##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431367_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4619755
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.00175940066086	33.0	31.0	34.0	27.0	34.0
2	31.096714869078554	34.0	31.0	34.0	27.0	34.0
3	31.201674764137923	34.0	31.0	34.0	27.0	34.0
4	34.37163031372876	37.0	35.0	37.0	31.0	37.0
5	34.52539669311468	37.0	35.0	37.0	31.0	37.0
6	34.55726569915504	37.0	35.0	37.0	31.0	37.0
7	34.48225284674187	37.0	35.0	37.0	31.0	37.0
8	34.4563172722363	37.0	35.0	37.0	31.0	37.0
9	35.92297318797209	39.0	37.0	39.0	31.0	39.0
10-11	35.93997993400083	39.0	37.0	39.0	31.0	39.0
12-13	35.87532942764281	39.0	37.0	39.0	30.0	39.0
14-15	37.243088323947916	41.0	38.0	41.0	31.0	41.0
16-17	37.25965662681246	41.0	38.0	41.0	31.0	41.0
18-19	37.286931341597125	41.0	38.0	41.0	31.0	41.0
20-21	37.341029232069666	41.0	38.0	41.0	31.0	41.0
22-23	37.3570930709529	41.0	38.0	41.0	30.0	41.0
24-25	37.380935352632335	41.0	38.0	41.0	30.0	41.0
26-27	37.38271769823291	41.0	38.0	41.0	30.0	41.0
28-29	37.35619215737631	40.0	38.0	41.0	30.0	41.0
30-31	37.28969479983246	40.0	38.0	41.0	30.0	41.0
32-33	37.21110426851641	40.0	38.0	41.0	30.0	41.0
34-35	37.137729489983776	40.0	38.0	41.0	30.0	41.0
36-37	37.03784605460679	40.0	38.0	41.0	30.0	41.0
38-39	36.93739646366528	40.0	38.0	41.0	30.0	41.0
40-41	36.83777429755474	40.0	37.0	41.0	30.0	41.0
42-43	36.71307060655814	40.0	37.0	41.0	29.0	41.0
44-45	36.576895095086215	40.0	37.0	41.0	29.0	41.0
46-47	36.44749061367973	40.0	36.5	41.0	29.0	41.0
48-49	36.317659161578916	40.0	36.0	41.0	29.0	41.0
50-51	36.1675532576944	40.0	36.0	41.0	28.5	41.0
52-53	35.98528374340198	40.0	35.0	41.0	28.0	41.0
54-55	35.81115697693926	39.5	35.0	41.0	28.0	41.0
56-57	35.60384371032663	39.0	35.0	41.0	28.0	41.0
58-59	35.37151082687285	39.0	35.0	41.0	28.0	41.0
60-61	35.18496099901402	39.0	35.0	41.0	27.5	41.0
62-63	35.013543142439374	38.0	34.5	41.0	27.0	41.0
64-65	34.75290637706978	38.0	34.0	40.0	27.0	41.0
66-67	34.45498440934638	37.0	34.0	40.0	26.0	41.0
68-69	34.14640191958232	37.0	34.0	40.0	26.0	41.0
70-71	33.904220894831	36.0	34.0	39.5	26.0	41.0
72-73	33.663933693453444	36.0	34.0	39.0	26.0	41.0
74-75	33.32472566185869	35.5	33.5	39.0	26.0	41.0
76-77	32.98477657364947	35.0	33.0	38.5	25.0	40.0
78-79	32.65539633595288	35.0	33.0	37.0	25.0	39.0
80-81	32.32744409606137	35.0	33.0	37.0	24.0	39.0
82-83	32.00746846964829	35.0	33.0	36.0	24.0	39.0
84-85	31.72318348050925	35.0	33.0	36.0	23.0	37.0
86-87	31.463985536029508	35.0	32.5	36.0	23.0	37.0
88-89	31.22056548453327	35.0	32.0	35.0	21.0	37.0
90-91	31.017899866984287	35.0	32.0	35.0	20.0	36.0
92-93	30.815958963191772	35.0	32.0	35.0	19.5	36.0
94-95	30.607577999266194	35.0	32.0	35.0	18.5	36.0
96-97	30.42911831904506	35.0	32.0	35.0	13.5	36.0
98-99	30.313757439517897	35.0	32.0	35.0	2.0	35.0
100	30.272111399846963	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5039971528517349
1101	2	0.5062986048756279
1101	3	0.5144964912993437
1101	4	0.5418049428323073
1101	5	0.46076395439908424
