##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431368_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3204563
Sequences flagged as poor quality	0
Sequence length	100
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.744093032341695	34.0	31.0	34.0	31.0	34.0
2	32.872318628156165	34.0	31.0	34.0	31.0	34.0
3	32.94367531547983	34.0	31.0	34.0	31.0	34.0
4	36.33813846068871	37.0	37.0	37.0	35.0	37.0
5	36.30947246161177	37.0	37.0	37.0	35.0	37.0
6	36.349595561079624	37.0	37.0	37.0	35.0	37.0
7	36.21709387520233	37.0	37.0	37.0	35.0	37.0
8	36.269695431171115	37.0	37.0	37.0	35.0	37.0
9	38.0763820215112	39.0	39.0	39.0	35.0	39.0
10-11	38.06456824222211	39.0	39.0	39.0	35.0	39.0
12-13	38.05129997444269	39.0	39.0	39.0	35.0	39.0
14-15	39.48942289479096	41.0	39.0	41.0	36.0	41.0
16-17	39.51601669244761	41.0	39.0	41.0	36.5	41.0
18-19	39.61408778669666	41.0	40.0	41.0	37.0	41.0
20-21	39.61259195091499	41.0	40.0	41.0	37.0	41.0
22-23	39.53279807574387	41.0	40.0	41.0	36.5	41.0
24-25	39.49611163831075	41.0	40.0	41.0	36.0	41.0
26-27	39.393507788737494	41.0	39.0	41.0	36.0	41.0
28-29	39.29659129809587	41.0	39.0	41.0	35.5	41.0
30-31	39.0672520714993	41.0	39.0	41.0	35.0	41.0
32-33	38.81085174484009	41.0	38.0	41.0	35.0	41.0
34-35	38.68732398146018	41.0	38.0	41.0	35.0	41.0
36-37	38.56011256449007	41.0	38.0	41.0	35.0	41.0
38-39	38.42097253198018	41.0	38.0	41.0	35.0	41.0
40-41	38.299678614525604	41.0	38.0	41.0	35.0	41.0
42-43	38.12957055298959	41.0	38.0	41.0	34.5	41.0
44-45	37.85101978023212	40.0	38.0	41.0	33.5	41.0
46-47	37.43910979437758	40.0	38.0	41.0	33.0	41.0
48-49	37.06822661935496	40.0	37.5	41.0	32.0	41.0
50-51	36.88404986889008	40.0	37.0	41.0	31.0	41.0
52-53	36.774254555145276	40.0	37.0	41.0	31.0	41.0
54-55	36.62457892074519	40.0	36.5	41.0	31.0	41.0
56-57	36.44937546866765	40.0	36.0	41.0	31.0	41.0
58-59	36.25836424498442	40.0	35.5	41.0	30.5	41.0
60-61	36.05870706863931	39.5	35.0	41.0	30.0	41.0
62-63	35.829065304692094	39.0	35.0	41.0	30.0	41.0
64-65	35.564242456771794	39.0	35.0	41.0	29.5	41.0
66-67	35.26938602860983	38.0	35.0	41.0	29.0	41.0
68-69	34.99442934964924	37.0	35.0	40.0	29.0	41.0
70-71	34.72822815466571	37.0	35.0	40.0	29.0	41.0
72-73	34.453879983011724	36.5	35.0	39.0	29.0	41.0
74-75	34.15530167451849	36.0	35.0	39.0	29.0	41.0
76-77	33.83521247670899	35.5	35.0	39.0	29.0	40.5
78-79	33.511255512842155	35.0	35.0	37.0	28.0	39.5
80-81	33.202088397076295	35.0	34.5	37.0	28.0	39.0
82-83	32.8817337964646	35.0	34.0	36.5	27.0	39.0
84-85	32.62546921374303	35.0	34.0	36.0	27.0	37.5
86-87	32.40368203090406	35.0	34.0	36.0	27.0	37.0
88-89	32.17959141386829	35.0	34.0	35.5	26.0	37.0
90-91	31.985342151176305	35.0	34.0	35.0	26.0	36.0
92-93	31.84642305362697	35.0	34.0	35.0	26.0	36.0
94-95	31.709090287817716	35.0	34.0	35.0	25.0	36.0
96-97	31.569006756927543	35.0	34.0	35.0	25.0	36.0
98-99	31.450870368284225	35.0	34.0	35.0	24.5	36.0
100	31.34538032174746	35.0	34.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2554858173149199
1101	2	0.2552110719710896
1101	3	0.2536522238678174
1101	4	0.17644845910101026
1101	5	0.19746230766808992
1101	6	0.22390477623974903
1101	7	0.22924062745960327
1101	8	0.2372505540065859
1101	9	0.3157019291200882
1101	10-11	0.332821525315552
1101	12-13	0.28468611572517233
