##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431365_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5010061
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.74596916085453	34.0	31.0	34.0	31.0	34.0
2	32.88251280772829	34.0	31.0	34.0	31.0	34.0
3	32.95805480212716	34.0	31.0	34.0	31.0	34.0
4	36.34658041089719	37.0	37.0	37.0	35.0	37.0
5	36.2936682806856	37.0	37.0	37.0	35.0	37.0
6	36.322077515623064	37.0	37.0	37.0	35.0	37.0
7	36.191826806100764	37.0	37.0	37.0	35.0	37.0
8	36.24457666283904	37.0	37.0	37.0	35.0	37.0
9	38.0588523772465	39.0	39.0	39.0	35.0	39.0
10-11	38.05650090887117	39.0	39.0	39.0	35.0	39.0
12-13	38.03345687806994	39.0	39.0	39.0	35.0	39.0
14-15	39.51728781346175	41.0	39.0	41.0	36.0	41.0
16-17	39.53416924464592	41.0	39.5	41.0	36.5	41.0
18-19	39.61198276827368	41.0	40.0	41.0	37.0	41.0
20-21	39.61789776611502	41.0	40.0	41.0	37.0	41.0
22-23	39.54927435015262	41.0	40.0	41.0	36.5	41.0
24-25	39.521238763360365	41.0	40.0	41.0	36.0	41.0
26-27	39.43635287075347	41.0	39.5	41.0	36.0	41.0
28-29	39.35335418071756	41.0	39.0	41.0	36.0	41.0
30-31	39.199008954182396	41.0	39.0	41.0	35.5	41.0
32-33	39.01167969811146	41.0	39.0	41.0	35.0	41.0
34-35	38.91340524596407	41.0	39.0	41.0	35.0	41.0
36-37	38.81462531094931	41.0	38.5	41.0	35.0	41.0
38-39	38.70786074261371	41.0	38.0	41.0	35.0	41.0
40-41	38.62102018318739	41.0	38.0	41.0	35.0	41.0
42-43	38.50139130042528	41.0	38.0	41.0	35.0	41.0
44-45	38.317310208398666	41.0	38.0	41.0	34.5	41.0
46-47	38.03329250881376	41.0	38.0	41.0	33.5	41.0
48-49	37.78890736060898	40.0	38.0	41.0	33.0	41.0
50-51	37.654832945147774	40.0	38.0	41.0	33.0	41.0
52-53	37.552598161978466	40.0	38.0	41.0	33.0	41.0
54-55	37.42009378728123	40.0	37.0	41.0	33.0	41.0
56-57	37.27010459553287	40.0	37.0	41.0	33.0	41.0
58-59	37.097750207033414	40.0	36.5	41.0	32.5	41.0
60-61	36.91644373192263	40.0	36.0	41.0	32.0	41.0
62-63	36.70370839796162	39.5	35.5	41.0	32.0	41.0
64-65	36.4470660137671	39.0	35.0	41.0	31.5	41.0
66-67	36.17231656460869	39.0	35.0	41.0	31.0	41.0
68-69	35.909722256874716	38.5	35.0	41.0	31.0	41.0
70-71	35.66078736366683	37.5	35.0	40.0	31.0	41.0
72-73	35.39616573530741	37.0	35.0	40.0	31.0	41.0
74-75	35.0845128432568	36.5	35.0	39.0	31.0	41.0
76-77	34.74949396823712	36.0	35.0	39.0	31.0	41.0
78-79	34.41475672651491	36.0	35.0	38.5	30.0	40.0
80-81	34.08256466338433	35.0	35.0	37.0	30.0	39.0
82-83	33.74275722391404	35.0	35.0	37.0	30.0	39.0
84-85	33.455936764043386	35.0	34.5	36.5	30.0	38.5
86-87	33.20901831334987	35.0	34.0	36.0	30.0	37.0
88-89	32.968151285982344	35.0	34.0	36.0	29.0	37.0
90-91	32.756621825562604	35.0	34.0	35.5	29.0	36.5
92-93	32.59550043402665	35.0	34.0	35.0	29.0	36.0
94-95	32.45474945315037	35.0	34.0	35.0	29.0	36.0
96-97	32.3155519463735	35.0	34.0	35.0	29.0	36.0
98-99	32.19291671299012	35.0	34.0	35.0	29.0	36.0
100	32.0923246243908	35.0	34.0	35.0	29.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.1882066363922874
1101	2	0.17777188631931296
1101	3	0.19061335136273527
1101	4	0.13268202924532346
1101	5	0.1561815364276029
1101	6	0.20113300661895295
1101	7	0.17497709642502457
1101	8	0.21880648074002806
1101	9	0.21829727500853835
1101	10-11	0.21365823306281584
1101	12-13	0.213522769373526
1101	14-15	0.27405740319897376
1101	16-17	0.2749368666011023
1101	18-19	0.2887478601255822
