##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431368_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3204563
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.929609435046213	33.0	31.0	34.0	26.0	34.0
2	30.985119655940608	34.0	31.0	34.0	27.0	34.0
3	31.092799860698634	34.0	31.0	34.0	27.0	34.0
4	34.20966540523622	37.0	35.0	37.0	30.0	37.0
5	34.45163786762813	37.0	35.0	37.0	31.0	37.0
6	34.435881273047215	37.0	35.0	37.0	31.0	37.0
7	34.36524262434535	37.0	35.0	37.0	31.0	37.0
8	34.28971500950364	37.0	35.0	37.0	31.0	37.0
9	35.67459712915615	39.0	37.0	39.0	30.0	39.0
10-11	35.74910463610795	39.0	37.0	39.0	30.0	39.0
12-13	35.719947150360284	39.0	37.0	39.0	30.0	39.0
14-15	37.07078453442794	41.0	38.0	41.0	30.0	41.0
16-17	37.09728455954837	41.0	38.0	41.0	30.0	41.0
18-19	37.1149169169088	41.0	38.0	41.0	30.0	41.0
20-21	37.16386898931305	41.0	38.0	41.0	30.0	41.0
22-23	37.20060597966088	41.0	38.0	41.0	30.0	41.0
24-25	37.2533153506422	41.0	38.0	41.0	30.0	41.0
26-27	37.27404469813825	41.0	38.0	41.0	30.0	41.0
28-29	37.26613691164755	40.0	38.0	41.0	30.0	41.0
30-31	37.19605481308996	40.0	38.0	41.0	30.0	41.0
32-33	37.12394732136644	40.0	38.0	41.0	30.0	41.0
34-35	37.06570521472038	40.0	38.0	41.0	30.0	41.0
36-37	36.967360604238394	40.0	38.0	41.0	30.0	41.0
38-39	36.857488056873905	40.0	38.0	41.0	29.5	41.0
40-41	36.76954314831694	40.0	37.0	41.0	29.0	41.0
42-43	36.64628952528005	40.0	37.0	41.0	29.0	41.0
44-45	36.513364068673326	40.0	37.0	41.0	28.5	41.0
46-47	36.38234136760613	40.0	36.5	41.0	28.0	41.0
48-49	36.23785864094418	40.0	36.0	41.0	28.0	41.0
50-51	36.104031345303554	40.0	36.0	41.0	28.0	41.0
52-53	35.94147080272724	40.0	35.0	41.0	28.0	41.0
54-55	35.78836755588827	40.0	35.0	41.0	28.0	41.0
56-57	35.583678180145	39.0	35.0	41.0	28.0	41.0
58-59	35.35535937349336	39.0	35.0	41.0	27.5	41.0
60-61	35.16689139829674	39.0	35.0	41.0	27.0	41.0
62-63	35.00467520844496	38.5	35.0	41.0	27.0	41.0
64-65	34.75648208507681	38.0	34.0	40.0	26.5	41.0
66-67	34.44400453353546	37.5	34.0	40.0	26.0	41.0
68-69	34.129235561915934	37.0	34.0	40.0	26.0	41.0
70-71	33.87679100083225	36.5	34.0	39.5	26.0	41.0
72-73	33.618093793131855	36.0	34.0	39.0	26.0	41.0
74-75	33.27979134752539	35.5	34.0	39.0	25.5	41.0
76-77	32.93497865387574	35.0	33.0	38.5	25.0	40.0
78-79	32.59972467384789	35.0	33.0	37.0	25.0	39.0
80-81	32.26294458870055	35.0	33.0	37.0	24.5	39.0
82-83	31.927726495001036	35.0	33.0	36.0	24.0	39.0
84-85	31.64254970178461	35.0	33.0	36.0	23.0	37.0
86-87	31.387342361501396	35.0	32.5	36.0	22.5	37.0
88-89	31.14336681787813	35.0	32.0	35.0	20.5	37.0
90-91	30.943446735171065	35.0	32.0	35.0	20.0	36.0
92-93	30.75371509313438	35.0	32.0	35.0	19.0	36.0
94-95	30.550928316903118	35.0	32.0	35.0	16.5	36.0
96-97	30.37176707089235	35.0	32.0	35.0	8.0	36.0
98-99	30.250377976653915	35.0	32.0	35.0	2.0	35.0
100	30.20767605442614	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.28318353517471095
1101	2	0.3234325984444162
1101	3	0.33566922996283566
1101	4	0.39358247138775937
1101	5	0.32920429270426155
1101	6	0.30322009003580064
1101	7	0.3651573515937727
1101	8	0.33837029594259604
1101	9	0.4429751276188014
1101	10-11	0.44187349909435625
1101	12-13	0.41237998228998407
