##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431365_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5010061
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.163726948633958	33.0	31.0	34.0	28.0	34.0
2	31.265731095888853	34.0	31.0	34.0	28.0	34.0
3	31.36826198323733	34.0	31.0	34.0	28.0	34.0
4	34.57758159032395	37.0	35.0	37.0	32.0	37.0
5	34.70652213615762	37.0	35.0	37.0	32.0	37.0
6	34.75159464126285	37.0	35.0	37.0	32.0	37.0
7	34.67288801473675	37.0	35.0	37.0	32.0	37.0
8	34.65040485535007	37.0	35.0	37.0	32.0	37.0
9	36.159873103341454	39.0	37.0	39.0	32.0	39.0
10-11	36.171373462319124	39.0	37.0	39.0	32.0	39.0
12-13	36.10915276281067	39.0	37.0	39.0	32.0	39.0
14-15	37.49864692266222	41.0	38.0	41.0	31.5	41.0
16-17	37.510370532414676	41.0	38.0	41.0	32.0	41.0
18-19	37.539426066868245	41.0	38.5	41.0	31.0	41.0
20-21	37.58952805165446	41.0	39.0	41.0	31.0	41.0
22-23	37.594785672270255	41.0	38.0	41.0	31.0	41.0
24-25	37.61580407903217	41.0	38.0	41.0	31.0	41.0
26-27	37.61409342121783	41.0	38.0	41.0	31.0	41.0
28-29	37.58449857197348	41.0	38.0	41.0	31.0	41.0
30-31	37.52346697974336	40.0	38.0	41.0	31.0	41.0
32-33	37.454269019878204	40.0	38.0	41.0	30.0	41.0
34-35	37.38624639500397	40.0	38.0	41.0	30.0	41.0
36-37	37.29993956161412	40.0	38.0	41.0	30.0	41.0
38-39	37.21232555851117	40.0	38.0	41.0	30.0	41.0
40-41	37.131585823006944	40.0	38.0	41.0	30.0	41.0
42-43	37.022808005730866	40.0	38.0	41.0	30.0	41.0
44-45	36.91060837383018	40.0	37.0	41.0	30.0	41.0
46-47	36.8003722908763	40.0	37.0	41.0	30.0	41.0
48-49	36.687428356660725	40.0	37.0	41.0	30.0	41.0
50-51	36.56513543447875	40.0	36.5	41.0	30.0	41.0
52-53	36.41548486136197	40.0	36.0	41.0	29.0	41.0
54-55	36.26973244437543	40.0	36.0	41.0	29.0	41.0
56-57	36.09106156591706	40.0	35.0	41.0	29.0	41.0
58-59	35.88956861004287	39.0	35.0	41.0	29.0	41.0
60-61	35.73321821830113	39.0	35.0	41.0	28.5	41.0
62-63	35.58891728064788	39.0	35.0	41.0	28.0	41.0
64-65	35.350213001398586	39.0	35.0	41.0	28.0	41.0
66-67	35.06753211188446	38.0	35.0	40.0	28.0	41.0
68-69	34.771102986570426	37.5	34.0	40.0	28.0	41.0
70-71	34.527721418960766	37.0	34.0	40.0	28.0	41.0
72-73	34.282366921280996	36.5	34.0	39.5	27.5	41.0
74-75	33.93026831409837	36.0	34.0	39.0	27.0	41.0
76-77	33.572305506859095	36.0	34.0	39.0	26.5	40.5
78-79	33.21840941657197	35.0	34.0	37.5	26.0	39.5
80-81	32.857966100612344	35.0	33.5	37.0	26.0	39.0
82-83	32.51041514264996	35.0	33.0	37.0	26.0	39.0
84-85	32.19930575695585	35.0	33.0	36.0	25.0	38.0
86-87	31.915138158996466	35.0	33.0	36.0	25.0	37.0
88-89	31.64931894042807	35.0	33.0	36.0	24.5	37.0
90-91	31.427873433077963	35.0	33.0	35.0	24.0	36.5
92-93	31.21252425868667	35.0	33.0	35.0	23.0	36.0
94-95	31.000565162779456	35.0	32.0	35.0	21.5	36.0
96-97	30.812455277490635	35.0	32.0	35.0	20.0	36.0
98-99	30.688679339433193	35.0	32.0	35.0	19.0	36.0
100	30.64440333161612	35.0	32.0	35.0	18.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.42832261962421825
1101	2	0.48754682569639485
1101	3	0.4653265157294122
1101	4	0.4611175293280425
1101	5	0.41308545267359165
1101	6	0.3984812837727034
1101	7	0.4505235546755628
1101	8	0.4121591382352605
1101	9	0.4936511699930435
1101	10-11	0.41908056128909976
