##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431364_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6021825
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.140747862981737	33.0	31.0	34.0	28.0	34.0
2	31.219594558128144	34.0	31.0	34.0	27.0	34.0
3	31.30894504572949	34.0	31.0	34.0	27.0	34.0
4	34.46340270599029	37.0	35.0	37.0	32.0	37.0
5	34.62701822122031	37.0	35.0	37.0	32.0	37.0
6	34.646034217201596	37.0	35.0	37.0	32.0	37.0
7	34.568140223271186	37.0	35.0	37.0	32.0	37.0
8	34.537332785326704	37.0	35.0	37.0	31.0	37.0
9	36.01846765723016	39.0	37.0	39.0	31.0	39.0
10-11	36.04454837196365	39.0	37.0	39.0	31.0	39.0
12-13	35.98808874386087	39.0	37.0	39.0	31.0	39.0
14-15	37.3783738318533	41.0	38.0	41.0	31.0	41.0
16-17	37.41506719308515	41.0	38.0	41.0	31.0	41.0
18-19	37.47313787763676	41.0	38.5	41.0	31.0	41.0
20-21	37.54868216861168	41.0	38.5	41.0	31.0	41.0
22-23	37.5714942729156	41.0	38.0	41.0	31.0	41.0
24-25	37.60684809007236	41.0	38.0	41.0	31.0	41.0
26-27	37.61611587849198	41.0	38.0	41.0	31.0	41.0
28-29	37.58938785168948	41.0	38.0	41.0	30.5	41.0
30-31	37.52012861549447	40.0	38.0	41.0	30.0	41.0
32-33	37.42810916956238	40.0	38.0	41.0	30.0	41.0
34-35	37.354567178554674	40.0	38.0	41.0	30.0	41.0
36-37	37.250636227389535	40.0	38.0	41.0	30.0	41.0
38-39	37.13938432285893	40.0	38.0	41.0	30.0	41.0
40-41	37.039528714301724	40.0	38.0	41.0	30.0	41.0
42-43	36.91197452599503	40.0	37.5	41.0	30.0	41.0
44-45	36.78200836125261	40.0	37.0	41.0	30.0	41.0
46-47	36.6476975003425	40.0	37.0	41.0	29.0	41.0
48-49	36.501388532546194	40.0	36.5	41.0	29.0	41.0
50-51	36.34743487231861	40.0	36.0	41.0	29.0	41.0
52-53	36.14489984348599	40.0	35.5	41.0	29.0	41.0
54-55	35.95435943422467	40.0	35.0	41.0	28.5	41.0
56-57	35.72560859872215	39.0	35.0	41.0	28.0	41.0
58-59	35.47371884769152	39.0	35.0	41.0	28.0	41.0
60-61	35.268102444026525	39.0	35.0	41.0	27.5	41.0
62-63	35.074752919588335	38.5	35.0	41.0	27.0	41.0
64-65	34.80617902380092	38.0	34.5	40.0	26.5	41.0
66-67	34.49617948047311	37.0	34.0	40.0	26.0	41.0
68-69	34.18057847911555	37.0	34.0	40.0	26.0	41.0
70-71	33.92934666816123	36.0	34.0	39.5	26.0	41.0
72-73	33.6828225662486	36.0	34.0	39.0	26.0	41.0
74-75	33.34878321771224	35.5	34.0	39.0	26.0	41.0
76-77	33.005884511755156	35.0	33.5	38.5	25.0	40.0
78-79	32.67492828170862	35.0	33.0	37.0	25.0	39.5
80-81	32.34660223437247	35.0	33.0	37.0	24.5	39.0
82-83	32.03098122246993	35.0	33.0	36.0	24.0	39.0
84-85	31.752127552693743	35.0	33.0	36.0	23.5	37.5
86-87	31.495129466565366	35.0	33.0	36.0	23.0	37.0
88-89	31.255666845183974	35.0	32.0	35.0	21.0	37.0
90-91	31.0477810298373	35.0	32.0	35.0	20.0	36.0
92-93	30.84582464618284	35.0	32.0	35.0	19.5	36.0
94-95	30.635283240545846	35.0	32.0	35.0	17.5	36.0
96-97	30.45649964919273	35.0	32.0	35.0	11.0	36.0
98-99	30.33596957732913	35.0	32.0	35.0	2.0	35.0
100	30.289672815134946	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.48940028687811576
1101	2	0.5634159561672405
1101	3	0.5046651513204452
1101	4	0.5420913777988332
1101	5	0.4806806579587146
1101	6	0.47705886926118524
1101	7	0.4727871382942084
1101	8	0.4936561548977494
1101	9	0.5652445510563453
1101	10-11	0.5492579506974025
1101	12-13	0.558996293131031
1101	14-15	0.6468754465871172
1101	16-17	0.6518530290419378
1101	18-19	0.6329461838793833
1101	20-21	0.5722796581575125
1101	22-23	0.6357910647295526
1101	24-25	0.6409815239821484
1101	26-27	0.6232879292232667
1101	28-29	0.7063981176188889
1101	30-31	0.7580920382140164