1101	20-21	0.2759546335962284
1101	22-23	0.2785375283300624
1101	24-25	0.29582705538468446
1101	26-27	0.308411316852208
1101	28-29	0.27965059346205834
1101	30-31	0.2809313971867695
1101	32-33	0.27641728567400037
1101	34-35	0.2500696900932198
1101	36-37	0.2645637830727523
1101	38-39	0.2854132593503351
1101	40-41	0.2836608239982752
1101	42-43	0.2772210881873036
1101	44-45	0.3177799363721192
1101	46-47	0.267863368404484
1101	48-49	0.2170354254122202
1101	50-51	0.22651170356808592
1101	52-53	0.22004363874126653
1101	54-55	0.2074690509533923
1101	56-57	0.19423595215955913
1101	58-59	0.19589435479039707
1101	60-61	0.19644669128422265
1101	62-63	0.1725051121966814
1101	64-65	0.20839352015330093
1101	66-67	0.23406812323568715
1101	68-69	0.22640105653674425
1101	70-71	0.2238309783490351
1101	72-73	0.2031333989118167
1101	74-75	0.2019737827861121
1101	76-77	0.20915950101731084
1101	78-79	0.20129673771756273
1101	80-81	0.20266000055959665
1101	82-83	0.20257378412768645
1101	84-85	0.2309022450915137
1101	86-87	0.23038937217670963
1101	88-89	0.2378157062905828
1101	90-91	0.2741721625754465
1101	92-93	0.25426992414116967
1101	94-95	0.2629435027527265
1101	96-97	0.30313140701451147
1101	98-99	0.3111574818513816
1101	100	0.33453053755535933
1102	1	0.12993394313436113
1102	2	0.17388295378015073
1102	3	0.18615419861835392
1102	4	0.09597952139534272
1102	5	0.1263374712118761
1102	6	0.10995267477107973
1102	7	0.16434659333894786
1102	8	0.18015458827907338
1102	9	0.20079431201524045
1102	10-11	0.2308284702608958
1102	12-13	0.2083076368174659
1102	14-15	0.3013920691569112
1102	16-17	0.31517347939841045
1102	18-19	0.2800069530629301
1102	20-21	0.2850955247726432
1102	22-23	0.24107230868861507
1102	24-25	0.27605240272102805
1102	26-27	0.31120573399370954
1102	28-29	0.29818819104656313
1102	30-31	0.2963802456370601
1102	32-33	0.25498896367559354
1102	34-35	0.26377944376669404
1102	36-37	0.22277143741368377
1102	38-39	0.2521712657645949
1102	40-41	0.23226320351643182
1102	42-43	0.27618805205705144
1102	44-45	0.2458921367728948
1102	46-47	0.1716873235059282
1102	48-49	0.2506305251703296
1102	50-51	0.20090995372057563
1102	52-53	0.1898835086117927
1102	54-55	0.2150385935341177
1102	56-57	0.197091203036166
1102	58-59	0.1720730445270391
1102	60-61	0.17134572080939847
1102	62-63	0.1724302304489811
1102	64-65	0.208650073136198
1102	66-67	0.23195059282834052
1102	68-69	0.263579290957928
1102	70-71	0.24048561967099857
1102	72-73	0.19878272633476968
1102	74-75	0.17173791019918383
1102	76-77	0.1839638524706757
1102	78-79	0.15642489555402506
1102	80-81	0.16323918690116557
1102	82-83	0.19445950449809146
1102	84-85	0.19471640900484033
1102	86-87	0.1785309645856472
1102	88-89	0.13758965317224892
1102	90-91	0.17753815861397726
1102	92-93	0.2244045256196472
1102	94-95	0.16856418944416873
1102	96-97	0.12648066101893818
1102	98-99	0.1604633132951534
1102	100	0.19761750010206924
1103	1	0.11541028003684772
1103	2	0.13716783826278345
1103	3	0.13202859114190346
1103	4	0.10806204319846557
1103	5	0.11299617001672146
1103	6	0.1263545694380923
1103	7	0.09935834516447528
1103	8	0.12130594187861732
1103	9	0.20862126387977042
1103	10-11	0.20414173061796248
1103	12-13	0.19196082439619744
1103	14-15	0.26712069085715484
1103	16-17	0.27695197803161165
