##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431369_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6097386
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.300213730933223	33.0	31.0	34.0	28.0	34.0
2	31.332777521383754	34.0	31.0	34.0	28.0	34.0
3	31.410502959792936	34.0	31.0	34.0	28.0	34.0
4	34.62844241778362	37.0	35.0	37.0	32.0	37.0
5	34.759982884468855	37.0	35.0	37.0	32.0	37.0
6	34.79952261510096	37.0	35.0	37.0	32.0	37.0
7	34.70106370172398	37.0	35.0	37.0	32.0	37.0
8	34.65393022518174	37.0	35.0	37.0	32.0	37.0
9	36.150847100708404	39.0	37.0	39.0	32.0	39.0
10-11	36.18000984356247	39.0	37.0	39.0	32.0	39.0
12-13	36.146045124910906	39.0	37.0	39.0	32.0	39.0
14-15	37.54530187198252	41.0	38.0	41.0	32.0	41.0
16-17	37.56729031096276	41.0	38.0	41.0	32.0	41.0
18-19	37.60468321998968	41.0	38.5	41.0	32.0	41.0
20-21	37.66435690638579	41.0	39.0	41.0	32.0	41.0
22-23	37.68009496200503	41.0	39.0	41.0	31.0	41.0
24-25	37.715083480035545	41.0	38.0	41.0	31.0	41.0
26-27	37.72283221039311	41.0	38.0	41.0	31.0	41.0
28-29	37.699781758937355	41.0	38.0	41.0	31.0	41.0
30-31	37.635208923955275	41.0	38.0	41.0	31.0	41.0
32-33	37.55958782993237	40.0	38.0	41.0	30.0	41.0
34-35	37.49743053826673	40.0	38.0	41.0	30.0	41.0
36-37	37.40281589520493	40.0	38.0	41.0	30.0	41.0
38-39	37.30355934493896	40.0	38.0	41.0	30.0	41.0
40-41	37.22128818808584	40.0	38.0	41.0	30.0	41.0
42-43	37.1060444590518	40.0	38.0	41.0	30.0	41.0
44-45	36.982326934853724	40.0	38.0	41.0	30.0	41.0
46-47	36.863771540788136	40.0	37.0	41.0	30.0	41.0
48-49	36.736579576887536	40.0	37.0	41.0	30.0	41.0
50-51	36.613027861447506	40.0	37.0	41.0	30.0	41.0
52-53	36.458160185364676	40.0	36.0	41.0	29.0	41.0
54-55	36.30092206725964	40.0	36.0	41.0	29.0	41.0
56-57	36.1109381134801	40.0	35.5	41.0	29.0	41.0
58-59	35.89649105370728	39.0	35.0	41.0	29.0	41.0
60-61	35.72365945013158	39.0	35.0	41.0	28.0	41.0
62-63	35.5610592801571	39.0	35.0	41.0	28.0	41.0
64-65	35.3066099144781	39.0	35.0	41.0	28.0	41.0
66-67	34.99502754131032	38.0	35.0	40.0	28.0	41.0
68-69	34.66795894174979	37.5	34.0	40.0	27.0	41.0
70-71	34.39894153002615	37.0	34.0	40.0	26.5	41.0
72-73	34.13277927623411	36.5	34.0	39.5	26.0	41.0
74-75	33.778402335033405	36.0	34.0	39.0	26.0	41.0
76-77	33.416528082689865	36.0	34.0	39.0	26.0	40.5
78-79	33.06338117678625	35.0	34.0	37.5	26.0	39.5
80-81	32.70797305599481	35.0	33.5	37.0	25.0	39.0
82-83	32.35935956818217	35.0	33.0	37.0	25.0	39.0
84-85	32.058357548628216	35.0	33.0	36.0	24.5	38.0
86-87	31.78136647409234	35.0	33.0	36.0	24.0	37.0
88-89	31.51810464353085	35.0	33.0	35.5	23.5	37.0
90-91	31.30141744347496	35.0	33.0	35.0	23.0	36.5
92-93	31.093838818798744	35.0	33.0	35.0	21.5	36.0
94-95	30.878892036685883	35.0	32.0	35.0	19.5	36.0
96-97	30.690589869166885	35.0	32.0	35.0	18.5	36.0
98-99	30.561174985477383	35.0	32.0	35.0	9.5	35.5
100	30.514386820844212	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3402569237698323
1101	2	0.36931620503784046
1101	3	0.4243244811567166
1101	4	0.3648871696621754
1101	5	0.26772800252408757
1101	6	0.34399637713911346
1101	7	0.31588888989841024
1101	8	0.33049554648518864
1101	9	0.43716021216658163
1101	10-11	0.4328254607469546
1101	12-13	0.4394659002984298
