FastQCFastQC Report
Wed 25 May 2016
SRR2431366_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431366_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6228201
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA288820.46372941399932344No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67250.044.937921
GTATCAA122600.040.0991821
TCAACGC135150.034.8106654
ATCAACG136750.034.4033743
CAACGCA143900.032.7266245
AACGCAG151300.031.1570476
TATCAAC157200.030.8248122
ACGCAGA174300.026.8029927
CGCAGAG174550.026.387648
GTACATG259750.025.8565731
TACATGG261600.024.631682
ACATGGG262200.023.5894323
GCAGAGT200150.022.143719
CATGGGT109200.021.6921314
CATGGGG120800.019.7258324
AGAGTAC176550.018.28872110-11
CAGAGTA191650.017.90227910-11
ATGGGTG71900.016.4727445
GAGTACA189800.015.8728931
CTATGCG2851.7505665E-514.8419839
Produced by FastQC (version 0.11.5)