1101	14-15	0.39787570022125607
1101	16-17	0.40250260730758214
1101	18-19	0.3937298334294468
1101	20-21	0.3793831181659044
1101	22-23	0.4037709079139873
1101	24-25	0.3758000526678984
1101	26-27	0.3830910701380361
1101	28-29	0.3837515542546157
1101	30-31	0.4059191111501619
1101	32-33	0.40201486631571015
1101	34-35	0.4308810493673434
1101	36-37	0.4123990732974576
1101	38-39	0.38452944633045405
1101	40-41	0.47174768343508333
1101	42-43	0.50899658743743
1101	44-45	0.565102988790656
1101	46-47	0.6903681007676568
1101	48-49	0.5966201889262734
1101	50-51	0.5972608134214141
1101	52-53	0.5665522139003727
1101	54-55	0.6077162017490565
1101	56-57	0.60109922142518
1101	58-59	0.602558873424492
1101	60-61	0.6043613699588022
1101	62-63	0.5561267258255924
1101	64-65	0.5881303355510283
1101	66-67	0.598234596331018
1101	68-69	0.5186660068951738
1101	70-71	0.4928992458627661
1101	72-73	0.42358076642671705
1101	74-75	0.47036849244057777
1101	76-77	0.46612178504132373
1101	78-79	0.4026541153738492
1101	80-81	0.44186012500603766
1101	82-83	0.5187808937348919
1101	84-85	0.45889006675385957
1101	86-87	0.46575578619962954
1101	88-89	0.441765349687131
1101	90-91	0.46390524641042674
1101	92-93	0.49815829456685634
1101	94-95	0.5182653279227125
1101	96-97	0.4998899357987696
1101	98-99	0.5352041285852103
1101	100	0.5434037857380503
1102	1	0.12632277435550776
1102	2	0.19278754820247457
1102	3	0.19907846498027482
1102	4	0.13265821266563194
1102	5	0.14350763515226816
1102	6	0.12331721175522148
1102	7	0.16167565977317366
1102	8	0.18880027825556311
1102	9	0.22065528953371683
1102	10-11	0.22418416439896305
1102	12-13	0.24378879558039301
1102	14-15	0.3099879198860265
1102	16-17	0.3041129158669875
1102	18-19	0.30880985478166423
1102	20-21	0.26359410175356146
1102	22-23	0.29388205822128555
1102	24-25	0.29741155166606603
1102	26-27	0.2958948503195842
1102	28-29	0.27793573696165197
1102	30-31	0.2723873907594907
1102	32-33	0.27995072407777855
1102	34-35	0.33136437041790856
1102	36-37	0.2774458118445011
1102	38-39	0.2880684694371851
1102	40-41	0.32714488249856544
1102	42-43	0.32986225762826393
1102	44-45	0.3496413575926596
1102	46-47	0.3706980356748204
1102	48-49	0.28748215973404
1102	50-51	0.26563629818321743
1102	52-53	0.21488772436865844
1102	54-55	0.21914258024222022
1102	56-57	0.19161404398867887
1102	58-59	0.2724125105787607
1102	60-61	0.20873381662773483
1102	62-63	0.2567390159270815
1102	64-65	0.33787856793864535
1102	66-67	0.3395070647712899
1102	68-69	0.3302417432499496
1102	70-71	0.29887380772891703
1102	72-73	0.29487083240437784
1102	74-75	0.2727702826975005
1102	76-77	0.2702780171774677
1102	78-79	0.22110361690309333
1102	80-81	0.21536350480432276
1102	82-83	0.26026692805444895
1102	84-85	0.23290171151528227
1102	86-87	0.2126193147649218
1102	88-89	0.2077529568993839
1102	90-91	0.2598286048564873
1102	92-93	0.2671377887497144
1102	94-95	0.29699303120833065
1102	96-97	0.2821482796394008
1102	98-99	0.3111016291877071
1102	100	0.34738203418058333
1103	1	0.1485740832560296
1103	2	0.21787480823738292
1103	3	0.1953699134968545
1103	4	0.1282952109204274
1103	5	0.17404864736867154
1103	6	0.17480063234859244
1103	7	0.14182400183251076
1103	8	0.18247392572502008
1103	9	0.21192928604331485