1101	14-15	0.44641842438814194
1101	16-17	0.441176441300108
1101	18-19	0.4244359186476814
1101	20-21	0.4183771943309438
1101	22-23	0.37549285963130785
1101	24-25	0.37661577294625914
1101	26-27	0.36608901905736246
1101	28-29	0.42953309142929896
1101	30-31	0.45783111226579365
1101	32-33	0.4299932161673965
1101	34-35	0.48661929471860077
1101	36-37	0.45406079670851796
1101	38-39	0.4284373609022225
1101	40-41	0.4275441948445362
1101	42-43	0.434938153282566
1101	44-45	0.4592223845707153
1101	46-47	0.4837008550518931
1101	48-49	0.45205601094735925
1101	50-51	0.44069692155496654
1101	52-53	0.43998770869794157
1101	54-55	0.4057252263557345
1101	56-57	0.46178259007105993
1101	58-59	0.5056655563063686
1101	60-61	0.5067641988714513
1101	62-63	0.4903510224646439
1101	64-65	0.4968908523395257
1101	66-67	0.4376652930406948
1101	68-69	0.4750319374953449
1101	70-71	0.5286608226323892
1101	72-73	0.5826566475436223
1101	74-75	0.5348313442169825
1101	76-77	0.5146445384880565
1101	78-79	0.5055956903911962
1101	80-81	0.489877361019353
1101	82-83	0.5211001895477558
1101	84-85	0.5345048897449765
1101	86-87	0.4992235088964314
1101	88-89	0.5315079343981566
1101	90-91	0.5352089685048149
1101	92-93	0.4857430902534752
1101	94-95	0.5346404839455587
1101	96-97	0.5392073557710582
1101	98-99	0.5308145791929384
1101	100	0.57659285422735
1102	1	0.16174219033399595
1102	2	0.23346904508074218
1102	3	0.23610663616848626
1102	4	0.1730269119513963
1102	5	0.24969200586265572
1102	6	0.16233690089399744
1102	7	0.23293394960501246
1102	8	0.2892021186702536
1102	9	0.21944871035633184
1102	10-11	0.24539761988692987
1102	12-13	0.23713386125758262
1102	14-15	0.3383201081167968
1102	16-17	0.32055428714730994
1102	18-19	0.25197503140115174
1102	20-21	0.1660356827705911
1102	22-23	0.14042384328882207
1102	24-25	0.2216146057923538
1102	26-27	0.27556888463382734
1102	28-29	0.3833584019620915
1102	30-31	0.3500835970520413
1102	32-33	0.34774728607089145
1102	34-35	0.3628685277733368
1102	36-37	0.3782836934156819
1102	38-39	0.3003741640298472
1102	40-41	0.2917971377605184
1102	42-43	0.29370401755276276
1102	44-45	0.29596964306881546
1102	46-47	0.2726723712575847
1102	48-49	0.2107222014385144
1102	50-51	0.1907456742429119
1102	52-53	0.18923073226105203
1102	54-55	0.13943789926042172
1102	56-57	0.17802651999758723
1102	58-59	0.11599325295578922
1102	60-61	0.10202527751957291
1102	62-63	0.15402391026534445
1102	64-65	0.19346925772205026
1102	66-67	0.20905266065167183
1102	68-69	0.1519959722736175
1102	70-71	0.14528998611193344
1102	72-73	0.18102974851727538
1102	74-75	0.15663404107014856
1102	76-77	0.14969144876098994
1102	78-79	0.1800953131763663
1102	80-81	0.14308042717049574
1102	82-83	0.17154356250028613
1102	84-85	0.1753867691130786
1102	86-87	0.19636242465993803
1102	88-89	0.130788563019685
1102	90-91	0.14660864216741842
1102	92-93	0.23196104591210798
1102	94-95	0.193555545993906
1102	96-97	0.21185452194466947
1102	98-99	0.22157693443383408
1102	100	0.11743610982415831
1103	1	0.2505292758488302
1103	2	0.2637919072098853
1103	3	0.2771188525733699
1103	4	0.19069706901945693
1103	5	0.25034645612443285
1103	6	0.2519965867578762
