##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431367_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4619755
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.750938956719565	34.0	31.0	34.0	31.0	34.0
2	32.882610008539416	34.0	31.0	34.0	31.0	34.0
3	32.95346181778038	34.0	31.0	34.0	31.0	34.0
4	36.336484077618834	37.0	37.0	37.0	35.0	37.0
5	36.291057426205505	37.0	37.0	37.0	35.0	37.0
6	36.32138912128457	37.0	37.0	37.0	35.0	37.0
7	36.18946589158949	37.0	37.0	37.0	35.0	37.0
8	36.244366205567175	37.0	37.0	37.0	35.0	37.0
9	38.059191883552266	39.0	39.0	39.0	35.0	39.0
10-11	38.052078627546265	39.0	39.0	39.0	35.0	39.0
12-13	38.026639940862665	39.0	39.0	39.0	35.0	39.0
14-15	39.49204232691993	41.0	39.0	41.0	36.0	41.0
16-17	39.512651320253994	41.0	39.0	41.0	36.5	41.0
18-19	39.59312809878446	41.0	40.0	41.0	37.0	41.0
20-21	39.60012587247592	41.0	40.0	41.0	37.0	41.0
22-23	39.52683183415571	41.0	40.0	41.0	36.0	41.0
24-25	39.48935138335258	41.0	40.0	41.0	36.0	41.0
26-27	39.39445944644251	41.0	39.0	41.0	36.0	41.0
28-29	39.304082900500134	41.0	39.0	41.0	36.0	41.0
30-31	39.12823602117429	41.0	39.0	41.0	35.0	41.0
32-33	38.917563701971204	41.0	38.5	41.0	35.0	41.0
34-35	38.81140439265718	41.0	38.0	41.0	35.0	41.0
36-37	38.70199123113672	41.0	38.0	41.0	35.0	41.0
38-39	38.584204248926625	41.0	38.0	41.0	35.0	41.0
40-41	38.47427352749226	41.0	38.0	41.0	35.0	41.0
42-43	38.32558122671007	41.0	38.0	41.0	35.0	41.0
44-45	38.094869424893744	40.0	38.0	41.0	34.0	41.0
46-47	37.75363466244423	40.0	38.0	41.0	33.0	41.0
48-49	37.45696265278137	40.0	38.0	41.0	33.0	41.0
50-51	37.284343650258506	40.0	37.0	41.0	33.0	41.0
52-53	37.15415200589642	40.0	37.0	41.0	32.5	41.0
54-55	36.99496856435027	40.0	36.5	41.0	32.0	41.0
56-57	36.814465052800415	40.0	36.0	41.0	32.0	41.0
58-59	36.61360407640665	40.0	35.5	41.0	31.5	41.0
60-61	36.404671892773536	39.0	35.0	41.0	31.0	41.0
62-63	36.17444215115304	39.0	35.0	41.0	31.0	41.0
64-65	35.89690221667599	39.0	35.0	41.0	31.0	41.0
66-67	35.6137191257978	38.0	35.0	41.0	30.0	41.0
68-69	35.34565458557867	37.0	35.0	40.0	30.0	41.0
70-71	35.098155746354514	37.0	35.0	40.0	30.0	41.0
72-73	34.84343433796814	36.0	35.0	39.0	30.0	41.0
74-75	34.54601272145384	36.0	35.0	39.0	30.0	41.0
76-77	34.23651091886907	35.5	35.0	39.0	30.0	40.5
78-79	33.93004445040917	35.0	35.0	37.0	30.0	39.5
80-81	33.63433948337087	35.0	35.0	37.0	29.0	39.0
82-83	33.32455628058198	35.0	34.0	36.5	29.0	39.0
84-85	33.06902465606942	35.0	34.0	36.0	29.0	38.0
86-87	32.850314031804714	35.0	34.0	36.0	29.0	37.0
88-89	32.63466017137273	35.0	34.0	35.5	29.0	37.0
90-91	32.44031837186171	35.0	34.0	35.0	29.0	36.5
92-93	32.29380259342757	35.0	34.0	35.0	29.0	36.0
94-95	32.16412179866681	35.0	34.0	35.0	29.0	36.0
96-97	32.03540176914144	35.0	34.0	35.0	27.5	36.0
98-99	31.917957337564438	35.0	34.0	35.0	27.0	36.0
100	31.8147245903733	35.0	34.0	35.0	27.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.21646025456124107
1101	2	0.19593239460209588
1101	3	0.22204675040514132
1101	4	0.12011290980980505
1101	5	0.1827051607267265
1101	6	0.20645600779790385
1101	7	0.18033949657308312
1101	8	0.21477253541159058
1101	9	0.26920251322394506
1101	10-11	0.2740011624657299