1103	10-11	0.2570157525316006
1103	12-13	0.27923818476014617
1103	14-15	0.36419821657014495
1103	16-17	0.4151513102823401
1103	18-19	0.33138838881308175
1103	20-21	0.30831486964187604
1103	22-23	0.3151516917291417
1103	24-25	0.3272981136206923
1103	26-27	0.2949131749269185
1103	28-29	0.3210203791955024
1103	30-31	0.3801535338659505
1103	32-33	0.4063686996449647
1103	34-35	0.3623416828088324
1103	36-37	0.3402730369753897
1103	38-39	0.36998382720332756
1103	40-41	0.3843002053607023
1103	42-43	0.3679294478551398
1103	44-45	0.4716963314146483
1103	46-47	0.41563695365038456
1103	48-49	0.3859769241319455
1103	50-51	0.38114676938740644
1103	52-53	0.36181180813829883
1103	54-55	0.35352303399440643
1103	56-57	0.35086360768850255
1103	58-59	0.3843235053431542
1103	60-61	0.2782146194200479
1103	62-63	0.3785758396618064
1103	64-65	0.35096757317424476
1103	66-67	0.3500873440034056
1103	68-69	0.32958729298817246
1103	70-71	0.3343231969086631
1103	72-73	0.37133243798901816
1103	74-75	0.36864724604828325
1103	76-77	0.28674834876559885
1103	78-79	0.28611234290657706
1103	80-81	0.2368512883994356
1103	82-83	0.32767530501780584
1103	84-85	0.2779497045344783
1103	86-87	0.28897184530593734
1103	88-89	0.2889047893601173
1103	90-91	0.35199701672385686
1103	92-93	0.40380881841812055
1103	94-95	0.35578447972490324
1103	96-97	0.3736622412449826
1103	98-99	0.37251087875140954
1103	100	0.3687607427320678
1104	1	0.07910209460865048
1104	2	0.10335703010704123
1104	3	0.12881099975207633
1104	4	0.03228823893178401
1104	5	0.060917995298225946
1104	6	0.0686529866067076
1104	7	0.03786556934004892
1104	8	0.07896391285636639
1104	9	0.13291157842613188
1104	10-11	0.14258611585065495
1104	12-13	0.14226527062754712
1104	14-15	0.16194168528874542
1104	16-17	0.2618463364603585
1104	18-19	0.23386813831508135
1104	20-21	0.22034639783832688
1104	22-23	0.2107117865693411
1104	24-25	0.2564615851598191
1104	26-27	0.19447984952631714
1104	28-29	0.18174532058144166
1104	30-31	0.1735356534915269
1104	32-33	0.14609305929734262
1104	34-35	0.25639786416867594
1104	36-37	0.2835336315776118
1104	38-39	0.24638031829223195
1104	40-41	0.3188100066898727
1104	42-43	0.36516676430057515
1104	44-45	0.3600679401723852
1104	46-47	0.4282516477114129
1104	48-49	0.41332501234391117
1104	50-51	0.3311937047092073
1104	52-53	0.31997326564530226
1104	54-55	0.3612949062950719
1104	56-57	0.3854745513308657
1104	58-59	0.39311726757278365
1104	60-61	0.3611039402020424
1104	62-63	0.34650226799399775
1104	64-65	0.3235368523534703
1104	66-67	0.37220203365735216
1104	68-69	0.3260274802006151
1104	70-71	0.26895309096264697
1104	72-73	0.34203587286130244
1104	74-75	0.33782418119380253
1104	76-77	0.34792787967680283
1104	78-79	0.3381670302688633
1104	80-81	0.34702424395851494
1104	82-83	0.3867466026362223
1104	84-85	0.36997213212627855
1104	86-87	0.40090762985297346
1104	88-89	0.4117519344585645
1104	90-91	0.3966709594671656
1104	92-93	0.39005985408015675
1104	94-95	0.3400605960363592
1104	96-97	0.35748993793262684
1104	98-99	0.34440752849491574
1104	100	0.3037013353951714
1105	1	-0.019678793367155833
1105	2	0.04581013569573855
1105	3	0.06483766141433733
1105	4	-0.09651911974845717
1105	5	-0.07366035008520555
1105	6	-0.07132869680718557