1101	12-13	0.42249868198728535
1101	14-15	0.49644094040030495
1101	16-17	0.5056111670928658
1101	18-19	0.4910403213305301
1101	20-21	0.4666154439573802
1101	22-23	0.5254350651164046
1101	24-25	0.5289195439785459
1101	26-27	0.5458317254097977
1101	28-29	0.5203334281269463
1101	30-31	0.6033531561798782
1101	32-33	0.579249376577387
1101	34-35	0.6244560093082967
1101	36-37	0.5890937865939989
1101	38-39	0.5924205177427737
1101	40-41	0.6238489343471016
1101	42-43	0.594822496651318
1101	44-45	0.5702673321751703
1101	46-47	0.5558173134465108
1101	48-49	0.5404918793273978
1101	50-51	0.5434314860643852
1101	52-53	0.5327340253107167
1101	54-55	0.5790061991207622
1101	56-57	0.5839057384255355
1101	58-59	0.5785756548704484
1101	60-61	0.6174022077115282
1101	62-63	0.6274495515184455
1101	64-65	0.577151467640654
1101	66-67	0.5707590058345176
1101	68-69	0.6055186340724674
1101	70-71	0.590468013238187
1101	72-73	0.6241416300080331
1101	74-75	0.6091584096067137
1101	76-77	0.6217826773198709
1101	78-79	0.5666575527291897
1101	80-81	0.559202729809904
1101	82-83	0.5667191547193298
1101	84-85	0.6075847165680486
1101	86-87	0.6054048685898046
1101	88-89	0.6293360107824704
1101	90-91	0.5813442043038144
1101	92-93	0.5506160631378663
1101	94-95	0.5696514416893166
1101	96-97	0.5338839716959036
1101	98-99	0.5641652047242829
1101	100	0.5861391941469947
1102	1	0.1608442790775193
1102	2	0.171009382301893
1102	3	0.21464941381781344
1102	4	0.14050659263361354
1102	5	0.16352155585695272
1102	6	0.0904833106211882
1102	7	0.20847211693985912
1102	8	0.16955241975632873
1102	9	0.2416744578477008
1102	10-11	0.25291424364023385
1102	12-13	0.22287550071308004
1102	14-15	0.29532076970434673
1102	16-17	0.2835562914365255
1102	18-19	0.22894175481802392
1102	20-21	0.28616426482807356
1102	22-23	0.1699556085980447
1102	24-25	0.23636839046149305
1102	26-27	0.323405822374653
1102	28-29	0.3270022502567329
1102	30-31	0.36029929898908364
1102	32-33	0.34804011504544263
1102	34-35	0.36445019705835335
1102	36-37	0.3544722720227398
1102	38-39	0.2722489426328778
1102	40-41	0.3351536375233479
1102	42-43	0.37229659758772726
1102	44-45	0.3128788152973314
1102	46-47	0.3758120434437089
1102	48-49	0.25705930001268484
1102	50-51	0.2561302008894799
1102	52-53	0.2498045923204728
1102	54-55	0.27663708325533776
1102	56-57	0.2792082182655662
1102	58-59	0.2567589867836091
1102	60-61	0.25802232203099607
1102	62-63	0.2175886090142285
1102	64-65	0.22535772394304843
1102	66-67	0.27031265405728533
1102	68-69	0.22948278526573063
1102	70-71	0.23577032708418955
1102	72-73	0.2540395449140931
1102	74-75	0.2627850162597767
1102	76-77	0.2593315640963354
1102	78-79	0.23311798719333154
1102	80-81	0.2343247323421167
1102	82-83	0.2507688075889476
1102	84-85	0.2907729834220092
1102	86-87	0.28469332107426837
1102	88-89	0.2589206888210853
1102	90-91	0.2865720200327395
1102	92-93	0.3185210083211061
1102	94-95	0.30471865020965083
1102	96-97	0.24708718538935415
1102	98-99	0.24495550349262274
1102	100	0.2523513445897123
1103	1	0.11974773190687671
1103	2	0.20081333899183562
1103	3	0.22271466332132306
1103	4	0.1686945871214931
1103	5	0.13137060065049866
1103	6	0.1635491794957531
