##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2431369_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6097386 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.76112993338457 34.0 31.0 34.0 31.0 34.0 2 32.88495119055936 34.0 31.0 34.0 31.0 34.0 3 32.959009319731436 34.0 31.0 34.0 31.0 34.0 4 36.34493305819904 37.0 37.0 37.0 35.0 37.0 5 36.2992780840839 37.0 37.0 37.0 35.0 37.0 6 36.331516161187764 37.0 37.0 37.0 35.0 37.0 7 36.1943906782349 37.0 37.0 37.0 35.0 37.0 8 36.24992627988453 37.0 37.0 37.0 35.0 37.0 9 38.06877799765342 39.0 39.0 39.0 35.0 39.0 10-11 38.05849072044971 39.0 39.0 39.0 35.0 39.0 12-13 38.038864441254006 39.0 39.0 39.0 35.0 39.0 14-15 39.522353595458775 41.0 39.5 41.0 36.0 41.0 16-17 39.542472298785086 41.0 39.5 41.0 37.0 41.0 18-19 39.620911239668935 41.0 40.0 41.0 37.0 41.0 20-21 39.62350604012933 41.0 40.0 41.0 37.0 41.0 22-23 39.54965996904247 41.0 40.0 41.0 36.5 41.0 24-25 39.521100845509864 41.0 40.0 41.0 36.0 41.0 26-27 39.43432415136585 41.0 39.5 41.0 36.0 41.0 28-29 39.34545106706382 41.0 39.0 41.0 36.0 41.0 30-31 39.17188308235693 41.0 39.0 41.0 35.5 41.0 32-33 38.966525819424916 41.0 39.0 41.0 35.0 41.0 34-35 38.86137411999175 41.0 39.0 41.0 35.0 41.0 36-37 38.74643396366902 41.0 38.5 41.0 35.0 41.0 38-39 38.622068456876434 41.0 38.0 41.0 35.0 41.0 40-41 38.52382947381058 41.0 38.0 41.0 35.0 41.0 42-43 38.38687389973343 41.0 38.0 41.0 35.0 41.0 44-45 38.17189767877579 41.0 38.0 41.0 34.0 41.0 46-47 37.851654627081174 41.0 38.0 41.0 33.5 41.0 48-49 37.57281931962319 40.0 38.0 41.0 33.0 41.0 50-51 37.422442010395926 40.0 38.0 41.0 33.0 41.0 52-53 37.31601558438321 40.0 38.0 41.0 33.0 41.0 54-55 37.17565642063664 40.0 37.0 41.0 32.5 41.0 56-57 37.01988516062457 40.0 37.0 41.0 32.0 41.0 58-59 36.8441040144088 40.0 36.5 41.0 32.0 41.0 60-61 36.65467579057649 40.0 36.0 41.0 31.0 41.0 62-63 36.435142206840766 39.5 35.5 41.0 31.0 41.0 64-65 36.175507422360994 39.0 35.0 41.0 31.0 41.0 66-67 35.892341816640766 39.0 35.0 41.0 30.5 41.0 68-69 35.62153790165163 38.0 35.0 41.0 30.0 41.0 70-71 35.36206138171341 37.0 35.0 40.0 30.0 41.0 72-73 35.090510671294226 37.0 35.0 39.5 30.0 41.0 74-75 34.77994004315948 36.5 35.0 39.0 30.0 41.0 76-77 34.445375444493756 36.0 35.0 39.0 30.0 41.0 78-79 34.10886501199038 36.0 35.0 38.5 30.0 40.0 80-81 33.78098442512906 35.0 35.0 37.0 29.0 39.0 82-83 33.43989908134404 35.0 34.5 37.0 29.0 39.0 84-85 33.1597015507957 35.0 34.0 36.5 29.0 38.5 86-87 32.918627752942 35.0 34.0 36.0 29.0 37.0 88-89 32.67920113635581 35.0 34.0 36.0 29.0 37.0 90-91 32.470658656020795 35.0 34.0 35.5 29.0 36.5 92-93 32.31571201167189 35.0 34.0 35.0 29.0 36.0 94-95 32.17233499732508 35.0 34.0 35.0 28.5 36.0 96-97 32.03138410459827 35.0 34.0 35.0 27.5 36.0 98-99 31.90688526197948 35.0 34.0 35.0 27.0 36.0 100 31.80150526799517 35.0 34.0 35.0 27.0 36.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.22226111873074217 1101 2 0.1825275165985687 1101 3 0.20616882895580346 1101 4 0.1471440183342949 1101 5 0.1587101309402712 1101 6 0.15712595047231304 1101 7 0.16668273307337955 1101 8 0.16724317277856215 1101 9 0.22021709145420942 1101 10-11 0.18511357188046418 1101 12-13 0.2231092959348615 1101 14-15 0.2853244423863899 1101 16-17 0.3071926691492308 1101 18-19 0.27463904166072695 1101 20-21 0.26618543289038143 1101 22-23 0.26607578593640113 1101 24-25 0.27048875219298196 1101 26-27 0.2797109901559338 1101 28-29 0.28055125818204374 1101 30-31 0.3266036570020461 1101 32-33 0.34394671612167116 1101 34-35 0.3536844719502952 1101 36-37 0.33425362981652285 1101 38-39 0.3319479974220272 1101 40-41 0.3527024088177768 1101 42-43 0.38631761041158086 1101 44-45 0.4217055440332089 1101 46-47 0.47505890392040584 1101 48-49 0.3857006196190369 1101 50-51 0.3744772746588225 1101 52-53 0.3466088000613894 1101 54-55 0.3585754519794975 