1101	12-13	0.27410260201843073
1101	14-15	0.3331490142578133
1101	16-17	0.35190253403757765
1101	18-19	0.32975062750217177
1101	20-21	0.3462354678354913
1101	22-23	0.34426311816130095
1101	24-25	0.34383724403003413
1101	26-27	0.3005782670605157
1101	28-29	0.37181845279368275
1101	30-31	0.37093181846072554
1101	32-33	0.3690523728195032
1101	34-35	0.39876030190296774
1101	36-37	0.40684274276329546
1101	38-39	0.4226700872708946
1101	40-41	0.4173331883104865
1101	42-43	0.34216748894439064
1101	44-45	0.38084147248748934
1101	46-47	0.47213715496634023
1101	48-49	0.4700529565688427
1101	50-51	0.43519579354244087
1101	52-53	0.45618513153220874
1101	54-55	0.4671722256188886
1101	56-57	0.47108054371113184
1101	58-59	0.42736565163668416
1101	60-61	0.34993934573870433
1101	62-63	0.3823341055188081
1101	64-65	0.4103278313599361
1101	66-67	0.46003625760055655
1101	68-69	0.4611313357635254
1101	70-71	0.4211583420491891
1101	72-73	0.3940940919508691
1101	74-75	0.37687279724761424
1101	76-77	0.3633615384354343
1101	78-79	0.3597288818638731
1101	80-81	0.35371329277979413
1101	82-83	0.3734108060611092
1101	84-85	0.379027931847574
1101	86-87	0.39895603406250757
1101	88-89	0.4314211366798091
1101	90-91	0.3961810552249645
1101	92-93	0.46941859135711184
1101	94-95	0.48194906441329977
1101	96-97	0.49715366330408983
1101	98-99	0.5058415827380536
1101	100	0.5583411989750182
1102	1	0.12379401427590153
1102	2	0.12828916322064998
1102	3	0.1474086231122982
1102	4	0.08713636229447275
1102	5	0.10532958187309305
1102	6	0.11647993676697865
1102	7	0.160300939913256
1102	8	0.1828131879142063
1102	9	0.21805957015545374
1102	10-11	0.1920714852217884
1102	12-13	0.16695948941256233
1102	14-15	0.2630478788592967
1102	16-17	0.2844840269717963
1102	18-19	0.260957163090481
1102	20-21	0.2657655166262245
1102	22-23	0.2615480454373511
1102	24-25	0.27638858540118605
1102	26-27	0.31505820666912854
1102	28-29	0.30223393114889774
1102	30-31	0.2697488466765918
1102	32-33	0.3068442910512772
1102	34-35	0.35428126647754254
1102	36-37	0.27570429612091374
1102	38-39	0.2942853257811535
1102	40-41	0.3069079319743082
1102	42-43	0.3764952071684178
1102	44-45	0.35409796956739115
1102	46-47	0.3452806788555307
1102	48-49	0.3661288961234348
1102	50-51	0.33962069727385114
1102	52-53	0.2923322874541938
1102	54-55	0.3199709247395788
1102	56-57	0.2891593360618643
1102	58-59	0.3085778768625218
1102	60-61	0.28507603512811386
1102	62-63	0.3120744531572299
1102	64-65	0.33072371616985663
1102	66-67	0.369673914373827
1102	68-69	0.3493896551520166
1102	70-71	0.3177689717469434
1102	72-73	0.3061950356499352
1102	74-75	0.31391223575882066
1102	76-77	0.3289789969833379
1102	78-79	0.3007803574319041
1102	80-81	0.2597889066926484
1102	82-83	0.30848108199555924
1102	84-85	0.2521641694121115
1102	86-87	0.23695592897455242
1102	88-89	0.25483182136081695
1102	90-91	0.2406504790061632
1102	92-93	0.21023556123389397
1102	94-95	0.22130804193997733
1102	96-97	0.23113658197685538
1102	98-99	0.2464169157818965
1102	100	0.2315005830470831
1103	1	0.09151148906045847
1103	2	0.1286843640917965
1103	3	0.10698559471624947
1103	4	0.09509097001065214
1103	5	0.12130456468671014
