FastQCFastQC Report
Wed 25 May 2016
SRR2431365_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431365_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5010061
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT315840.6304114860078549No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218700.4365216311737522No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174620.34853867048724557No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50400.10059757755444496No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA106800.060.3388141
GTATCAA221650.045.6993561
ATCAACG285600.035.200823
TCAACGC287400.034.94734
TATCAAC289550.034.7530822
CAACGCA296850.033.8489235
AACGCAG305150.032.9744456
ACGCAGA347000.028.8892047
CGCAGAG349000.028.656328
GCAGAGT378900.026.047679
GTACATG222550.025.3656481
TACATGG226350.024.3358962
ACATGGG229100.023.1616273
AGAGTAC355550.022.5701410-11
GAGTACT242000.022.34320412-13
CAGAGTA372400.021.29019410-11
AGTACTT254550.021.14931112-13
CATGGGG141550.020.7522894
GTGGTAT43850.020.6879881
TGGTATC44200.020.0973932
Produced by FastQC (version 0.11.5)