1101	14-15	0.4968459331821151
1101	16-17	0.5010062118429275
1101	18-19	0.466199409776479
1101	20-21	0.4327370498287735
1101	22-23	0.4124931377306851
1101	24-25	0.45676844467543276
1101	26-27	0.48310501397200767
1101	28-29	0.5254703933085239
1101	30-31	0.560379774817271
1101	32-33	0.552383252796254
1101	34-35	0.5629184799339484
1101	36-37	0.5535791608859384
1101	38-39	0.5287300196249589
1101	40-41	0.555370472560341
1101	42-43	0.5623713774613677
1101	44-45	0.5688513369223926
1101	46-47	0.5453712543760219
1101	48-49	0.5135642215592995
1101	50-51	0.5211979883928706
1101	52-53	0.5682550497140966
1101	54-55	0.5850679320210261
1101	56-57	0.5139006075940813
1101	58-59	0.5456641381492844
1101	60-61	0.5442269979140804
1101	62-63	0.5569947745674639
1101	64-65	0.5897627513809525
1101	66-67	0.5621666203827118
1101	68-69	0.6177745725740351
1101	70-71	0.6004043539459261
1101	72-73	0.6022408519975571
1101	74-75	0.6049441572148808
1101	76-77	0.5573284359071025
1101	78-79	0.5482839610447812
1101	80-81	0.5361595811775786
1101	82-83	0.5496474808415499
1101	84-85	0.5130325026174347
1101	86-87	0.4944300413556313
1101	88-89	0.5067386022646048
1101	90-91	0.5161411425363269
1101	92-93	0.562242467779825
1101	94-95	0.6341358156320247
1101	96-97	0.5953710627183675
1101	98-99	0.6173304100480266
1101	100	0.6306330584271791
1102	1	0.18610270180856858
1102	2	0.22444478096361564
1102	3	0.23883846514035767
1102	4	0.22987404127996314
1102	5	0.16352635606945398
1102	6	0.19978005956095046
1102	7	0.21786390597933547
1102	8	0.20272682295671984
1102	9	0.27905143091880547
1102	10-11	0.2781335205481241
1102	12-13	0.23607255086518109
1102	14-15	0.32355866788043386
1102	16-17	0.3607968045393335
1102	18-19	0.2958399317815932
1102	20-21	0.26331564456683054
1102	22-23	0.23370278376260245
1102	24-25	0.3197143050123188
1102	26-27	0.2606077989496214
1102	28-29	0.28083137047706686
1102	30-31	0.27717391593040475
1102	32-33	0.27269917432222
1102	34-35	0.3175705613276847
1102	36-37	0.347211112130843
1102	38-39	0.29016169361210586
1102	40-41	0.3021488271066488
1102	42-43	0.3102015274524632
1102	44-45	0.2809639884847144
1102	46-47	0.303055189691257
1102	48-49	0.24483521920686968
1102	50-51	0.2812864873960734
1102	52-53	0.2757944186625281
1102	54-55	0.26716424599759847
1102	56-57	0.3048487572987426
1102	58-59	0.30778859347000065
1102	60-61	0.32998422388647697
1102	62-63	0.3531760960477257
1102	64-65	0.3417228623019284
1102	66-67	0.3632005740410449
1102	68-69	0.32910811743384727
1102	70-71	0.3154242727358394
1102	72-73	0.29635641661518264
1102	74-75	0.3083765150464046
1102	76-77	0.2873487630040046
1102	78-79	0.23251350447378627
1102	80-81	0.295456482691705
1102	82-83	0.2544372639059773
1102	84-85	0.2622524952377461
1102	86-87	0.25776757617343904
1102	88-89	0.3290583503699125
1102	90-91	0.2783510714227688
1102	92-93	0.2561112817614202
1102	94-95	0.2356795427967171
1102	96-97	0.25487520935229924
1102	98-99	0.26563500605423584
1102	100	0.2718936467601196
1103	1	0.18022824965846596
1103	2	0.13861197001986625
1103	3	0.17422867862466518
1103	4	0.16384276184405167
1103	5	0.09215121245351554
1103	6	0.17800069846483524
1103	7	0.18546561704417286
1103	8	0.22782500583196708
