103	88-89	-0.03041642040234649
1103	90-91	-0.007391173365235204
1103	92-93	-0.028052585167628763
1103	94-95	-0.027178216708911407
1103	96-97	-0.07924913283317636
1103	98-99	-0.08264479238035705
1103	100	-0.023682626746992952
1104	1	0.09728567455508141
1104	2	0.12289616699113282
1104	3	0.13374175146338274
1104	4	0.032486134312506465
1104	5	0.07429857081123714
1104	6	0.12143884752646983
1104	7	0.09703768725917428
1104	8	0.07493449326302937
1104	9	0.151414267686377
1104	10-11	0.16877088500544346
1104	12-13	0.1704535268111158
1104	14-15	0.23595180955783235
1104	16-17	0.2772737298358763
1104	18-19	0.2703598377758851
1104	20-21	0.24128160306808866
1104	22-23	0.23561142512313893
1104	24-25	0.21055776117525227
1104	26-27	0.20029329088031744
1104	28-29	0.20342241179515952
1104	30-31	0.24689242907685838
1104	32-33	0.24937795577823607
1104	34-35	0.24263102833599248
1104	36-37	0.26764215102443956
1104	38-39	0.21653975526430003
1104	40-41	0.20014697239206214
1104	42-43	0.2152135440580949
1104	44-45	0.15763540877129145
1104	46-47	0.20344298774419656
1104	48-49	0.21151577092492602
1104	50-51	0.22969684973244142
1104	52-53	0.22382471878388088
1104	54-55	0.18266933524904516
1104	56-57	0.23824590333562412
1104	58-59	0.1960471949285818
1104	60-61	0.2576359035000877
1104	62-63	0.23108379166864523
1104	64-65	0.2816465044203156
1104	66-67	0.23719570147565605
1104	68-69	0.22613282180988392
1104	70-71	0.239816003568059
1104	72-73	0.16502394377465635
1104	74-75	0.17117394026260513
1104	76-77	0.1579890641648518
1104	78-79	0.12445209536961244
1104	80-81	0.13825398433117897
1104	82-83	0.18209598019004858
1104	84-85	0.16312228171592835
1104	86-87	0.17194861151690333
1104	88-89	0.14996371305549872
1104	90-91	0.14848726315739924
1104	92-93	0.16668708216981543
1104	94-95	0.18898486217568689
1104	96-97	0.18743900559811522
1104	98-99	0.21974023797139708
1104	100	0.2207218147457759
1105	1	-0.05898274778002843
1105	2	-0.001172618485810517
1105	3	0.04105395271443513
1105	4	-0.1282125279862285
1105	5	-0.11218877377698533
1105	6	-0.1473017552628022
1105	7	-0.4772052165818721
1105	8	-0.4052473394708187
1105	9	-0.3652513024735953
1105	10-11	-0.25057245315331755
1105	12-13	-0.21795935892481566
1105	14-15	-0.12819111982452114
1105	16-17	-0.17064985867967408
1105	18-19	-0.20909342762504934
1105	20-21	-0.13413488699040954
1105	22-23	-0.12697545538527066
1105	24-25	-0.1489291978629268
1105	26-27	-0.1716230607873257
1105	28-29	-0.176432307405193
1105	30-31	-0.13123052745618935
1105	32-33	-0.14146342356396957
1105	34-35	-0.16478793191866004
1105	36-37	-0.1343969880559328
1105	38-39	-0.1695474253436302
1105	40-41	-0.23776382958811837
1105	42-43	-0.1744212167023491
1105	44-45	-0.21031196968815635
1105	46-47	-0.13996757896430978
1105	48-49	-0.09649253320017692
1105	50-51	-0.048639826943635
1105	52-53	-0.06471019202391659
1105	54-55	-0.07016643800017164
1105	56-57	-0.07742968972971198
1105	58-59	-0.0696713447824635
1105	60-61	-0.060483247103960025
1105	62-63	-0.07113770040471934
1105	64-65	-0.037040503496236
1105	66-67	-0.117609238165862
1105	68-69	-0.15387133352533766
1105	70-71	-0.14919856113699126
