##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431366_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6228201
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.27012535401475	33.0	31.0	34.0	28.0	34.0
2	31.368840055097774	34.0	31.0	34.0	28.0	34.0
3	31.467257559606697	34.0	31.0	34.0	28.0	34.0
4	34.66376663180909	37.0	35.0	37.0	32.0	37.0
5	34.80962817352876	37.0	35.0	37.0	32.0	37.0
6	34.85095503500931	37.0	35.0	37.0	32.0	37.0
7	34.77407312320203	37.0	35.0	37.0	32.0	37.0
8	34.75316965525037	37.0	35.0	37.0	32.0	37.0
9	36.26141818480168	39.0	38.0	39.0	32.0	39.0
10-11	36.27582956940535	39.0	37.5	39.0	32.0	39.0
12-13	36.2252170410043	39.0	37.0	39.0	32.0	39.0
14-15	37.637694817492246	41.0	38.0	41.0	32.0	41.0
16-17	37.6594813654858	41.0	38.0	41.0	32.0	41.0
18-19	37.701211794545486	41.0	38.5	41.0	32.0	41.0
20-21	37.76162041013127	41.0	39.0	41.0	32.0	41.0
22-23	37.768779218910886	41.0	39.0	41.0	32.0	41.0
24-25	37.7984927750405	41.0	38.5	41.0	32.0	41.0
26-27	37.80450550648574	41.0	38.0	41.0	32.0	41.0
28-29	37.7755944292742	41.0	38.0	41.0	31.0	41.0
30-31	37.712976507983605	41.0	38.0	41.0	31.0	41.0
32-33	37.641549863275124	41.0	38.0	41.0	31.0	41.0
34-35	37.57590827592109	40.0	38.0	41.0	30.5	41.0
36-37	37.48878697074805	40.0	38.0	41.0	30.0	41.0
38-39	37.39930390814298	40.0	38.0	41.0	30.0	41.0
40-41	37.32169361586115	40.0	38.0	41.0	30.0	41.0
42-43	37.21769584828749	40.0	38.0	41.0	30.0	41.0
44-45	37.111729454460445	40.0	38.0	41.0	30.0	41.0
46-47	37.00811068878477	40.0	37.5	41.0	30.0	41.0
48-49	36.90058445448372	40.0	37.0	41.0	30.0	41.0
50-51	36.78920213075975	40.0	37.0	41.0	30.0	41.0
52-53	36.64731838294878	40.0	37.0	41.0	30.0	41.0
54-55	36.504311678444544	40.0	36.0	41.0	30.0	41.0
56-57	36.332586488457906	40.0	36.0	41.0	29.5	41.0
58-59	36.13529765657852	39.5	35.0	41.0	29.0	41.0
60-61	35.979242641655276	39.0	35.0	41.0	29.0	41.0
62-63	35.83826557620732	39.0	35.0	41.0	29.0	41.0
64-65	35.60840401586269	39.0	35.0	41.0	28.5	41.0
66-67	35.332195123439334	38.5	35.0	40.5	28.0	41.0
68-69	35.04052606844256	38.0	35.0	40.0	28.0	41.0
70-71	34.795140282081455	37.0	34.0	40.0	28.0	41.0
72-73	34.55087849605368	37.0	34.0	39.5	28.0	41.0
74-75	34.19931542029552	36.0	34.0	39.0	28.0	41.0
76-77	33.83405521112758	36.0	34.0	39.0	27.0	40.5
78-79	33.47374691343455	35.5	34.0	38.5	27.0	40.0
80-81	33.10946595975307	35.0	34.0	37.0	26.5	39.0
82-83	32.7520110542354	35.0	34.0	37.0	26.0	39.0
84-85	32.43131716526169	35.0	33.0	36.5	26.0	38.5
86-87	32.143258623156186	35.0	33.0	36.0	25.5	37.0
88-89	31.870917059356305	35.0	33.0	36.0	25.0	37.0
90-91	31.644373632129085	35.0	33.0	35.0	25.0	36.5
92-93	31.42714083890356	35.0	33.0	35.0	24.5	36.0
94-95	31.213136345471188	35.0	33.0	35.0	24.0	36.0
96-97	31.025729660940613	35.0	32.5	35.0	23.0	36.0
98-99	30.9051581668607	35.0	32.5	35.0	23.0	36.0
100	30.861185597574647	35.0	33.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.34791211530664157
1101	2	0.3845327218603707
1101	3	0.35348992738439833
1101	4	0.383895943603342
1101	5	0.289743923199822
1101	6	0.3136713480515212
1101	7	0.33027378762301396
1101	8	0.33717475666627195
1101	9	0.4375556250330348
1101	10-11	0.36588883064989375
1101	12-13	0.3433757595437257
1101	14-15	0.40438856535722323
1101	16-17	0.3993926870703248