1103	7	0.2280418897178933
1103	8	0.28974447589057206
1103	9	0.312250876919272
1103	10-11	0.3043630283871721
1103	12-13	0.26488767867562757
1103	14-15	0.30251243020367724
1103	16-17	0.31902522184557824
1103	18-19	0.24021783684951714
1103	20-21	0.26488686130940664
1103	22-23	0.267539140742592
1103	24-25	0.2654942433338121
1103	26-27	0.33082044834146984
1103	28-29	0.2860531434125093
1103	30-31	0.28572211606710596
1103	32-33	0.24983112765100657
1103	34-35	0.2644238606060938
1103	36-37	0.3140907800142472
1103	38-39	0.2550188673373128
1103	40-41	0.2264629206015556
1103	42-43	0.22259386324501662
1103	44-45	0.1999863735630356
1103	46-47	0.1998213681621479
1103	48-49	0.24605749349349537
1103	50-51	0.15877448128780713
1103	52-53	0.13150612020787378
1103	54-55	0.15271217065885168
1103	56-57	0.08115635317991376
1103	58-59	0.09566014952195445
1103	60-61	0.1047805736799603
1103	62-63	0.0913295558334184
1103	64-65	0.15019173290595944
1103	66-67	0.14878241978576057
1103	68-69	0.11776455261243512
1103	70-71	0.07929779004543747
1103	72-73	0.09362903291771829
1103	74-75	0.0816099578251368
1103	76-77	0.13779725341413496
1103	78-79	0.08561560198607765
1103	80-81	0.11144301582682203
1103	82-83	0.04575078255273013
1103	84-85	0.10441520484760503
1103	86-87	0.14296769652961316
1103	88-89	0.14469489574423378
1103	90-91	0.09006408027990176
1103	92-93	0.1479327737427809
1103	94-95	0.11372106449936581
1103	96-97	0.1444670957505032
1103	98-99	0.1256479879136343
1103	100	0.12120015242414439
1104	1	0.17721583480702563
1104	2	0.17266723784291926
1104	3	0.19195873423936405
1104	4	0.09836634402446265
1104	5	0.13869191252222635
1104	6	0.13599596197438757
1104	7	0.12421504357772761
1104	8	0.191094005996149
1104	9	0.19436929837275585
1104	10-11	0.18046414781986186
1104	12-13	0.18925783932321139
1104	14-15	0.2559887251772821
1104	16-17	0.21505007126611275
1104	18-19	0.22740049473316049
1104	20-21	0.1722150916308891
1104	22-23	0.16613678866622195
1104	24-25	0.24224468482721306
1104	26-27	0.2426521079144237
1104	28-29	0.2782407100541775
1104	30-31	0.28643607256539383
1104	32-33	0.2511593029393637
1104	34-35	0.28504722865288556
1104	36-37	0.24352859700749008
1104	38-39	0.2371813840806496
1104	40-41	0.2449193594278185
1104	42-43	0.2261181058270978
1104	44-45	0.1872474929564092
1104	46-47	0.2542713071834868
1104	48-49	0.24847845954100478
1104	50-51	0.20807445013512194
1104	52-53	0.22238106824579518
1104	54-55	0.20121331407025878
1104	56-57	0.2670389616320179
1104	58-59	0.253881351229154
1104	60-61	0.2801671961104688
1104	62-63	0.2348726079507628
1104	64-65	0.27991960393976
1104	66-67	0.3023803084793002
1104	68-69	0.3408996913597804
1104	70-71	0.27737014680945293
1104	72-73	0.2548329217940477
1104	74-75	0.2558000618868803
1104	76-77	0.2746002515400576
1104	78-79	0.2381258396210626
1104	80-81	0.25893971629627544
1104	82-83	0.22587491172726004
1104	84-85	0.22903450830515482
1104	86-87	0.20357705078471255
1104	88-89	0.21537812213969332
1104	90-91	0.19209317118184543
1104	92-93	0.1556232104600923
1104	94-95	0.21098641947774865
1104	96-97	0.2585366674453944
1104	98-99	0.24149887370123224
1104	100	0.3427514378897989
1105	1	0.17279758127849476