1101	32-33	0.7322710429632906
1101	34-35	0.7905649867432913
1101	36-37	0.7448418192763313
1101	38-39	0.7386505639465071
1101	40-41	0.757119588148278
1101	42-43	0.7591463202163808
1101	44-45	0.739582137102893
1101	46-47	0.7203553795247899
1101	48-49	0.6913552128812626
1101	50-51	0.6420126841443476
1101	52-53	0.6615277596727651
1101	54-55	0.6518634770242002
1101	56-57	0.6604869512603102
1101	58-59	0.7012489875023746
1101	60-61	0.7391845978791451
1101	62-63	0.691556281673634
1101	64-65	0.6980931437988929
1101	66-67	0.6218324137953246
1101	68-69	0.6615170640343351
1101	70-71	0.6467074809122977
1101	72-73	0.6711364397272916
1101	74-75	0.681233407021935
1101	76-77	0.6772356327714988
1101	78-79	0.6604964478640554
1101	80-81	0.6889237770481031
1101	82-83	0.7253664076070407
1101	84-85	0.7031125090475463
1101	86-87	0.6866563806876158
1101	88-89	0.6623335110046007
1101	90-91	0.6991131026656703
1101	92-93	0.7443939492065965
1101	94-95	0.7526548239441553
1101	96-97	0.7670785111989886
1101	98-99	0.7974158305735912
1101	100	0.8069053112993849
1102	1	0.25133923444010975
1102	2	0.26855137991881506
1102	3	0.3188094890574611
1102	4	0.2926281249295428
1102	5	0.24193093896604978
1102	6	0.24198113005381572
1102	7	0.24752654799119966
1102	8	0.24132209764499635
1102	9	0.28625028892282245
1102	10-11	0.30434319313066993
1102	12-13	0.3203047561956396
1102	14-15	0.3704263811441848
1102	16-17	0.3324250967767526
1102	18-19	0.28592719030442026
1102	20-21	0.26402792716010737
1102	22-23	0.2698504823647241
1102	24-25	0.35262005654085016
1102	26-27	0.38618093542788756
1102	28-29	0.4001652404606517
1102	30-31	0.4377416943912351
1102	32-33	0.3957640078558313
1102	34-35	0.4441530141977097
1102	36-37	0.43794544071539576
1102	38-39	0.40514048378504697
1102	40-41	0.4287337585238973
1102	42-43	0.42321979234885276
1102	44-45	0.43747450196330817
1102	46-47	0.42713085238145965
1102	48-49	0.4092230667142047
1102	50-51	0.43211174742876324
1102	52-53	0.3938960084870331
1102	54-55	0.40194537321247736
1102	56-57	0.42384297084161915
1102	58-59	0.37405819191513245
1102	60-61	0.39885471211043466
1102	62-63	0.4245008929623424
1102	64-65	0.37771942123927005
1102	66-67	0.36922997768152044
1102	68-69	0.35299033102420907
1102	70-71	0.36248265182950945
1102	72-73	0.36045965375102185
1102	74-75	0.3158083995913685
1102	76-77	0.32443091374345556
1102	78-79	0.2580769300659327
1102	80-81	0.2752660418403252
1102	82-83	0.2843623499592951
1102	84-85	0.3370501256526204
1102	86-87	0.3146174925913563
1102	88-89	0.30206433058685533
1102	90-91	0.2822333171473268
1102	92-93	0.30734621496630865
1102	94-95	0.31004034485058796
1102	96-97	0.28880723597298896
1102	98-99	0.27136628310078237
1102	100	0.30530149888336666
1103	1	0.18826419585444398
1103	2	0.21094556137081355
1103	3	0.2752756384625741
1103	4	0.23477481174342074
1103	5	0.2511741303408286
1103	6	0.22954677774765742
1103	7	0.3086001885204652
1103	8	0.3023277625207115
1103	9	0.3153210701360649
1103	10-11	0.353970479557276
1103	12-13	0.320906453392503
1103	14-15	0.36438051567409246
1103	16-17	0.3779923319127434
1103	18-19	0.35481461611428244
1103	20-21	0.26756331892834595
1103	22-23	0.2753444112795478
1103	24-25	0.33804239982887196
1103	26-27	0.35070210181060446
1103	28-29	0.3716616227969354
1103	30-31	0.34775497280607226
1103	32-33	0.30476415932803036
1103	34-35	0.3704868943452908
1103	36-37	0.3738066118422836
1103	38-39	0.3683132284506172
1103	40-41	0.3535066688278192
1103	42-43	0.28467693620446965