1105	7	-0.45327464637643544
1105	8	-0.36301591365735675
1105	9	-0.31817002201987066
1105	10-11	-0.26407995818830443
1105	12-13	-0.17465364692696284
1105	14-15	-0.17205053482635435
1105	16-17	-0.07565637974998651
1105	18-19	-0.10686631947446301
1105	20-21	-0.055871342362472376
1105	22-23	-0.06820954319944406
1105	24-25	-0.07705516096286402
1105	26-27	-0.12174012400298295
1105	28-29	-0.15944622876688896
1105	30-31	-0.12661793585698433
1105	32-33	-0.14394319525163013
1105	34-35	-0.1293997578577546
1105	36-37	-0.11349222784231472
1105	38-39	-0.1824669055761845
1105	40-41	-0.177603223833529
1105	42-43	-0.08174216089225439
1105	44-45	-0.11868265245533394
1105	46-47	-0.12980652528320036
1105	48-49	-0.14454261437965243
1105	50-51	-0.11310752137167412
1105	52-53	-0.1758577630247089
1105	54-55	-0.13679332060671356
1105	56-57	-0.10997318987958948
1105	58-59	-0.12497475104890299
1105	60-61	-0.1350321819934024
1105	62-63	-0.14933315051091256
1105	64-65	-0.07614526872695393
1105	66-67	-0.13845593674637513
1105	68-69	-0.08837732403470255
1105	70-71	-0.08924975452833195
1105	72-73	-0.0900131868360603
1105	74-75	-0.13292360523194446
1105	76-77	-0.12166743790579915
1105	78-79	-0.1264521350918315
1105	80-81	-0.10988362767393767
1105	82-83	-0.13528241197754198
1105	84-85	-0.11753151692397523
1105	86-87	-0.141328206344717
1105	88-89	-0.09686064724723309
1105	90-91	-0.1298325315490345
1105	92-93	-0.09211302639781138
1105	94-95	-0.1008427606508917
1105	96-97	-0.14379175667533772
1105	98-99	-0.11785155187856944
1105	100	-0.15834305461531883
1106	1	0.08459767001236429
1106	2	0.05293894832783508
1106	3	0.086924583236069
1106	4	0.08102938741117782
1106	5	0.078068211406368
1106	6	0.09336083487737312
1106	7	0.05303803680410368
1106	8	0.06422304640175014
1106	9	0.06011035733866521
1106	10-11	0.07170893232036235
1106	12-13	0.10883693684855444
1106	14-15	0.10698314592433888
1106	16-17	0.12748327179553343
1106	18-19	0.12757759725320028
1106	20-21	0.0863581883364759
1106	22-23	0.10067589492538787
1106	24-25	0.07380210979241753
1106	26-27	0.07808414115855555
1106	28-29	0.09240736210826128
1106	30-31	0.10367540541614062
1106	32-33	0.06293952507456169
1106	34-35	0.1041083916747425
1106	36-37	0.11838155936064254
1106	38-39	0.07749058632219175
1106	40-41	0.1352831305266875
1106	42-43	0.1539185236296703
1106	44-45	0.15253491034826538
1106	46-47	0.12733890012491855
1106	48-49	0.1497666212420583
1106	50-51	0.18179822881235452
1106	52-53	0.19002128635018067
1106	54-55	0.21838383855192234
1106	56-57	0.1867924185317804
1106	58-59	0.1785978886791426
1106	60-61	0.21251670807568956
1106	62-63	0.2177043287703384
1106	64-65	0.2562637160117873
1106	66-67	0.25314484116081104
1106	68-69	0.27399690179705516
1106	70-71	0.22422023627324705
1106	72-73	0.20408622126833365
1106	74-75	0.20998697201522987
1106	76-77	0.2035897682184924
1106	78-79	0.21666037109180536
1106	80-81	0.1925318270327665
1106	82-83	0.24513200080332354
1106	84-85	0.2534404000152364
1106	86-87	0.22217654679801058
1106	88-89	0.22524051777627108
1106	90-91	0.21861276159219756
1106	92-93	0.25131031469845055
1106	94-95	0.31382135820832247
1106	96-97	0.28411115637279494
1106	98-99	0.23382284423628974
1106	100	0.2704493502467038
