FastQCFastQC Report
Wed 25 May 2016
SRR2431365_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431365_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5010061
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA265840.5306123019260643No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63250.043.6199151
GTATCAA114950.036.7176251
TCAACGC127450.031.9722714
ATCAACG129250.031.5270083
TATCAAC136250.030.5281872
CAACGCA134900.030.0672045
AACGCAG141150.028.8690476
ACGCAGA163200.024.5653557
GTACATG238600.024.3081531
CGCAGAG164000.024.3022358
TACATGG240000.023.5585522
ACATGGG246650.022.1611773
GCAGAGT188500.020.1711859
CATGGGG153700.019.5704274
AGAGTAC161750.017.85549410-11
CAGAGTA178750.017.18279310-11
GTACCTG39650.016.8326451
TACCTGG37150.016.8262392
GAGTACA177550.015.9096631
TATAACG3553.4004188E-815.8871912
Produced by FastQC (version 0.11.5)