1103	18-19	0.23956942561813577
1103	20-21	0.21633327069682196
1103	22-23	0.2369915782347647
1103	24-25	0.24062263424736585
1103	26-27	0.24427386943372653
1103	28-29	0.2735590856041341
1103	30-31	0.3315395107993382
1103	32-33	0.3549159857841815
1103	34-35	0.3185470757842026
1103	36-37	0.29418264860574794
1103	38-39	0.29321273068948983
1103	40-41	0.2734765266704997
1103	42-43	0.3054046608386827
1103	44-45	0.3123486228939072
1103	46-47	0.3561819390338812
1103	48-49	0.34665812583718747
1103	50-51	0.3288490559578534
1103	52-53	0.30760171076298803
1103	54-55	0.27320942004605797
1103	56-57	0.29620831738195363
1103	58-59	0.33530392569451806
1103	60-61	0.255816616088957
1103	62-63	0.33839386837901486
1103	64-65	0.37651094560489895
1103	66-67	0.37733894334709817
1103	68-69	0.37444786577417943
1103	70-71	0.3402599082034854
1103	72-73	0.29681878062558553
1103	74-75	0.31620422124059644
1103	76-77	0.28581147242687166
1103	78-79	0.2612398382721395
1103	80-81	0.2629872525530388
1103	82-83	0.2784213757401375
1103	84-85	0.27823192341825376
1103	86-87	0.24261163338631775
1103	88-89	0.23815502902421315
1103	90-91	0.2897772997657526
1103	92-93	0.2804264955668003
1103	94-95	0.2360545831086256
1103	96-97	0.25751195398095916
1103	98-99	0.27083353169584967
1103	100	0.3225394905813559
1104	1	0.05274893716968165
1104	2	0.11018686694781366
1104	3	0.12479774979355085
1104	4	0.018349699367234962
1104	5	0.06841454459377871
1104	6	0.07333668072489274
1104	7	0.04743885862350794
1104	8	0.09142478394239362
1104	9	0.16277763067160578
1104	10-11	0.17297397688332694
1104	12-13	0.1699149778737521
1104	14-15	0.19331384336081925
1104	16-17	0.2400584405909072
1104	18-19	0.21936259934886237
1104	20-21	0.21572041790747676
1104	22-23	0.21744344931180848
1104	24-25	0.24350325462790323
1104	26-27	0.23022122042418403
1104	28-29	0.20920649624655852
1104	30-31	0.20644569406553614
1104	32-33	0.20238342042497948
1104	34-35	0.24410721961719872
1104	36-37	0.2922065172574051
1104	38-39	0.21980490281737985
1104	40-41	0.24397601122634427
1104	42-43	0.2788810246036988
1104	44-45	0.259553058170539
1104	46-47	0.2700958750912932
1104	48-49	0.19122906893785085
1104	50-51	0.1815439198933646
1104	52-53	0.1893027551147597
1104	54-55	0.1485385525176639
1104	56-57	0.15926298984151543
1104	58-59	0.14892157717564203
1104	60-61	0.13353680017475256
1104	62-63	0.1454411447799302
1104	64-65	0.22649092730637932
1104	66-67	0.24427385390352896
1104	68-69	0.2062392922032501
1104	70-71	0.19174392550353758
1104	72-73	0.17756295620483797
1104	74-75	0.1703174361675437
1104	76-77	0.13047436997976547
1104	78-79	0.15127549187401002
1104	80-81	0.18096226948063787
1104	82-83	0.1615611235115395
1104	84-85	0.11445663152158403
1104	86-87	0.15824585573389527
1104	88-89	0.12003064630422244
1104	90-91	0.15664207257231766
1104	92-93	0.15288548360594945
1104	94-95	0.10677048542090262
1104	96-97	0.08822490809021133
1104	98-99	0.05819908626757808
1104	100	0.046258491455013484
1105	1	-0.05162605754119198
1105	2	0.004590549871132055
1105	3	0.06370339628886512
1105	4	-0.09121059889459815
1105	5	-0.14893075784210907
1105	6	-0.10507972795694087
1105	7	-0.42424853892303105
1105	8	-0.3568615944084854
1105	9	-0.29123916211268863
1105	10-11	-0.2961161094799962
1105	12-13	-0.16769643847171523