1103	9	0.23130378694527565
1103	10-11	0.18530334643583757
1103	12-13	0.26103403905258205
1103	14-15	0.2966353966933397
1103	16-17	0.24518762247947734
1103	18-19	0.23064350753006835
1103	20-21	0.21307859587352596
1103	22-23	0.22676896838068927
1103	24-25	0.20384510230078234
1103	26-27	0.20945617710299302
1103	28-29	0.24816189625477136
1103	30-31	0.23843139500021238
1103	32-33	0.1578050027068585
1103	34-35	0.26440652424165023
1103	36-37	0.2837852919462378
1103	38-39	0.2405507502589188
1103	40-41	0.23258956406816367
1103	42-43	0.22468672322825967
1103	44-45	0.2658824405043063
1103	46-47	0.1802745214676449
1103	48-49	0.2280330608315282
1103	50-51	0.18288445983235846
1103	52-53	0.19761461513374456
1103	54-55	0.15262447946124524
1103	56-57	0.11841158115556993
1103	58-59	0.10043461701412326
1103	60-61	0.09240257373522809
1103	62-63	0.14365218411766278
1103	64-65	0.1680034006697113
1103	66-67	0.12751975457163667
1103	68-69	0.1362846668215738
1103	70-71	0.07865609605017454
1103	72-73	0.11886969387979462
1103	74-75	0.1872170878424626
1103	76-77	0.1630004316514686
1103	78-79	0.15468913597193534
1103	80-81	0.13731477734842912
1103	82-83	0.15126826659075476
1103	84-85	0.14208791532795573
1103	86-87	0.1390270228557533
1103	88-89	0.16092715955895898
1103	90-91	0.12899993037230928
1103	92-93	0.13625469747740127
1103	94-95	0.09309252290900005
1103	96-97	0.09214673394547646
1103	98-99	0.0036842731597772627
1103	100	-0.04781701862248866
1104	1	0.06490536854448692
1104	2	0.07403884577858832
1104	3	0.08255245708801695
1104	4	0.06555589312539922
1104	5	0.028130067916549706
1104	6	0.053619828416906046
1104	7	0.021895220841919638
1104	8	0.10653903573388845
1104	9	0.12644341397543002
1104	10-11	0.11162357006249835
1104	12-13	0.13011053112482784
1104	14-15	0.12234549107332526
1104	16-17	0.1454157515797263
1104	18-19	0.07470113715085347
1104	20-21	0.08496547701817292
1104	22-23	0.04511977696639491
1104	24-25	0.10569969451966443
1104	26-27	0.13313460481055017
1104	28-29	0.15355384613827283
1104	30-31	0.19606382434611191
1104	32-33	0.18016899969458677
1104	34-35	0.14215237498896016
1104	36-37	0.18763610813929432
1104	38-39	0.20664056536139697
1104	40-41	0.19389683951024494
1104	42-43	0.1821026708614184
1104	44-45	0.1426586224072608
1104	46-47	0.14808037969835652
1104	48-49	0.12256221300699366
1104	50-51	0.07131079012628305
1104	52-53	0.12950770848322435
1104	54-55	0.07144010302531001
1104	56-57	0.10968331021699385
1104	58-59	0.10451201700260526
1104	60-61	0.1457132264429859
1104	62-63	0.09065382996661242
1104	64-65	0.22498379480348518
1104	66-67	0.17450629695289877
1104	68-69	0.13846038847793807
1104	70-71	0.10845251266704992
1104	72-73	0.054170224086199426
1104	74-75	0.12302623785053157
1104	76-77	0.08643897958613422
1104	78-79	0.10385192438033641
1104	80-81	0.09884806851758299
1104	82-83	0.1308646615733764
1104	84-85	0.11855614062323383
1104	86-87	0.10413618513488743
1104	88-89	0.13037259017801262
1104	90-91	0.0811234870665416
1104	92-93	0.11956363763783884
1104	94-95	0.1260579267120434
1104	96-97	0.1760609767578316
1104	98-99	0.18404893490665586
1104	100	0.20686033901113987
1105	1	0.09250236637955567
1105	2	0.15387483556352066