1101	18-19	0.3847218142023294
1101	20-21	0.367898201886824
1101	22-23	0.41111490349299373
1101	24-25	0.4660202305058121
1101	26-27	0.46271968069969205
1101	28-29	0.4654472902313387
1101	30-31	0.4957454525455347
1101	32-33	0.4661098729215354
1101	34-35	0.49720915790412334
1101	36-37	0.5029250541858303
1101	38-39	0.4477344308120266
1101	40-41	0.4705818087602225
1101	42-43	0.4550648917633069
1101	44-45	0.4393941279166782
1101	46-47	0.456121391002128
1101	48-49	0.43747251667024756
1101	50-51	0.4365221313911576
1101	52-53	0.4301102945863633
1101	54-55	0.4940712105033782
1101	56-57	0.4337563586769946
1101	58-59	0.47536145388964
1101	60-61	0.5162875308400316
1101	62-63	0.4747983683641408
1101	64-65	0.4599377389742898
1101	66-67	0.4328575398756698
1101	68-69	0.470520783083046
1101	70-71	0.4512684402519582
1101	72-73	0.5044757856445656
1101	74-75	0.49321125542356015
1101	76-77	0.4573410523187462
1101	78-79	0.42213405004360993
1101	80-81	0.46753180379494097
1101	82-83	0.46778648618780494
1101	84-85	0.4990787889039652
1101	86-87	0.43638073848435965
1101	88-89	0.5037407748509288
1101	90-91	0.47815396559617795
1101	92-93	0.45188816451417324
1101	94-95	0.537294811983287
1101	96-97	0.47381449186657676
1101	98-99	0.4786106694420731
1101	100	0.5127720227841124
1102	1	0.17824644393703437
1102	2	0.25339136537664686
1102	3	0.29892075437876997
1102	4	0.23337284538617808
1102	5	0.18903966403992456
1102	6	0.1781894770667236
1102	7	0.22320809591606405
1102	8	0.27046116670148734
1102	9	0.2914859156510943
1102	10-11	0.31368332183613035
1102	12-13	0.3050192198285515
1102	14-15	0.4045377207273475
1102	16-17	0.3618412239374251
1102	18-19	0.33195701542643263
1102	20-21	0.3537585380454047
1102	22-23	0.2761414791220531
1102	24-25	0.3425704930423734
1102	26-27	0.3520605907073815
1102	28-29	0.38261520333039556
1102	30-31	0.3587928405420513
1102	32-33	0.3426431832983283
1102	34-35	0.36210087785475764
1102	36-37	0.39773041607912063
1102	38-39	0.37838135936956263
1102	40-41	0.4017771285249623
1102	42-43	0.3113200181174349
1102	44-45	0.3339342823964202
1102	46-47	0.2753324240927384
1102	48-49	0.23591555205595682
1102	50-51	0.23340677613271765
1102	52-53	0.21400371537305318
1102	54-55	0.27882635513589094
1102	56-57	0.30916067622269594
1102	58-59	0.26276775348554793
1102	60-61	0.2935186740506168
1102	62-63	0.2987373601279373
1102	64-65	0.3134968416354127
1102	66-67	0.32461332509236485
1102	68-69	0.26162220705396066
1102	70-71	0.25879976353623135
1102	72-73	0.28885850800332236
1102	74-75	0.2812342932379792
1102	76-77	0.2900248111044519
1102	78-79	0.2761162082302846
1102	80-81	0.2653059970349645
1102	82-83	0.3238463672563938
1102	84-85	0.3082720935283163
1102	86-87	0.3282178966941487
1102	88-89	0.3019766025827444
1102	90-91	0.2991622821603066
1102	92-93	0.32015656823053007
1102	94-95	0.2839293421172151
1102	96-97	0.31937988303050346
1102	98-99	0.32524308810637237
1102	100	0.27310795550113554
1103	1	0.15963592742300392
1103	2	0.23118975571887646
1103	3	0.25033760877872524
1103	4	0.20294433670493106
1103	5	0.16711452053962006
1103	6	0.20364953688623189
1103	7	0.2244234250271404
1103	8	0.23026005418788742
1103	9	0.290651477329817
1103	10-11	0.2629095586312502
1103	12-13	0.266615824001704
1103	14-15	0.25873644085449143