1101	6	0.508589512933554
1101	7	0.4609185788207242
1101	8	0.4943493037986144
1101	9	0.6268499477456544
1101	10-11	0.5520193397423157
1101	12-13	0.6282386657056591
1101	14-15	0.7074766621807882
1101	16-17	0.666668235179877
1101	18-19	0.6484553716372119
1101	20-21	0.5792618578798212
1101	22-23	0.5822143459015123
1101	24-25	0.6100215666516462
1101	26-27	0.606133151055829
1101	28-29	0.6623731978646106
1101	30-31	0.6805615569696144
1101	32-33	0.6590740211105768
1101	34-35	0.6401864592971123
1101	36-37	0.6442836804738192
1101	38-39	0.5943576250905167
1101	40-41	0.6638227247442714
1101	42-43	0.6913363516400537
1101	44-45	0.651294853121307
1101	46-47	0.6306009701418986
1101	48-49	0.589483122095821
1101	50-51	0.6290804885671051
1101	52-53	0.64901279058423
1101	54-55	0.6777777371360258
1101	56-57	0.6527387572876862
1101	58-59	0.6261728688927377
1101	60-61	0.6932515891229372
1101	62-63	0.6962869999870591
1101	64-65	0.7107062939568252
1101	66-67	0.6345157242201509
1101	68-69	0.6407141891872357
1101	70-71	0.6358316462238136
1101	72-73	0.695713298740074
1101	74-75	0.6936069879139595
1101	76-77	0.7071214011600588
1101	78-79	0.6639383426596481
1101	80-81	0.6705702710910586
1101	82-83	0.6797066481766905
1101	84-85	0.6816712936459552
1101	86-87	0.664566277513245
1101	88-89	0.6657096871350845
1101	90-91	0.7016013769041471
1101	92-93	0.6982242650518948
1101	94-95	0.7318037489118012
1101	96-97	0.6455353196135789
1101	98-99	0.6948751186724387
1101	100	0.7311853926963678
1102	1	0.15752977278154745
1102	2	0.24189063742389294
1102	3	0.26117954657917153
1102	4	0.23690140152577044
1102	5	0.2516102356687284
1102	6	0.2068978950705116
1102	7	0.19229625453553467
1102	8	0.19616836081338818
1102	9	0.27691282230183845
1102	10-11	0.29916787114296994
1102	12-13	0.319390701040426
1102	14-15	0.3694667409950583
1102	16-17	0.44420406225641074
1102	18-19	0.3218176331746534
1102	20-21	0.34329699828390403
1102	22-23	0.320957234976035
1102	24-25	0.3464010193325109
1102	26-27	0.3663414919310952
1102	28-29	0.42168401145173817
1102	30-31	0.38132431513616183
1102	32-33	0.39395504900755185
1102	34-35	0.47438622675061026
1102	36-37	0.513168571892912
1102	38-39	0.4808517810095765
1102	40-41	0.47236711275519383
1102	42-43	0.45988573993673043
1102	44-45	0.39215876584176357
1102	46-47	0.39353777893974495
1102	48-49	0.31955334504984023
1102	50-51	0.38306553227645423
1102	52-53	0.36371276022413923
1102	54-55	0.3159512575265637
1102	56-57	0.35191854020759195
1102	58-59	0.3412716223541281
1102	60-61	0.3263642539117768
1102	62-63	0.4150711546338499
1102	64-65	0.40313776425494297
1102	66-67	0.4076407061710441
1102	68-69	0.4343775866420856
1102	70-71	0.3963381929683294
1102	72-73	0.39283497985254456
1102	74-75	0.47387505337391644
1102	76-77	0.46499465077815927
1102	78-79	0.404965015627063
1102	80-81	0.43922997264562724
1102	82-83	0.4121399680616449
1102	84-85	0.3622981420962006
1102	86-87	0.37876986780182875
1102	88-89	0.34172971136156605
1102	90-91	0.2586267359568879