1103	6	0.11978505789827665
1103	7	0.07678722354840062
1103	8	0.07488371307390906
1103	9	0.13888366121492624
1103	10-11	0.20146920491681897
1103	12-13	0.18743697253211167
1103	14-15	0.2299885182137018
1103	16-17	0.22065042388719291
1103	18-19	0.2096785912678243
1103	20-21	0.18937158461690728
1103	22-23	0.2401271249383754
1103	24-25	0.22699198441980428
1103	26-27	0.1992443181445509
1103	28-29	0.21577050239462636
1103	30-31	0.19561496442903348
1103	32-33	0.14124901702465564
1103	34-35	0.12581809124746712
1103	36-37	0.1287784152914
1103	38-39	0.17572552977267009
1103	40-41	0.15590285079979793
1103	42-43	0.20480282440568942
1103	44-45	0.12626639744443935
1103	46-47	0.10973329951863064
1103	48-49	0.1158825548038962
1103	50-51	0.10586583702619379
1103	52-53	0.0994486211699126
1103	54-55	0.07083168755720237
1103	56-57	0.0212193260316198
1103	58-59	-0.011781730873700269
1103	60-61	-0.023745251129142275
1103	62-63	-0.001700052351282011
1103	64-65	-5.40569622160092E-4
1103	66-67	-0.013601106487506343
1103	68-69	-0.017641872527839553
1103	70-71	-0.029790101488515575
1103	72-73	0.04053927281417913
1103	74-75	0.06580248718113069
1103	76-77	0.024296378529029994
1103	78-79	0.023112036844452177
1103	80-81	0.06676186942631546
1103	82-83	0.07072986300812545
1103	84-85	0.04177586791152521
1103	86-87	0.049389218991137795
1103	88-89	0.029828377880015466
1103	90-91	0.08211022421435388
1103	92-93	0.07540327807868863
1103	94-95	0.07314224552180804
1103	96-97	0.08149315525001555
1103	98-99	0.03228965752188273
1103	100	0.08067576558736178
1104	1	0.09147103056034922
1104	2	0.13504212179673658
1104	3	0.14780517308325614
1104	4	0.03298089111473246
1104	5	0.05764819517018083
1104	6	0.0977147613467082
1104	7	0.08908563768604694
1104	8	0.13594177859598489
1104	9	0.17565914462010568
1104	10-11	0.15212544548342777
1104	12-13	0.17406887906595614
1104	14-15	0.19381060593142507
1104	16-17	0.20415330881841953
1104	18-19	0.20206239408602755
1104	20-21	0.21629780179887348
1104	22-23	0.21688918960768433
1104	24-25	0.20502311123152595
1104	26-27	0.19156198332331087
1104	28-29	0.19751829204885496
1104	30-31	0.14516716365333338
1104	32-33	0.11943925289217106
1104	34-35	0.1556436957140761
1104	36-37	0.202989419116669
1104	38-39	0.21265229379098827
1104	40-41	0.1920554303340083
1104	42-43	0.18739874153338576
1104	44-45	0.18207501876285903
1104	46-47	0.21991311076401843
1104	48-49	0.182755328893613
1104	50-51	0.1394573380808808
1104	52-53	0.16158287817634687
1104	54-55	0.1809003533910527
1104	56-57	0.16087758841479172
1104	58-59	0.1201417761429795
1104	60-61	0.12427642058652566
1104	62-63	0.14891632088964712
1104	64-65	0.1577671677057424
1104	66-67	0.1930961054230309
1104	68-69	0.18343077652848194
1104	70-71	0.16678160870890224
1104	72-73	0.14831300412613757
1104	74-75	0.15934962890817417
1104	76-77	0.15122910324828354
1104	78-79	0.1491588081637758
1104	80-81	0.163405933831811
1104	82-83	0.17782279692184488
1104	84-85	0.09835417062757301
1104	86-87	0.10651680588730983
1104	88-89	0.1226050530558922
1104	90-91	0.13944386772952555
1104	92-93	0.1485962379671264
1104	94-95	0.1592020014332718
1104	96-97	0.14252782781512963
1104	98-99	0.12881897404664855