1103	16-17	0.28909853921329187
1103	18-19	0.2813614801935742
1103	20-21	0.25454393695280686
1103	22-23	0.29556547129543986
1103	24-25	0.2865736390481928
1103	26-27	0.28882449907727903
1103	28-29	0.3094749535606667
1103	30-31	0.27485737050798775
1103	32-33	0.20648454004948036
1103	34-35	0.2852509171098774
1103	36-37	0.34928481689459545
1103	38-39	0.29354319928183514
1103	40-41	0.2729658853243251
1103	42-43	0.24959124293571477
1103	44-45	0.2264863431932156
1103	46-47	0.22367603953833282
1103	48-49	0.2743950314626247
1103	50-51	0.2654767337956514
1103	52-53	0.20856566660768294
1103	54-55	0.2123567988159465
1103	56-57	0.14949185052277159
1103	58-59	0.14436773034110928
1103	60-61	0.16659891593330656
1103	62-63	0.1657979495021138
1103	64-65	0.20092097279980692
1103	66-67	0.1605014839272556
1103	68-69	0.1798260686561406
1103	70-71	0.18810448179092987
1103	72-73	0.1448255025123828
1103	74-75	0.13144718604205252
1103	76-77	0.1670251744140714
1103	78-79	0.13306708100126485
1103	80-81	0.09582205268495159
1103	82-83	0.14951960102321493
1103	84-85	0.13726009679641749
1103	86-87	0.1487318928727248
1103	88-89	0.20770148741572214
1103	90-91	0.182072315260843
1103	92-93	0.1793168900211235
1103	94-95	0.14927065636003078
1103	96-97	0.09206805187772815
1103	98-99	0.07938772249280035
1103	100	0.07672621459637341
1104	1	0.21688623325070466
1104	2	0.2131711943648149
1104	3	0.20036317162520234
1104	4	0.1513100356292867
1104	5	0.19140080522880965
1104	6	0.1685770291488211
1104	7	0.20253429675037893
1104	8	0.15217734351436718
1104	9	0.19745619473732745
1104	10-11	0.18014138604248586
1104	12-13	0.22219217726108553
1104	14-15	0.26724627802430234
1104	16-17	0.26727040842742866
1104	18-19	0.20731273150362028
1104	20-21	0.22329992136328514
1104	22-23	0.2292504472923298
1104	24-25	0.26807583132825386
1104	26-27	0.2818548485525554
1104	28-29	0.3158139697436084
1104	30-31	0.349997095931073
1104	32-33	0.31433638771433436
1104	34-35	0.3087369814384431
1104	36-37	0.27284519645791505
1104	38-39	0.27564183507332274
1104	40-41	0.2723562665047936
1104	42-43	0.2752140311285203
1104	44-45	0.2767524363135436
1104	46-47	0.3329186349933124
1104	48-49	0.27191838589882167
1104	50-51	0.23504586398754412
1104	52-53	0.25984434056671546
1104	54-55	0.21100726368941025
1104	56-57	0.2523339637652242
1104	58-59	0.21825435214825717
1104	60-61	0.23307490484325655
1104	62-63	0.2008646036294408
1104	64-65	0.20735470251812416
1104	66-67	0.23152896392161892
1104	68-69	0.21057187143340172
1104	70-71	0.21782927003299335
1104	72-73	0.20816358782762734
1104	74-75	0.25553767903085145
1104	76-77	0.22304964557788054
1104	78-79	0.16492580061694895
1104	80-81	0.19652098872353463
1104	82-83	0.17847679169219077
1104	84-85	0.19567176636358852
1104	86-87	0.21155662845229273
1104	88-89	0.21488711553725537
1104	90-91	0.2348187147939811
1104	92-93	0.21516089357356805
1104	94-95	0.18359923709922654
1104	96-97	0.23938284599701376
1104	98-99	0.23706579915120685
1104	100	0.21053436879143206
1105	1	0.11257575121039665
1105	2	0.16020296780744658
1105	3	0.17072061301751518
1105	4	0.08553496638496938
1105	5	0.08926774852255193
1105	6	0.11229446647140406
1105	7	0.16534956092897346
1105	8	0.10229680895354676
1105	9	0.15409517120809113
1105	10-11	0.14855800591367085