1102	92-93	0.3609748130841339
1102	94-95	0.36699174324073525
1102	96-97	0.3178362146094429
1102	98-99	0.3171362395497006
1102	100	0.3303318428026323
1103	1	0.09014999297723136
1103	2	0.12250623156679907
1103	3	0.13744840840639228
1103	4	0.1422272755589944
1103	5	0.1299804321442437
1103	6	0.0912591277096908
1103	7	0.15696802662187537
1103	8	0.14841009784351655
1103	9	0.12401497212203338
1103	10-11	0.20757721161926668
1103	12-13	0.19020798150098273
1103	14-15	0.22803658872209098
1103	16-17	0.1822093065607504
1103	18-19	0.18960120226829957
1103	20-21	0.254053107920889
1103	22-23	0.2562600054723134
1103	24-25	0.2784609878252624
1103	26-27	0.3133290929183872
1103	28-29	0.28598275648792537
1103	30-31	0.2700924992443632
1103	32-33	0.24708345728992498
1103	34-35	0.2834834687396395
1103	36-37	0.2855436802086331
1103	38-39	0.20307361744103503
1103	40-41	0.16723638839192034
1103	42-43	0.13007427850534015
1103	44-45	0.1141667031724154
1103	46-47	0.0713278908855628
1103	48-49	0.12472982737884308
1103	50-51	0.02421952597919841
1103	52-53	0.07257860412082806
1103	54-55	0.04488499407408142
1103	56-57	-0.012449038649791078
1103	58-59	0.020431239788422317
1103	60-61	0.05217512563216076
1103	62-63	0.008565560301953212
1103	64-65	0.022683188038378432
1103	66-67	0.043434992388782234
1103	68-69	-0.006603407348357848
1103	70-71	-0.008344468440000696
1103	72-73	-0.02515933599392639
1103	74-75	-0.009683602443907091
1103	76-77	-0.02513249837863185
1103	78-79	-0.032855242406341745
1103	80-81	0.00763343032990349
1103	82-83	0.03654380202315366
1103	84-85	-0.014621043632988773
1103	86-87	0.03627787777251257
1103	88-89	0.030653577551959188
1103	90-91	0.06542577069409106
1103	92-93	0.05671421790174236
1103	94-95	0.04996207451552337
1103	96-97	0.0495279931522461
1103	98-99	0.01623971989461026
1103	100	-0.008077773056680115
1104	1	0.19483682386476175
1104	2	0.2556756617891871
1104	3	0.2547441123787486
1104	4	0.18889675584647136
1104	5	0.22833181292449467
1104	6	0.22197989496542903
1104	7	0.2732869995893168
1104	8	0.2570436184683018
1104	9	0.2746716191210652
1104	10-11	0.2821386479440591
1104	12-13	0.31529000695326204
1104	14-15	0.24612130734338677
1104	16-17	0.28114375621615295
1104	18-19	0.20125105648838115
1104	20-21	0.21185870261096085
1104	22-23	0.20807314668874
1104	24-25	0.27355971303149573
1104	26-27	0.3086741023162176
1104	28-29	0.33587399886685887
1104	30-31	0.37972480445146317
1104	32-33	0.35740501184221074
1104	34-35	0.3623127918781677
1104	36-37	0.35369311395589875
1104	38-39	0.34785050359229785
1104	40-41	0.3547245879960599
1104	42-43	0.37530326992111185
1104	44-45	0.3363255742805009
1104	46-47	0.39539941449355354
1104	48-49	0.3405094876161172
1104	50-51	0.31207512663643655
1104	52-53	0.30319057680708994
1104	54-55	0.25586768947478333
1104	56-57	0.291435286803555
1104	58-59	0.23418645089866175
1104	60-61	0.2888213265789972
1104	62-63	0.3141186141427923
1104	64-65	0.35737139617759084
1104	66-67	0.37668152007033484
1104	68-69	0.34922750771206523