1105	2	0.2156221983947617
1105	3	0.23851083722023958
1105	4	0.2430260404959057
1105	5	0.2528819297411218
1105	6	0.2134799216147556
1105	7	0.2267632716836161
1105	8	0.26606365762079065
1105	9	0.22105527967837446
1105	10-11	0.2760335090279966
1105	12-13	0.33862022227176425
1105	14-15	0.38651510384674737
1105	16-17	0.35688788903050295
1105	18-19	0.373709721107204
1105	20-21	0.3604255554858824
1105	22-23	0.3848784797624205
1105	24-25	0.42713116901036585
1105	26-27	0.3955270869446039
1105	28-29	0.42866224209333126
1105	30-31	0.4070724202269034
1105	32-33	0.41625861652298823
1105	34-35	0.438666707366707
1105	36-37	0.40466537302587824
1105	38-39	0.46070040178192073
1105	40-41	0.4100885442156468
1105	42-43	0.448156327904627
1105	44-45	0.43987283525611787
1105	46-47	0.41393297245382144
1105	48-49	0.4057508940168475
1105	50-51	0.4424793875143749
1105	52-53	0.49360206704160703
1105	54-55	0.46731698887943907
1105	56-57	0.43378936821014946
1105	58-59	0.41837191518176553
1105	60-61	0.47235136816209433
1105	62-63	0.5161389214781309
1105	64-65	0.48443830354685247
1105	66-67	0.4302540703885427
1105	68-69	0.3959218210575841
1105	70-71	0.41632118270630514
1105	72-73	0.42555318359701744
1105	74-75	0.4046533547590272
1105	76-77	0.3520738868089879
1105	78-79	0.3261056926605548
1105	80-81	0.30072160125570235
1105	82-83	0.3818563617090618
1105	84-85	0.419716320038205
1105	86-87	0.30532029651022796
1105	88-89	0.3106426704683507
1105	90-91	0.35278283322046633
1105	92-93	0.3175531610434916
1105	94-95	0.342187197638669
1105	96-97	0.31914582527238267
1105	98-99	0.3590428281943474
1105	100	0.3478228647189283
1106	1	0.2615847097430546
1106	2	0.2710410166493382
1106	3	0.3163279147124882
1106	4	0.28847244178106024
1106	5	0.3167824584406276
1106	6	0.29651293074086027
1106	7	0.25932079760509197
1106	8	0.32144285297552955
1106	9	0.37060706734087745
1106	10-11	0.3538746292699386
1106	12-13	0.4542382467569226
1106	14-15	0.4281393244119087
1106	16-17	0.3740746440870595
1106	18-19	0.40156840264427984
1106	20-21	0.4371544043251916
1106	22-23	0.4530019016767568
1106	24-25	0.4663640887243332
1106	26-27	0.5046093785515495
1106	28-29	0.4857570753848819
1106	30-31	0.5133813890636247
1106	32-33	0.44923380816023695
1106	34-35	0.507584408698257
1106	36-37	0.4865033061743702
1106	38-39	0.4876962904717175
1106	40-41	0.45244321041737834
1106	42-43	0.45248763766788613
1106	44-45	0.4622035338067221
1106	46-47	0.4507678660719421
1106	48-49	0.45562497221698095
1106	50-51	0.3984763078006708
1106	52-53	0.3912702117489246
1106	54-55	0.4439764387981384
1106	56-57	0.45009127722128284
1106	58-59	0.46107125017276473
1106	60-61	0.43378116526650956
1106	62-63	0.42506731146520593
1106	64-65	0.42672956750446644
1106	66-67	0.418258307516858
1106	68-69	0.4438603458202408
1106	70-71	0.42576401467993463
1106	72-73	0.3453411063690055
1106	74-75	0.3691153685315456
1106	76-77	0.4414592538086666
1106	78-79	0.46594636713085436
1106	80-81	0.4208303798464641
1106	82-83	0.42832654138575066
1106	84-85	0.4223721191578349
1106	86-87	0.3984325699304243
1106	88-89	0.40553234198890564
1106	90-91	0.4053311769412211
1106	92-93	0.45595166283831645