1101 56-57 0.3028618385601831 1101 58-59 0.33700873481439686 1101 60-61 0.33677089970333896 1101 62-63 0.3483404422137113 1101 64-65 0.39604891715525525 1101 66-67 0.3841154561311484 1101 68-69 0.3537953771219762 1101 70-71 0.3426153763171911 1101 72-73 0.3486868095818707 1101 74-75 0.2840299690158261 1101 76-77 0.3190242609885203 1101 78-79 0.28722151713823507 1101 80-81 0.32141046803779005 1101 82-83 0.3522481683131602 1101 84-85 0.34381344512629397 1101 86-87 0.3201535338860211 1101 88-89 0.34373104145502964 1101 90-91 0.34809056458350085 1101 92-93 0.40061546875109855 1101 94-95 0.382407262591137 1101 96-97 0.38092726528201837 1101 98-99 0.40591562340911835 1101 100 0.4672242220991514 1102 1 0.11851398609587704 1102 2 0.1436614113512178 1102 3 0.15368071454315668 1102 4 0.09189815608713303 1102 5 0.10960480639374737 1102 6 0.09329248744146668 1102 7 0.14574774191314077 1102 8 0.12738542894332738 1102 9 0.1919152871283245 1102 10-11 0.19293760787940073 1102 12-13 0.16359144504387757 1102 14-15 0.2675011582085247 1102 16-17 0.2810456476900356 1102 18-19 0.27931782219800994 1102 20-21 0.2662244133606819 1102 22-23 0.2586172433387546 1102 24-25 0.2797531176168917 1102 26-27 0.2538229936775309 1102 28-29 0.2379520066294134 1102 30-31 0.2612851658231534 1102 32-33 0.33405665235875404 1102 34-35 0.30332222796883457 1102 36-37 0.2791251348818733 1102 38-39 0.2523398718672425 1102 40-41 0.2716907140343352 1102 42-43 0.2950680089437796 1102 44-45 0.3331755687148288 1102 46-47 0.3507505611529993 1102 48-49 0.3737628907353212 1102 50-51 0.3516862531562879 1102 52-53 0.3465196120750704 1102 54-55 0.35888058604606243 1102 56-57 0.3665695907961535 1102 58-59 0.30301918903720093 1102 60-61 0.29078078011799136 1102 62-63 0.2644392005024585 1102 64-65 0.3001478417271599 1102 66-67 0.3772070527377025 1102 68-69 0.3231105904503693 1102 70-71 0.3062294986225069 1102 72-73 0.30251112955245674 1102 74-75 0.3187797282208251 1102 76-77 0.3454329396400837 1102 78-79 0.3296267390885532 1102 80-81 0.29850045241823864 1102 82-83 0.3454414716205747 1102 84-85 0.3437001483160458 1102 86-87 0.32706869584927745 1102 88-89 0.3142801825134143 1102 90-91 0.33434888404779883 1102 92-93 0.3126267096490949 1102 94-95 0.2823279317833851 1102 96-97 0.28483891183942234 1102 98-99 0.26641814206556447 1102 100 0.2882838669804393 1103 1 0.13186281149383206 1103 2 0.16782275359911836 1103 3 0.15491418017123948 1103 4 0.09350709952865088 1103 5 0.09242581067371702 1103 6 0.12181644188778051 1103 7 0.11199877927668211 1103 8 0.13962895543226495 1103 9 0.2160931497408498 1103 10-11 0.20621417213134663 1103 12-13 0.20087667206417592 1103 14-15 0.29454491270321626 1103 16-17 0.27817238138077016 1103 18-19 0.23149221116698726 1103 20-21 0.2644258153093091 1103 22-23 0.31176399789870857 1103 24-25 0.2755567390126572 1103 26-27 0.2827172967966476 1103 28-29 0.28971335764423145 1103 30-31 0.31849436455992475 1103 32-33 0.31114051714521196 1103 34-35 0.2847133967504334 1103 36-37 0.3092782609468472 1103 38-39 0.285861854581249 1103 40-41 0.26802760126476954 1103 42-43 0.2905443952866378 1103 44-45 0.30139112873953167 1103 46-47 0.27209914447200134 1103 48-49 0.25004348678018573 1103 50-51 0.27911523701132523 1103 52-53 0.2695420873513754 1103 54-55 0.2816484375977737 1103 56-57 0.2992668818518567 1103 58-59 0.23058064479361207 1103 60-61 0.1712579813556374 1103 62-63 0.29740394019424343 1103 64-65 0.2762495481778302 1103 66-67 0.25404909399230036 1103 68-69 0.25930700596794765 1103 70-71 0.2496768710852919 1103 72-73 0.256662997927414 1103 74-75 0.21406603561529636 1103 76-77 0.23636314410699555 1103 78-79 0.21829596068522505 1103 80-81 0.23836772591374 1103 82-83 0.24226724216992324 1103 84-85 0.22963776264026592 1103 86-87 0.248918307633069 1103 88-89 0.23064585720017305 1103 90-91 