1104	70-71	0.3349513086901936
1104	72-73	0.3198966399112848
1104	74-75	0.331077329572139
1104	76-77	0.3100308200238544
1104	78-79	0.3134300299237083
1104	80-81	0.34219315040142106
1104	82-83	0.3201819273425244
1104	84-85	0.29469375010071275
1104	86-87	0.32547985510318966
1104	88-89	0.2656572372911725
1104	90-91	0.28310732682776774
1104	92-93	0.2892914299009597
1104	94-95	0.34125022789720916
1104	96-97	0.37056217337990205
1104	98-99	0.33548183335058823
1104	100	0.2943576900933884
1105	1	0.1902564304505816
1105	2	0.23689507495647888
1105	3	0.21242126224964153
1105	4	0.23349683382814135
1105	5	0.17207844664059735
1105	6	0.19665734035765325
1105	7	0.24559006776747339
1105	8	0.2688650336896359
1105	9	0.225072867052603
1105	10-11	0.2532505244932963
1105	12-13	0.2862806457812468
1105	14-15	0.26619716437398466
1105	16-17	0.2403269084661943
1105	18-19	0.24524871479080446
1105	20-21	0.26688311776712936
1105	22-23	0.26915883926114503
1105	24-25	0.29317934261479195
1105	26-27	0.3116585307015498
1105	28-29	0.27376545226999127
1105	30-31	0.3152758009838621
1105	32-33	0.34865801106538896
1105	34-35	0.363809828424678
1105	36-37	0.3632967145968351
1105	38-39	0.320042400127619
1105	40-41	0.27985248805666174
1105	42-43	0.26744427985497765
1105	44-45	0.259049250034046
1105	46-47	0.26662819332749166
1105	48-49	0.2614495479114609
1105	50-51	0.2520501646347171
1105	52-53	0.2581824949165892
1105	54-55	0.1940235061886284
1105	56-57	0.23645888450050734
1105	58-59	0.270125557959787
1105	60-61	0.3112069740553878
1105	62-63	0.3101110508192022
1105	64-65	0.3297744696743834
1105	66-67	0.34120571012089584
1105	68-69	0.3127880931439506
1105	70-71	0.32126336161584845
1105	72-73	0.3256148279197859
1105	74-75	0.3205589799071049
1105	76-77	0.3282904287745865
1105	78-79	0.2446926429540639
1105	80-81	0.17664183643640996
1105	82-83	0.24456503123793993
1105	84-85	0.31469692068132105
1105	86-87	0.3024310871537317
1105	88-89	0.27623120789185407
1105	90-91	0.28514889139786703
1105	92-93	0.2559928893044159
1105	94-95	0.26691776339909623
1105	96-97	0.2615363141394482
1105	98-99	0.2735639101876366
1105	100	0.27738131906541597
1106	1	0.13589136532320722
1106	2	0.15084178730676712
1106	3	0.16184091050294214
1106	4	0.14066456804059868
1106	5	0.11428806785923484
1106	6	0.08093696379521731
1106	7	0.14018312225236684
1106	8	0.11424068173253943
1106	9	0.12862648736695803
1106	10-11	0.1045057696218521
1106	12-13	0.1469003677781089
1106	14-15	0.1915066709365263
1106	16-17	0.15249296150303593
1106	18-19	0.15901341952568515
1106	20-21	0.15417666539686792
1106	22-23	0.18097477800765915
1106	24-25	0.2183219834634329
1106	26-27	0.2700325182079837
1106	28-29	0.25284737799068324
1106	30-31	0.24128749553920414
1106	32-33	0.2756790096932775
1106	34-35	0.2646246260095708
1106	36-37	0.2523455253817204
1106	38-39	0.17672094968541785
1106	40-41	0.2074116501110126
1106	42-43	0.1932017733436382
1106	44-45	0.2163037056501338
1106	46-47	0.22050182483052083
1106	48-49	0.2167041839676358
1106	50-51	0.21330890753162635