1103	7	0.19672103511984318
1103	8	0.3153801291414595
1103	9	0.3400857086111344
1103	10-11	0.3997388066234038
1103	12-13	0.26898377399754736
1103	14-15	0.2750565828113878
1103	16-17	0.26819826620489806
1103	18-19	0.2638642111568217
1103	20-21	0.2446787892278337
1103	22-23	0.27404077173559926
1103	24-25	0.2737524766475019
1103	26-27	0.29073031569840424
1103	28-29	0.3312205311069434
1103	30-31	0.30296317820387486
1103	32-33	0.2708493803117378
1103	34-35	0.2509575330089362
1103	36-37	0.2949503600823533
1103	38-39	0.23700156368080627
1103	40-41	0.21555368226314187
1103	42-43	0.24385672261383462
1103	44-45	0.16835184202169273
1103	46-47	0.08898999778462979
1103	48-49	0.12837054349785149
1103	50-51	0.0884332833179613
1103	52-53	0.09357191899752593
1103	54-55	0.0306900807612962
1103	56-57	-0.0072919791298105
1103	58-59	-0.0013714939901063872
1103	60-61	-0.020575071520568144
1103	62-63	-0.041656718006898075
1103	64-65	-0.04038763582070004
1103	66-67	-0.024149782629301342
1103	68-69	0.06615391293679806
1103	70-71	0.05170359867737773
1103	72-73	0.07151840471274085
1103	74-75	0.05933910215217253
1103	76-77	0.03723507877844412
1103	78-79	0.006338768673742834
1103	80-81	0.024297704657406882
1103	82-83	0.015893475240250865
1103	84-85	-0.002078326872961611
1103	86-87	0.028605007556969042
1103	88-89	0.06010793474743181
1103	90-91	0.01539136468050728
1103	92-93	0.07772893421925886
1103	94-95	0.0743365233062967
1103	96-97	0.05642258477189088
1103	98-99	-0.00802603240735067
1103	100	-0.07562671740097215
1104	1	0.06841802371140204
1104	2	0.07025862499859414
1104	3	0.13630076771078237
1104	4	0.03979119527327413
1104	5	0.04247806893667416
1104	6	0.007187016359296194
1104	7	0.0619780996570114
1104	8	0.07340100103667879
1104	9	0.08272149051393285
1104	10-11	0.020476616660957347
1104	12-13	0.052876915711372874
1104	14-15	0.10401865113863806
1104	16-17	0.16587155229441208
1104	18-19	0.08014052137946948
1104	20-21	0.07096741721802857
1104	22-23	0.047124466448870805
1104	24-25	0.04286044110887133
1104	26-27	0.07395964212144435
1104	28-29	0.04684481495767301
1104	30-31	0.07672666384563342
1104	32-33	0.053746523647355104
1104	34-35	0.06422546538681928
1104	36-37	0.03450394567651216
1104	38-39	0.024588933892516707
1104	40-41	0.02754576716098711
1104	42-43	0.004607775904155176
1104	44-45	0.019220365828701347
1104	46-47	-0.04976337362580807
1104	48-49	-0.07768323160041746
1104	50-51	-0.005050088797609931
1104	52-53	-0.07469176055541737
1104	54-55	-0.1342484626131082
1104	56-57	-0.12703819786162995
1104	58-59	-0.11023059106910438
1104	60-61	-0.031023689462635673
1104	62-63	-0.051380923411734614
1104	64-65	-0.05630047274139116
1104	66-67	-0.044285145860214925
1104	68-69	-0.09249849244336161
1104	70-71	-0.025582129459138514
1104	72-73	-0.08108859900683285
1104	74-75	-0.029292634058101896
1104	76-77	-0.06633277691637574
1104	78-79	-0.06559732436836896
1104	80-81	-0.023760869742794455
1104	82-83	-0.042047408289636934
1104	84-85	-0.03561284306454482
1104	86-87	-0.08227969020600057
1104	88-89	-0.06483477331045862
1104	90-91	-0.09049295848130257
1104	92-93	-0.10689765453036415
1104	94-95	-0.10598876271270896
1104	96-97	-0.08463348200406884