1105	12-13	0.1577761443526171
1105	14-15	0.17905987420908787
1105	16-17	0.14272452978644878
1105	18-19	0.17545019765997694
1105	20-21	0.17201512707530497
1105	22-23	0.19409148217673788
1105	24-25	0.21609882921134016
1105	26-27	0.20426457526431818
1105	28-29	0.24699004940877245
1105	30-31	0.2532562226120376
1105	32-33	0.26612292442079166
1105	34-35	0.23278309077142723
1105	36-37	0.23103690545514155
1105	38-39	0.25758192458551576
1105	40-41	0.26800194300361113
1105	42-43	0.27808733563260546
1105	44-45	0.22559052840155402
1105	46-47	0.20969956019612113
1105	48-49	0.21004920937535587
1105	50-51	0.18789083891756064
1105	52-53	0.26747436638321176
1105	54-55	0.24068856906617953
1105	56-57	0.23455699129768703
1105	58-59	0.266663224924919
1105	60-61	0.30520832265885645
1105	62-63	0.36245871951270203
1105	64-65	0.36062462670737716
1105	66-67	0.36747851751101734
1105	68-69	0.3355769124671397
1105	70-71	0.3502293710532385
1105	72-73	0.36110584011743896
1105	74-75	0.33294459236569196
1105	76-77	0.30212865709320624
1105	78-79	0.3075805409603163
1105	80-81	0.27971171398962724
1105	82-83	0.36213118662825394
1105	84-85	0.4139002347105887
1105	86-87	0.35325627411704374
1105	88-89	0.3749530686383018
1105	90-91	0.3726308809255201
1105	92-93	0.3450019945421161
1105	94-95	0.4121970977127489
1105	96-97	0.4210817717023616
1105	98-99	0.4027294366037495
1105	100	0.38392574621572706
1106	1	0.08617281118302955
1106	2	0.12178594550340804
1106	3	0.07723747896306676
1106	4	0.055750416410454307
1106	5	0.0929737231972112
1106	6	0.07142452266867849
1106	7	0.07830375162728842
1106	8	0.020052727040848595
1106	9	0.11624236154115408
1106	10-11	0.08097608522366073
1106	12-13	0.08996408023885749
1106	14-15	0.1257453153780972
1106	16-17	0.13853216224828913
1106	18-19	0.07086888666793101
1106	20-21	0.11736765474270072
1106	22-23	0.11875106233837585
1106	24-25	0.12874696083206771
1106	26-27	0.1929154519495384
1106	28-29	0.2069672016323878
1106	30-31	0.1903322534384131
1106	32-33	0.19325399866371384
1106	34-35	0.17375366938039605
1106	36-37	0.21862477316796003
1106	38-39	0.20939686867747298
1106	40-41	0.20586384736350993
1106	42-43	0.19692403447687923
1106	44-45	0.18530070642357543
1106	46-47	0.18503183680822843
1106	48-49	0.1734011558650579
1106	50-51	0.1458490606552445
1106	52-53	0.12821893938084372
1106	54-55	0.12004208089656743
1106	56-57	0.16573554202075513
1106	58-59	0.14738974354526846
1106	60-61	0.1251778137959576
1106	62-63	0.18308854871043678
1106	64-65	0.2284922626457444
1106	66-67	0.21622632919692109
1106	68-69	0.19204816614292497
1106	70-71	0.1702443949734942
1106	72-73	0.11640193869110504
1106	74-75	0.13403486050324886
1106	76-77	0.173670662877079
1106	78-79	0.18948700879369795
1106	80-81	0.12993416856586748
1106	82-83	0.14690018830810203
1106	84-85	0.11667724569952753
1106	86-87	0.13198552057581736
1106	88-89	0.14861792002038854
1106	90-91	0.1257211664789466
1106	92-93	0.19648631700457742
1106	94-95	0.2239583318651981
1106	96-97	0.1759423251219019
1106	98-99	0.19471805967198108
1106	100	0.2031199284215539
1107	1	0.05157504216123954
1107	2	0.11596521725445896
1107	3	0.14940367071999106
1107	4	0.11481035683804919
1107	5	0.12507786553808842
1107	6	0.14640265653977735
1107	7	0.18995834947109103