1107	1	-0.02867235531696366
1107	2	0.030484897932581134
1107	3	0.015582787395956643
1107	4	-0.06997176420031082
1107	5	-0.07122612027612263
1107	6	-0.06744540036377344
1107	7	-0.016045635733945574
1107	8	0.0016569282819389741
1107	9	0.012211359179403303
1107	10-11	0.024624523179753055
1107	12-13	-4.956721333968517E-4
1107	14-15	0.03112919997044372
1107	16-17	0.03505370080144132
1107	18-19	0.009074171515301543
1107	20-21	0.050365581927273695
1107	22-23	0.010731362170311343
1107	24-25	0.018057681784988233
1107	26-27	-0.03319368817629709
1107	28-29	-0.017435929907946957
1107	30-31	-0.0016669539785283405
1107	32-33	-0.002958992225643442
1107	34-35	0.00675805522654116
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2216	42-43	-0.9339064381893323
2216	44-45	-0.9026091215014347
2216	46-47	-0.7878592734723995
2216	48-49	-0.856402319703129
2216	50-51	-0.7549679843012314
2216	52-53	-0.717962370335357
2216	54-55	-0.8135929238575415
2216	56-57	-0.8285544327887848
2216	58-59	-0.9484965706861672
2216	60-61	-0.8907635481300034
2216	62-63	-0.8734973278684635
2216	64-65	-0.7768195605286934
2216	66-67	-0.752527211538613
2216	68-69	-0.8178963032760223
2216	70-71	-0.7898560319305474
2216	72-73	-0.7898711715161184
2216	74-75	-0.6821187920512486
2216	76-77	-0.6543753790593314
2216	78-79	-0.6604126561134791
2216	80-81	-0.721036484730945
2216	82-83	-0.7714838664200272
2216	84-85	-0.7593723680648594
2216	86-87	-0.7614870385495749
2216	88-89	-0.6771722427486289
2216	90-91	-0.689896970155317
2216	92-93	-0.8965609039500393
2216	94-95	-0.8469505146237815
2216	96-97	-0.9390019444532882
2216	98-99	-0.9848658486934134
2216	100	-0.9708667577902688
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	49.0
3	560.0
4	364.0
5	436.0
6	613.0
7	935.0
8	1540.0
9	2188.0
10	2798.0
11	4200.0
12	6722.0
13	10450.0
14	10839.0
15	14150.0
16	19337.0
17	31546.0
18	38869.0
19	19199.0
20	10540.0
21	8767.0
22	8366.0
23	8500.0
24	9401.0
25	11175.0
26	13775.0
27	17073.0
28	20713.0
29	25542.0
30	32187.0
31	40013.0
32	50644.0
33	64874.0
34	90649.0
35	158632.0
36	258578.0
37	579125.0
38	1056371.0
39	569588.0
40	5255.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.627546527036145	22.23058260456529	15.461419245468386	22.680451622930182
2	13.269056198337307	22.790048309269196	41.05411006374976	22.886785428643737
3	14.757253195687511	30.295145688025755	32.81468585478224	22.13291526150449
4	9.883810375587103	19.446135641002677	41.29105199776689	29.379001985643328
5	10.605689449700318	39.88060150479176	36.14006028279051	13.373648762717414
6	25.6347901414327	41.792718695185584	19.14651077229563	13.425980391086087
7	23.140253444853478	35.74303266935304	24.617834007320187	16.49887987847329
8	24.86741561954001	34.50130329782875	21.658678578015163	18.972602504616074
9	26.12281300133591	15.590612510972635	22.823860850917892	35.462713636773564
10-11	22.54819143827099	27.707225602991738	30.000127942561903	19.744455016175372
12-13	24.355380125152788	26.73743658651741	29.767085246880775	19.140098041449022
14-15	21.58526763243537	26.825420501952994	29.7906766070756	21.79863525853603
16-17	19.145184538422242	30.14024065059729	29.53894181515545	21.175632995825016
18-19	19.12644563392887	27.7195517766385	34.311573840177275	18.842428749255358