1105	72-73	-0.07018297377562988
1105	74-75	-0.09956348783900637
1105	76-77	-0.04719437723335318
1105	78-79	-0.06820429050808485
1105	80-81	-0.07611524333714925
1105	82-83	-0.0769023371253823
1105	84-85	-0.08611695360036009
1105	86-87	-0.11757755898553768
1105	88-89	-0.14263187806356115
1105	90-91	-0.15538275493423725
1105	92-93	-0.1293635277341565
1105	94-95	-0.16999201670937936
1105	96-97	-0.1110637887132242
1105	98-99	-0.12127302180246247
1105	100	-0.08588640995919405
1106	1	0.08244734849780855
1106	2	0.1011375547627793
1106	3	0.10393350541843915
1106	4	0.09567564982084065
1106	5	0.08407736716223013
1106	6	0.11146766799068075
1106	7	0.09429217716178329
1106	8	0.11595498198511933
1106	9	0.0876573785459911
1106	10-11	0.10584968481828838
1106	12-13	0.09304727278998826
1106	14-15	0.1599028519999237
1106	16-17	0.18708706850495815
1106	18-19	0.14979947276167138
1106	20-21	0.10619065759705393
1106	22-23	0.1328240244363883
1106	24-25	0.10775755816701604
1106	26-27	0.09602673217699476
1106	28-29	0.09407373425537457
1106	30-31	0.09078191151252923
1106	32-33	0.07948864578199277
1106	34-35	0.11620674285467203
1106	36-37	0.14152370603621733
1106	38-39	0.12026944382606075
1106	40-41	0.16937609192546432
1106	42-43	0.1665753148020528
1106	44-45	0.08029928447381707
1106	46-47	0.12924931082820024
1106	48-49	0.12189930278496064
1106	50-51	0.11285529783851445
1106	52-53	0.0724483385880319
1106	54-55	0.08291681413882657
1106	56-57	0.11520367331056747
1106	58-59	0.05999480164562243
1106	60-61	0.034683311498099556
1106	62-63	0.0919313314783139
1106	64-65	0.10084596959681136
1106	66-67	0.09971440146522781
1106	68-69	0.057130769301643625
1106	70-71	0.07990696319132695
1106	72-73	0.10636054968327358
1106	74-75	0.08982125089329429
1106	76-77	0.08277468515473174
1106	78-79	0.055982906834309176
1106	80-81	0.028544022230839516
1106	82-83	0.059310536865027075
1106	84-85	0.06046255588155702
1106	86-87	0.01684196578392516
1106	88-89	0.03102570481367195
1106	90-91	0.032590100199740846
1106	92-93	0.05665680076042179
1106	94-95	0.06992956463819411
1106	96-97	0.061867500604670056
1106	98-99	0.046278231146533955
1106	100	0.08021069730124353
1107	1	-0.012691429129660037
1107	2	0.023197719585205334
1107	3	0.05419265704902898
1107	4	-0.03461943809666934
1107	5	-0.04445766909712745
1107	6	-0.04156691465964002
1107	7	-0.011412966637038835
1107	8	0.014325675864142795
1107	9	0.041457577084763386
1107	10-11	0.0223017269298893
1107	12-13	0.09982801384069262
1107	14-15	0.07555717416786933
1107	16-17	0.0741546841013303
1107	18-19	0.06198850143879753
1107	20-21	0.04532172336459439
1107	22-23	0.031966451191038914
1107	24-25	0.048737878279894176
1107	26-27	0.05110681913543402
1107	28-29	0.06310490678662006
1107	30-31	0.05784309009391819
1107	32-33	0.07059197405092732
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2216	30-31	-0.5403592576091043
2216	32-33	-0.531034851661019
2216	34-35	-0.4911534054707971
2216	36-37	-0.5495643459657416
2216	38-39	-0.5909305946737149
2216	40-41	-0.5757252040707712
2216	42-43	-0.581516046364321
2216	44-45	-0.4966206911825779
2216	46-47	-0.45325322030527104