0.2816204943326426 1103 92-93 0.28843232667194485 1103 94-95 0.27522283606249687 1103 96-97 0.26306006571726215 1103 98-99 0.27108796712932204 1103 100 0.29338503423277373 1104 1 0.08348205855538282 1104 2 0.11526109702917609 1104 3 0.11525176690643946 1104 4 0.029989072872581346 1104 5 0.06758767738128313 1104 6 0.09719794845033647 1104 7 0.06388394588775981 1104 8 0.0890180376431573 1104 9 0.12140791383338723 1104 10-11 0.13194934494151056 1104 12-13 0.1425383363527004 1104 14-15 0.1856746326212857 1104 16-17 0.18546825406227185 1104 18-19 0.20402149816930404 1104 20-21 0.17647906182590845 1104 22-23 0.19071206798976448 1104 24-25 0.1810512590002631 1104 26-27 0.20247160365850903 1104 28-29 0.1788912264797915 1104 30-31 0.18531514856813658 1104 32-33 0.18635041939495522 1104 34-35 0.19444872575953553 1104 36-37 0.2415083221150951 1104 38-39 0.22207441257040017 1104 40-41 0.22761337138291537 1104 42-43 0.249710847537294 1104 44-45 0.23016993631004112 1104 46-47 0.2275625656966369 1104 48-49 0.19368391587916278 1104 50-51 0.12554552527184626 1104 52-53 0.16385563176540074 1104 54-55 0.12635098370000009 1104 56-57 0.13104849476633973 1104 58-59 0.14536884249427118 1104 60-61 0.10677151733206358 1104 62-63 0.12142152854386978 1104 64-65 0.16709874958694115 1104 66-67 0.18276092511217712 1104 68-69 0.1672356392626213 1104 70-71 0.12983717123513117 1104 72-73 0.12381969704032514 1104 74-75 0.136087133832838 1104 76-77 0.06363560522181899 1104 78-79 0.060337483939463255 1104 80-81 0.07558932822640685 1104 82-83 0.092316225154768 1104 84-85 0.10106701109735639 1104 86-87 0.109071498586232 1104 88-89 0.11804988294566243 1104 90-91 0.0884486322439102 1104 92-93 0.14649866092725716 1104 94-95 0.12987410624943152 1104 96-97 0.12931748879157823 1104 98-99 0.15598859906365803 1104 100 0.13654545351806746 1105 1 -0.05286766778760921 1105 2 5.432024425999771E-5 1105 3 0.018041899641048076 1105 4 -0.09489750812043951 1105 5 -0.10072445816761899 1105 6 -0.1242892771382671 1105 7 -0.495773314424369 1105 8 -0.40565092178140816 1105 9 -0.3756853749782181 1105 10-11 -0.3692585118729639 1105 12-13 -0.252981485547501 1105 14-15 -0.2433102229292885 1105 16-17 -0.17563805597803395 1105 18-19 -0.19793948863131305 1105 20-21 -0.19075605162369413 1105 22-23 -0.18755407430737137 1105 24-25 -0.21303830679733693 1105 26-27 -0.2580629703117765 1105 28-29 -0.2635468937566543 1105 30-31 -0.24628215379773621 1105 32-33 -0.2652178679964692 1105 34-35 -0.25464418186516014 1105 36-37 -0.23809729276639757 1105 38-39 -0.27309051659175765 1105 40-41 -0.2674571870458351 1105 42-43 -0.2556070077074253 1105 44-45 -0.24711286616577866 1105 46-47 -0.2105119347635238 1105 48-49 -0.18271015101994692 1105 50-51 -0.1722935016885856 1105 52-53 -0.2388625956237007 1105 54-55 -0.20827354579003554 1105 56-57 -0.20091263818007832 1105 58-59 -0.20517605790495708 1105 60-61 -0.22962556347194862 1105 62-63 -0.2100719982236967 1105 64-65 -0.1849044483892115 1105 66-67 -0.1797889068825569 1105 68-69 -0.21902876490597833 1105 70-71 -0.17108035868822213 1105 72-73 -0.18101240659484574 1105 74-75 -0.21918638850525696 1105 76-77 -0.18437959990026087 1105 78-79 -0.13924019092429774 1105 80-81 -0.15829903304794612 1105 82-83 -0.13394137256493366 1105 84-85 -0.1504056896919863 1105 86-87 -0.14439754179426245 1105 88-89 -0.1092823695529006 1105 90-91 -0.11194339280105225 1105 92-93 -0.1104651161309107 1105 94-95 -0.17916351397511932 1105 96-97 -0.2040050547138783 1105 98-99 -0.2144796983888675 1105 100 -0.15598425343463518 1106 1 0.11174168796335238 1106 2 0.11502697423778585 1106 3 0.100822893536062 1106 4 0.08662169204421133 1106 5 0.09705283368705864 1106 6 0.11868601603515572 1106 7 0.13855061723712225 1106 8 0.122309313260196 1106 9 0.12856409114903045 1106 10-11 0.13577629348084486 1106 12-13 0.11549378825718293 