FastQCFastQC Report
Wed 25 May 2016
SRR2431366_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431366_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6228201
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT388960.6245142056269539No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT258170.4145177716647231No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT215980.3467775044511248No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64320.10327219689923303No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA118600.062.3398631
GTATCAA260000.049.551581
ATCAACG346800.036.8886263
TCAACGC349450.036.649244
TATCAAC352400.036.4357952
CAACGCA360900.035.4723325
AACGCAG374900.034.2103586
ACGCAGA421250.030.3457957
CGCAGAG422850.030.0975978
GCAGAGT459850.027.3590939
TATAACG5700.026.3850882
GTACATG238100.025.860311
TACATGG238650.025.2273222
ACATGGG240050.024.1991563
AGAGTAC434050.023.71772410-11
GTGGTAT42200.023.1671561
CATGGGT102550.022.7315734
ATAACGC6000.022.7159143
AGTACTT335450.022.30410412-13
GAGTACT319600.021.96180312-13
Produced by FastQC (version 0.11.5)