2216	48-49	-0.4632207155635726
2216	50-51	-0.43953157499412754
2216	52-53	-0.4885896479782659
2216	54-55	-0.5650483833874063
2216	56-57	-0.5140544478023372
2216	58-59	-0.6295717404382657
2216	60-61	-0.6030748501408851
2216	62-63	-0.6374366030958143
2216	64-65	-0.5893732695329419
2216	66-67	-0.480448106386703
2216	68-69	-0.5125881403789876
2216	70-71	-0.4888647890098383
2216	72-73	-0.5995392708839944
2216	74-75	-0.4805046766474632
2216	76-77	-0.44311718566208924
2216	78-79	-0.45212424029005405
2216	80-81	-0.4672328366063212
2216	82-83	-0.47745808085311836
2216	84-85	-0.4362882565616246
2216	86-87	-0.38091067068242523
2216	88-89	-0.33243063217329905
2216	90-91	-0.32475097291699484
2216	92-93	-0.451697110041664
2216	94-95	-0.48741813763762565
2216	96-97	-0.5546649496093892
2216	98-99	-0.4953459617380567
2216	100	-0.439911184897813
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	53.0
3	411.0
4	227.0
5	426.0
6	584.0
7	1010.0
8	1813.0
9	2721.0
10	3873.0
11	5545.0
12	8769.0
13	13140.0
14	13846.0
15	17304.0
16	21566.0
17	33199.0
18	41439.0
19	20727.0
20	11982.0
21	10255.0
22	10185.0
23	10728.0
24	12273.0
25	14819.0
26	18520.0
27	23562.0
28	29417.0
29	35908.0
30	45160.0
31	57047.0
32	71428.0
33	92114.0
34	128072.0
35	209985.0
36	336790.0
37	746863.0
38	1514841.0
39	981787.0
40	10478.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.00130899843741	22.263174278432984	15.091565175595987	23.64395154753362
2	13.015517455437383	22.887643549952994	41.26129173351443	22.835547261095194
3	14.449944624083184	30.17076050689199	32.59589443895938	22.78340043006545
4	9.872278445054505	19.57350104030824	42.02939624830957	28.524824266327688
5	10.710819157479259	39.27094166160144	36.843277068622534	13.174962112296761
6	25.750104137716672	42.04707002858385	19.362157307945154	12.840668525754314
7	22.63551448199739	34.93993134697722	25.89663177276284	16.52792239826255
8	23.429374008936872	35.625935128179876	22.635295129250316	18.30939573363294
9	25.02259881676741	15.94442215576809	23.23798873711385	35.794990290350654
10-11	21.729116028171035	27.75195021043606	31.229842853498468	19.28909090789444
12-13	23.522193123861694	26.330676021037686	30.766306628379375	19.380824226721245
14-15	20.976056112187525	27.162637120144105	29.471522200581855	22.389784567086515
16-17	18.958361803491965	30.419553805803062	29.58592562581887	21.0361587648861
18-19	18.827561760411086	28.398821461560512	33.53629311844368	19.237323659584717
20-21	20.3641825918589	27.643337697721826	33.09740117445848	18.8950785359608
22-23	19.734881350388896	27.70131685203629	33.1785689177645	19.385232879810314
24-25	19.446693116978196	27.88327132042258	33.1103098634802	19.559725699119024
26-27	19.353751139953683	27.814376330859936	33.15878629412368	19.6730862350627
28-29	19.39056910911513	27.65184991665619	33.41917778410981	19.53840319011886
30-31	19.87646082946883	28.048605805435074	32.806615429465275	19.268317935630826
32-33	19.22745559087658	27.848227885212406	33.275630017438225	19.648686506472785
34-35	19.552015692470057	27.622507208596815	33.09170682045299	19.73377027848014