1106 14-15 0.16194022276454945 1106 16-17 0.1963125695763921 1106 18-19 0.19414723926598043 1106 20-21 0.16013265340720295 1106 22-23 0.18934665250509397 1106 24-25 0.20823008107129226 1106 26-27 0.19720416400738117 1106 28-29 0.21861006840864405 1106 30-31 0.22142523869218422 1106 32-33 0.21784389196152887 1106 34-35 0.2422019341191728 1106 36-37 0.3045394419350558 1106 38-39 0.28726271435240136 1106 40-41 0.3189238452639529 1106 42-43 0.3540945410257237 1106 44-45 0.3957833308896639 1106 46-47 0.4495860353632324 1106 48-49 0.43352636888604934 1106 50-51 0.4330371911943445 1106 52-53 0.37119421608796443 1106 54-55 0.36273801283152096 1106 56-57 0.33417307005370844 1106 58-59 0.33297773126803776 1106 60-61 0.33578825758114306 1106 62-63 0.3539439829121491 1106 64-65 0.37448045986667466 1106 66-67 0.3906020643291228 1106 68-69 0.35873340649896335 1106 70-71 0.3446752637827686 1106 72-73 0.3525894553655817 1106 74-75 0.33554938674284784 1106 76-77 0.29445556289289243 1106 78-79 0.31173804101244684 1106 80-81 0.32498667757690924 1106 82-83 0.3643398978603045 1106 84-85 0.3343403375849263 1106 86-87 0.32122409333172186 1106 88-89 0.34560360268781665 1106 90-91 0.3288450583830951 1106 92-93 0.3311129876202159 1106 94-95 0.3560171322151149 1106 96-97 0.3881439174171035 1106 98-99 0.34927668190870875 1106 100 0.3733461881946276 1107 1 -0.014396081653565318 1107 2 0.041892106861581624 1107 3 0.033867375787806964 1107 4 -0.05130885079918812 1107 5 -0.026927321036325225 1107 6 -0.0024300349139494415 1107 7 0.009094481162698287 1107 8 0.019399656584390357 1107 9 0.04218440870415918 1107 10-11 0.034165972576602144 1107 12-13 0.0718227861031906 1107 14-15 0.07134143869879495 1107 16-17 0.0919914502148842 1107 18-19 0.07161750955018675 1107 20-21 0.087661581433089 1107 22-23 0.08023424732579798 1107 24-25 0.08988685623408088 1107 26-27 0.06940143352198191 1107 28-29 0.11249347118427266 1107 30-31 0.10192064608481388 1107 32-33 0.11306455262931792 1107 34-35 0.10118358204018563 1107 36-37 0.1315121573763136 1107 38-39 0.07633809311484896 1107 40-41 0.054353991841551874 1107 42-43 0.03861734998842081 1107 44-45 0.05307047057257108 1107 46-47 0.0679049454955063 1107 48-49 0.036628225361539535 1107 50-51 0.09422198203603926 1107 52-53 0.09037343373471174 1107 54-55 0.055865479853999034 1107 56-57 0.07777169681163798 1107 58-59 0.03801745898585551 1107 60-61 0.03894393203572122 1107 62-63 0.09744010461778174 1107 64-65 0.08703114183676774 1107 66-67 0.10623441608593964 1107 68-69 0.04008832203398782 1107 70-71 0.06790187069333342 1107 72-73 0.04779846125641285 1107 74-75 0.022910961533412433 1107 76-77 0.03725365865972918 1107 78-79 0.03714981684395724 1107 80-81 0.06585548828459764 1107 82-83 0.05508970219222675 1107 84-85 0.048333620439926506 1107 86-87 0.026772976963400197 1107 88-89 0.07197338693910638 1107 90-91 0.11782978699588398 1107 92-93 0.08547086848643914 1107 94-95 0.04266809123942039 1107 96-97 0.05158463347990505 1107 98-99 0.045669250299205544 1107 100 0.07934235272376355 1108 1 -0.03031854309512738 1108 2 -0.023936215432698305 1108 3 -0.025770739248947905 1108 4 0.022038490479637574 1108 5 -0.16454043374515237 1108 6 -0.14978164746795386 1108 7 -0.13734129035057663 1108 8 -0.1245641579474821 1108 9 -0.1368794537489677 1108 10-11 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-0.06456903664007996 2214 62-63 -0.068837368851419 2214 64-65 -0.013633544987364132 2214 66-67 -0.01713027068139894 2214 68-69 0.009277022619635034 2214 70-71 -0.07881886011350048 2214 72-73 -0.025470690002158847 2214 74-75 -0.02572617196604199 2214 76-77 -0.02739405718150323 2214 78-79 -0.10418047137976316 2214 80-81 -0.06126565639146264 2214 82-83 -0.014396465629957333 2214 84-85 -0.07195388707200578 2214 86-87 -0.06884458802905868 2214 88-89 -0.06339911361686745 2214 