1106	94-95	0.45612433128447094
1106	96-97	0.4427344416076515
1106	98-99	0.4114053029655764
1106	100	0.49037199137024956
1107	1	0.06313322579802971
1107	2	0.06744311621871191
1107	3	0.11390789258767953
1107	4	0.07541238195773303
1107	5	0.031238666253138092
1107	6	0.09656187363543722
1107	7	0.08207980453512675
1107	8	0.05397177053653479
1107	9	0.07854555275362429
1107	10-11	0.05761537997940991
1107	12-13	0.06332102525887251
1107	14-15	0.0783739017154943
1107	16-17	0.08145817290315449
1107	18-19	0.12442641559414369
1107	20-21	0.10003566798812358
1107	22-23	0.15439308959840758
1107	24-25	0.1409740172269096
1107	26-27	0.1701848971407145
1107	28-29	0.17563248403117626
1107	30-31	0.17024440962822496
1107	32-33	0.22816338952552684
1107	34-35	0.19722495288795017
1107	36-37	0.27637900197368026
1107	38-39	0.2640227546040208
1107	40-41	0.24656266887750178
1107	42-43	0.22082975370982183
1107	44-45	0.24328787301870847
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2216	60-61	-0.8319191797906313
2216	62-63	-0.9123849154953305
2216	64-65	-0.9355582879882292
2216	66-67	-1.0073286224978588
2216	68-69	-1.010709729768827
2216	70-71	-1.0359517443014923
2216	72-73	-0.9502345189758046
2216	74-75	-1.0008570700194852
2216	76-77	-0.9314112911555981
2216	78-79	-0.8852407136434124
2216	80-81	-0.8751621942003993
2216	82-83	-0.914846629155285
2216	84-85	-0.8953190418311294
2216	86-87	-0.8608381221070935
2216	88-89	-0.9749912701321826
2216	90-91	-0.9250281778324556
2216	92-93	-0.998202181787299
2216	94-95	-1.0224983989856682
2216	96-97	-1.1631437785947192
2216	98-99	-1.2417354220589232
2216	100	-1.1698859012924032
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	88912.0
3	19538.0
4	13934.0
5	12516.0
6	8858.0
7	7318.0
8	7772.0
9	8343.0
10	8431.0
11	8967.0
12	10131.0
13	11067.0
14	11652.0
15	12078.0
16	12135.0
17	12319.0
18	12214.0
19	12039.0
20	11853.0
21	12833.0
22	15146.0
23	23823.0
24	38906.0
25	51650.0
26	52823.0
27	40025.0
28	39956.0
29	48134.0
30	57222.0
31	68177.0
32	88050.0
33	117175.0
34	167170.0
35	261522.0
36	450385.0
37	891854.0
38	1493859.0
39	797842.0
40	3432.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.68356773270627	21.906356417584774	14.226036962985717	22.184038886723236
2	14.345174722667661	22.88685910371425	40.458306437415644	22.309659736202445
3	16.84866049508389	29.65353708338822	30.74823455208121	22.749567869446675
4	11.756737201487807	19.770621280604704	39.96736562772265	28.50527589018484
5	12.00905777575518	40.57875554384579	35.03295369716131	12.379232983237726
6	26.86498228265085	41.216224712633235	19.21377803583629	12.705014968879622
7	23.97717313222334	35.6747352976341	24.033779229434533	16.314312340708025
8	23.279357277286643	37.80409060887682	21.794485137007314	17.122066976829224
9	24.593991969359255	18.975517463759424	21.81129131960669	34.619199247274636
10-11	21.983993807660227	29.607324142360742	29.639738917350506	18.768943132628525
12-13	22.93162698019046	27.677796737404993	29.20800764701268	20.182568635391863
14-15	20.851522566292108	29.02030534159165	26.6391866286658	23.488985463450447
16-17	20.247198587003233	32.03134652452335	27.101316730474938	20.620138157998475