36-37	20.000603242483187	27.952523719768642	32.334268724783776	19.712604312964395
38-39	19.782614513394847	28.005033377438814	32.55326877788034	19.659083331285995
40-41	19.677619998335057	27.85547564398292	32.48440707966349	19.98249727801853
42-43	19.54351186966532	27.93082813903863	32.50039264305368	20.025267348242362
44-45	19.886017281993766	27.91389888105528	32.05767508421534	20.142408752735612
46-47	20.055042080034326	28.306253940273248	31.4678282859082	20.17087569378423
48-49	20.13831642445846	28.197411571154372	31.169651768576912	20.494620235810252
50-51	20.33518783491419	28.22024880577571	30.715501411879444	20.72906194743066
52-53	20.24779193944107	28.467184266259256	30.603253222019077	20.6817705722806
54-55	20.413565867709504	28.739326914081293	30.439803910579418	20.407303307629785
56-57	20.35990837116653	28.37893387926324	30.461311648807904	20.799846100762323
58-59	20.200786166626088	27.926770684583545	30.881487135953744	20.990956012836627
60-61	20.603483930306478	28.3716787506837	30.44440747114593	20.580429847863897
62-63	20.38907912865192	28.292874084723245	30.635967226067358	20.682079560557483
64-65	20.389090096289276	28.10446104262309	30.74126751230075	20.765181348786882
66-67	20.438612586849324	28.19059146651569	30.557909656415095	20.81288629021989
68-69	20.789754053500925	28.293871880043092	30.399222433265326	20.517151633190657
70-71	20.751442550778552	28.103269196810977	30.44792833146697	20.6973599209435
72-73	20.480297231909393	28.196473047180937	30.536551371509287	20.786678349400383
74-75	20.511968395035733	28.25187234954985	30.425613006995846	20.810546248418575
76-77	20.556820311791384	28.404151091190883	30.258796654726154	20.78023194229158
78-79	20.584484966165565	28.36740321008718	30.402086769440405	20.64602505430685
80-81	20.499739681214503	28.328222559564853	30.345789114693485	20.82624864452716
82-83	20.275879405637905	28.28148877221841	30.525027016409023	20.917604805734662
84-85	20.238402074576026	28.18536456106647	30.358836986222943	21.217396378134563
86-87	20.428664747763364	28.340828638074395	30.192368520685243	21.038138093477002
88-89	20.096220443855593	28.16619057307264	30.401854434360775	21.33573454871098
90-91	20.100039604768323	28.182560104431115	30.330178296038568	21.387221994761994
92-93	20.10073427304753	28.34419875814002	30.338966375806926	21.216100593005525
94-95	20.02276248671119	27.918232725533045	30.466986332966023	21.592018454789745
96-97	19.96727390223673	28.21695303555999	30.432814104401935	21.382958957801343
98-99	19.849577500194954	28.070596836705246	30.37166141359289	21.70816424950691
100	19.915037293477578	28.10218513762458	30.304849665672673	21.677927903225168
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6279.0
1	3820.0
2	4512.0
3	6061.0
4	2794.0
5	1535.5
6	3415.0
7	8999.5
8	15354.0
9	15395.0
10	8967.0
11	3893.5
12	3059.0
13	3212.0
14	3578.5
15	3976.0
16	4385.5
17	5103.0
18	5964.0
19	6957.5
20	8616.5
21	10889.0
22	13960.0
23	18694.0
24	25399.5
25	33540.0
26	44086.5
27	57397.5
28	73481.5
29	90408.0
30	106145.5
31	120788.0
32	133605.0
33	144394.5
34	153210.0
35	161099.0
36	167299.0
37	171623.0
38	174768.5
39	174105.5