1105	14-15	-0.12207113273731096
1105	16-17	-0.1309945308020204
1105	18-19	-0.1507608699784484
1105	20-21	-0.14573128628584442
1105	22-23	-0.11936481655718723
1105	24-25	-0.13555269759127242
1105	26-27	-0.11935445985682236
1105	28-29	-0.11049996106535076
1105	30-31	-0.1336987674866279
1105	32-33	-0.14949596861515602
1105	34-35	-0.13479938265406588
1105	36-37	-0.1802344797562867
1105	38-39	-0.2211121243809231
1105	40-41	-0.24582065241867923
1105	42-43	-0.1868403298702077
1105	44-45	-0.23348606542273842
1105	46-47	-0.2346274700847033
1105	48-49	-0.1901839064175661
1105	50-51	-0.14793303669697622
1105	52-53	-0.18971313416257374
1105	54-55	-0.19200926639663862
1105	56-57	-0.16877204930298717
1105	58-59	-0.1699355338222972
1105	60-61	-0.14410545985703038
1105	62-63	-0.1442688518897839
1105	64-65	-0.11866574279012099
1105	66-67	-0.06609847558628701
1105	68-69	-0.10832425420340996
1105	70-71	-0.1762840448813492
1105	72-73	-0.09183684138969284
1105	74-75	-0.13451449909456414
1105	76-77	-0.10328711115436562
1105	78-79	-0.12979812328380547
1105	80-81	-0.13398682300512377
1105	82-83	-0.15106536372810098
1105	84-85	-0.1113148414536056
1105	86-87	-0.15448428341613152
1105	88-89	-0.1477442923543819
1105	90-91	-0.14333757736478248
1105	92-93	-0.09005325862277402
1105	94-95	-0.06560224330449671
1105	96-97	-0.09855113518653269
1105	98-99	-0.12268508613249196
1105	100	-0.13043865550400469
1106	1	0.07300085250535204
1106	2	0.10122699160694992
1106	3	0.08830775995265583
1106	4	0.05675571233728505
1106	5	0.06346362148982365
1106	6	0.07647974083167952
1106	7	0.12331807980945086
1106	8	0.09610347349379111
1106	9	0.11273126541728118
1106	10-11	0.1468209180750648
1106	12-13	0.12614342796045008
1106	14-15	0.14737313283429643
1106	16-17	0.19225303716736875
1106	18-19	0.1600014774166496
1106	20-21	0.1809209648188812
1106	22-23	0.17766068618708175
1106	24-25	0.19432598964321102
1106	26-27	0.21718028609174667
1106	28-29	0.19138298975007473
1106	30-31	0.1949469582252661
1106	32-33	0.16783007746722944
1106	34-35	0.1875211383596067
1106	36-37	0.18036313557282568
1106	38-39	0.13870802464265353
1106	40-41	0.1292627195988203
1106	42-43	0.1459520696169463
1106	44-45	0.1373140823308816
1106	46-47	0.17076714009125027
1106	48-49	0.1992434597350723
1106	50-51	0.21765473819809955
1106	52-53	0.1814451048478034
1106	54-55	0.1876838124915423
1106	56-57	0.22792298052263504
1106	58-59	0.1675990598555117
1106	60-61	0.17379665947491674
1106	62-63	0.19348899188916846
1106	64-65	0.20111958515830963
1106	66-67	0.24293621249714192
1106	68-69	0.1868143130179618
1106	70-71	0.15619224008452903
1106	72-73	0.16678544067973178
1106	74-75	0.2146329948803185
1106	76-77	0.2311523567958389
1106	78-79	0.21595830617619782
1106	80-81	0.21962371146295823
1106	82-83	0.25279572253229077
1106	84-85	0.2370753899715936
1106	86-87	0.22464670512702156
1106	88-89	0.2371604308241828
1106	90-91	0.21935004785436973
1106	92-93	0.19099390344447187
1106	94-95	0.19743342453499224
1106	96-97	0.174279431184992
1106	98-99	0.17790821495216846
1106	100	0.18542025043207389
1107	1	-0.009606726618073935
1107	2	0.03407475586527653
1107	3	0.018584252581554495
1107	4	-0.056443813699964096
1107	5	-0.07844771707764409
1107	6	-0.02201678273028307
1107	7	-0.020425395180808437
1107	8	0.014971243168623971