18-19	20.29606026752968	31.111198047289246	28.75481955209727	19.83792213308381
20-21	21.972467001898778	30.043566335819065	28.078310822962038	19.90565583932012
22-23	21.564527857046052	30.059733803640317	28.006006314094776	20.369732025218852
24-25	21.181099391803812	30.115122350805706	28.0627720900005	20.64100616738998
26-27	20.978297469831205	30.16289622022566	28.029618801048528	20.829187508894602
28-29	20.87330872817716	30.10645978162741	28.269406300641847	20.75082518955358
30-31	21.11139964164109	30.220280751072693	27.97630607691204	20.692013530374183
32-33	20.836482829250983	30.105641428317938	28.31730990900111	20.740565833429972
34-35	21.0832263319748	29.917390227384455	28.187171772958454	20.81221166768229
36-37	21.145491042923428	30.044753946109637	27.97825216100163	20.8315028499653
38-39	21.02058238412666	30.104393938516914	28.07428891584354	20.800734761512885
40-41	20.988157629218488	29.825105921864026	28.237580340838164	20.949156108079322
42-43	20.87599332622896	29.958188133837094	28.193688659678994	20.97212988025495
44-45	21.02972398938855	29.94271926030442	28.178249725901537	20.84930702440549
46-47	20.993955961813636	29.856492765257748	28.216971809325276	20.932579463603336
48-49	20.964325584059754	29.80259122593517	28.26579357017809	20.967289619826985
50-51	20.97486437789879	29.800824780376928	28.167710932062505	21.056599909661777
52-53	20.94916608799773	29.786583436808456	28.207690485205667	21.05655998998815
54-55	21.0378376630544	29.947280083016953	28.10716276708008	20.907719486848563
56-57	20.997818190237606	29.722981416793125	28.15852940712698	21.12067098584229
58-59	20.955762814065537	29.595547838639092	28.279925134644067	21.168764212651304
60-61	21.13007206898279	29.856572604605013	28.066364860627445	20.946990465784747
62-63	20.986383298165762	29.730000266463847	28.196615589771817	21.08700084559857
64-65	21.084994881798334	29.622596373836203	28.1932224171744	21.09918632719106
66-67	20.939066410568653	29.66334142438585	28.1723416142039	21.225250550841597
68-69	21.121229861273147	29.695905898151736	28.144777079560505	21.03808716101461
70-71	21.11281679005505	29.642523314586388	28.1403759355425	21.10428395981606
72-73	21.03565206092301	29.668640761060594	28.27858782557737	21.017119352439025
74-75	21.070871192985475	29.655876445416535	28.202401128449335	21.07085123314866
76-77	21.134074016264474	29.55762414868801	28.184197757272816	21.124104077774703
78-79	21.12974273167532	29.640287812862958	28.188918258679884	21.041051196781837
80-81	21.04455624837265	29.578295511601105	28.27665454339324	21.100493696633006
82-83	20.996613710167484	29.587915742649955	28.357809609095145	21.057660938087412
84-85	21.05051739746287	29.46620142627988	28.251058153085616	21.232223023171635
86-87	21.109836105821138	29.589574932220575	28.18403629061152	21.11655267134677
88-89	21.000062875694752	29.504978657194727	28.288693253437952	21.20626521367257
90-91	21.0239999744507	29.420907151847874	28.220249736423042	21.334843137278384
92-93	21.068160651246977	29.504788263694024	28.236254675188892	21.190796409870103
94-95	20.90750270835631	29.36530898715209	28.34711671705798	21.380071587433626
