FastQCFastQC Report
Wed 25 May 2016
SRR2431367_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431367_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4619755
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA302920.6557057679465685No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60100.046.609291
GTATCAA107500.040.6170121
TCAACGC129150.032.898044
ATCAACG130700.032.5438543
TATCAAC136950.031.4704762
CAACGCA143550.029.5979235
AACGCAG146850.028.9648086
GTACATG259900.026.6377281
ACGCAGA160500.026.2378947
TACATGG261950.025.7292272
CGCAGAG162800.025.5785168
ACATGGG273750.023.9505833
GCAGAGT186350.021.6650549
CATGGGG172100.021.0834
GTGGTAT30450.019.911461
CATGGGA73350.018.7102154
AGAGTAC165050.018.65184410-11
TATACCG3559.094947E-1118.5351335
CATGGGT39600.017.921644
CAGAGTA178250.017.91661510-11
Produced by FastQC (version 0.11.5)