FastQCFastQC Report
Wed 25 May 2016
SRR2431369_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431369_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6097386
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA223320.36625531006237755No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63500.049.2944831
GTATCAA119700.041.777771
TCAACGC137000.035.1296354
ATCAACG139350.034.6383933
CAACGCA146950.032.7829865
AACGCAG153400.031.251376
TATCAAC169050.028.9976922
GTACATG279000.027.745161
TACATGG266550.027.4362752
ACGCAGA171850.027.4312537
CGCAGAG172900.026.8841068
ACATGGG276450.025.6036953
CATGGGT73700.023.0215194
GCAGAGT199700.022.1936059
CATGGGG145250.021.12964
AGAGTAC173650.018.83773610-11
CAGAGTA189400.018.22661810-11
ATGGGTA40050.017.2507925
CATGGGA78950.017.0853564
CATGGGC67300.016.4812894
Produced by FastQC (version 0.11.5)