1107	8	0.15765513118061847
1107	9	0.11439518031099283
1107	10-11	0.15681616052489034
1107	12-13	0.14788499780306097
1107	14-15	0.152477374934179
1107	16-17	0.1911113648808751
1107	18-19	0.16954629921382747
1107	20-21	0.16965014677355583
1107	22-23	0.16009559324840694
1107	24-25	0.14627950467627926
1107	26-27	0.21607880546466163
1107	28-29	0.1647964505549666
1107	30-31	0.15762113642061593
1107	32-33	0.2331123529695347
1107	34-35	0.22871436388247446
1107	36-37	0.17510240301129443
1107	38-39	0.18599723223346842
1107	40-41	0.17753825622174446
1107	42-43	0.17921215141165447
1107	44-45	0.19706767063501474
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2216	70-71	-0.9344633425491793
2216	72-73	-0.9259324161224711
2216	74-75	-0.9689602880993107
2216	76-77	-0.9121720393056663
2216	78-79	-0.8343501196048493
2216	80-81	-0.7632414626670965
2216	82-83	-0.7597993216095063
2216	84-85	-0.779831040837081
2216	86-87	-0.7922875872461077
2216	88-89	-0.849587138968193
2216	90-91	-0.7995623132961249
2216	92-93	-0.9124844719558354
2216	94-95	-0.8796263033484948
2216	96-97	-0.9949670077349104
2216	98-99	-1.0474947630733134
2216	100	-1.0384830858322758
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	99405.0
3	22529.0
4	16393.0
5	14497.0
6	9955.0
7	8808.0
8	9917.0
9	10032.0
10	9926.0
11	10672.0
12	12204.0
13	13520.0
14	14186.0
15	14351.0
16	14272.0
17	14412.0
18	14265.0
19	13920.0
20	13747.0
21	14563.0
22	17970.0
23	29087.0
24	49408.0
25	65491.0
26	65329.0
27	48198.0
28	45732.0
29	55162.0
30	67234.0
31	79325.0
32	102620.0
33	137992.0
34	195661.0
35	304659.0
36	533454.0
37	1088196.0
38	1922030.0
39	1064606.0
40	4473.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.342765091171124	22.060496665316887	14.197795888299916	22.39894235521207
2	14.141099994813905	23.024270442486287	41.0214808693308	21.81314869336901
3	16.279569743998227	30.009108572157057	31.301600992518065	22.40972069132666
4	11.252134647014962	19.99551716098851	41.04446937764965	27.70787881434688
5	11.425662539042184	40.8231978457978	35.87048198042484	11.880657634735178
6	26.211244627461443	42.03208920200231	19.539799694968096	12.216866475568146
7	23.404254294297825	35.87056679769969	24.86848128376075	15.856697624241736
8	22.88487478165846	38.043585940787715	22.22670719843499	16.844832079118834
9	24.493108041953047	18.775068434689246	22.11847369730039	34.613349826057316
10-11	21.479629510993625	29.542664085503983	30.50644961522597	18.47125678827642
12-13	22.517079972210276	27.817992707685573	29.786827689087104	19.878099631017047
14-15	20.49784520441778	29.06454849482218	27.21487312307358	23.22273317768646
16-17	19.852522421803663	32.035213699750535	27.64664306755675	20.46562081088905
18-19	19.96026621491503	31.175093738946448	29.288369145440228	19.576270900698294
20-21	21.55994965480401	30.236419794415752	28.524729693213175	19.678900857567054
22-23	21.04942502658472	30.234324486316353	28.62157146180735	20.094679025291573
24-25	20.750534544405358	30.35420661600356	28.531706025544135	20.363552814046944
26-27	20.60181583735014	30.279273260448726	28.55204095050882	20.56686995169231
28-29	20.61539921399454	30.179188179700688	28.758464924301574	20.4469476820032
30-31	20.8877571549152	30.466413977326678	28.361102989450725	20.2847258783074