1103	44-45	0.30035511786388014
1103	46-47	0.29258416552838895
1103	48-49	0.3560989248508548
1103	50-51	0.2608436174563451
1103	52-53	0.3040314387454117
1103	54-55	0.26055030371041
1103	56-57	0.2684280522527871
1103	58-59	0.25328346064183904
1103	60-61	0.286874661656185
1103	62-63	0.22057436617662063
1103	64-65	0.3275675732580865
1103	66-67	0.331680517766074
1103	68-69	0.3193028105270699
1103	70-71	0.2910524859251069
1103	72-73	0.26359140841172746
1103	74-75	0.2911809966530683
1103	76-77	0.3097031913643633
1103	78-79	0.2263366454305853
1103	80-81	0.28854054495361936
1103	82-83	0.28063033682875727
1103	84-85	0.26009937659575755
1103	86-87	0.2955751636046742
1103	88-89	0.303106553964259
1103	90-91	0.2693377184722294
1103	92-93	0.350963254213589
1103	94-95	0.3401972600059828
1103	96-97	0.27843402808209916
1103	98-99	0.2997775801141813
1103	100	0.2884036359206341
1104	1	0.21572716592717
1104	2	0.21550681661620885
1104	3	0.22660389560136807
1104	4	0.1323737009849424
1104	5	0.24779148512966032
1104	6	0.2190380863640442
1104	7	0.18109587836509178
1104	8	0.22186402107143977
1104	9	0.2556456831046674
1104	10-11	0.2433945034205962
1104	12-13	0.22882082888160937
1104	14-15	0.21840364741591856
1104	16-17	0.2781214439287609
1104	18-19	0.259837000933004
1104	20-21	0.2502829875944599
1104	22-23	0.24400884995691996
1104	24-25	0.26890412442516265
1104	26-27	0.32765727631621644
1104	28-29	0.35294677048729284
1104	30-31	0.3643591570140643
1104	32-33	0.3665501469846859
1104	34-35	0.35496816227300343
1104	36-37	0.38137549878838684
1104	38-39	0.30612798976565614
1104	40-41	0.3447141381074701
1104	42-43	0.3777905162225679
1104	44-45	0.34326244753624024
1104	46-47	0.33021773655269016
1104	48-49	0.29187960376235367
1104	50-51	0.3102363185113788
1104	52-53	0.35392627882212935
1104	54-55	0.3056922893436891
1104	56-57	0.29085840403809016
1104	58-59	0.2921008576249946
1104	60-61	0.3706137203770652
1104	62-63	0.3100910769532703
1104	64-65	0.3084980448207162
1104	66-67	0.3072755032827388
1104	68-69	0.2950684775621184
1104	70-71	0.2689095835441577
1104	72-73	0.21860930132743306
1104	74-75	0.2653560158504291
1104	76-77	0.24246101590246383
1104	78-79	0.2901154131949042
1104	80-81	0.3440745148132933
1104	82-83	0.3079999600690968
1104	84-85	0.2642546196789688
1104	86-87	0.26221290854277157
1104	88-89	0.34744985535467166
1104	90-91	0.323791081000703
1104	92-93	0.32259862418533203
1104	94-95	0.36210941338974223
1104	96-97	0.3863506856225847
1104	98-99	0.3581825689834801
1104	100	0.378191547787889
1105	1	0.35715928497418403
1105	2	0.4053193666538242
1105	3	0.3797072019846226
1105	4	0.40711491842857583
1105	5	0.3549971103182017
1105	6	0.32824312773352915
1105	7	0.3486986372044001
1105	8	0.43685389672253194
1105	9	0.41023264820920957
1105	10-11	0.4580193904558385
1105	12-13	0.49850846263336734
1105	14-15	0.5334065875304219
1105	16-17	0.5623619732030747
1105	18-19	0.5099759651106339
1105	20-21	0.49039183678718246
1105	22-23	0.5040588586182011
1105	24-25	0.5407930944949584
1105	26-27	0.5731290918044394
1105	28-29	0.6107076909697327
1105	30-31	0.5855764381882977
1105	32-33	0.6462784799165746
1105	34-35	0.6261308775474674
1105	36-37	0.6007333450184333
1105	38-39	0.6581946118571054
1105	40-41	0.6529606595686488
1105	42-43	0.6058446044028898
1105	44-45	0.5571356193961989
1105	46-47	0.5325963028806626
1105	48-49	0.5453398083951839
1105	50-51	0.5625451918802753
1105	52-53	0.5696172404455666
1105	54-55	0.5742954261764126