96-97	21.0004691654693	29.485108612155447	28.1983916237247	21.31603059865055
98-99	20.82101445051861	29.51535791760514	28.287964048955633	21.37566358292062
100	20.82154998826323	29.5250041118618	28.22284135239534	21.43060454747963
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	21471.0
1	14860.5
2	6611.0
3	4134.0
4	2856.5
5	2197.0
6	1937.0
7	1936.0
8	2057.0
9	2103.5
10	1812.5
11	1584.5
12	1598.0
13	1646.0
14	1799.0
15	2007.0
16	2436.5
17	3416.0
18	4587.5
19	6005.0
20	8910.5
21	12412.5
22	15654.0
23	20747.5
24	29207.0
25	37886.5
26	48135.0
27	62388.5
28	78801.0
29	96678.5
30	112319.5
31	126591.0
32	138707.0
33	147617.5
34	156993.0
35	165625.5
36	171680.5
37	175023.5
38	177780.0
39	179916.0
40	181235.5
41	179698.5
42	174647.5
43	170011.5
44	164888.0
45	159445.0
46	154474.0
47	146936.0
48	140898.0
49	137279.0
50	132623.5
51	127768.5
52	121547.0
53	115359.0
54	109819.0
55	103475.0
56	94368.0
57	83841.5
58	75824.0
59	69842.0
60	62037.5
61	52434.0
62	44299.0
63	36811.0
64	29738.0
65	23970.0
66	18897.5
67	14444.0
68	11058.0
69	8457.0
70	6163.5
71	4546.5
72	3480.0
73	2672.5
74	2024.0
75	1491.5
76	1015.0
77	680.5
78	490.0
79	341.5
80	243.5
81	199.0
82	169.5
83	134.0
84	107.5
85	92.0
86	89.0
87	75.0
88	58.5
89	58.5
90	52.0
91	45.5
92	39.0
93	32.5
94	28.0
95	23.5
96	17.5
97	18.5
98	19.0
99	26.0
100	62.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	4.391164099598787E-4
2	1.3971885771450686E-4
3	1.9959836816358123E-5
4	1.1975902089814873E-4
5	1.9959836816358123E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	9.979918408179062E-6
64-65	9.979918408179062E-6
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	9.979918408179062E-6
82-83	1.0977910248996967E-4
84-85	6.886143701643553E-4
86-87	0.0011975902089814874
88-89	0.0035129312796790295
90-91	0.002774417317473779
92-93	0.002055863192084887
94-95	0.002065843110493066
96-97	0.0020259234368603498
98-99	0.002734497643841063
100	0.0032933730746990903
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5010061.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.55824676207825
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.30068543355844	44.252185576434265
2	15.102632697057505	17.98972649856764
3	4.852787380129133	8.670705248088902
4	2.1567804685126655	5.138162534212322
5	1.0865307992010032	3.2355934726705717
6	0.6288639345225494	2.247242003523925
7	0.39354080547176884	1.640702028226425
8	0.28071070537853854	1.3374909967753636
9	0.20048910930350358	1.074670186050661
>10	0.9264154269022692	9.484129376579334
>50	0.04892507451164698	1.9807362581575745
>100	0.020967323715628747	2.1163725440203867
>500	5.031317879314499E-4	0.1933781950020258
>1k	1.3416795801770453E-4	0.10783595869801726
>5k	0.0	0.0
>10k+	3.3541989502723956E-5	0.5310691229926452
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	26584	0.5306123019260643	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.991967363271625E-5	0.0	0.0	0.0
3	0.0	3.991967363271625E-5	0.0	0.0	0.0
4	0.0	3.991967363271625E-5	0.0	1.9959836816358123E-5	0.0
5	0.0	3.991967363271625E-5	0.0	1.9959836816358123E-5	0.0
6	0.0	3.991967363271625E-5	0.0	1.9959836816358123E-5	0.0
7	0.0	3.991967363271625E-5	0.0	1.9959836816358123E-5	0.0