32-33	20.45718338248878	30.284001752673046	28.796228638093087	20.462586226745092
34-35	20.724684383179024	30.043892289282248	28.73085663099184	20.500566696546883
36-37	20.963726122519166	30.216068813450303	28.379912594343054	20.440292469687474
38-39	20.757390456730604	30.23532798636396	28.54247157405485	20.464809982850586
40-41	20.684536353274403	30.016019071959942	28.69384754923613	20.605597025529523
42-43	20.577458884194648	30.07411771071614	28.708233725918607	20.640189679170597
44-45	20.752605768503617	30.072512110639977	28.594533156524655	20.580348964331755
46-47	20.746030836191704	30.157335962664018	28.537686885827867	20.558946315316412
48-49	20.73501641966918	30.007308691546726	28.599502488760397	20.6581724000237
50-51	20.714721634706397	29.948672176765008	28.513699220689894	20.822906967838705
52-53	20.67499908882196	29.988707814664302	28.557748858779608	20.77854423773414
54-55	20.785994222087567	30.189279376179414	28.40419729549512	20.6205291062379
56-57	20.677648328947637	29.98727080259613	28.44311704134147	20.891963827114765
58-59	20.68088361310112	29.722844526051745	28.608261037175904	20.98801082367123
60-61	20.921410532511718	29.981771622335245	28.369139017831955	20.727678827321082
62-63	20.704648156397663	29.982793585402394	28.460467834970952	20.85209042322899
64-65	20.734774210008382	29.814309080361756	28.546310097028048	20.904606612601814
66-67	20.613295877894757	29.786546709073775	28.516300292813284	21.083857120218184
68-69	20.8712836981337	29.953055464972948	28.363736173575642	20.811924663317708
70-71	20.842463176766454	29.81347262235114	28.437136181057742	20.906928019824665
72-73	20.701186426064282	29.820264310673338	28.62657290604462	20.851976357217758
74-75	20.745661548174184	29.81343248234924	28.498301515959422	20.942604453517156
76-77	20.788346426199155	29.844942383844064	28.39615323911351	20.97055795084327
78-79	20.814678267448336	29.923544535572955	28.49544354782384	20.76633364915487
80-81	20.763572017023858	29.809763686175188	28.543916614123404	20.88274768267755
82-83	20.683995979894334	29.830322415268178	28.66926362046015	20.816417984377335
84-85	20.673640314824898	29.693647104951776	28.550187491154087	21.082525089069236
86-87	20.769988991876854	29.870966043087105	28.411161427639254	20.94788353739679
88-89	20.695162003413134	29.7388576415725	28.499110027510433	21.066870327503935
90-91	20.629039142901895	29.719605940136688	28.503332148637167	21.14802276832425
92-93	20.67645354018179	29.743379570236044	28.49257474626971	21.087592143312456
94-95	20.608335934459333	29.544311626455183	28.628073021634947	21.219279417450537
96-97	20.575891767607352	29.711813271505825	28.549994540853678	21.16230042003314
98-99	20.43679475328638	29.68798746188816	28.554261999460184	21.32095578536528
100	20.456539373297616	29.72976357845533	28.5163939532706	21.297303094976453
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	22806.0
1	16739.0
2	8728.0
3	5652.5
4	3859.5
5	2879.5
6	2512.5
7	2475.0
8	2528.5
9	2503.5
10	2216.0
11	2013.0
12	2011.0
13	2031.5
14	2206.0
15	2427.0
16	2814.5
17	3634.5
18	4888.5
19	6479.0
20	8921.5
21	12430.5
22	17256.0
23	24897.0
24	35065.0
25	47421.0
26	63153.5
27	82168.0
28	104184.5
29	126545.5
30	145135.5
31	162853.0
32	179783.0
33	193243.5
34	205697.0