1106	52-53	0.16832721840260234
1106	54-55	0.2736710510539382
1106	56-57	0.27350924513685015
1106	58-59	0.26436389571297525
1106	60-61	0.24092329646350663
1106	62-63	0.3036734458164787
1106	64-65	0.30890988734615377
1106	66-67	0.3298148418870923
1106	68-69	0.3552211983784943
1106	70-71	0.3432372760057376
1106	72-73	0.2722160142262595
1106	74-75	0.294972889259995
1106	76-77	0.2693668239892233
1106	78-79	0.25181513869473093
1106	80-81	0.2531890143778526
1106	82-83	0.28569529069196875
1106	84-85	0.2785633798651119
1106	86-87	0.32383702239594214
1106	88-89	0.34281455745202294
1106	90-91	0.29538976976836295
1106	92-93	0.3054624067734899
1106	94-95	0.2927803270974074
1106	96-97	0.283360758040871
1106	98-99	0.2801561454640904
1106	100	0.3479376597404773
1107	1	-0.03615481101339313
1107	2	0.10545988217934976
1107	3	0.0318252061643598
1107	4	0.0793087819572662
1107	5	0.06887068334343383
1107	6	0.10783321427636139
1107	7	0.07928087810434192
1107	8	0.121690654192804
1107	9	0.040622728843281664
1107	10-11	0.09328752491245496
1107	12-13	0.10063679887827703
1107	14-15	0.14355885061090845
1107	16-17	0.1225789433830684
1107	18-19	0.14036104865100185
1107	20-21	0.07968985474783352
1107	22-23	0.09653575617226551
1107	24-25	0.08339948165271238
1107	26-27	0.08431529072002775
1107	28-29	0.0641352978492975
1107	30-31	0.10810832891954902
1107	32-33	0.1001114797848004
1107	34-35	0.10739428882934732
1107	36-37	0.1240705151850392
1107	38-39	0.1505035556462957
1107	40-41	0.13015103873959788
1107	42-43	0.13116494612688
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2216	80-81	-0.8357164216727888
2216	82-83	-0.8173944547312857
2216	84-85	-0.767483021299249
2216	86-87	-0.8377119068214043
2216	88-89	-0.8645446831314771
2216	90-91	-0.8075741765282629
2216	92-93	-0.909114567024961
2216	94-95	-0.8773672704406188
2216	96-97	-1.0197100076306214
2216	98-99	-1.1030268871039972
2216	100	-1.0856775118073436
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	91181.0
3	19321.0
4	14082.0
5	12837.0
6	8520.0
7	7700.0
8	8781.0
9	8790.0
10	8882.0
11	9929.0
12	11062.0
13	12291.0
14	12855.0
15	12731.0
16	12879.0
17	12911.0
18	12660.0
19	12206.0
20	11736.0
21	12443.0
22	15433.0
23	25345.0
24	42381.0
25	52158.0
26	50663.0
27	38473.0
28	38827.0
29	46080.0
30	54770.0
31	65588.0
32	84099.0
33	113417.0
34	163643.0
35	263793.0
36	470484.0
37	883347.0
38	1269020.0
39	624079.0
40	4358.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.54371780934952	21.41119569014893	13.873956597576228	22.171129902925315
2	14.932003969907495	22.506020340905526	39.62673345231511	22.93524223687187
3	17.353972234458322	29.386731547452193	29.875891686896814	23.38340453119267
4	12.085987243912285	19.430792325567047	38.762640010130404	29.72058042039026
5	12.496030633659144	40.72739788148939	33.86391702590289	12.91265445894858
6	27.742748262624318	40.33376228825988	18.737075883894274	13.186413565221534
7	24.799258835154678	35.73873506279013	22.957667668523545	16.504338433531647