1105	3	0.14623832912492674
1105	4	0.18692454736291353
1105	5	0.17088987801396627
1105	6	0.12189769821017649
1105	7	0.1257788382154601
1105	8	0.13163061943060228
1105	9	0.11874487178182846
1105	10-11	0.1527671565425095
1105	12-13	0.1902961105279175
1105	14-15	0.22130194159679206
1105	16-17	0.20576084328903477
1105	18-19	0.198734013273274
1105	20-21	0.19534857670140582
1105	22-23	0.20752369666930548
1105	24-25	0.2113874476100719
1105	26-27	0.18332964906676352
1105	28-29	0.2456232864107193
1105	30-31	0.24479509559034796
1105	32-33	0.2530295670567497
1105	34-35	0.25482110645828016
1105	36-37	0.27318531178759287
1105	38-39	0.23683450969933517
1105	40-41	0.218064644845974
1105	42-43	0.2188112757370746
1105	44-45	0.17661696859880038
1105	46-47	0.1750764284070243
1105	48-49	0.19239178485737085
1105	50-51	0.1586720809504385
1105	52-53	0.1826054781331976
1105	54-55	0.16346016373097427
1105	56-57	0.1190092845656352
1105	58-59	0.14664162465668795
1105	60-61	0.17185987816010595
1105	62-63	0.20770977389557999
1105	64-65	0.189654013175506
1105	66-67	0.17315807172457198
1105	68-69	0.10906539466014209
1105	70-71	0.14455952424747665
1105	72-73	0.14420602336625166
1105	74-75	0.139564628360084
1105	76-77	0.11902081751172489
1105	78-79	0.06853301310134441
1105	80-81	0.07566517719075705
1105	82-83	0.16268557506999315
1105	84-85	0.1968278670589001
1105	86-87	0.1683076195567459
1105	88-89	0.13845497647244542
1105	90-91	0.13269493893732687
1105	92-93	0.08639284823027538
1105	94-95	0.13446149155844722
1105	96-97	0.17092910513214576
1105	98-99	0.18952686610531444
1105	100	0.23291113035442734
1106	1	0.2994714311148421
1106	2	0.3349597033121192
1106	3	0.3231637264658431
1106	4	0.3044017847094125
1106	5	0.27631256765067747
1106	6	0.3171911793527329
1106	7	0.28184423526594315
1106	8	0.3328862042376812
1106	9	0.3908500902516252
1106	10-11	0.33340795986106286
1106	12-13	0.37982574920504675
1106	14-15	0.4377317436205672
1106	16-17	0.42574607019341926
1106	18-19	0.44471264281234113
1106	20-21	0.41113729183149417
1106	22-23	0.39538035640146774
1106	24-25	0.44735054518510964
1106	26-27	0.46285977167654835
1106	28-29	0.47883264328734043
1106	30-31	0.42247329470631456
1106	32-33	0.4409665095519486
1106	34-35	0.4016198030138227
1106	36-37	0.4595358661229838
1106	38-39	0.42211300519197437
1106	40-41	0.37391048079986433
1106	42-43	0.3946586301900723
1106	44-45	0.38130557851769
1106	46-47	0.3578005497335255
1106	48-49	0.3633687046238592
1106	50-51	0.33948359472087475
1106	52-53	0.32624082546363553
1106	54-55	0.39197826424156545
1106	56-57	0.3505549815994726
1106	58-59	0.3262581525589283
1106	60-61	0.30815865271786436
1106	62-63	0.39188075094875074
1106	64-65	0.4272705875525773
1106	66-67	0.4430774175950063
1106	68-69	0.4313373729685779
1106	70-71	0.44675656833499033
1106	72-73	0.39395953089018576
1106	74-75	0.4526951391527163
1106	76-77	0.4311834058229209
1106	78-79	0.4240195547868453
1106	80-81	0.42034340892040234
1106	82-83	0.4094022622558384
1106	84-85	0.3998181579519766
1106	86-87	0.34321049856727015
1106	88-89	0.34334576707649234
1106	90-91	0.32389187743375913
1106	92-93	0.4041606700476166
1106	94-95	0.4024408697002855
1106	96-97	0.42096336728457473