1107	9	0.023483623352582583
1107	10-11	0.045574729071418574
1107	12-13	0.057212688300019465
1107	14-15	0.052770637922002095
1107	16-17	0.052596626103941446
1107	18-19	0.08574561025443472
1107	20-21	0.08485875592233327
1107	22-23	0.10480486528321364
1107	24-25	0.11965863387607811
1107	26-27	0.10871366784708414
1107	28-29	0.08889270194963217
1107	30-31	0.13870166223411928
1107	32-33	0.0949876959923941
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2216	36-37	-0.6423011930460305
2216	38-39	-0.6192287789337954
2216	40-41	-0.6245842731181099
2216	42-43	-0.5835581731299939
2216	44-45	-0.6017737933618434
2216	46-47	-0.5845158250464877
2216	48-49	-0.6267940608249134
2216	50-51	-0.5925567971644696
2216	52-53	-0.5696237083969891
2216	54-55	-0.6121832867245871
2216	56-57	-0.5892600071894236
2216	58-59	-0.7349673809904971
2216	60-61	-0.6517108039585224
2216	62-63	-0.6887311174063413
2216	64-65	-0.6474523935289938
2216	66-67	-0.5665907007333999
2216	68-69	-0.6383347763598195
2216	70-71	-0.6339949699688177
2216	72-73	-0.6253493099748653
2216	74-75	-0.5578881061318341
2216	76-77	-0.5372467356036168
2216	78-79	-0.49573530339232263
2216	80-81	-0.5659601960110052
2216	82-83	-0.5541133109629257
2216	84-85	-0.583059347935972
2216	86-87	-0.4829287787945873
2216	88-89	-0.4297585281344638
2216	90-91	-0.4452809399121236
2216	92-93	-0.6013755979709501
2216	94-95	-0.5887803057295962
2216	96-97	-0.6574002970320905
2216	98-99	-0.6509888551039715
2216	100	-0.6016856647875564
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	64.0
3	405.0
4	254.0
5	435.0
6	538.0
7	913.0
8	1658.0
9	2317.0
10	3191.0
11	4481.0
12	7066.0
13	10458.0
14	11315.0
15	14696.0
16	19465.0
17	30226.0
18	37990.0
19	21015.0
20	13249.0
21	12207.0
22	12307.0
23	12515.0
24	13859.0
25	16339.0
26	20117.0
27	25818.0
28	31809.0
29	39222.0
30	50383.0
31	62683.0
32	78877.0
33	101703.0
34	141280.0
35	230861.0
36	384278.0
37	849661.0
38	1671427.0
39	1065632.0
40	9347.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.93735885936509	21.977546849258957	15.379056860674966	23.70603743070098
2	12.70174409088913	22.73773168385209	41.07359991696706	23.48692430829172
3	14.85499117771515	29.39896687451597	32.31688768951945	23.429154258249437
4	10.28978859576425	19.199836804041894	40.613797244191936	29.896577356001924
5	11.450399506113799	39.10585120620288	35.86167912925611	13.582070158427213
6	26.855321721631732	40.804433319274956	19.06585568519026	13.274389273903054
7	23.61450289727011	34.97576177216206	24.560998359101816	16.848736971466018
8	24.15573383238248	35.67036409337132	21.915222189909464	18.258679884336736
9	25.413423110018023	16.320679528652445	22.48250071206718	35.78339664926236
10-11	22.407222187514282	27.923532268369588	30.167806340082485	19.501439204033645
12-13	23.927612857408324	26.08666840583378	30.188364972003335	19.79735376475456
14-15	21.34404151965415	27.043792480770197	28.61267557420957	22.99949042536608
16-17	19.57846421430797	30.443671244721372	28.828930426196408	21.14893411477425
18-19	19.572476263263063	28.572187045227594	32.25211229963068	19.603224391878662
20-21	21.029444551673123	27.617478110545964	32.00191574513763	19.351161592643283
22-23	20.623771470817893	27.65478070504601	31.99117934011117	19.73026848402493