20-21	20.717661035217596	26.542667440147067	34.26985208279569	18.469819441839654
22-23	20.016470274711576	26.781952348543214	34.25798416694206	18.943593209803154
24-25	19.487914177310742	27.25897981954156	33.96398663716912	19.28911936597858
26-27	19.237500042907726	27.37942070511594	34.065536794390226	19.3175424575861
28-29	19.579705995473613	26.941755336370164	34.38183453644319	19.09670413171303
30-31	19.747528687971453	27.06020943837967	34.16499650103877	19.027265372610117
32-33	19.08659010240122	26.980352971208198	34.4317939967308	19.50126292965978
34-35	19.708564726214586	27.04035196423436	33.98531370687924	19.26576960267181
36-37	20.16497987253045	26.903184523154742	33.332428342260755	19.599407262054054
38-39	19.824939600448623	27.04572079086433	33.52972419358563	19.599615415101418
40-41	19.43294487571728	27.18164138321868	33.72260231651556	19.662811424548472
42-43	19.640870293122052	27.165477863133773	33.50399158696885	19.68966025677532
44-45	19.758393496496154	27.20298126148465	32.89454570759478	20.144079534424417
46-47	20.324931146820678	27.404384540501088	32.23040299881712	20.04028131386112
48-49	20.43605297057634	27.336794928125986	31.823959212306708	20.40319288899097
50-51	20.569636201678463	27.707860403524815	30.956707779736632	20.765795615060085
52-53	20.448154709394075	27.901355036552566	30.9433922815685	20.70709797248486
54-55	20.54109001263828	28.252890421432024	30.89956468146854	20.306454884461157
56-57	20.6355638195242	27.88811687666297	30.85617111095959	20.620148192853236
58-59	20.771526548403312	27.269801029351143	31.069531599357536	20.889140822888006
60-61	20.894552917796116	27.627291253484692	31.000057574377436	20.478098254341756
62-63	20.372340940090737	27.483263708655436	31.373435317077554	20.770960034176266
64-65	20.466425531343898	27.691061152487872	31.312771819433728	20.5297414967345
66-67	20.612362365212736	27.4370693707881	31.029659322452	20.920908941547157
68-69	20.915468641487113	27.725032371995457	30.75794391371379	20.60155507280364
70-71	20.954609958956187	27.49994733971966	30.900350366398527	20.645092334925625
72-73	20.71651078190983	27.374824761296995	30.935815631331316	20.972848825461863
74-75	20.556214611294337	27.48139686668796	31.09571991017009	20.86666861184762
76-77	20.66580279149741	27.811808357017085	30.673377952147764	20.84901089933774
78-79	20.659504404315456	27.85159434581229	30.79386898692553	20.69503226294672
80-81	20.49143379793958	27.935650076617826	30.855521764970124	20.717394360472476
82-83	20.390535136865793	27.831963291918992	30.893242869616287	20.884258701598927
84-85	20.454983754258034	27.522006333530136	30.71478144078449	21.30822847142734
86-87	20.522521440167637	27.756063044585723	30.52092215539899	21.200493359847655
88-89	20.236999317215286	27.622292733647807	30.87419535631501	21.266512592821897
90-91	20.125060871060388	27.67823694004671	30.763067598746087	21.433634590146823
92-93	20.11060144254339	27.684578366717332	31.08990572258392	21.114914468155355
94-95	20.35429297901747	27.265898856085602	30.925741522217194	21.454066642679738
96-97	20.13617113699904	27.811431872147175	30.50885989283185	21.543537098021936
98-99	19.669925738596238	27.483350709987953	30.953652233693273	21.893071317722534