1104	100	0.09917513764927222
1105	1	-0.023044142156699365
1105	2	-0.005340391056002147
1105	3	0.05327818887660385
1105	4	-0.08031614565651068
1105	5	-0.09618081933171396
1105	6	-0.12118002631615354
1105	7	-0.4411930793843055
1105	8	-0.4075953215044663
1105	9	-0.29254679554599505
1105	10-11	-0.37564553053852023
1105	12-13	-0.19593453029139596
1105	14-15	-0.13345576928404057
1105	16-17	-0.13763130107469124
1105	18-19	-0.13240515999363112
1105	20-21	-0.1639660315210989
1105	22-23	-0.13576634019951683
1105	24-25	-0.14975420744983836
1105	26-27	-0.20336309321634616
1105	28-29	-0.18849577686417973
1105	30-31	-0.18850229086447
1105	32-33	-0.22068459451843836
1105	34-35	-0.26288237851908036
1105	36-37	-0.22285115929610555
1105	38-39	-0.2822240957023894
1105	40-41	-0.3042595890415569
1105	42-43	-0.28843939292919174
1105	44-45	-0.2789325516772152
1105	46-47	-0.24308574067301691
1105	48-49	-0.20817239085777572
1105	50-51	-0.22866856037513372
1105	52-53	-0.20952803390212438
1105	54-55	-0.2392602412922571
1105	56-57	-0.21382651569861366
1105	58-59	-0.17974100320333974
1105	60-61	-0.18698922069476964
1105	62-63	-0.19536132889546565
1105	64-65	-0.11891765504513074
1105	66-67	-0.1548609067101978
1105	68-69	-0.21041811918058073
1105	70-71	-0.20663811061699278
1105	72-73	-0.1748314524329757
1105	74-75	-0.2486757698208919
1105	76-77	-0.22082680290851897
1105	78-79	-0.17353646720292915
1105	80-81	-0.1968497426873128
1105	82-83	-0.18597433667102337
1105	84-85	-0.17081304725442692
1105	86-87	-0.1989836411402024
1105	88-89	-0.21446776598541106
1105	90-91	-0.15666581769194465
1105	92-93	-0.13611646960008983
1105	94-95	-0.22144368740111275
1105	96-97	-0.2149304152824456
1105	98-99	-0.18206883815160424
1105	100	-0.22912284673791206
1106	1	0.05654556739398231
1106	2	0.1006623463733689
1106	3	0.09794309718134286
1106	4	0.05902209207086173
1106	5	0.07730873081860778
1106	6	0.08717027407478639
1106	7	0.05829470851429619
1106	8	0.08900054826376902
1106	9	0.08831931425284267
1106	10-11	0.11372782638555634
1106	12-13	0.1075360740853526
1106	14-15	0.13589978267974345
1106	16-17	0.1578707116334499
1106	18-19	0.15966465934683782
1106	20-21	0.14993023467540212
1106	22-23	0.1732084487848624
1106	24-25	0.16161787873015498
1106	26-27	0.16101382330643332
1106	28-29	0.17138653126409764
1106	30-31	0.17165288717381344
1106	32-33	0.14015815212103178
1106	34-35	0.1693439617101049
1106	36-37	0.15849348248677586
1106	38-39	0.17488764939707835
1106	40-41	0.18276263661374514
1106	42-43	0.1427298988852641
1106	44-45	0.11766704968255937
1106	46-47	0.12172942014333188
1106	48-49	0.16401559148417988
1106	50-51	0.18596544843577334
1106	52-53	0.1799642680465965
1106	54-55	0.2320109234066976
1106	56-57	0.2187717536092606
1106	58-59	0.2359348794639331
1106	60-61	0.24125814132690948
1106	62-63	0.260041591116277
1106	64-65	0.2513401430667983
1106	66-67	0.2489164893547411
1106	68-69	0.25987168483988654
1106	70-71	0.2589702659482356
1106	72-73	0.20551067790607647
1106	74-75	0.2108965288290534
1106	76-77	0.18704252058746107
1106	78-79	0.1739971358442105
1106	80-81	0.1889079088605854
1106	82-83	0.1969715693593912
1106	84-85	0.1917296200332146