90-91 -0.06368549414204949 2214 92-93 -0.1386560020120342 2214 94-95 -0.12542433986479296 2214 96-97 -0.10894225163067617 2214 98-99 -0.12731787339528822 2214 100 -0.1484514754801438 2215 1 -0.15991434964551132 2215 2 -0.17376648124407978 2215 3 -0.14792551879934734 2215 4 -0.11913199260129659 2215 5 -0.07293930798326187 2215 6 -0.13933471024173372 2215 7 -0.1730796044690237 2215 8 -0.16533258997758793 2215 9 -0.19630512825922608 2215 10-11 -0.20826841079280456 2215 12-13 -0.17879733635178496 2215 14-15 -0.2942702326800557 2215 16-17 -0.26088705153864566 2215 18-19 -0.2854412810028677 2215 20-21 -0.25995917795639656 2215 22-23 -0.26237153363071997 2215 24-25 -0.2545544125997665 2215 26-27 -0.29779954148698096 2215 28-29 -0.3385496447616134 2215 30-31 -0.3217243046462457 2215 32-33 -0.3709679051011179 2215 34-35 -0.34472943754386165 2215 36-37 -0.3073874818720981 2215 38-39 -0.37655343135745767 2215 40-41 -0.32546926486747907 2215 42-43 -0.34261803601157936 2215 44-45 -0.34912411713094826 2215 46-47 -0.3554758851841697 2215 48-49 -0.34962291931572054 2215 50-51 -0.38851924991728026 2215 52-53 -0.3907647691591478 2215 54-55 -0.3849746809112631 2215 56-57 -0.45174910580632144 2215 58-59 -0.3753994117171473 2215 60-61 -0.36241448896811335 2215 62-63 -0.3922955070053149 2215 64-65 -0.3302403221093755 2215 66-67 -0.32527738445622134 2215 68-69 -0.35075252377593813 2215 70-71 -0.4050024633291045 2215 72-73 -0.37513124101041484 2215 74-75 -0.3505274384158028 2215 76-77 -0.3382835886027138 2215 78-79 -0.4699007649503457 2215 80-81 -0.4157842644688401 2215 82-83 -0.38500915289399273 2215 84-85 -0.3661804749233539 2215 86-87 -0.36081273385278223 2215 88-89 -0.3247450488868253 2215 90-91 -0.3862006065713075 2215 92-93 -0.4315407289664748 2215 94-95 -0.4353036644277921 2215 96-97 -0.4463475670407959 2215 98-99 -0.4915370812094544 2215 100 -0.44799368738004475 2216 1 -0.1778238981847977 2216 2 -0.18169980388499596 2216 3 -0.2911783044366274 2216 4 -0.1501894276302238 2216 5 -0.21767954551673796 2216 6 -0.17142959483456366 2216 7 -0.27831941402594396 2216 8 -0.26727219591563767 2216 9 -0.24366051697166569 2216 10-11 -0.25528522317294033 2216 12-13 -0.27911429639616614 2216 14-15 -0.34491103518735144 2216 16-17 -0.4292474137522433 2216 18-19 -0.4163648089906289 2216 20-21 -0.3726163449525046 2216 22-23 -0.40687718141050766 2216 24-25 -0.4671792683449354 2216 26-27 -0.5108881593147174 2216 28-29 -0.4582670261036128 2216 30-31 -0.48107849063951136 2216 32-33 -0.47925432938559 2216 34-35 -0.4991481079328395 2216 36-37 -0.5082871355485921 2216 38-39 -0.5212184480749116 2216 40-41 -0.52684073046197 2216 42-43 -0.5175018678573551 2216 44-45 -0.4455514679130701 2216 46-47 -0.4318465814026027 2216 48-49 -0.4308300533062308 2216 50-51 -0.37194854288433987 2216 52-53 -0.35894023441038314 2216 54-55 -0.4465966670490573 2216 56-57 -0.45359591354679907 2216 58-59 -0.5461657463846947 2216 60-61 -0.4873629280554326 2216 62-63 -0.5060578286582214 2216 64-65 -0.518495731764105 2216 66-67 -0.47177951859094236 2216 68-69 -0.4670304380745165 2216 70-71 -0.46676385277800847 2216 72-73 -0.4978260059519144 2216 74-75 -0.41051173449673684 2216 76-77 -0.4280206613936244 2216 78-79 -0.37488355966702613 2216 80-81 -0.4340311176444729 2216 82-83 -0.41114712112163687 2216 84-85 -0.4163579222907856 2216 86-87 -0.393976758672828 2216 88-89 -0.3406798570720895 2216 90-91 -0.3027413753379733 2216 92-93 -0.43092023035687177 2216 94-95 -0.43583275353181605 2216 96-97 -0.5210660758684256 2216 98-99 -0.5013632088337054 2216 100 -0.4912708743194045 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 78.0 3 559.0 4 279.0 5 538.0 6 708.0 7 1150.0 8 2113.0 9 3307.0 10 4538.0 11 6828.0 12 10965.0 13 16544.0 14 17753.0 15 22194.0 16 29149.0 17 45539.0 18 56400.0 19 28591.0 20 17131.0 21 14878.0 22 14647.0 23 15036.0 24 17261.0 25 20673.0 26 25393.0 27 32276.0 28 39532.0 29 49284.0 30 61722.0 31 76550.0 32 95820.0 33 122889.0 34 167970.0 35 265135.0 36 451186.0 37 1032421.0 38 2052084.0 39 1267160.0 40 11105.