1105	56-57	0.5184123228848563
1105	58-59	0.5335264593571836
1105	60-61	0.6177024496723504
1105	62-63	0.6452936112584737
1105	64-65	0.6700928632218321
1105	66-67	0.6158444189238637
1105	68-69	0.6253974544199039
1105	70-71	0.6349991387728124
1105	72-73	0.6147180887372059
1105	74-75	0.5634563555400405
1105	76-77	0.5473264667106719
1105	78-79	0.6059151784468142
1105	80-81	0.5487915170285618
1105	82-83	0.5547980816721036
1105	84-85	0.552216046179506
1105	86-87	0.4909522165731772
1105	88-89	0.5481638145832974
1105	90-91	0.535346497175464
1105	92-93	0.5299259967747432
1105	94-95	0.5887015401099909
1105	96-97	0.5797663913424138
1105	98-99	0.6107314547436822
1105	100	0.6306815458251016
1106	1	0.14795285901109878
1106	2	0.19593910567696682
1106	3	0.19660205099443218
1106	4	0.23583221842733337
1106	5	0.17923460673507208
1106	6	0.18522833405979355
1106	7	0.1482786813236956
1106	8	0.20002370616207088
1106	9	0.22339478039261706
1106	10-11	0.16324785114105822
1106	12-13	0.23367432127713528
1106	14-15	0.18057967475949965
1106	16-17	0.2239617588381364
1106	18-19	0.21001703095307533
1106	20-21	0.21128530239531784
1106	22-23	0.20728122485820677
1106	24-25	0.18308222544452235
1106	26-27	0.2492890180230205
1106	28-29	0.20638980769891901
1106	30-31	0.22321474719785783
1106	32-33	0.22728523490492591
1106	34-35	0.2365285369813961
1106	36-37	0.231212799634136
1106	38-39	0.23195363824144266
1106	40-41	0.23113476877014705
1106	42-43	0.18365076614871612
1106	44-45	0.20416487024057517
1106	46-47	0.18264099547884172
1106	48-49	0.145353679801147
1106	50-51	0.14293546070886975
1106	52-53	0.17611623716777558
1106	54-55	0.18593055710105233
1106	56-57	0.23954121603888723
1106	58-59	0.23362495506364667
1106	60-61	0.17867895677007084
1106	62-63	0.24345737610511264
1106	64-65	0.26837581248464204
1106	66-67	0.2690542348442051
1106	68-69	0.292880623032886
1106	70-71	0.2593398170135188
1106	72-73	0.2344169558434004
1106	74-75	0.30945963332959536
1106	76-77	0.2968168140566547
1106	78-79	0.3009406155892762
1106	80-81	0.3182857403918433
1106	82-83	0.3183791353007237
1106	84-85	0.3108959943331051
1106	86-87	0.3007315203856322
1106	88-89	0.30950213730224263
1106	90-91	0.2744330283269285
1106	92-93	0.3265741017637147
1106	94-95	0.2994529141643554
1106	96-97	0.2771610347114297
1106	98-99	0.32252330887581593
1106	100	0.2720142726219663
1107	1	0.054511756254207455
1107	2	0.06723317418156327
1107	3	0.09912753136201857
1107	4	0.07575285334796433
1107	5	0.06875925168076691
1107	6	0.10894846772729494
1107	7	0.10308319260189336
1107	8	0.13136372287051046
1107	9	0.11509068682536139
1107	10-11	0.08079505247773255
1107	12-13	0.12032386179509302
1107	14-15	0.146108496814648
1107	16-17	0.1595052007763158
1107	18-19	0.15374510112851425
1107	20-21	0.16087886964678688
1107	22-23	0.202890665125544
1107	24-25	0.18128147356482316
1107	26-27	0.13791558443905672
1107	28-29	0.2025355888183782
1107	30-31	0.1842235191276842
1107	32-33	0.23272884392747528
1107	34-35	0.223517258785904
1107	36-37	0.24782307532336034
1107	38-39	0.2263099456766966
1107	40-41	0.18858171948025415
1107	42-43	0.16489721560569848
1107	44-45	0.20661599806012276
1107	46-47	0.19615102054149958
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2216	90-91	-1.2745751122544533
2216	92-93	-1.3960931615060446
2216	94-95	-1.4638498737686696
2216	96-97	-1.5293153189589468
2216	98-99	-1.5986764785843306
2216	100	-1.5621513457520528
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	110202.0