40	173234.5
41	171937.5
42	167905.0
43	166942.5
44	164292.0
45	155568.5
46	147787.5
47	142025.5
48	134371.5
49	127877.5
50	124572.5
51	116322.5
52	102285.0
53	90426.5
54	81887.5
55	75501.0
56	67342.5
57	58584.0
58	53972.5
59	49721.0
60	43405.0
61	36521.0
62	29455.5
63	21744.0
64	15355.5
65	11573.5
66	8800.5
67	6326.5
68	4348.0
69	3067.0
70	2238.0
71	1866.0
72	1443.5
73	845.0
74	548.5
75	401.0
76	256.5
77	180.0
78	125.0
79	64.0
80	28.0
81	10.0
82	4.0
83	5.0
84	3.5
85	4.0
86	2.5
87	1.5
88	2.0
89	1.5
90	2.5
91	2.5
92	1.5
93	1.5
94	4.0
95	6.5
96	11.0
97	14.5
98	7.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009212815377154016
2	2.1935274707509564E-5
3	5.703171423952486E-4
4	0.0026761035143161665
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	5.483818676877391E-5
24-25	9.870873618379303E-5
26-27	4.2773785679643645E-4
28-29	8.774109883003825E-4
30-31	0.0015354692295256693
32-33	0.004463828402978196
34-35	0.005428980490108617
36-37	0.003718029062922871
38-39	0.0021715921960434466
40-41	0.0032354530193576606
42-43	4.1677021944268175E-4
44-45	3.9483494473517214E-4
46-47	0.0037948025243991546
48-49	0.002566427140778619
50-51	0.0010967637353754781
52-53	4.387054941501913E-5
54-55	6.251553291640225E-4
56-57	8.774109883003825E-4
58-59	8.116051641778539E-4
60-61	4.716084062114556E-4
62-63	0.0
64-65	0.0
66-67	8.225728015316087E-4
68-69	6.580582412252869E-4
70-71	0.0022373980201659755
72-73	5.483818676877391E-5
74-75	0.001963207086322106
76-77	3.8386730738141733E-4
78-79	0.0010967637353754781
80-81	7.348317027015704E-4
82-83	0.0013928899439268573
84-85	0.0012283753836205356
86-87	2.851585711976243E-4
88-89	0.0014148252186343669
90-91	0.0015903074162944432
92-93	0.0030819060964050937
94-95	0.001107731372729233
96-97	0.0026322329649011477
98-99	0.002358042031057278
100	0.00353157922790904
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4558867.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.9942569428341
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.28123194071163	44.41160340083432
2	15.519898634296373	18.311697755168403
3	4.64393937516014	8.218972581754253
4	1.8521741861081804	4.370705593526025
5	0.8568996167822468	2.5276078083333977
6	0.4796836505884631	1.6979148320455466
7	0.27831437305451984	1.149326474440349
8	0.19509973882573553	0.9207811297412214
9	0.12725349755081702	0.6756502978248489
>10	0.6586284727645689	7.119271647231183
>50	0.06211680782777312	2.5378932502481213
>100	0.041177785349322225	4.456464302599523
>500	0.0026864412175274252	1.0345961596091109
>1k	7.08921478677384E-4	0.7389559444718179
>5k	7.46233135449271E-5	0.357712700056768
>10k+	1.1193497031739065E-4	1.4708461221151494
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29914	0.656171807600441	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20501	0.44969506677865356	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16406	0.3598701168514019	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8738	0.19167043039421855	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7513	0.16479971887751935	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	6.58058241225287E-5	0.0
