FastQCFastQC Report
Wed 25 May 2016
SRR2431364_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431364_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6021825
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA178280.29605642807620614No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72100.050.1295281
GTATCAA140850.044.4143941
TCAACGC172950.035.301044
ATCAACG173550.035.043593
TATCAAC185400.033.3361132
CAACGCA191650.031.8072745
AACGCAG199850.030.4316446
CGCAGAG211250.028.2777048
ACGCAGA215000.028.0030927
GTACATG337750.026.4538751
TACATGG335850.025.7776412
GCAGAGT230550.024.8504319
ACATGGG355500.023.519893
CATGGGG229400.020.734144
AGAGTAC209700.019.6896810-11
GTGGTAT35000.019.4716381
ATGCGGG8750.019.337038
CAGAGTA224600.019.22050310-11
ATGGGGG135900.018.8137255
CTGTCGC15750.018.799899
Produced by FastQC (version 0.11.5)