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.38778864246518 21.872318272357244 14.794017073830013 23.94587601134756 2 12.943794757916713 22.56821571870564 41.515895748751305 22.972093774626337 3 14.650652085492737 29.612406024902583 32.50129564270438 23.2356462469003 4 9.93874084396948 19.09660006845753 41.41883887567611 29.545820211896885 5 10.848698114241087 39.53018555820478 36.63422325567055 12.98689307188359 6 26.515788897078192 41.49704479919756 19.153765236447224 12.833401067277025 7 23.44060225152221 34.94354794005169 25.04886520223584 16.56698460619026 8 24.01507793667647 35.687079676438394 22.14698232980494 18.150860057080198 9 25.58009284634432 15.74266415149049 22.6592182289263 36.01802477323889 10-11 22.275840827528388 27.498021283218744 30.748865169434904 19.477272719817968 12-13 23.906949633826695 26.092295944524423 30.36256028402991 19.63819413761897 14-15 21.259774270482463 26.87622204006766 29.11048275441312 22.753520935036754 16-17 19.418395030263788 30.174020473691517 29.203539024755855 21.204045471288843 18-19 19.342370648668133 28.3040552131684 32.92865992082509 19.424914217338383 20-21 20.815985735526667 27.449377487336374 32.567841366775866 19.166795410361097 22-23 20.279974372597277 27.579101809228046 32.58016825096748 19.56075556720719 24-25 20.009530327549044 27.837289706705032 32.376134852740776 19.777045113005148 26-27 19.81754523729747 27.76856885461825 32.46562808382072 19.948257824263568 28-29 19.88941853914387 27.597409679506473 32.67666997686687 19.836501804482793 30-31 20.2254400901124 27.86268092921525 32.32049273166992 19.591386249002422 32-33 19.68469746214448 27.639483810039746 32.71067630305671 19.965142424759065 34-35 20.044032921608213 27.606027460259742 32.44297345477609 19.906966163355953 36-37 20.42000157451387 27.81911787860498 31.799751358018213 19.961129188862937 38-39 20.196678384042023 27.86271366934368 31.953082498512025 19.98752544810227 40-41 20.037617563085192 27.789876322584707 32.04665063295125 20.125855481378846 42-43 20.090458940208798 27.775918136266363 31.866144516504868 20.267478407019965 44-45 20.278199271946725 27.83613913965332 31.523253685695895 20.362407902704057 46-47 20.595102044297377 28.099313844028266 30.930338669373064 20.375245442301296 48-49 20.685569939219157 28.057208240425762 30.604355237502386 20.6528665828527 50-51 20.80074452467833 28.23102569935314 30.074419666498997 20.89381010946953 52-53 20.727590598264666 28.34633207514369 30.08248618055117 20.843591146040474 54-55 20.848153984322366 28.69218716071315 29.908461842755248 20.551197012209244 56-57 20.808031639393963 28.307028537301253 29.98846791356651 20.896471909738278 58-59 20.715433815311624 27.89734958819307 30.278455236717743 21.10876135977756 60-61 20.90542598586077 28.29673717912763 29.991888295298853 20.805948539712748 62-63 20.567690810455495 28.200461640447234 30.277851853236783 20.95399569586049 64-65 20.597318260644805 28.06157425493482 30.3290541225371 21.01205336188327 66-67 20.64013291068915 28.04854257869534 30.08198263911525 21.22934187150026 68-69 20.99881128453105 28.245760690976944 29.851182598045245 20.904245426446757 70-71 20.975140850799182 28.087958601542475 29.919880037367776 21.01702051029057 72-73 20.80745303415031 28.089971393462687 29.94951522231056 21.153060350076448 74-75 20.79245740075278 28.108315511808048 29.90618821252787 21.193038874911302 76-77 20.850413461490362 28.23015226171115 29.745356333110205 21.174077943688285 78-79 20.967485873709414 28.327786359873908 29.707056494829942 20.99767127158674 80-81 20.79507824481409 28.285635187951723 29.82773463234852 