FastQCFastQC Report
Wed 25 May 2016
SRR2431369_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431369_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6097386
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT362130.5939102428483288No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT245900.40328757274018734No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207100.3396537467039154No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA116500.063.9897421
GTATCAA253000.049.381871
ATCAACG342500.036.1173323
TCAACGC345250.035.8160364
CAACGCA356300.034.7181745
TATCAAC364950.033.9857142
AACGCAG367450.033.703056
ACGCAGA408000.030.1575647
CGCAGAG411000.029.8573918
GTACATG258900.027.8863891
TACATGG248650.027.747762
GCAGAGT446400.027.1738139
ACATGGG254300.026.336553
AGAGTAC424350.023.76244410-11
GTGGTAT38700.023.4411771
CATGGGG133950.023.1925814
AGTACTT322750.022.71669612-13
GAGTACT306000.022.6776912-13
CATGGGT69800.022.0182444
CAGAGTA443000.021.51017410-11
Produced by FastQC (version 0.11.5)