FastQCFastQC Report
Wed 25 May 2016
SRR2431368_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431368_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3204563
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT327801.0229163851670258No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT221270.6904841627391941No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193330.6032959876276422No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA62220.19416063906373507No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53950.1683536881627854No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53080.16563880941020664No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36160.11283909849798553No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33520.10460084573153969No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79700.064.403371
GTATCAA186650.053.0112951
ATCAACG260700.037.751353
TCAACGC263800.037.3433574
CAACGCA274450.035.8760035
TATCAAC281550.035.1560172
AACGCAG283450.034.736886
ACGCAGA314600.031.1330997
CGCAGAG314400.031.1080558
GCAGAGT331650.029.2349599
GTGGTAT25300.026.5680581
GCGTATC1258.023104E-626.3228151
TGGTATC25650.025.6529622
TACATGG179150.024.975662
GTACATG189100.024.8323751
AGAGTAC322500.024.373610-11
AGTACTT235550.023.97308712-13
ACATGGG181800.023.9135863
GAGTACT230050.022.81992712-13
CAGAGTA328400.022.16110410-11
Produced by FastQC (version 0.11.5)