21.091551934885672 82-83 20.682613335979312 28.266713025965174 29.921510372824706 21.12916326523081 84-85 20.7311448942928 28.098183888840232 29.717700285732374 21.452970931134594 86-87 20.795205211034038 28.240563410172204 29.660913612009242 21.303317766784513 88-89 20.55604908118539 28.068449476410446 29.848421892004236 21.52707955039993 90-91 20.45664252031731 28.02860430514408 29.808943814799555 21.705809359739057 92-93 20.528240020803086 28.131240854878364 29.857524919546712 21.482994204771835 94-95 20.448797213267238 27.823382061576403 29.936687752708384 21.791132972447976 96-97 20.356994839132714 28.122011450771673 29.696340847181286 21.824652862914327 98-99 20.13887288663271 27.942432223941672 29.870192704704206 22.048502184721407 100 20.27917537494028 28.011171082457746 29.67622197245247 22.0334315701495 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3837.0 1 2378.0 2 1973.0 3 2751.5 4 1663.0 5 1247.5 6 2892.5 7 8341.5 8 14538.0 9 14468.0 10 8975.5 11 5061.0 12 4695.0 13 4982.5 14 5468.5 15 6215.5 16 6986.0 17 7986.5 18 9342.5 19 10878.0 20 12963.5 21 15885.0 22 20452.5 23 27070.5 24 35380.5 25 45188.0 26 58073.0 27 74378.0 28 93566.0 29 114253.0 30 132361.0 31 149037.0 32 165752.0 33 179136.5 34 190850.5 35 203146.0 36 212965.5 37 220876.5 38 229685.5 39 233601.5 40 233544.0 41 233283.5 42 228614.5 43 224117.0 44 219470.5 45 209686.5 46 200035.0 47 192254.5 48 179395.0 49 171085.5 50 167266.5 51 156804.0 52 143197.0 53 131987.0 54 122850.0 55 113263.0 56 101198.5 57 88798.5 58 80499.5 59 72860.0 60 63194.5 61 51980.0 62 42063.5 63 33385.0 64 25713.5 65 19765.5 66 15036.5 67 11134.0 68 8082.5 69 5761.0 70 4146.5 71 3103.0 72 2172.5 73 1365.0 74 975.0 75 719.5 76 435.0 77 284.5 78 190.5 79 97.5 80 48.0 81 21.0 82 11.0 83 7.0 84 4.0 85 6.5 86 7.5 87 3.0 88 1.5 89 3.0 90 3.5 91 2.5 92 2.0 93 2.0 94 6.5 95 13.0 96 17.0 97 16.5 98 8.5 99 2.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.009348268257905929 2 1.640047062790514E-5 3 4.2641223632553356E-4 4 0.002870082359883399 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.0660305908138339E-4 24-25 1.4760423565114624E-4 26-27 6.396183544883004E-4 28-29 0.0011644334145812648 30-31 0.0018368527103253757 32-33 0.004747936246778538 34-35 0.0058549680141621345 36-37 0.004362525187022767 38-39 0.002837281418627589 40-41 0.0038377101269298024 42-43 5.576160013487746E-4 44-45 4.920141188371541E-4 46-47 0.003772108244418182 48-49 0.0025010717707555337 50-51 0.001197234355837075 52-53 1.640047062790514E-5 54-55 6.232178838603953E-4 56-57 8.282237667092095E-4 58-59 8.282237667092095E-4 60-61 3.9361129506972336E-4 62-63 0.0 64-65 0.0 66-67 9.594275317324506E-4 68-69 7.872225901394467E-4 70-71 0.0025256724766973913 72-73 5.740164719766799E-5 74-75 0.0020418585931741896 76-77 3.690105891278656E-4 78-79 0.0010988315320696442 80-81 8.036230607673518E-4 82-83 0.001369439297430079 84-85 0.00118083388520917 86-87 2.7060776536043477E-4 88-89 0.0016482472981044663 90-91 0.0020008574166044267 92-93 0.0035179009496856526 94-95 0.0010906312967556917 96-97 0.0025420729473252966 98-99 0.002550273182639249 100 0.003526101184999605 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6097386.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.12344037459148 #Duplication Level Percentage of deduplicated Percentage of total 1 71.49442595016811 39.410187259797375 2 16.88496448365199 18.615146658833705 3 5.6672602893432735 9.37196653940712 4 2.391954951970355 5.274111446945868 5 1.219887159385577 3.362218854706031 6 0.6510474294587424 2.1532784495280026 7 0.4051810821067399 1.5634482654296333 8 0.2534807950675945 1.1178186794410072 9 0.17081373828870702 0.8474256825946755 >10 0.7642147869168382 7.314650845468342 >50 0.051919852953098894 1.999713372250236 >100 0.038674234124041036 4.199340933006908 >500 0.004116854293118053 