1104	98-99	-0.06044540501651241
1104	100	-0.015867916477247945
1105	1	0.011524665970586767
1105	2	0.1335844626888374
1105	3	0.09821757814454202
1105	4	0.16202697111074116
1105	5	0.15878400092057632
1105	6	0.0687920387694021
1105	7	0.1539454151412798
1105	8	0.13792114818394197
1105	9	0.08885189494942125
1105	10-11	0.07659311080711007
1105	12-13	0.15929296121640135
1105	14-15	0.1352546585398997
1105	16-17	0.17942653936634656
1105	18-19	0.09135641236230896
1105	20-21	0.08826830528163043
1105	22-23	0.1010808874603697
1105	24-25	0.17230514894340843
1105	26-27	0.13057259915812836
1105	28-29	0.221679180999665
1105	30-31	0.2234501733869365
1105	32-33	0.26302655231297933
1105	34-35	0.2820150518204727
1105	36-37	0.22070139935277666
1105	38-39	0.21958169615223255
1105	40-41	0.2097546442851197
1105	42-43	0.20452030844381142
1105	44-45	0.18546382497470404
1105	46-47	0.0933315258250218
1105	48-49	0.105515553293273
1105	50-51	0.09678214597953883
1105	52-53	0.11481837228099323
1105	54-55	0.12948746796603672
1105	56-57	0.12113741419110369
1105	58-59	0.14726422892571378
1105	60-61	0.16549344296551283
1105	62-63	0.17781208226431744
1105	64-65	0.20189946448396512
1105	66-67	0.1525897581121427
1105	68-69	0.12730163837372288
1105	70-71	0.14101814095469933
1105	72-73	0.15985681778604288
1105	74-75	0.2193352681714984
1105	76-77	0.1621810389915055
1105	78-79	0.07849667406885885
1105	80-81	0.09108448186103857
1105	82-83	0.13704029622179448
1105	84-85	0.22922973745599862
1105	86-87	0.14921427486846994
1105	88-89	0.21979251873208483
1105	90-91	0.2021076455599804
1105	92-93	0.16725494271545216
1105	94-95	0.19652161924682687
1105	96-97	0.20253223206842463
1105	98-99	0.18206375327654456
1105	100	0.14381918706578745
1106	1	0.0368694948298014
1106	2	0.046787129455783116
1106	3	0.07305862349767978
1106	4	0.034465233828186115
1106	5	0.0553525998376827
1106	6	0.047715190900710525
1106	7	0.042588646718570544
1106	8	0.020673841385374203
1106	9	-0.00805390468659084
1106	10-11	0.011544820429179481
1106	12-13	-0.008805237374872377
1106	14-15	-0.043823984023731555
1106	16-17	0.0022171131312802572
1106	18-19	0.018055950861963765
1106	20-21	0.014939769477621212
1106	22-23	-0.023026079242470132
1106	24-25	-0.004523213692799288
1106	26-27	-0.0771732128340048
1106	28-29	-0.07203731764186472
1106	30-31	-0.07819061941766847
1106	32-33	-0.0760415370598082
1106	34-35	-0.06750504929510015
1106	36-37	-0.05564225656718236
1106	38-39	-0.05671697219854366
1106	40-41	-0.059189157483857
1106	42-43	-0.08311672978755524
1106	44-45	-0.11412119045586167
1106	46-47	-0.07582954701517508
1106	48-49	-0.17257991341926981
1106	50-51	-0.1441004019515404
1106	52-53	-0.14611141774897618
1106	54-55	-0.08418386796386557
1106	56-57	-0.10558066021730639
1106	58-59	-0.052654218227026206
1106	60-61	-0.07758667045123957
1106	62-63	0.001923006843007613
1106	64-65	0.00650316950219576
1106	66-67	-0.04046014323286329
1106	68-69	0.035018611960353496
1106	70-71	0.03860162165666736
1106	72-73	-0.04209551393511646
1106	74-75	-0.06198397855801829
1106	76-77	-0.03126413481246715
1106	78-79	-0.04063667646964575
1106	80-81	4.4474660772664265E-4
1106	82-83	0.0363338626044758