1106	86-87	0.168702602724224
1106	88-89	0.18397423794107226
1106	90-91	0.1956998262893137
1106	92-93	0.17941889926925825
1106	94-95	0.19566918457803695
1106	96-97	0.23870567015698185
1106	98-99	0.17563241568057109
1106	100	0.22884506349070577
1107	1	-0.024059563772269144
1107	2	-0.01123220694239535
1107	3	-3.7059875392486674E-4
1107	4	-0.09983827734938444
1107	5	-0.08910816574394431
1107	6	-0.025763590345050602
1107	7	-0.026633781292815684
1107	8	-0.0335970703992956
1107	9	0.01900200778974437
1107	10-11	0.03139974077159735
1107	12-13	0.06066799621937946
1107	14-15	0.024518932845666086
1107	16-17	0.08666803590885763
1107	18-19	0.0729992136475559
1107	20-21	0.047179595565779664
1107	22-23	0.04363980998529371
1107	24-25	0.04358570193490863
1107	26-27	0.06012308481140849
1107	28-29	0.07748535850816296
1107	30-31	0.11187660384579345
1107	32-33	0.09365604404919026
1107	34-35	0.10847108952428641
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2216	38-39	-0.5740167943788208
2216	40-41	-0.5423004003030343
2216	42-43	-0.5561423792060509
2216	44-45	-0.6375857547838706
2216	46-47	-0.5400410394047341
2216	48-49	-0.4836874173558954
2216	50-51	-0.3991903711640177
2216	52-53	-0.4792496439116576
2216	54-55	-0.5162742393721516
2216	56-57	-0.5076352744929267
2216	58-59	-0.6309929946490413
2216	60-61	-0.6227550838973102
2216	62-63	-0.6824563706415248
2216	64-65	-0.5780436593133658
2216	66-67	-0.4992361314909175
2216	68-69	-0.5399281120767654
2216	70-71	-0.5301425571882845
2216	72-73	-0.5745479129206075
2216	74-75	-0.4601116839093322
2216	76-77	-0.3865083046071902
2216	78-79	-0.4088386505110435
2216	80-81	-0.42574903795332375
2216	82-83	-0.45139241766045046
2216	84-85	-0.43888519406262105
2216	86-87	-0.42606360585892133
2216	88-89	-0.37060225470403196
2216	90-91	-0.3563207514243629
2216	92-93	-0.5757157728913249
2216	94-95	-0.5279487856507217
2216	96-97	-0.589889248830282
2216	98-99	-0.5952156678889402
2216	100	-0.6253979515860415
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	68.0
3	476.0
4	271.0
5	427.0
6	573.0
7	916.0
8	1785.0
9	2807.0
10	3486.0
11	4774.0
12	7274.0
13	10742.0
14	11683.0
15	15446.0
16	21165.0
17	33763.0
18	41666.0
19	22375.0
20	13977.0
21	12889.0
22	12524.0
23	12605.0
24	13479.0
25	15928.0
26	19470.0
27	24640.0
28	30121.0
29	37809.0
30	48218.0
31	60621.0
32	75253.0
33	97610.0
34	134368.0
35	226500.0
36	403037.0
37	888410.0
38	1467440.0
39	833893.0
40	11266.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.62487820264007	21.379768984963913	15.174376151811735	23.820976660584286
2	13.178750821201557	22.16898948104391	40.67633456752577	23.975925130228767
3	15.187119959426262	28.997126892721383	31.8264437997228	23.98930934812956
4	10.428171488526873	18.707270574793906	39.57461012494315	31.28994781173607
5	11.875391660380258	38.80567259519174	34.990621797043346	14.328313947384656
6	27.65194258137066	39.82215507099403	18.63237336179083	13.893528985844489
7	24.41910880555354	34.72257727953106	23.587917541081723	17.270396373833677
8	25.067779568397025	34.9351210183224	21.03570427436087	18.961395138919706
9	26.103375611910156	15.805232095641436	21.941098608043067	36.15029368440534