100	19.957465598781702	27.62164667112919	30.676821247852605	21.744066482236505
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5379.0
1	3290.5
2	2886.5
3	4020.5
4	2219.0
5	1501.0
6	3772.0
7	10282.0
8	17379.0
9	17136.5
10	9832.0
11	4311.0
12	3511.0
13	3582.0
14	3777.0
15	4113.0
16	4547.5
17	5081.5
18	5774.5
19	6526.0
20	7466.0
21	8858.0
22	11078.5
23	14553.0
24	18820.0
25	23820.0
26	30148.5
27	37889.5
28	47806.0
29	57487.0
30	63818.0
31	70469.0
32	78882.0
33	84820.0
34	90628.5
35	99358.0
36	106823.0
37	111313.5
38	118096.0
39	123072.0
40	124864.5
41	126485.5
42	124124.5
43	123033.5
44	121890.5
45	116309.0
46	111906.5
47	107813.5
48	97432.5
49	93519.0
50	94305.5
51	86140.0
52	75192.5
53	66046.5
54	59344.0
55	54346.5
56	47477.0
57	40521.5
58	38140.5
59	35990.0
60	31703.0
61	26095.0
62	20663.0
63	15727.0
64	11426.5
65	8653.0
66	6560.0
67	4907.0
68	3538.0
69	2451.0
70	1852.5
71	1573.5
72	1122.0
73	606.0
74	400.5
75	332.0
76	222.0
77	157.0
78	101.5
79	47.5
80	24.0
81	10.0
82	4.5
83	5.0
84	4.5
85	3.5
86	3.0
87	2.5
88	4.5
89	3.5
90	3.5
91	4.5
92	2.5
93	1.5
94	4.0
95	6.5
96	8.0
97	6.5
98	1.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009268034362251576
2	6.241100580640792E-5
3	3.120550290320396E-4
4	0.0018723301741922376
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	6.241100580640792E-5
24-25	7.80137572580099E-5
26-27	3.7446603483844756E-4
28-29	6.397128095156812E-4
30-31	0.0012638228675797606
32-33	0.003401399816449232
34-35	0.00457160617531938
36-37	0.003479413573707242
38-39	0.0021531797003210736
40-41	0.0029645227758043763
42-43	3.276577804836416E-4
44-45	4.212742891932535E-4
46-47	0.003104947538868794
48-49	0.0020907686945146656
50-51	8.26945826934905E-4
52-53	0.0
54-55	4.0567153774165147E-4
56-57	7.021238153220892E-4
58-59	6.241100580640792E-4
60-61	3.900687862900495E-4
62-63	0.0
64-65	0.0
66-67	7.021238153220892E-4
68-69	5.773018037092733E-4
70-71	0.0018879329256438397
72-73	1.716302659676218E-4
74-75	0.0019503439314502477
76-77	3.276577804836416E-4
78-79	0.0011077953530637405
80-81	6.709183124188852E-4
82-83	0.0011546036074185466
84-85	9.673705899993229E-4
86-87	1.716302659676218E-4
88-89	0.0012170146132249545
90-91	0.0013574393762893723
92-93	0.002621262243869133
94-95	0.0012482201161281585
96-97	0.0020595631916114614
98-99	0.0022467962090306852
100	0.0030581392845139884
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3204563.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.61429999525494
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.57676056652207	37.746505319882466
2	15.034868382569872	15.219586774091297
3	4.877477066252737	7.40610262353876
4	2.067085969025779	4.1849643740901215
5	1.0469724708524974	2.6495889363250806
6	0.5757791151415306	1.7485594118865515
7	0.364035793639414	1.2897791807793215
8	0.23908841128061814	0.9681034059156881
9	0.17873627759089827	0.8141950416618798
>10	0.8618477992005352	8.067354326961262
>50	0.08679909388467992	3.080038093730995
>100	0.07691810123949733	7.817974313560147
>500	0.009621849035646673	3.3493954069251957
>1k	0.0036390326476205543	2.8118500668009974
>5k	1.850355583535875E-4	0.5283672034351443
>10k+	1.850355583535875E-4	2.3176355204150734