1106	84-85	-0.0024701092600203367
1106	86-87	-0.01664544873958107
1106	88-89	-0.013083143353576077
1106	90-91	-0.05081809181117691
1106	92-93	-0.04345313758426528
1106	94-95	-0.057644710442342983
1106	96-97	-0.05607606202527293
1106	98-99	-0.09075413069021465
1106	100	-0.08779313135485367
1107	1	0.04398375857946135
1107	2	0.09017386264638816
1107	3	0.09093333368056022
1107	4	0.05289216622308146
1107	5	0.008320602802463384
1107	6	0.14609841718930738
1107	7	0.11119605439822777
1107	8	0.06318645245003296
1107	9	0.03956316305328045
1107	10-11	0.11132257625691722
1107	12-13	0.11074581791167049
1107	14-15	0.16434135492064428
1107	16-17	0.1047526109429171
1107	18-19	0.15355636587089094
1107	20-21	0.1167459388686467
1107	22-23	0.17272984190889673
1107	24-25	0.16764021559794884
1107	26-27	0.2053686938793362
1107	28-29	0.15903111595211072
1107	30-31	0.15511373220363112
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2216	30-31	-0.16885459954033877
2216	32-33	-0.1802252819489567
2216	34-35	-0.19798833412350092
2216	36-37	-0.1396446206585864
2216	38-39	-0.09750190460440677
2216	40-41	-0.14417626114460091
2216	42-43	-0.12569752658844635
2216	44-45	-0.08747417678820568
2216	46-47	-0.07642838558342646
2216	48-49	-0.09000023361451781
2216	50-51	-0.04971294529722314
2216	52-53	-0.18074532022658474
2216	54-55	-0.33055794541419914
2216	56-57	-0.20601068338032746
2216	58-59	-0.13732051115047028
2216	60-61	-0.1732608708444161
2216	62-63	-0.19589959560658343
2216	64-65	-0.23225740278085283
2216	66-67	-0.2521920750671143
2216	68-69	-0.25008698121873607
2216	70-71	-0.3637339790214469
2216	72-73	-0.3148923102729384
2216	74-75	-0.2236950603402903
2216	76-77	-0.2347518697958506
2216	78-79	-0.2555151465561778
2216	80-81	-0.16751504120534122
2216	82-83	-0.16682565299369756
2216	84-85	-0.1464444401675209
2216	86-87	-0.17505715837626923
2216	88-89	-0.1420795491823732
2216	90-91	-0.12750215685975874
2216	92-93	-0.23313664828687308
2216	94-95	-0.21350291966793122
2216	96-97	-0.3317029211582998
2216	98-99	-0.3736375772713778
2216	100	-0.375745497493817
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	60945.0
3	15155.0
4	11517.0
5	10642.0
6	6934.0
7	6171.0
8	6795.0
9	6987.0
10	6618.0
11	7124.0
12	8081.0
13	9189.0
14	9058.0
15	9021.0
16	8943.0
17	8898.0
18	8649.0
19	8476.0
20	7791.0
21	8107.0
22	10258.0
23	19207.0
24	35287.0
25	42520.0
26	42424.0
27	27016.0
28	25161.0
29	28191.0
30	33772.0
31	39843.0
32	52905.0
33	71188.0
34	103469.0
35	177462.0
36	312584.0
37	616186.0
38	927616.0
39	412807.0
40	1566.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.30163429816798	23.12407709523566	13.871718890230033	21.702569716366323
2	14.710813743286522	23.604013155000015	39.851979725148	21.83319337656546
3	16.483745209565235	31.060210081686645	30.511773368162835	21.944271340585285
4	11.244317084305775	20.820293325669255	40.05225676777568	27.88313282224929
5	11.202588309232803	42.47246816492608	34.62715509103737	11.697788434803746
6	25.69901908591564	42.64557839729735	19.122301269253022	12.533101247533985
7	23.766454271612073	37.171870236284946	23.48154803010582	15.58012746199716
8	23.151518631401537	38.71838375466483	20.92750243949019	17.20259517444344