10-11	23.282890542896755	27.37970953005084	29.044332437542685	20.293067489509724
12-13	24.74646382762722	25.671696875700118	29.339218205294436	20.242621091378222
14-15	22.110230953805992	26.33607193455064	28.076066804408462	23.477630307234907
16-17	20.207045611726162	29.65292531746813	28.299119758515335	21.84090931229037
18-19	20.148665892455337	27.503244219661	32.091723478842496	20.25636640904117
20-21	21.828148895341855	26.490712169801213	31.713770535450475	19.967368399406464
22-23	21.314724822771794	26.782672222522862	31.640640727312086	20.26196222739326
24-25	20.861576645410473	26.73288812179616	31.810866883207893	20.594668349585472
26-27	20.803663502558155	26.885412400546176	31.725844702679723	20.585079394215942
28-29	20.557993416908495	26.813997576473348	32.05726588269317	20.570743123924988
30-31	20.982603329193793	26.84703930599477	31.689877885401945	20.480479479409492
32-33	20.601710920335385	26.904388415304876	31.948223213009115	20.54567745135062
34-35	20.965883932049493	26.65672621736139	31.667388063602857	20.71000178698626
36-37	21.007171118106708	26.943467171475284	31.379462060612752	20.669899649805256
38-39	20.83971820345503	26.87166438113751	31.455317454046792	20.83329996136067
40-41	20.911306370964233	26.822544554191825	31.365752741742302	20.90039633310164
42-43	20.909582771387136	27.073195888897057	31.206107451869247	20.81111388784656
44-45	21.168692576522247	26.96893869453551	30.905344137704873	20.957024591237374
46-47	21.146361529037645	27.18505731612823	30.52959039622166	21.138990758612465
48-49	21.245289145281568	27.291309947723313	29.97827757514097	21.485123331854144
50-51	21.463354816267284	27.4162239714649	29.54229737406876	21.578123838199055
52-53	21.248401836071594	27.66047416588912	29.627050528334355	21.46407346970494
54-55	21.46666500711892	27.670768816159807	29.474325623832115	21.388240552889158
56-57	21.205406910118757	27.578999410786547	29.58441402700338	21.63117965209132
58-59	21.290119174958743	27.314141623935907	29.808762240053266	21.58697696105208
60-61	21.517830500579198	27.55539085166834	29.451602076611046	21.475176571141418
62-63	21.21088672451245	27.383032217076448	29.928286240287633	21.47779481812347
64-65	21.342170742820777	27.210425661101073	29.904161584326445	21.54324201175171
66-67	21.279234065719386	27.359757387762347	29.752760856048017	21.60824769047025
68-69	21.446442771016855	27.242446062553704	29.664212805671504	21.646898360757937
70-71	21.484705702558824	27.312847279119712	29.500593441556234	21.70185357676523
72-73	21.419977117811882	27.497469830892385	29.489720278183857	21.59283277311188
74-75	21.606598580133756	27.38440411367892	29.230535447355926	21.778461858831403
76-77	21.53313506833605	27.372475048936707	29.234234458496932	21.86015542423031
78-79	21.516548560330776	27.50840042452909	29.273512367775705	21.701538647364433
80-81	21.30561634870177	27.53502188499148	29.309112665631034	21.850249100675722
82-83	21.3380785198343	27.53055989145613	29.510320910518196	21.62104067819137
84-85	21.400460462601266	27.335724252343553	29.168699626079775	22.095115658975406
86-87	21.491680219908314	27.49629930752649	29.1107828822098	21.901237590355397