24-25	20.309325914653044	27.770939862841836	31.978228584493724	19.941505638011396
26-27	20.12445424522069	27.81081924793325	31.96411441519159	20.10061209165447
28-29	20.077353131295833	27.774452768112962	32.11925404148354	20.02894005910766
30-31	20.343644943804527	27.858114848050413	31.860695703115837	19.937544505029226
32-33	20.06100392597251	27.79115866240479	32.11697415976134	20.030863251861362
34-35	20.301190870656995	27.77117957275569	31.830619308632315	20.097010247954998
36-37	20.397749518624416	27.868954061903707	31.57225005122397	20.161046368247902
38-39	20.328545803725024	27.929068007099104	31.565209901823888	20.177176287351976
40-41	20.307502068410752	27.74893036466905	31.586703087995033	20.356864478925164
42-43	20.26689043097217	27.889613911763266	31.458422383802425	20.385073273462144
44-45	20.52081267687624	27.95566019442377	31.214489398328617	20.309037730371372
46-47	20.56380451277148	28.066250922549735	30.83279731052113	20.53714725415765
48-49	20.663710949725665	28.190359907639834	30.445491294128168	20.700437848506333
50-51	20.76188432663591	28.296715826557318	30.158477648508864	20.78292219829791
52-53	20.650589274615182	28.41864305762528	30.134341758119366	20.796425909640167
54-55	20.734563290737515	28.629433549206613	29.999013979534688	20.636989180521187
56-57	20.655832334753253	28.34825057452245	30.11738152093086	20.87853556979344
58-59	20.5864641394005	28.184729617117675	30.28924007365233	20.93956616982949
60-61	20.748123244838094	28.402717461435216	30.11920954378939	20.7299497499373
62-63	20.607812958764374	28.22781000071656	30.334830254561773	20.829546785957298
64-65	20.61062729575548	28.09111505827973	30.40352203296527	20.894735612999522
66-67	20.591951669658837	28.167659194208888	30.286412999707284	20.95397613642499
68-69	20.819399186570845	28.157507014187257	30.120311786898448	20.90278201234345
70-71	20.786156840346553	28.29716317782926	29.94130984622715	20.97537013559704
72-73	20.740833960654932	28.31338329218443	30.00675740882363	20.939025338337004
74-75	20.812195456893505	28.288845987715682	29.876841430397505	21.022117124993315
76-77	20.82851841786876	28.24480725151967	29.838706860494813	21.08796747011675
78-79	20.878832335329342	28.33792415169661	29.83319361277445	20.950049900199602
80-81	20.73266624970197	28.298319975592896	29.881638114365494	21.087375660339642
82-83	20.716171084893347	28.272961458713407	29.95093417091084	21.05993328548241
84-85	20.727740989764392	28.228543632792785	29.809488423227855	21.234226954214964
86-87	20.71360187431319	28.342170739464894	29.74574996629274	21.19847741992917
88-89	20.615142176216274	28.2876877752007	29.794959261314418	21.30221078726861
90-91	20.64231367614933	28.206266449087035	29.719649822009785	21.431770052753844
92-93	20.61784717975086	28.28131512090432	29.748805967050917	21.3520317322939
94-95	20.49685374596894	28.097264563881218	29.847671823913167	21.558209866236673
96-97	20.569020908664577	28.197749820781876	29.672193760564586	21.56103550998896
98-99	20.40090871284277	28.201745189341953	29.720454931278166	21.676891166537107
100	20.41803271326046	28.217617335293454	29.621499053373373	21.742850898072714
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1879.0
1	1323.0
2	2433.5
3	3669.0
4	2106.0
5	1353.5
6	3291.0
7	8933.0
8	15728.5
9	16512.5
10	10529.0