9	24.844541985911963	20.04348174774532	21.36737520841375	33.74460105792896
10-11	21.83464640888633	30.842426876925185	28.763188615733252	18.559738098455234
12-13	22.629107307298998	29.69506294618018	27.96946104663881	19.706368699882013
14-15	20.760615409963854	30.22527564600852	26.4198269779686	22.594281966059025
16-17	20.050206533620965	33.06975085214427	26.507155577843218	20.372887036391546
18-19	20.13577202258155	32.0795066285169	28.536621061904537	19.248100286997012
20-21	22.10998816375275	30.522632883173152	27.9717858566051	19.395593096469003
22-23	21.46105412812917	30.66901789729208	27.976123421508643	19.893804553070108
24-25	20.75705798263289	31.198684500819613	27.726978686329463	20.317278830218036
26-27	20.46993615042051	31.29465702499842	27.81967463270343	20.415732191877645
28-29	20.829283119102353	30.78198181780168	28.223505045773795	20.165230017322173
30-31	20.963326356823067	30.974223318436866	27.953359007140754	20.10909131759931
32-33	20.159020122244435	30.855782832167755	28.410909693458986	20.574287352128824
34-35	20.861705636618783	30.714094246235756	28.18228257643866	20.2419175407068
36-37	21.308755671210083	30.628045071980175	27.608631816569062	20.45456744024068
38-39	20.81247895578898	30.67954975452191	27.996843875436372	20.51112741425274
40-41	20.446251173716977	30.71070844917076	28.324189600890982	20.51885077622128
42-43	20.479126171025502	30.709085763019793	28.34573700064564	20.46605106530906
44-45	20.45573764659955	30.64430313899274	28.099900048774202	20.800059165633506
46-47	20.88701329947328	30.675820696924976	27.984642523801217	20.452523479800522
48-49	20.70102850217019	30.336788510633117	28.31794850031034	20.644234486886358
50-51	20.700357583857766	30.61348770487583	27.780948603600553	20.90520610766585
52-53	20.554081788999	30.667925704690468	27.92251236752094	20.855480138789595
54-55	20.667529394803598	30.943017815533665	27.91251100384046	20.476941785822277
56-57	20.766669277527075	30.739807580627996	27.72307799846656	20.770445143378364
58-59	21.006452361835297	30.142440638551964	27.839677360064385	21.011429639548357
60-61	21.009853761651744	30.474638819708023	27.871741014297424	20.64376640434281
62-63	20.49738778653682	30.46742425329889	28.077238012558347	20.957949947605943
64-65	20.631265873859164	30.560254587133777	28.01452367422558	20.79395586478148
66-67	20.64359477407684	30.27824698718671	27.83019088718181	21.247967351554642
68-69	20.982580152114345	30.65641087411918	27.65021314918758	20.71079582457889
70-71	21.04428903410543	30.405144164742588	27.815243451291177	20.735323349860806
72-73	20.78851312955932	30.303024156491855	27.904647217108852	21.003815496839977
74-75	20.638399057843458	30.392412319558083	28.097934102091298	20.871254520507165
76-77	20.735276541606453	30.694481587661095	27.645672748515164	20.92456912221729
78-79	20.82368173133123	30.666521457059826	27.852549630011953	20.65724718159699
80-81	20.736243912196453	30.824015630212294	27.809392419496824	20.63034803809443
82-83	20.584435564049002	30.792258037135806	27.89055103318332	20.73275536563187
84-85	20.685451016945606	30.399944204282225	27.783677380921546	21.130927397850616