88-89	21.208142194884154	27.490687682804555	29.162164198101692	22.139005924209602
90-91	21.31902773415405	27.351205699611857	29.045391209743183	22.28437535649091
92-93	21.211634159126273	27.491446537653907	29.256656472106314	22.04026283111351
94-95	21.110273035507312	27.286893843239746	29.270438050176523	22.332395071076423
96-97	21.203351033449618	27.560370110329803	28.93279024739864	22.303488608821947
98-99	20.997907744419468	27.400820255969304	29.161557609652927	22.439714389958297
100	21.154267018817833	27.377696847372512	29.039180172685686	22.42885596112397
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2252.0
1	1542.5
2	2815.5
3	4092.0
4	2174.0
5	1385.0
6	3444.5
7	9642.5
8	16845.5
9	17352.0
10	10856.5
11	5705.0
12	4941.0
13	5288.0
14	5874.5
15	6479.0
16	7186.5
17	8062.5
18	9137.0
19	10520.5
20	13140.0
21	15886.5
22	18290.5
23	22320.5
24	29004.0
25	35502.5
26	42533.5
27	52092.0
28	63641.5
29	77375.5
30	87686.5
31	96436.5
32	106161.0
33	113572.5
34	120486.5
35	128342.0
36	133408.5
37	136284.0
38	139602.0
39	142431.5
40	144772.5
41	145379.5
42	143571.0
43	142728.5
44	142161.0
45	141720.5
46	143277.5
47	140945.0
48	135678.5
49	134023.0
50	135682.5
51	134213.5
52	128623.0
53	125473.0
54	124083.5
55	120323.0
56	109284.5
57	94814.5
58	87299.5
59	84236.0
60	74763.0
61	61159.5
62	51537.5
63	41966.5
64	32173.5
65	24719.0
66	18807.5
67	13941.5
68	10193.0
69	7308.0
70	5074.0
71	3708.5
72	2653.5
73	1629.5
74	1050.5
75	759.0
76	522.0
77	341.0
78	221.5
79	107.0
80	34.0
81	12.0
82	7.5
83	8.0
84	5.0
85	7.0
86	7.0
87	4.0
88	3.0
89	4.0
90	5.0
91	3.0
92	1.0
93	1.5
94	4.0
95	9.5
96	16.0
97	17.0
98	9.5
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008701760158276792
2	0.0
3	3.896310518631399E-4
4	0.0029005867194255974
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	1.0823084773976109E-4
24-25	7.576159341783276E-5
26-27	4.97861899602901E-4
28-29	8.87492951466041E-4
30-31	0.001688401224740273
32-33	0.0042101799770767065
34-35	0.0053141346240222695
36-37	0.00395042594250128
38-39	0.0023810786502747442
40-41	0.003376802449480546
42-43	4.653926452809727E-4
44-45	4.3292339095904437E-4
46-47	0.003387625534254522
48-49	0.002272847802534983
50-51	8.983160362400171E-4
52-53	1.082308477397611E-5
54-55	4.870388148289249E-4
56-57	8.00908273274232E-4
58-59	7.467928494043515E-4
60-61	3.571617975412116E-4
62-63	0.0
64-65	0.0
66-67	8.117313580482082E-4
68-69	6.169158321166382E-4
70-71	0.001991447598411604
72-73	9.740776296578498E-5
74-75	0.0020455630222814846
76-77	2.597540345754266E-4
78-79	0.0010390161383017065
80-81	7.900851885002559E-4
82-83	0.0012121854946853241
84-85	0.0011472469860414676
86-87	2.489309498014505E-4
88-89	0.0014502933597127987
90-91	0.0015585242074525596
92-93	0.0029114098041995735
94-95	9.632545448838737E-4
96-97	0.002283670887308959
98-99	0.002153793870021246
100	0.003203633093096928
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4619755.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.58386537829739
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.47262360443591	28.97133878517968
2	16.66817984620589	14.529274130365746
3	6.6057579499277	8.637133956338
4	3.286251209804958	5.729101213096248