35	217317.5
36	226155.5
37	232539.0
38	237592.5
39	238755.5
40	237955.0
41	234381.0
42	226649.0
43	220361.0
44	213061.0
45	202998.0
46	193767.5
47	184727.5
48	175053.5
49	167921.0
50	162054.0
51	152646.5
52	141509.5
53	132319.0
54	124928.0
55	117687.5
56	106552.0
57	93886.0
58	84648.5
59	76311.5
60	65986.5
61	54958.5
62	45673.0
63	37343.5
64	29430.5
65	22807.0
66	17697.0
67	13568.5
68	10252.0
69	7648.5
70	5599.0
71	4177.0
72	3171.5
73	2309.5
74	1702.5
75	1266.0
76	899.5
77	651.5
78	468.0
79	344.0
80	260.5
81	201.5
82	162.0
83	135.0
84	107.5
85	101.0
86	98.0
87	74.0
88	62.5
89	57.5
90	46.0
91	40.5
92	37.5
93	33.5
94	34.5
95	28.5
96	25.5
97	19.0
98	21.0
99	33.0
100	76.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	3.6928801751902356E-4
2	1.284480060935734E-4
3	3.211200152339335E-5
4	8.028000380848338E-5
5	3.211200152339335E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	8.028000380848338E-6
64-65	3.211200152339335E-5
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	1.0436400495102839E-4
84-85	6.502680308487154E-4
86-87	0.0011720880556038574
88-89	0.003203172151958487
90-91	0.0026091001237757098
92-93	0.0021033360997822644
94-95	0.002071224098258871
96-97	0.0019427760921652978
98-99	0.0024084001142545015
100	0.0027776881317735248
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6228201.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.044598013552964
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.49640355626984	46.22099412104179
2	15.395831255520392	19.104563226665142
3	5.024035224090354	9.351427376538494
4	2.0359649019913366	5.05282495655021
5	0.9837857505352549	3.051929571171069
6	0.5794750473988782	2.157197782484877
7	0.3405271863418698	1.4789510672487285
8	0.22686771364821612	1.1260732876445931
9	0.15766689397743694	0.8804141151187994
>10	0.706115193647264	7.417935254740781
>50	0.03615614832792911	1.5432812033497016
>100	0.01657464063529166	1.8414687689842584
>500	4.668196900227107E-4	0.20237408619944705
>1k	1.0373434069597934E-4	0.10584257668077818
>5k	0.0	0.0
>10k+	2.593358517204838E-5	0.464722605581375
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	28882	0.46372941399932344	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	1.6056000761696675E-5	0.0	1.6056000761696675E-5	0.0
4	0.0	1.6056000761696675E-5	0.0	3.211200152339335E-5	0.0
5	0.0	1.6056000761696675E-5	0.0	3.211200152339335E-5	0.0
6	0.0	1.6056000761696675E-5	0.0	3.211200152339335E-5	0.0
7	0.0	1.6056000761696675E-5	0.0	4.816800228509003E-5	0.0
8	0.0	1.6056000761696675E-5	0.0	6.42240030467867E-5	0.0
9	0.0	1.6056000761696675E-5	0.0	8.028000380848338E-5	0.0
10-11	8.028000380848338E-6	1.6056000761696675E-5	0.0	8.028000380848338E-5	0.0
12-13	1.6056000761696675E-5	1.6056000761696675E-5	0.0	8.028000380848338E-5	0.0
14-15	1.6056000761696675E-5	1.6056000761696675E-5	0.0	1.284480060935734E-4	0.0
16-17	2.408400114254501E-5	1.6056000761696675E-5	0.0	1.284480060935734E-4	0.0
18-19	3.211200152339335E-5	1.6056000761696675E-5	0.0	1.3647600647442176E-4	0.0
20-21	3.211200152339335E-5	1.6056000761696675E-5	0.0	2.0070000952120845E-4	0.0
22-23	4.816800228509003E-5	1.6056000761696675E-5	0.0	4.014000190424169E-4	0.0
24-25	4.816800228509003E-5	1.6056000761696675E-5	0.0	7.225200342763504E-4	0.0
