FastQCFastQC Report
Wed 25 May 2016
SRR2431367_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431367_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4619755
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT343730.7440437858717617No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240770.5211748242060456No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192860.4174680259018065No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58650.12695478439873975No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50990.11037381852500837No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104400.063.8857271
GTATCAA227400.049.7516561
TATCAAC305750.036.7849272
ATCAACG308050.036.3882263
TCAACGC310150.036.1569984
CAACGCA325100.034.4646265
AACGCAG334750.033.443026
ACGCAGA373150.029.8503347
GTGGTAT33150.029.350041
CGCAGAG377950.029.322018
GCAGAGT404850.027.0138539
GTACATG240450.025.2361681
TACATGG245000.024.4014022
TGGTATC38200.024.3610252
ACATGGG249200.023.1225723
AGAGTAC382700.023.04487610-11
AGTACTT271050.022.53259312-13
GAGTACT262500.022.34444612-13
CAGAGTA397150.021.65021110-11
GTACTTT291350.020.16412214-15
Produced by FastQC (version 0.11.5)