8	23.94345587590684	37.56467604883809	20.90567573388632	17.586192341368754
9	25.154991985505724	18.93797398346882	21.089018789957475	34.81801524106798
10-11	22.810603592614758	29.39826895582125	28.371125741516597	19.420001710047394
12-13	23.52042478443121	27.56261966273103	28.125430894062564	20.791524658775195
14-15	21.572799856269434	28.598464637193967	25.70733729386082	24.12139821267578
16-17	20.925644325294307	31.58283285585491	26.24082878853965	21.25069403031113
18-19	20.99346177448804	30.556544665247397	27.895710919734924	20.55428264052964
20-21	22.920263087544683	29.294334872736762	27.08626323257402	20.699138807144536
22-23	22.49242654642941	29.55754147135508	26.957111361966163	20.992920620249343
24-25	21.841060835477204	29.511532537980912	27.234929125029357	21.412477501512527
26-27	21.781036007320733	29.583830744271072	27.202048593486015	21.433084654922176
28-29	21.546315767827515	29.53355751549595	27.47640296942154	21.443723747254996
30-31	21.87411453637693	29.602673734862563	27.116232354313162	21.406979374447346
32-33	21.529496694088756	29.60928663965946	27.480364218448816	21.380852447802965
34-35	21.959530321413148	29.17571386361398	27.396377080602758	21.468378734370113
36-37	21.932223678528405	29.40861582486517	27.227158150161646	21.43200234644478
38-39	21.68398540615249	29.373689730299553	27.356872821177745	21.585452042370214
40-41	21.800203690455447	29.199189134488734	27.39267558561006	21.607931589445762
42-43	21.649286596367123	29.421051549270473	27.425642701831592	21.504019152530816
44-45	21.844426814841913	29.260804956106977	27.32193590352735	21.572832325523756
46-47	21.698347639647558	29.28448586559244	27.45974624195439	21.557420252805613
48-49	21.609132951855674	29.192143306300878	27.41938695883223	21.77933678301122
50-51	21.731953317870754	29.21184132058951	27.224712133002726	21.83149322853701
52-53	21.59220564726917	29.267266337717047	27.398108774166595	21.742419240847187
54-55	21.749356838187307	29.21975299555929	27.288059648184802	21.742830518068597
56-57	21.60423009445306	29.24646436878146	27.29433703735371	21.854968499411765
58-59	21.777334512328032	28.98982738262094	27.44621738598692	21.786620719064107
60-61	21.998287787988758	29.28234289480719	27.033359994198825	21.686009323005223
62-63	21.67160848824418	29.21325464516076	27.414955428362635	21.700181438232423
64-65	21.941952307839834	28.967760830503273	27.341407646685873	21.74887921497102
66-67	21.697005577135585	29.133493009910698	27.270645304783482	21.89885610817024
68-69	21.818234949688893	29.034028860837857	27.41348837763041	21.734247811842835
70-71	21.880197110019903	29.02663669393723	27.295689922950462	21.797476273092403
72-73	21.839296672659046	29.211321812520364	27.40127993800537	21.54810157681522
74-75	21.940037945735217	29.006538225511957	27.267052040638518	21.786371788114305
76-77	21.845335953962927	29.00009849007144	27.185402689103643	21.96916286686199
78-79	21.84822571759758	29.039137356851175	27.34608220565809	21.766554719893154