1106	98-99	0.4368305042688334
1106	100	0.4441944502579638
1107	1	0.11502145885486215
1107	2	0.16246893648353833
1107	3	0.1404220021461171
1107	4	0.0807509226612595
1107	5	0.08159482508101945
1107	6	0.1032833739073169
1107	7	0.015754348149151554
1107	8	0.09116533865589105
1107	9	0.08212727097962613
1107	10-11	0.14986742314365387
1107	12-13	0.16385048718145612
1107	14-15	0.2091363130506707
1107	16-17	0.18250924127008972
1107	18-19	0.16480391911794356
1107	20-21	0.16869525583241796
1107	22-23	0.22599891514582993
1107	24-25	0.18980411385056328
1107	26-27	0.2644030990956949
1107	28-29	0.25321674491428325
1107	30-31	0.2575498225971984
1107	32-33	0.2646431211044131
1107	34-35	0.2654583938052042
1107	36-37	0.28689533292304503
1107	38-39	0.2810496191814522
1107	40-41	0.2258412882840375
1107	42-43	0.22323457846837158
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2216	58-59	-0.47685945085262205
2216	60-61	-0.43708527916846407
2216	62-63	-0.4846935159598118
2216	64-65	-0.5565512481153547
2216	66-67	-0.562549643453309
2216	68-69	-0.6019744350254399
2216	70-71	-0.6215139524486162
2216	72-73	-0.5949964239329546
2216	74-75	-0.571968803458283
2216	76-77	-0.5230930971864112
2216	78-79	-0.41493590241115896
2216	80-81	-0.45283434423363644
2216	82-83	-0.45910089608153726
2216	84-85	-0.4172295992251627
2216	86-87	-0.45084295332644686
2216	88-89	-0.49637128121982244
2216	90-91	-0.44278884677675734
2216	92-93	-0.5335453114758693
2216	94-95	-0.5720275570200926
2216	96-97	-0.6775407959723339
2216	98-99	-0.7193393632506613
2216	100	-0.6855665635854216
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	101395.0
3	22194.0
4	15643.0
5	13482.0
6	9726.0
7	9882.0
8	11478.0
9	11704.0
10	11571.0
11	12664.0
12	14916.0
13	16688.0
14	17976.0
15	18594.0
16	18545.0
17	18234.0
18	17133.0
19	15869.0
20	14689.0
21	15282.0
22	18754.0
23	29979.0
24	50627.0
25	63649.0
26	57204.0
27	43445.0
28	45732.0
29	55222.0
30	66224.0
31	78708.0
32	101769.0
33	136063.0
34	194822.0
35	304951.0
36	547222.0
37	1113648.0
38	1850429.0
39	947629.0
40	3644.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.78959002440891	21.86966726435674	13.732821877260712	22.607920833973637
2	14.553923160439403	22.682890028176036	40.94320839442515	21.819978416959415
3	16.611995670929645	29.56112483011154	31.08097359145719	22.74590590750163
4	11.331457117647252	19.49935874096743	40.79791648216119	28.371267659224124
5	11.400452095744058	40.96109460766844	35.77860724137133	11.859846055216165
6	26.73366914936991	41.74372427791188	19.283328954407676	12.239277618310535
7	23.995807383688682	35.598172725164524	24.393338391238476	16.01268149990832
8	22.931925254527105	38.28061730059405	21.85900646604955	16.928450978829286
9	24.782423156414897	18.50461820852411	21.891823807775985	34.82113482728501
10-11	21.90334513839209	29.31742389279603	30.07789731534136	18.70133365347052
12-13	22.855269454812273	27.78447190320573	29.27008393432858	20.09017470765341
14-15	20.805087622794424	28.831051535854872	27.065352267348665	23.29850857400204
16-17	20.173349694442834	31.785702922531065	27.313392985125102	20.727554397901002
18-19	20.19599874437997	30.841700033424157	29.169253840908222	19.793047381287654