9	2.1935274707509564E-5	0.0	0.0	6.58058241225287E-5	0.0
10-11	6.58058241225287E-5	0.0	0.0	8.774109883003825E-5	0.0
12-13	6.58058241225287E-5	0.0	0.0	9.870873618379303E-5	0.0
14-15	1.0967637353754783E-4	0.0	0.0	1.0967637353754782E-4	0.0
16-17	1.316116482450574E-4	0.0	0.0	1.0967637353754782E-4	0.0
18-19	1.4257928559881217E-4	0.0	0.0	1.2064401089130261E-4	0.0
20-21	1.5354692295256695E-4	0.0	0.0	2.4128802178260522E-4	0.0
22-23	2.0838510972134085E-4	0.0	0.0	5.483818676877391E-4	0.0
24-25	2.632232964901148E-4	0.0	0.0	0.0011406342847904974	0.0
26-27	2.632232964901148E-4	0.0	0.0	0.002029012910444635	0.0
28-29	2.8515857119762434E-4	0.0	0.0	0.004112864007658043	0.0
30-31	3.070938459051339E-4	0.0	0.0	0.008774109883003825	0.0
32-33	3.6193203267390777E-4	0.0	0.0	0.017098546634503706	0.0
34-35	3.9483494473517214E-4	0.0	0.0	0.02885585387772883	0.0
36-37	3.9483494473517214E-4	0.0	0.0	0.04592149760017127	0.0
38-39	4.167702194426817E-4	0.0	0.0	0.07081803439319462	0.0
40-41	4.167702194426817E-4	0.0	0.0	0.0995642118973859	0.0
42-43	4.167702194426817E-4	0.0	0.0	0.12942908841166018	0.0
44-45	4.167702194426817E-4	0.0	0.0	0.1627707059670747	0.0
46-47	4.167702194426817E-4	0.0	0.0	0.20142066000170655	0.0
48-49	4.606407688577008E-4	0.0	0.0	0.24385444892338382	0.0
50-51	4.825760435652104E-4	0.0	0.0	0.2901598138309365	0.0
52-53	5.045113182727199E-4	0.0	0.0	0.3362787289034753	0.0
54-55	5.045113182727199E-4	0.0	0.0	0.38624728468718217	0.0
56-57	5.154789556264747E-4	0.0	0.0	0.44661316068224843	0.0
58-59	5.374142303339843E-4	0.0	0.0	0.5172995834271981	0.0
60-61	5.703171423952487E-4	0.0	0.0	0.594544653309693	0.0
62-63	5.922524171027582E-4	0.0	0.0	0.6725574578069506	0.0
64-65	6.141876918102678E-4	0.0	0.0	0.756361174826991	0.0
66-67	6.141876918102678E-4	0.0	0.0	0.8491144839276952	0.0
68-69	6.141876918102678E-4	0.0	0.0	0.9550289578529052	0.0
70-71	6.580582412252869E-4	0.0	0.0	1.078524554456184	0.0
72-73	6.580582412252869E-4	0.0	0.0	1.2121542479743321	0.0
74-75	6.909611532865512E-4	0.0	0.0	1.3498529349507233	0.0
76-77	7.457993400553252E-4	0.0	0.0	1.4995173142800613	0.0
78-79	8.116051641778538E-4	0.0	0.0	1.666181531507719	0.0
80-81	8.335404388853634E-4	0.0	0.0	1.8585100201431628	0.0
82-83	8.335404388853634E-4	0.0	0.0	2.0674435117321917	0.0
84-85	8.335404388853634E-4	0.0	0.0	2.286708517708457	0.0
86-87	8.335404388853634E-4	0.0	0.0	2.5142760251615153	0.0
88	8.335404388853634E-4	0.0	0.0	2.6973149249583286	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9335	0.0	60.725212	1
GTATCAA	20315	0.0	48.334545	1
ATCAACG	26465	0.0	36.869137	3
TCAACGC	26750	0.0	36.528633	4
CAACGCA	27340	0.0	35.704784	5
TATCAAC	28420	0.0	34.547916	2
AACGCAG	28370	0.0	34.458187	6
ACGCAGA	31865	0.0	30.501768	7
CGCAGAG	31865	0.0	30.442772	8
GCAGAGT	34160	0.0	28.067307	9
GTACATG	16355	0.0	24.74507	1
TACATGG	15965	0.0	24.464682	2
ACATGGG	16005	0.0	23.405079	3
AGAGTAC	33225	0.0	23.21304	10-11
GAGTACT	25770	0.0	22.113413	12-13
AGTACTT	26980	0.0	21.966536	12-13
CAGAGTA	34340	0.0	21.590242	10-11
CATGGGT	4105	0.0	20.380253	4
ATAACGC	490	0.0	20.143305	3
GTGGTAT	3605	0.0	19.818665	1
>>END_MODULE