8	0.0	3.991967363271625E-5	0.0	1.9959836816358123E-5	0.0
9	0.0	3.991967363271625E-5	0.0	1.9959836816358123E-5	0.0
10-11	0.0	3.991967363271625E-5	0.0	4.989959204089531E-5	0.0
12-13	0.0	3.991967363271625E-5	0.0	5.9879510449074374E-5	0.0
14-15	0.0	3.991967363271625E-5	0.0	5.9879510449074374E-5	0.0
16-17	0.0	3.991967363271625E-5	0.0	5.9879510449074374E-5	0.0
18-19	0.0	3.991967363271625E-5	0.0	5.9879510449074374E-5	0.0
20-21	0.0	3.991967363271625E-5	0.0	9.979918408179062E-5	0.0
22-23	0.0	3.991967363271625E-5	0.0	3.2933730746990904E-4	0.0
24-25	0.0	3.991967363271625E-5	0.0	5.688553492662065E-4	0.0
26-27	0.0	3.991967363271625E-5	0.0	0.0010578713512669805	0.0
28-29	0.0	3.991967363271625E-5	0.0	0.0022953812338811844	0.0
30-31	0.0	3.991967363271625E-5	0.0	0.005419095695641231	0.0
32-33	0.0	3.991967363271625E-5	0.0	0.01222540005001935	0.0
34-35	0.0	3.991967363271625E-5	0.0	0.022464796336811067	0.0
36-37	0.0	3.991967363271625E-5	0.0	0.03780393093018229	0.0
38-39	0.0	3.991967363271625E-5	0.0	0.05711507305000877	0.0
40-41	0.0	3.991967363271625E-5	0.0	0.07889125501665548	0.0
42-43	0.0	3.991967363271625E-5	0.0	0.10012852138926054	0.0
44-45	0.0	3.991967363271625E-5	0.0	0.12751341750130388	0.0
46-47	0.0	3.991967363271625E-5	0.0	0.16116570237368366	0.0
48-49	0.0	3.991967363271625E-5	0.0	0.19799160129986443	0.0
50-51	0.0	3.991967363271625E-5	0.0	0.2362346486400066	0.0
52-53	0.0	3.991967363271625E-5	0.0	0.2731703266686773	0.0
54-55	0.0	3.991967363271625E-5	0.0	0.31399817287653786	0.0
56-57	0.0	3.991967363271625E-5	0.0	0.3655145116995582	0.0
58-59	0.0	3.991967363271625E-5	0.0	0.42331819911973123	0.0
60-61	0.0	3.991967363271625E-5	0.0	0.48729945603456726	0.0
62-63	0.0	3.991967363271625E-5	0.0	0.5540950499405097	0.0
64-65	0.0	3.991967363271625E-5	0.0	0.6271181129331559	0.0
66-67	0.0	3.991967363271625E-5	0.0	0.7108596082961864	0.0
68-69	0.0	3.991967363271625E-5	0.0	0.8055790139082138	0.0
70-71	0.0	3.991967363271625E-5	0.0	0.9128930765513634	0.0
72-73	0.0	5.9879510449074374E-5	0.0	1.0278317968583617	0.0
74-75	0.0	5.9879510449074374E-5	0.0	1.147800396043082	0.0
76-77	0.0	5.9879510449074374E-5	0.0	1.2803337125037	0.0
78-79	0.0	5.9879510449074374E-5	0.0	1.4300724083000187	0.0
80-81	0.0	5.9879510449074374E-5	0.0	1.6038926472152735	0.0
82-83	0.0	5.9879510449074374E-5	0.0	1.7926927436612048	0.0
84-85	0.0	5.9879510449074374E-5	0.0	1.98739097188637	0.0
86-87	0.0	7.98393472654325E-5	0.0	2.1925581345217156	0.0
88	0.0	7.98393472654325E-5	0.0	2.3613884142328807	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6325	0.0	43.619915	1
GTATCAA	11495	0.0	36.717625	1
TCAACGC	12745	0.0	31.972271	4
ATCAACG	12925	0.0	31.527008	3
TATCAAC	13625	0.0	30.528187	2
CAACGCA	13490	0.0	30.067204	5
AACGCAG	14115	0.0	28.869047	6
ACGCAGA	16320	0.0	24.565355	7
GTACATG	23860	0.0	24.308153	1
CGCAGAG	16400	0.0	24.302235	8
TACATGG	24000	0.0	23.558552	2
ACATGGG	24665	0.0	22.161177	3
GCAGAGT	18850	0.0	20.171185	9
CATGGGG	15370	0.0	19.570427	4
AGAGTAC	16175	0.0	17.855494	10-11
CAGAGTA	17875	0.0	17.182793	10-11
GTACCTG	3965	0.0	16.832645	1
TACCTGG	3715	0.0	16.826239	2
GAGTACA	17755	0.0	15.909663	1
TATAACG	355	3.4004188E-8	15.887191	2
>>END_MODULE