20-21	21.91578489536336	29.807223948098414	28.39269155667691	19.88429959986132
22-23	21.347459386694563	29.881600082395966	28.485879030784666	20.285061500124808
24-25	20.98741985500016	30.133011096886435	28.303136786813234	20.57643226130017
26-27	20.741626001699746	30.08478551300508	28.336683949482616	20.836904535812558
28-29	20.817141968705936	29.86785648800978	28.663110716625123	20.651890826659162
30-31	21.157779743647524	30.155463341176038	28.296765203974296	20.389991711202143
32-33	20.537021930381314	29.89435144830916	28.771444025357752	20.797182595951774
34-35	20.947812718433767	29.664679257635978	28.674172834063643	20.71333518986661
36-37	21.27829040182137	29.89604069678383	28.148176940085474	20.67749196130932
38-39	20.96240093705729	29.92907944486375	28.40964964330616	20.698869974772798
40-41	20.76439805516659	29.756398889622538	28.686489587505204	20.79271346770567
42-43	20.72914524355191	29.68275257626793	28.67218837711767	20.91591380306249
44-45	20.82202930895305	29.71298684387047	28.541263420095103	20.923720427081374
46-47	21.01895796001762	29.844936830307283	28.42071996098	20.7153852486951
48-49	20.87647067120238	29.593903026641254	28.642167315633287	20.88745898652308
50-51	20.93881706029436	29.679816892025535	28.297970638565445	21.08339540911466
52-53	20.871312723189906	29.679087071082595	28.379923790293088	21.06967641543442
54-55	20.971437596373267	30.01869161637462	28.249285841506506	20.760584945745602
56-57	20.94149033700671	29.748362659014866	28.248433017033857	21.061713986944568
58-59	20.985706005819544	29.341680188854703	28.409879249894953	21.262734555430804
60-61	21.19227813361332	29.74176146958713	28.20179827880341	20.864162117996138
62-63	20.850549727374975	29.72172009447983	28.41161769978151	21.01611247836368
64-65	20.95184723420823	29.603489101723262	28.427263748760534	21.01739991530797
66-67	20.875552244847217	29.515172567391996	28.23231955464194	21.37695563311885
68-69	21.241750153262398	29.784796304514753	28.104215478567372	20.869238063655473
70-71	21.233418714183422	29.634026778032425	28.231835740758417	20.90071876702574
72-73	21.015997675069283	29.614715223868064	28.34887769939446	21.020409401668193
74-75	20.992036587481913	29.622751454475736	28.354166851171964	21.031045106870387
76-77	21.051594896567153	29.7955796139526	28.084206904401327	21.06861858507892
78-79	21.20566911788101	29.853333871268774	28.120837355548755	20.820159655301467
80-81	21.06789696437129	29.7949317953628	28.22825223792622	20.90891900233969
82-83	20.91801762315028	29.836023765955115	28.383940472768828	20.862018138125777
84-85	21.012267976615366	29.62049176214276	28.176812569682046	21.190427691559822
86-87	21.178588497010644	29.806371736220385	28.05509715608641	20.95994261068256
88-89	20.965318551415336	29.63796864090754	28.245283597545683	21.15142921013144
90-91	20.87912087912088	29.597493286907863	28.231093558506714	21.292292275464543
92-93	20.94917740489435	29.767435643412526	28.244275685539655	21.03911126615347
94-95	20.939666088865295	29.49993365862747	28.316326488773385	21.244073763733855
96-97	20.862923107807045	29.794781725049134	28.094338706672367	21.24795646047145