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32780	1.0229163851670258	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22127	0.6904841627391941	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19333	0.6032959876276422	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6222	0.19416063906373507	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5395	0.1683536881627854	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5308	0.16563880941020664	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3616	0.11283909849798553	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3352	0.10460084573153969	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	6.241100580640792E-5	0.0
5	0.0	0.0	0.0	6.241100580640792E-5	0.0
6	0.0	0.0	0.0	6.241100580640792E-5	0.0
7	0.0	0.0	0.0	6.241100580640792E-5	0.0
8	0.0	0.0	0.0	6.241100580640792E-5	0.0
9	0.0	0.0	0.0	9.361650870961189E-5	0.0
10-11	0.0	0.0	0.0	1.560275145160198E-4	0.0
12-13	0.0	0.0	0.0	2.1843852032242775E-4	0.0
14-15	0.0	0.0	0.0	2.340412717740297E-4	0.0
16-17	0.0	0.0	0.0	2.9645227758043764E-4	0.0
18-19	0.0	0.0	0.0	3.120550290320396E-4	0.0
20-21	0.0	0.0	0.0	3.9006878629004954E-4	0.0
22-23	0.0	0.0	0.0	7.333293182252931E-4	0.0
24-25	0.0	0.0	0.0	0.0013106311219345664	0.0
26-27	0.0	0.0	0.0	0.0022936044633854913	0.0
28-29	0.0	0.0	0.0	0.004509195169512973	0.0
30-31	0.0	0.0	0.0	0.01032902146096051	0.0
32-33	0.0	0.0	0.0	0.019753083337728107	0.0
34-35	0.0	0.0	0.0	0.03399839541304071	0.0
36-37	0.0	0.0	0.0	0.055561397919154654	0.0
38-39	0.0	0.0	0.0	0.08347472026607061	0.0
40-41	0.0	0.0	0.0	0.11517951121572582	0.0
42-43	0.0	0.0	0.0	0.144965163736834	0.0
44-45	0.0	0.0	0.0	0.18242736997213035	0.0
46-47	0.0	0.0	0.0	0.22747251341290528	0.0
48-49	0.0	0.0	0.0	0.27615309794190346	0.0
50-51	0.0	0.0	0.0	0.329093233617189	0.0
52-53	0.0	0.0	0.0	0.38034827213570155	0.0
54-55	0.0	0.0	0.0	0.4361281085751786	0.0
56-57	0.0	0.0	0.0	0.5030795150540026	0.0
58-59	0.0	0.0	0.0	0.5788464761029819	0.0
60-61	0.0	0.0	0.0	0.6629609091785682	0.0
62-63	0.0	0.0	0.0	0.7487292339080243	0.0
64-65	0.0	0.0	0.0	0.8370252043726398	0.0
66-67	0.0	0.0	0.0	0.9378501842528919	0.0
68-69	0.0	0.0	0.0	1.0560098209958735	0.0
70-71	0.0	0.0	0.0	1.1914729090986822	0.0
72-73	0.0	0.0	0.0	1.340557199218739	0.0
74-75	0.0	0.0	0.0	1.4907648874433113	0.0
76-77	0.0	0.0	0.0	1.6474945257746532	0.0
78-79	0.0	0.0	0.0	1.8287828948908167	0.0
80-81	0.0	0.0	0.0	2.034536378283092	0.0
82-83	0.0	0.0	0.0	2.258170614838903	0.0
84-85	0.0	0.0	0.0	2.49130692702874	0.0
86-87	0.0	0.0	0.0	2.7356460147608272	0.0
88	0.0	0.0	0.0	2.931881819767625	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7970	0.0	64.40337	1
GTATCAA	18665	0.0	53.011295	1
ATCAACG	26070	0.0	37.75135	3
TCAACGC	26380	0.0	37.343357	4
CAACGCA	27445	0.0	35.876003	5
TATCAAC	28155	0.0	35.156017	2
AACGCAG	28345	0.0	34.73688	6
ACGCAGA	31460	0.0	31.133099	7
CGCAGAG	31440	0.0	31.108055	8
GCAGAGT	33165	0.0	29.234959	9
GTGGTAT	2530	0.0	26.568058	1
GCGTATC	125	8.023104E-6	26.322815	1
TGGTATC	2565	0.0	25.652962	2
TACATGG	17915	0.0	24.97566	2
GTACATG	18910	0.0	24.832375	1
AGAGTAC	32250	0.0	24.3736	10-11
AGTACTT	23555	0.0	23.973087	12-13
ACATGGG	18180	0.0	23.913586	3
GAGTACT	23005	0.0	22.819927	12-13
CAGAGTA	32840	0.0	22.161104	10-11
>>END_MODULE