11	5659.5
12	4897.5
13	5210.5
14	5745.5
15	6236.0
16	6831.5
17	8062.0
18	9390.5
19	10603.0
20	13322.5
21	16540.0
22	19464.5
23	24209.5
24	32079.0
25	39931.0
26	49067.0
27	62871.5
28	79184.5
29	97429.0
30	112875.0
31	126591.5
32	138314.5
33	146856.5
34	155895.0
35	163679.5
36	170510.0
37	174454.0
38	176750.5
39	179147.0
40	179971.5
41	178052.0
42	173293.0
43	169172.5
44	164679.0
45	158818.5
46	153217.5
47	146357.0
48	139428.5
49	134212.5
50	130480.0
51	125897.5
52	118953.0
53	112633.0
54	106893.5
55	99844.0
56	90503.5
57	80744.0
58	73442.5
59	67602.0
60	59502.5
61	49579.0
62	41625.0
63	34925.5
64	28080.0
65	21840.5
66	16864.0
67	12679.0
68	9280.5
69	6814.5
70	4836.0
71	3362.5
72	2462.5
73	1806.0
74	1304.0
75	893.0
76	528.0
77	329.5
78	214.5
79	127.5
80	59.5
81	17.0
82	8.5
83	8.5
84	6.5
85	4.0
86	5.5
87	4.0
88	2.0
89	1.5
90	2.5
91	4.0
92	3.5
93	3.5
94	5.5
95	9.5
96	12.5
97	16.0
98	10.5
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008582729831033994
2	1.9959836816358123E-5
3	4.1915657314352066E-4
4	0.0026546582965756307
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	9.979918408179063E-5
24-25	1.0977910248996967E-4
26-27	5.389155940416693E-4
28-29	0.0010079717592260853
30-31	0.0018662447423294848
32-33	0.004381184181190608
34-35	0.005608714145396633
36-37	0.003981987444863446
38-39	0.0027045578886165256
40-41	0.0035129312796790295
42-43	5.488955124498485E-4
44-45	5.089758388171322E-4
46-47	0.003363232503556344
48-49	0.0021756222129830356
50-51	9.680520855933692E-4
52-53	5.987951044907437E-5
54-55	4.590762467762368E-4
56-57	6.087750228989228E-4
58-59	7.185541253888925E-4
60-61	3.492971442862672E-4
62-63	0.0
64-65	0.0
66-67	8.08373391062504E-4
68-69	7.285340437970715E-4
70-71	0.00212572262094214
72-73	8.981926567361156E-5
74-75	0.0019361041711867383
76-77	4.2913649155169967E-4
78-79	0.0012175500457978455
80-81	8.882127383279365E-4
82-83	0.001137710698532413
84-85	0.0010079717592260853
86-87	2.4949796020447653E-4
88-89	0.001616746782125008
90-91	0.0018862045791458427
92-93	0.0029740156856373607
94-95	0.001107770943307876
96-97	0.002085802947309424
98-99	0.0023452808259220794
100	0.0033133329115154486
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5010061.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.620263705041005
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.72581464131557	39.89408724808863
2	16.44363547514225	18.291986827939585
3	5.427890148426655	9.057020442524543
4	2.358258860998909	5.246679189340359
5	1.2278695388068377	3.4147213771911704
6	0.7097365728252546	2.3685441204991626
7	0.4535011943499717	1.7656699214207534
8	0.2869138689452727	1.2766580041095714
9	0.2203289393359878	1.1029278336947663
>10	1.0579338286952717	10.217201185167545
>50	0.05881024450560729	2.237979135618874
>100	0.027616908530505392	2.644981483403263
>500	0.0010426305543046082	0.39546092194301474
>1k	5.033369970799612E-4	0.5671936216888179
>5k	3.595264264784634E-5	0.10078465143430239
>10k+	1.0785792794353902E-4	1.4181040359355137
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31584	0.6304114860078549	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21870	0.4365216311737522	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17462	0.34853867048724557	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5040	0.10059757755444496	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	1.9959836816358123E-5	0.0