98-99	20.66676490797504	29.69786369436686	28.25089550888793	21.384475888770176
100	20.79931916445773	29.771662926707183	28.102556347866418	21.326461560968664
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	19142.0
1	13728.0
2	6967.0
3	4613.5
4	3049.5
5	2318.5
6	2107.5
7	2326.0
8	2806.5
9	2865.0
10	2269.5
11	1769.5
12	1678.0
13	1738.0
14	1959.5
15	2165.5
16	2470.0
17	3209.5
18	4267.0
19	5568.0
20	7736.0
21	11018.5
22	15707.5
23	22507.0
24	31098.5
25	41579.0
26	54863.0
27	71569.0
28	91698.5
29	113149.5
30	131768.5
31	148371.5
32	165044.5
33	179099.0
34	191528.5
35	204563.0
36	214788.0
37	223053.5
38	232052.0
39	235691.0
40	236099.5
41	235179.5
42	229301.0
43	224427.5
44	218156.5
45	208310.0
46	200539.0
47	192680.0
48	180272.5
49	172487.0
50	168452.5
51	158455.5
52	145776.0
53	135197.0
54	126630.5
55	118252.0
56	106484.5
57	93503.0
58	84189.5
59	75738.0
60	66246.0
61	55115.5
62	44664.0
63	35657.0
64	28065.0
65	22063.0
66	17224.0
67	13251.0
68	10079.5
69	7556.5
70	5638.5
71	4421.0
72	3283.5
73	2273.0
74	1662.5
75	1263.0
76	892.5
77	655.5
78	508.0
79	379.5
80	300.5
81	232.5
82	194.5
83	170.0
84	139.5
85	117.5
86	103.5
87	90.0
88	78.0
89	66.0
90	56.5
91	55.5
92	56.5
93	41.5
94	26.5
95	29.0
96	33.5
97	25.0
98	23.0
99	37.5
100	85.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	3.772108244418182E-4
2	9.840282376743084E-5
3	4.920141188371542E-5
4	9.840282376743084E-5
5	8.20023531395257E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	1.3940400033719366E-4
84-85	7.872225901394467E-4
86-87	0.0013776395327440318
88-89	0.003394897419976364
90-91	0.0025994745945229643
92-93	0.002091060005057905
94-95	0.0022140635347671937
96-97	0.0020008574166044267
98-99	0.002533872712011344
100	0.002820880947999684
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6097386.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.91836611132505
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.81711340451515	43.491837128484235
2	15.655413310632413	18.447827461199033
3	5.196312971929365	9.184748103274856
4	2.160503640302984	5.0917337785688686
5	1.0880031138450719	3.20516828958928
6	0.5883603615344768	2.0799138711767924
7	0.36049815050086637	1.486797340956592
8	0.22612024167024944	1.0658108147127243
9	0.16965658212489812	0.8996299756947749
>10	0.6562268554062787	6.673245461146913
>50	0.045030674057608494	1.8417627205915412
>100	0.03232983395596221	3.754630811613106
>500	0.0029539265339876292	1.1903010989336797
>1k	0.0014490663305655957	1.2199333962696024
>5k	0.0	0.0
>10k+	2.7866660168386707E-5	0.36665974778805005
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	22332	0.36625531006237755	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	4.920141188371541E-5	0.0
5	0.0	0.0	0.0	4.920141188371541E-5	0.0
6	0.0	0.0	0.0	4.920141188371541E-5	0.0
7	0.0	0.0	0.0	8.20023531395257E-5	0.0
8	0.0	0.0	0.0	8.20023531395257E-5	0.0
9	0.0	0.0	0.0	9.840282376743083E-5	0.0
10-11	0.0	0.0	0.0	9.840282376743083E-5	0.0
12-13	0.0	0.0	0.0	1.066030590813834E-4	0.0
14-15	0.0	0.0	0.0	1.2300352970928853E-4	0.0
16-17	0.0	0.0	0.0	1.640047062790514E-4	0.0
18-19	0.0	0.0	0.0	1.886054122209091E-4	0.0
20-21	0.0	0.0	0.0	1.9680564753486165E-4	0.0
22-23	0.0	0.0	0.0	3.772108244418182E-4	0.0