1.5636764740731606 >1k 0.0019688969556253453 1.8665923882987823 >5k 0.0 0.0 >10k+ 8.949531611126874E-5 1.3404241502191843 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 36213 0.5939102428483288 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24590 0.40328757274018734 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20710 0.3396537467039154 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 1.640047062790514E-5 0.0 1.640047062790514E-5 0.0 4 0.0 1.640047062790514E-5 0.0 8.20023531395257E-5 0.0 5 0.0 1.640047062790514E-5 0.0 9.840282376743083E-5 0.0 6 0.0 1.640047062790514E-5 0.0 9.840282376743083E-5 0.0 7 0.0 1.640047062790514E-5 0.0 9.840282376743083E-5 0.0 8 0.0 1.640047062790514E-5 0.0 9.840282376743083E-5 0.0 9 0.0 1.640047062790514E-5 0.0 1.312037650232411E-4 0.0 10-11 0.0 1.640047062790514E-5 0.0 1.312037650232411E-4 0.0 12-13 0.0 1.640047062790514E-5 0.0 1.4760423565114624E-4 0.0 14-15 0.0 1.640047062790514E-5 0.0 1.640047062790514E-4 0.0 16-17 0.0 1.640047062790514E-5 0.0 2.0500588284881423E-4 0.0 18-19 0.0 1.640047062790514E-5 0.0 2.3780682410462451E-4 0.0 20-21 0.0 1.640047062790514E-5 0.0 2.4600705941857706E-4 0.0 22-23 0.0 1.640047062790514E-5 0.0 4.428127069534387E-4 0.0 24-25 0.0 1.640047062790514E-5 0.0 8.036230607673517E-4 0.0 26-27 0.0 1.640047062790514E-5 0.0 0.0015088432977672728 0.0 28-29 0.0 1.640047062790514E-5 0.0 0.0037803084797321347 0.0 30-31 0.0 1.640047062790514E-5 0.0 0.009012058610033874 0.0 32-33 0.0 1.640047062790514E-5 0.0 0.019032746163683913 0.0 34-35 0.0 1.640047062790514E-5 0.0 0.03234172807822894 0.0 36-37 0.0 1.640047062790514E-5 0.0 0.053121124363784744 0.0 38-39 0.0 1.640047062790514E-5 0.0 0.08204335431609545 0.0 40-41 0.0 1.640047062790514E-5 0.0 0.11448348521809182 0.0 42-43 0.0 1.640047062790514E-5 0.0 0.14532457023386744 0.0 44-45 0.0 1.640047062790514E-5 0.0 0.1837262722091073 0.0 46-47 0.0 1.640047062790514E-5 0.0 0.22891776902429992 0.0 48-49 0.0 1.640047062790514E-5 0.0 0.27827498537898043 0.0 50-51 0.0 3.280094125581028E-5 0.0 0.33178152080252094 0.0 52-53 0.0 3.280094125581028E-5 0.0 0.384509033871236 0.0 54-55 0.0 3.280094125581028E-5 0.0 0.4416236728329156 0.0 56-57 0.0 3.280094125581028E-5 0.0 0.5076437673455477 0.0 58-59 0.0 3.280094125581028E-5 0.0 0.584340568236946 0.0 60-61 0.0 3.280094125581028E-5 0.0 0.668573385381867 0.0 62-63 0.0 3.280094125581028E-5 0.0 0.7559632931226594 0.0 64-65 0.0 3.280094125581028E-5 0.0 0.846969504636905 0.0 66-67 0.0 3.280094125581028E-5 0.0 0.9496364507675912 0.0 68-69 0.0 3.280094125581028E-5 0.0 1.0680150477598105 0.0 70-71 0.0 3.280094125581028E-5 0.0 1.2035485370288186 0.0 72-73 0.0 3.280094125581028E-5 0.0 1.3501851449129185 0.0 74-75 0.0 3.280094125581028E-5 0.0 1.4997574370394133 0.0 76-77 0.0 3.280094125581028E-5 0.0 1.6576611682448839 0.0 78-79 0.0 3.280094125581028E-5 0.0 1.837771136680538 0.0 80-81 0.0 3.280094125581028E-5 0.0 2.046467125420631 0.0 82-83 0.0 3.280094125581028E-5 0.0 2.274417266677885 0.0 84-85 0.0 3.280094125581028E-5 0.0 2.505204689353766 0.0 86-87 0.0 3.280094125581028E-5 0.0 2.7510805450073192 0.0 88 0.0 3.280094125581028E-5 0.0 2.9458853351255767 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11650 0.0 63.989742 1 GTATCAA 25300 0.0 49.38187 1 ATCAACG 34250 0.0 36.117332 3 TCAACGC 34525 0.0 35.816036 4 CAACGCA 35630 0.0 34.718174 5 TATCAAC 36495 0.0 33.985714 2 AACGCAG 36745 0.0 33.70305 6 ACGCAGA 40800 0.0 30.157564 7 CGCAGAG 41100 0.0 29.857391 8 GTACATG 25890 0.0 27.886389 1 TACATGG 24865 0.0 27.74776 2 GCAGAGT 44640 0.0 27.173813 9 ACATGGG 25430 0.0 26.33655 3 AGAGTAC 42435 0.0 23.762444 10-11 GTGGTAT 3870 0.0 23.441177 1 CATGGGG 13395 0.0 23.192581 4 AGTACTT 32275 0.0 22.716696 12-13 GAGTACT 30600 0.0 22.67769 12-13 CATGGGT 6980 0.0 22.018244 4 CAGAGTA 44300 0.0 21.510174 10-11 >>END_MODULE