5	1.8817041078972023	4.100596926019043
6	1.1728227902764277	3.0669690362404167
7	0.7977081421204235	2.4337042994143783
8	0.5453955695511141	1.9016357664988475
9	0.40552989958777924	1.5907104495457407
>10	1.953462935220186	14.965104587664063
>50	0.13000252860127093	3.8654184337772457
>100	0.07454558964334705	6.0283352122132685
>500	0.00427571201626843	1.256662830840901
>1k	0.001491526895575889	1.0019886366112754
>5k	9.943512637167767E-5	0.2375771158020471
>10k+	1.4915268955751653E-4	1.684448620392916
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34373	0.7440437858717617	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24077	0.5211748242060456	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19286	0.4174680259018065	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5865	0.12695478439873975	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5099	0.11037381852500837	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	2.164616954795222E-5	0.0
18-19	0.0	0.0	0.0	4.329233909590444E-5	0.0
20-21	0.0	0.0	0.0	8.658467819180887E-5	0.0
22-23	0.0	0.0	0.0	2.489309498014505E-4	0.0
24-25	0.0	0.0	0.0	4.5456956050699655E-4	0.0
26-27	0.0	0.0	0.0	7.035005103084471E-4	0.0
28-29	0.0	0.0	0.0	0.002251201632987031	0.0
30-31	0.0	0.0	0.0	0.006190804490714335	0.0
32-33	0.0	0.0	0.0	0.013388155865408447	0.0
34-35	0.0	0.0	0.0	0.023724201824555632	0.0
36-37	0.0	0.0	0.0	0.03928779772953328	0.0
38-39	0.0	0.0	0.0	0.06122619056638284	0.0
40-41	0.0	0.0	0.0	0.08569718524034284	0.0
42-43	0.0	0.0	0.0	0.10843648635046664	0.0
44-45	0.0	0.0	0.0	0.13761552290110624	0.0
46-47	0.0	0.0	0.0	0.17109132410701433	0.0
48-49	0.0	0.0	0.0	0.20786816616898515	0.0
50-51	0.0	0.0	0.0	0.24676633284665528	0.0
52-53	0.0	0.0	0.0	0.2852423992181403	0.0
54-55	0.0	0.0	0.0	0.3314353250334704	0.0
56-57	0.0	0.0	0.0	0.38335366269423377	0.0
58-59	0.0	0.0	0.0	0.4434434293593491	0.0
60-61	0.0	0.0	0.0	0.5090962615982882	0.0
62-63	0.0	0.0	0.0	0.5764807874010635	0.0
64-65	0.0	0.0	0.0	0.6468200153471342	0.0
66-67	0.0	0.0	0.0	0.7295408522746336	0.0
68-69	0.0	0.0	0.0	0.8268187382231309	0.0
70-71	0.0	0.0	0.0	0.9360561328468717	0.0
72-73	0.0	0.0	0.0	1.0531294408469711	0.0
74-75	0.0	0.0	0.0	1.17158810369814	0.0
76-77	0.0	0.0	0.0	1.303737968788388	0.0
78-79	0.0	0.0	0.0	1.4551853940306358	0.0
80-81	0.0	0.0	0.0	1.6319263683896656	0.0
82-83	0.0	0.0	0.0	1.8231702763458235	0.0
84-85	0.0	0.0	0.0	2.0175961712255304	0.0
86-87	0.0	0.0	0.0	2.223418774372234	0.0
88	0.0	0.0	0.0	2.3926377048133505	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10440	0.0	63.885727	1
GTATCAA	22740	0.0	49.751656	1
TATCAAC	30575	0.0	36.784927	2
ATCAACG	30805	0.0	36.388226	3
TCAACGC	31015	0.0	36.156998	4
CAACGCA	32510	0.0	34.464626	5
AACGCAG	33475	0.0	33.44302	6
ACGCAGA	37315	0.0	29.850334	7
GTGGTAT	3315	0.0	29.35004	1
CGCAGAG	37795	0.0	29.32201	8
GCAGAGT	40485	0.0	27.013853	9
GTACATG	24045	0.0	25.236168	1
TACATGG	24500	0.0	24.401402	2
TGGTATC	3820	0.0	24.361025	2
ACATGGG	24920	0.0	23.122572	3
AGAGTAC	38270	0.0	23.044876	10-11
AGTACTT	27105	0.0	22.532593	12-13
GAGTACT	26250	0.0	22.344446	12-13
CAGAGTA	39715	0.0	21.650211	10-11
GTACTTT	29135	0.0	20.164122	14-15
>>END_MODULE