24-25	0.0	0.0	0.0	7.380211782557313E-4	0.0
26-27	0.0	1.640047062790514E-5	0.0	0.0014432414152556522	0.0
28-29	0.0	1.640047062790514E-5	0.0	0.003739307303162372	0.0
30-31	0.0	1.640047062790514E-5	0.0	0.008979257668778063	0.0
32-33	0.0	1.640047062790514E-5	0.0	0.01890154239866067	0.0
34-35	0.0	1.640047062790514E-5	0.0	0.0322269247838336	0.0
36-37	0.0	1.640047062790514E-5	0.0	0.05279311495122664	0.0
38-39	0.0	1.640047062790514E-5	0.0	0.08178914702136292	0.0
40-41	0.0	1.640047062790514E-5	0.0	0.11406527321708024	0.0
42-43	0.0	1.640047062790514E-5	0.0	0.14482435587971632	0.0
44-45	0.0	1.640047062790514E-5	0.0	0.18302105197210738	0.0
46-47	0.0	1.640047062790514E-5	0.0	0.2279009398453698	0.0
48-49	0.0	1.640047062790514E-5	0.0	0.2767825425518411	0.0
50-51	0.0	8.20023531395257E-5	0.0	0.3299938695040793	0.0
52-53	8.20023531395257E-6	9.840282376743083E-5	0.0	0.38240157339555014	0.0
54-55	1.640047062790514E-5	9.840282376743083E-5	0.0	0.4390487989443345	0.0
56-57	1.640047062790514E-5	9.840282376743083E-5	0.0	0.504503077220304	0.0
58-59	1.640047062790514E-5	9.840282376743083E-5	0.0	0.5805766602278419	1.640047062790514E-5
60-61	1.640047062790514E-5	9.840282376743083E-5	0.0	0.6639566529001116	1.640047062790514E-5
62-63	1.640047062790514E-5	9.840282376743083E-5	0.0	0.7506413404039043	1.640047062790514E-5
64-65	1.640047062790514E-5	9.840282376743083E-5	0.0	0.8407783269748709	1.640047062790514E-5
66-67	1.640047062790514E-5	1.066030590813834E-4	0.0	0.9425432472210222	1.640047062790514E-5
68-69	1.640047062790514E-5	1.1480329439533597E-4	0.0	1.0599132152696256	1.640047062790514E-5
70-71	1.640047062790514E-5	1.1480329439533597E-4	0.0	1.1938886598289824	1.640047062790514E-5
72-73	1.640047062790514E-5	1.1480329439533597E-4	0.0	1.3386720145321291	1.640047062790514E-5
74-75	1.640047062790514E-5	1.1480329439533597E-4	0.0	1.4861942478301358	1.640047062790514E-5
76-77	1.640047062790514E-5	1.1480329439533597E-4	0.0	1.6420151192658625	1.640047062790514E-5
78-79	1.640047062790514E-5	1.1480329439533597E-4	0.0	1.8194272102832263	1.640047062790514E-5
80-81	1.640047062790514E-5	1.1480329439533597E-4	0.0	2.0256057267819356	1.640047062790514E-5
82-83	1.640047062790514E-5	1.1480329439533597E-4	0.0	2.250677585443992	1.640047062790514E-5
84-85	1.640047062790514E-5	1.1480329439533597E-4	0.0	2.4782833168180596	1.640047062790514E-5
86-87	1.640047062790514E-5	1.1480329439533597E-4	0.0	2.720157457638404	1.640047062790514E-5
88	1.640047062790514E-5	1.1480329439533597E-4	0.0	2.911575550571999	1.640047062790514E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6350	0.0	49.294483	1
GTATCAA	11970	0.0	41.77777	1
TCAACGC	13700	0.0	35.129635	4
ATCAACG	13935	0.0	34.638393	3
CAACGCA	14695	0.0	32.782986	5
AACGCAG	15340	0.0	31.25137	6
TATCAAC	16905	0.0	28.997692	2
GTACATG	27900	0.0	27.74516	1
TACATGG	26655	0.0	27.436275	2
ACGCAGA	17185	0.0	27.431253	7
CGCAGAG	17290	0.0	26.884106	8
ACATGGG	27645	0.0	25.603695	3
CATGGGT	7370	0.0	23.021519	4
GCAGAGT	19970	0.0	22.193605	9
CATGGGG	14525	0.0	21.1296	4
AGAGTAC	17365	0.0	18.837736	10-11
CAGAGTA	18940	0.0	18.226618	10-11
ATGGGTA	4005	0.0	17.250792	5
CATGGGA	7895	0.0	17.085356	4
CATGGGC	6730	0.0	16.481289	4
>>END_MODULE