80-81	21.67734003210127	29.093631588688147	27.400911953123053	21.82811642608753
82-83	21.668228800259755	29.163039125493807	27.589263488284	21.579468585962445
84-85	21.802080707591674	28.821284117144163	27.306086193630723	22.070548981633436
86-87	21.94805570103763	29.029816556210324	27.24596637637716	21.776161366374883
88-89	21.707490696982976	29.00886407242526	27.355172364901016	21.928472865690747
90-91	21.799898974626007	28.837938697058412	27.225905356303855	22.13625697201173
92-93	21.71714640811653	29.027740903358985	27.413866198118324	21.841246490406164
94-95	21.687793589071575	28.817498089956217	27.45147720334739	22.04323111762482
96-97	21.753543304615153	29.025577295835507	27.26779775193583	21.953081647613512
98-99	21.603351515117406	28.97820870102056	27.402036781205126	22.016403002656915
100	21.63576224884298	29.03225806451613	27.289712140825433	22.042267545815456
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	25321.0
1	17313.0
2	7379.5
3	4404.5
4	2905.5
5	2217.0
6	1918.0
7	1958.5
8	2171.0
9	2268.5
10	1919.5
11	1557.0
12	1535.0
13	1612.5
14	1765.0
15	1935.5
16	2292.0
17	3074.5
18	4033.0
19	5359.5
20	8175.0
21	11290.0
22	14009.0
23	18310.5
24	25454.0
25	32593.5
26	40356.0
27	50559.5
28	62905.0
29	76709.0
30	87029.5
31	96433.5
32	106086.5
33	113621.5
34	121418.0
35	129466.5
36	134596.0
37	137543.5
38	140711.5
39	143410.0
40	146238.5
41	147440.0
42	145266.0
43	143774.5
44	142381.5
45	141975.5
46	144686.0
47	141604.0
48	137151.0
49	137148.0
50	137523.0
51	135959.5
52	131822.5
53	129159.0
54	128166.5
55	125278.5
56	114059.0
57	99137.5
58	90509.0
59	85763.0
60	76765.5
61	64390.0
62	54062.0
63	44009.0
64	34777.0
65	27282.5
66	21053.0
67	15996.0
68	11938.5
69	8875.0
70	6373.0
71	4818.0
72	3655.5
73	2510.0
74	1827.0
75	1348.0
76	946.0
77	695.0
78	481.0
79	340.5
80	244.0
81	181.0
82	156.5
83	135.5
84	102.5
85	88.5
86	83.0
87	78.0
88	60.5
89	49.0
90	44.0
91	41.5
92	41.0
93	35.5
94	35.5
95	26.0
96	21.5
97	22.0
98	21.5
99	25.5
100	66.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.8140020412337885E-4
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	2.164616954795222E-5
64-65	4.329233909590444E-5
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	1.0823084773976109E-4
84-85	6.169158321166382E-4
86-87	0.0012230085794593002
88-89	0.003279394686514761
90-91	0.002673301939172099
92-93	0.002088855361377389
94-95	0.002088855361377389
96-97	0.001980624513637628
98-99	0.0024460171589186005
100	0.0029222328889735496
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4619755.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.116431173367246
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.1143711806275	33.736533151765876
2	14.975446549243607	14.411300863542403
3	5.910993566517282	8.532477453286361
4	2.911337533388013	5.603326881908203
5	1.650834104959642	3.9716122794968967
6	1.061987946308416	3.0659441955296725
7	0.6927636167563881	2.33333190195502
8	0.48367024281714244	1.8617988759293487
9	0.3559786369940697	1.5415579427502812