86-87	20.90175751750652	30.628159599565613	27.579356658719554	20.890726224208304
88-89	20.64876291478024	30.55541512662944	27.84502763017137	20.950794328418944
90-91	20.518775704192276	30.600810675780554	27.69995634246605	21.18045727756112
92-93	20.564486558766486	30.60776937467721	28.078767235501275	20.748976831055028
94-95	20.842177661302756	30.194036390053743	27.948242815888396	21.015543132755106
96-97	20.609668777805535	30.814097816187523	27.536639371286192	21.03959403472075
98-99	20.14047791387677	30.63923286376816	27.89300760698389	21.327281615371184
100	20.34991110857299	30.698889145821575	27.69062350696886	21.26057623863658
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	34728.0
1	23275.5
2	9125.5
3	4995.0
4	2823.0
5	1917.0
6	1738.5
7	2043.5
8	2626.0
9	2810.5
10	2068.5
11	1301.5
12	1180.5
13	1187.0
14	1255.0
15	1381.5
16	1631.0
17	1946.0
18	2429.0
19	3167.0
20	4112.5
21	5510.5
22	7849.5
23	11306.0
24	15693.5
25	21164.0
26	28165.5
27	36291.5
28	46159.5
29	56123.5
30	63001.0
31	70078.5
32	78006.0
33	83991.0
34	90645.0
35	99771.5
36	107211.5
37	112179.0
38	120042.5
39	125068.0
40	126954.0
41	127932.5
42	125115.5
43	124630.5
44	122603.5
45	115821.5
46	111375.5
47	107506.5
48	98526.5
49	95112.0
50	95006.0
51	87476.0
52	77290.5
53	68505.5
54	62235.5
55	58340.5
56	52009.0
57	44464.5
58	41084.0
59	37901.5
60	33396.5
61	28073.5
62	22667.0
63	17136.0
64	12579.5
65	9853.5
66	7665.0
67	5958.0
68	4434.5
69	3234.0
70	2543.5
71	2089.5
72	1617.0
73	1117.0
74	752.5
75	567.5
76	433.0
77	346.0
78	275.5
79	194.5
80	141.5
81	114.5
82	103.0
83	96.0
84	78.0
85	67.0
86	64.5
87	54.0
88	46.5
89	46.0
90	37.0
91	25.0
92	22.0
93	25.5
94	19.5
95	17.0
96	17.5
97	16.0
98	15.5
99	13.0
100	45.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.1843852032242775E-4
2	6.241100580640792E-5
3	0.0
4	6.241100580640792E-5
5	0.0
6	3.120550290320396E-5
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	3.120550290320396E-5
64-65	6.241100580640792E-5
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	3.120550290320396E-5
84-85	4.992880464512634E-4
86-87	0.0013418366248377704
88-89	0.0029645227758043763
90-91	0.002371618220643501
92-93	0.0018099191683858297
94-95	0.0019035356770954416
96-97	0.0016382889024182082
98-99	0.0022936044633854913
100	0.0028709062670947646
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3204563.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.81513613493559
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.89054687567027	42.14765794479513
2	13.683863877730277	15.001657226194254
3	4.470807253346966	7.352037246758143
4	1.902645891921093	4.171751743289227
5	0.9251190440967734	2.535526317159306
6	0.5384503250239094	1.7709136720851546
7	0.33025476897647094	1.2672072084459873
8	0.21510003762641364	0.9432590276097306
9	0.14779731543627941	0.7291376969415907
>10	0.7482444891136342	7.574667459222077
>50	0.0687453824264362	2.616557482645431
>100	0.06823196082155963	7.526416276748753
>500	0.007117861599529831	2.6977988242413167
>1k	0.003017973317456509	2.4549279481516537
>5k	0.0	0.0
>10k+	5.694289278219828E-5	1.2104839257122604