26-27	4.816800228509003E-5	4.816800228509003E-5	0.0	0.001356732064363369	0.0
28-29	4.816800228509003E-5	4.816800228509003E-5	0.0	0.0030747241458649135	0.0
30-31	4.816800228509003E-5	4.816800228509003E-5	0.0	0.007201116341620959	0.0
32-33	4.816800228509003E-5	4.816800228509003E-5	0.0	0.015100668716375724	0.0
34-35	6.42240030467867E-5	4.816800228509003E-5	0.0	0.026074945236995405	0.0
36-37	8.028000380848338E-5	4.816800228509003E-5	0.0	0.04215502999983463	0.0
38-39	8.028000380848338E-5	4.816800228509003E-5	0.0	0.06631931114618812	0.0
40-41	1.445040068552701E-4	4.816800228509003E-5	0.0	0.09200088436452195	0.0
42-43	1.445040068552701E-4	4.816800228509003E-5	0.0	0.1188946856403639	0.0
44-45	1.445040068552701E-4	4.816800228509003E-5	0.0	0.15063739914623822	0.0
46-47	1.445040068552701E-4	4.816800228509003E-5	0.0	0.18822449692937013	0.0
48-49	1.445040068552701E-4	4.816800228509003E-5	0.0	0.2300182669120666	0.0
50-51	1.445040068552701E-4	8.028000380848338E-5	0.0	0.27584209308594887	0.0
52-53	1.445040068552701E-4	8.830800418933171E-5	0.0	0.3212083232381229	0.0
54-55	1.445040068552701E-4	9.633600457018006E-5	0.0	0.3696171655346383	0.0
56-57	1.445040068552701E-4	9.633600457018006E-5	0.0	0.4272020122664635	0.0
58-59	1.445040068552701E-4	9.633600457018006E-5	0.0	0.4921324793467648	0.0
60-61	1.5253200723611842E-4	9.633600457018006E-5	0.0	0.5655806548311463	0.0
62-63	1.6056000761696676E-4	1.1239200533187674E-4	0.0	0.6448250465905002	0.0
64-65	1.6056000761696676E-4	1.1239200533187674E-4	0.0	0.7266624824728682	0.0
66-67	1.6056000761696676E-4	1.2042000571272507E-4	0.0	0.819835454892994	0.0
68-69	1.6056000761696676E-4	1.284480060935734E-4	0.0	0.9271617919845554	0.0
70-71	1.6056000761696676E-4	1.3647600647442176E-4	0.0	1.0496690777963011	0.0
72-73	1.6056000761696676E-4	1.445040068552701E-4	0.0	1.1814567320483074	0.0
74-75	1.6056000761696676E-4	1.445040068552701E-4	0.0	1.3210315466697367	1.6056000761696675E-5
76-77	1.6056000761696676E-4	1.445040068552701E-4	0.0	1.4675184696190762	1.6056000761696675E-5
78-79	1.9267200914036011E-4	1.445040068552701E-4	0.0	1.6308240533662932	1.6056000761696675E-5
80-81	2.0872800990205678E-4	1.445040068552701E-4	0.0	1.8187755982827145	1.6056000761696675E-5
82-83	2.0872800990205678E-4	1.445040068552701E-4	0.0	2.0238027000092	1.6056000761696675E-5
84-85	2.0872800990205678E-4	1.445040068552701E-4	0.0	2.237724826157666	1.6056000761696675E-5
86-87	2.0872800990205678E-4	1.445040068552701E-4	0.0	2.465800316977567	1.6056000761696675E-5
88	2.0872800990205678E-4	1.445040068552701E-4	0.0	2.6493204056837603	1.6056000761696675E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6725	0.0	44.93792	1
GTATCAA	12260	0.0	40.099182	1
TCAACGC	13515	0.0	34.810665	4
ATCAACG	13675	0.0	34.403374	3
CAACGCA	14390	0.0	32.726624	5
AACGCAG	15130	0.0	31.157047	6
TATCAAC	15720	0.0	30.824812	2
ACGCAGA	17430	0.0	26.802992	7
CGCAGAG	17455	0.0	26.38764	8
GTACATG	25975	0.0	25.856573	1
TACATGG	26160	0.0	24.63168	2
ACATGGG	26220	0.0	23.589432	3
GCAGAGT	20015	0.0	22.14371	9
CATGGGT	10920	0.0	21.692131	4
CATGGGG	12080	0.0	19.725832	4
AGAGTAC	17655	0.0	18.288721	10-11
CAGAGTA	19165	0.0	17.902279	10-11
ATGGGTG	7190	0.0	16.472744	5
GAGTACA	18980	0.0	15.872893	1
CTATGCG	285	1.7505665E-5	14.841983	9
>>END_MODULE