12-13	0.0	1.9959836816358123E-5	0.0	1.9959836816358123E-5	0.0
14-15	0.0	3.991967363271625E-5	0.0	1.9959836816358123E-5	0.0
16-17	0.0	3.991967363271625E-5	0.0	1.9959836816358123E-5	0.0
18-19	0.0	3.991967363271625E-5	0.0	2.9939755224537183E-5	0.0
20-21	0.0	3.991967363271625E-5	0.0	7.98393472654325E-5	0.0
22-23	0.0	3.991967363271625E-5	0.0	2.894176338371928E-4	0.0
24-25	0.0	3.991967363271625E-5	0.0	5.289356756334903E-4	0.0
26-27	0.0	3.991967363271625E-5	0.0	0.0010179516776342643	0.0
28-29	0.0	3.991967363271625E-5	0.0	0.002275421397064826	0.0
30-31	0.0	3.991967363271625E-5	0.0	0.005478975206090305	0.0
32-33	0.0	3.991967363271625E-5	0.0	0.012285279560468425	0.0
34-35	0.0	3.991967363271625E-5	0.0	0.02257457543930104	0.0
36-37	0.0	3.991967363271625E-5	0.0	0.03804344897197859	0.0
38-39	0.0	3.991967363271625E-5	0.0	0.057673948480866793	0.0
40-41	0.0	3.991967363271625E-5	0.0	0.07964972881567708	0.0
42-43	0.0	3.991967363271625E-5	0.0	0.10110655339326208	0.0
44-45	0.0	3.991967363271625E-5	0.0	0.1286511281998363	0.0
46-47	0.0	3.991967363271625E-5	0.0	0.16243315201152242	0.0
48-49	0.0	3.991967363271625E-5	0.0	0.1996682275924385	0.0
50-51	0.0	3.991967363271625E-5	0.0	0.23824061224005058	0.0
52-53	0.0	5.987951044907437E-5	0.0	0.27551560749459936	0.0
54-55	0.0	7.98393472654325E-5	0.0	0.3171218873382979	0.0
56-57	0.0	9.979918408179062E-5	0.0	0.36914720200013534	0.0
58-59	0.0	9.979918408179062E-5	0.0	0.42762952387206465	0.0
60-61	0.0	9.979918408179062E-5	0.0	0.4923492947491059	0.0
62-63	0.0	9.979918408179062E-5	0.0	0.5594941857993345	0.0
64-65	0.0	9.979918408179062E-5	0.0	0.6333755217750842	0.0
66-67	0.0	9.979918408179062E-5	0.0	0.7182647077550552	0.0
68-69	0.0	9.979918408179062E-5	0.0	0.814560940475575	0.0
70-71	9.979918408179062E-6	9.979918408179062E-5	0.0	0.9234817699824414	0.0
72-73	1.9959836816358123E-5	9.979918408179062E-5	0.0	1.0403665743790347	0.0
74-75	1.9959836816358123E-5	9.979918408179062E-5	0.0	1.1617323621409001	0.0
76-77	1.9959836816358123E-5	1.0977910248996968E-4	0.0	1.296321541793603	0.0
78-79	1.9959836816358123E-5	1.297389393063278E-4	0.0	1.4486250766208235	0.0
80-81	1.9959836816358123E-5	1.3971885771450686E-4	0.0	1.6244612590545304	0.0
82-83	1.9959836816358123E-5	1.3971885771450686E-4	0.0	1.8156265961632003	0.0
84-85	1.9959836816358123E-5	1.3971885771450686E-4	0.0	2.0129894626033495	0.0
86-87	1.9959836816358123E-5	1.3971885771450686E-4	0.0	2.221230440108414	0.0
88	1.9959836816358123E-5	1.3971885771450686E-4	0.0	2.3926055990136645	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10680	0.0	60.338814	1
GTATCAA	22165	0.0	45.699356	1
ATCAACG	28560	0.0	35.20082	3
TCAACGC	28740	0.0	34.9473	4
TATCAAC	28955	0.0	34.753082	2
CAACGCA	29685	0.0	33.848923	5
AACGCAG	30515	0.0	32.974445	6
ACGCAGA	34700	0.0	28.889204	7
CGCAGAG	34900	0.0	28.65632	8
GCAGAGT	37890	0.0	26.04767	9
GTACATG	22255	0.0	25.365648	1
TACATGG	22635	0.0	24.335896	2
ACATGGG	22910	0.0	23.161627	3
AGAGTAC	35555	0.0	22.57014	10-11
GAGTACT	24200	0.0	22.343204	12-13
CAGAGTA	37240	0.0	21.290194	10-11
AGTACTT	25455	0.0	21.149311	12-13
CATGGGG	14155	0.0	20.752289	4
GTGGTAT	4385	0.0	20.687988	1
TGGTATC	4420	0.0	20.097393	2
>>END_MODULE