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	38781	1.2101806080891528	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.120550290320396E-5	0.0
5	0.0	0.0	0.0	3.120550290320396E-5	0.0
6	0.0	0.0	0.0	3.120550290320396E-5	0.0
7	0.0	0.0	0.0	3.120550290320396E-5	0.0
8	0.0	0.0	0.0	3.120550290320396E-5	0.0
9	0.0	0.0	0.0	3.120550290320396E-5	0.0
10-11	0.0	0.0	0.0	1.4042476306441783E-4	0.0
12-13	0.0	0.0	0.0	1.8723301741922378E-4	0.0
14-15	0.0	0.0	0.0	2.0283576887082576E-4	0.0
16-17	0.0	0.0	0.0	2.9645227758043764E-4	0.0
18-19	0.0	0.0	0.0	3.120550290320396E-4	0.0
20-21	0.0	0.0	0.0	3.9006878629004954E-4	0.0
22-23	0.0	0.0	0.0	7.021238153220892E-4	0.0
24-25	0.0	0.0	0.0	0.0012170146132249545	0.0
26-27	0.0	1.560275145160198E-5	0.0	0.0021687824517726754	0.0
28-29	0.0	3.120550290320396E-5	0.0	0.004384373157900157	0.0
30-31	0.0	3.120550290320396E-5	0.0	0.010032569183380073	0.0
32-33	0.0	3.120550290320396E-5	0.0	0.01923819253982524	0.0
34-35	0.0	3.120550290320396E-5	0.0	0.03332747710062183	0.0
36-37	0.0	3.120550290320396E-5	0.0	0.05464083558351014	0.0
38-39	0.0	3.120550290320396E-5	0.0	0.08244493867026487	0.0
40-41	0.0	3.120550290320396E-5	0.0	0.11391568834814607	0.0
42-43	0.0	3.120550290320396E-5	0.0	0.14357651885764144	0.0
44-45	0.0	3.120550290320396E-5	0.0	0.1803366012776157	0.0
46-47	0.0	3.120550290320396E-5	0.0	0.2250384841864554	0.0
48-49	0.0	3.120550290320396E-5	0.0	0.2732977944262603	0.0
50-51	0.0	3.120550290320396E-5	0.0	0.3260506970841266	0.0
52-53	0.0	3.120550290320396E-5	0.0	0.37682205030763943	0.0
54-55	0.0	3.120550290320396E-5	0.0	0.4321182014521169	0.0
56-57	0.0	3.120550290320396E-5	0.0	0.4983050731098124	0.0
58-59	0.0	3.120550290320396E-5	0.0	0.5735883488637921	0.0
60-61	0.0	3.120550290320396E-5	0.0	0.6575467544248623	0.0
62-63	0.0	3.120550290320396E-5	0.0	0.7427221745991575	0.0
64-65	0.0	3.120550290320396E-5	0.0	0.8306748845318379	0.0
66-67	0.0	3.120550290320396E-5	0.0	0.9302048360416069	0.0
68-69	0.0	3.120550290320396E-5	0.0	1.0466793756278157	0.0
70-71	0.0	3.120550290320396E-5	0.0	1.1811750931406249	0.0
72-73	0.0	3.120550290320396E-5	0.0	1.3277317375255222	0.0
74-75	0.0	3.120550290320396E-5	0.0	1.4757862460497733	0.0
76-77	0.0	3.120550290320396E-5	0.0	1.6305031294438586	0.0
78-79	0.0	3.120550290320396E-5	0.0	1.8093418665821206	0.0
80-81	0.0	3.120550290320396E-5	0.0	2.012848553765365	0.0
82-83	0.0	3.120550290320396E-5	0.0	2.233237418019243	0.0
84-85	0.0	3.120550290320396E-5	0.0	2.462036165305535	0.0
86-87	0.0	3.120550290320396E-5	0.0	2.702724209197947	0.0
88	0.0	3.120550290320396E-5	0.0	2.895777052908618	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4185	0.0	51.773144	1
GTATCAA	8355	0.0	43.99055	1
TCAACGC	9945	0.0	35.586273	4
ATCAACG	9945	0.0	35.539013	3
CAACGCA	10845	0.0	32.719727	5
AACGCAG	11325	0.0	31.291433	6
ACGCAGA	12700	0.0	27.64453	7
TATCAAC	13250	0.0	27.6321	2
CGCAGAG	12670	0.0	27.413223	8
GTACATG	19885	0.0	24.912315	1
TACATGG	18675	0.0	24.36167	2
GCAGAGT	13935	0.0	24.115229	9
CGCAGAA	415	0.0	22.650312	2
ACATGGG	19325	0.0	22.472158	3
CATGGGT	2985	0.0	20.46875	4
AATCCGC	725	0.0	20.096296	6
GTGGTAT	2165	0.0	19.321072	1
CAGAGTA	13565	0.0	19.31601	10-11
TGGTATC	2050	0.0	19.029026	2
AGAGTAC	13090	0.0	18.939787	10-11
>>END_MODULE