>10	1.674849348822165	14.073597644212121
>50	0.10447503336780126	3.455307435140218
>100	0.059687553542467964	5.323680610928629
>500	0.0027485738607935634	0.9080911940705854
>1k	8.110542265197594E-4	0.5246915938029348
>5k	0.0	0.0
>10k+	4.505856813994545E-5	0.6567479756814636
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	30292	0.6557057679465685	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	2.164616954795222E-5	0.0
18-19	0.0	0.0	0.0	4.329233909590444E-5	0.0
20-21	0.0	0.0	0.0	8.658467819180887E-5	0.0
22-23	0.0	0.0	0.0	2.489309498014505E-4	0.0
24-25	0.0	0.0	0.0	4.5456956050699655E-4	0.0
26-27	0.0	0.0	0.0	7.035005103084471E-4	0.0
28-29	0.0	0.0	0.0	0.002251201632987031	0.0
30-31	0.0	0.0	0.0	0.00614751215161843	0.0
32-33	0.0	0.0	0.0	0.013301571187216638	0.0
34-35	0.0	0.0	0.0	0.02361597097681587	0.0
36-37	0.0	0.0	0.0	0.03896310518631399	0.0
38-39	0.0	0.0	0.0	0.06077162100587585	0.0
40-41	0.0	0.0	0.0	0.08506944632345222	0.0
42-43	0.0	0.0	0.0	0.10764640116196639	0.0
44-45	0.0	2.164616954795222E-5	0.0	0.136814614627832	0.0
46-47	0.0	2.164616954795222E-5	0.0	0.17021465424032228	0.0
48-49	0.0	2.164616954795222E-5	0.0	0.20681832694590946	0.0
50-51	0.0	2.164616954795222E-5	0.0	0.2453160394869425	0.0
52-53	0.0	2.164616954795222E-5	0.0	0.283586467247722	0.0
54-55	0.0	2.164616954795222E-5	0.0	0.329357292756867	0.0
56-57	0.0	2.164616954795222E-5	0.0	0.38075612234847955	0.0
58-59	0.0	2.164616954795222E-5	0.0	0.44003415765554665	0.0
60-61	0.0	2.164616954795222E-5	0.0	0.505145835655787	0.0
62-63	0.0	2.164616954795222E-5	0.0	0.5720000303046373	0.0
64-65	0.0	2.164616954795222E-5	0.0	0.6417981040120093	0.0
66-67	0.0	2.164616954795222E-5	0.0	0.7239128481921661	0.0
68-69	0.0	2.164616954795222E-5	0.0	0.8201084256632657	0.0
70-71	0.0	2.164616954795222E-5	0.0	0.9277331806556841	0.0
72-73	0.0	2.164616954795222E-5	0.0	1.043323726041749	0.0
74-75	0.0	2.164616954795222E-5	0.0	1.159866702887924	0.0
76-77	0.0	2.164616954795222E-5	0.0	1.2900467665493083	0.0
78-79	0.0	2.164616954795222E-5	0.0	1.4391455823956032	0.0
80-81	0.0	2.164616954795222E-5	0.0	1.6133214856632008	0.0
82-83	0.0	2.164616954795222E-5	0.0	1.8024224228341112	0.0
84-85	0.0	2.164616954795222E-5	0.0	1.9945754699112832	0.0
86-87	0.0	2.164616954795222E-5	0.0	2.197172793795342	0.0
88	0.0	2.164616954795222E-5	0.0	2.3633937297540673	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6010	0.0	46.60929	1
GTATCAA	10750	0.0	40.617012	1
TCAACGC	12915	0.0	32.89804	4
ATCAACG	13070	0.0	32.543854	3
TATCAAC	13695	0.0	31.470476	2
CAACGCA	14355	0.0	29.597923	5
AACGCAG	14685	0.0	28.964808	6
GTACATG	25990	0.0	26.637728	1
ACGCAGA	16050	0.0	26.237894	7
TACATGG	26195	0.0	25.729227	2
CGCAGAG	16280	0.0	25.578516	8
ACATGGG	27375	0.0	23.950583	3
GCAGAGT	18635	0.0	21.665054	9
CATGGGG	17210	0.0	21.083	4
GTGGTAT	3045	0.0	19.91146	1
CATGGGA	7335	0.0	18.710215	4
AGAGTAC	16505	0.0	18.651844	10-11
TATACCG	355	9.094947E-11	18.535133	5
CATGGGT	3960	0.0	17.92164	4
CAGAGTA	17825	0.0	17.916615	10-11
>>END_MODULE