3	21285.0
4	14115.0
5	12020.0
6	9046.0
7	10333.0
8	12897.0
9	13484.0
10	13424.0
11	14813.0
12	17972.0
13	18701.0
14	19170.0
15	17738.0
16	16243.0
17	15906.0
18	15748.0
19	15230.0
20	15392.0
21	16997.0
22	21658.0
23	35497.0
24	57450.0
25	69134.0
26	63583.0
27	47014.0
28	46870.0
29	55909.0
30	66870.0
31	79778.0
32	104328.0
33	141400.0
34	206049.0
35	324888.0
36	600220.0
37	1178286.0
38	1689947.0
39	815792.0
40	16436.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.56577554404369	21.492525912081177	13.635795247438267	22.305903296436867
2	15.267458132681128	22.423632319858065	39.737514615595515	22.571394931865292
3	17.3248338045084	29.309176090168027	30.01329829633015	23.35269180899342
4	11.932001322523096	18.729643830448023	39.73941113845425	29.59894370857463
5	12.858464953220977	39.957766942004106	33.98193537073408	13.201832734040838
6	27.975904314721866	40.44720661925579	18.648067653908907	12.928821412113436
7	24.525372291622556	34.973301283248844	23.8279923445135	16.673334080615096
8	23.653460537295587	36.81908723684265	21.664777704433458	17.862674521428303
9	25.091081192163507	17.8087373844308	21.78768064498719	35.3125007784185
10-11	23.110095361456036	28.71226746044596	28.30048199673687	19.877155181361132
12-13	23.51219937477426	27.421329912443486	28.3572754106936	20.709195302088652
14-15	21.815105885674193	27.690010918616863	26.160250422421775	24.33463277328717
16-17	21.172509994893574	31.037642243007724	26.301561403727273	21.488286358371425
18-19	20.76561507516409	29.7382521079573	28.41258256425585	21.08355025262275
20-21	23.8245382421442	28.565227318960613	26.823878143253914	20.786356295641273
22-23	22.4720744956886	29.115550518322937	27.57471364578014	20.837661340208324
24-25	22.09023842439792	28.58114442050375	27.350529117003564	21.978088038094764
26-27	21.805623710420015	29.164082317237717	27.429898743321168	21.600395229021103
28-29	21.470916208956588	28.827780614680766	27.87764506607216	21.823658110290488
30-31	22.667937045663066	29.406309549015457	26.669938100160667	21.255815305160812
32-33	21.161607984290477	29.313331091488042	28.151291344401407	21.37376957982007
34-35	21.956142863666745	28.121865049216808	27.95469811892574	21.967293968190706
36-37	22.306277581962277	29.234285287267564	27.09806412507836	21.361373005691796
38-39	21.638132958031825	28.9717569009395	27.62664142514935	21.763468715879323
40-41	21.990717099882513	28.72160017934762	27.374126614439977	21.913556106329892
42-43	21.247935634130847	28.898631228904858	28.126788805719194	21.726644331245097
44-45	21.827186940836043	28.332382624868707	27.680088677435826	22.160341756859424
46-47	22.069422475744478	29.176744591548243	27.421645431409914	21.332187501297366
48-49	21.487373013994926	28.646216055763823	27.848393136632165	22.01801779360908
50-51	22.216794742457644	28.664333486941253	26.940661676485117	22.178210094115986
52-53	21.593428902367638	28.899569482673442	27.67318047269723	21.83382114226169
54-55	22.093841983119734	28.672337704931643	27.24114367322199	21.992676638726632
56-57	21.988450345202658	28.99820237220444	27.155023601649003	21.8583236809439
58-59	21.775159523898484	28.238532338618278	27.699875037883032	22.286433099600202
60-61	22.683015531005964	28.82642720437741	26.73982555122409	21.750731713392536
62-63	21.641047356905922	28.950243489307642	27.71105105179908	21.697658101987354
64-65	22.135726650264107	28.156991967882156	27.425311666365538	22.2819697154882
66-67	21.90712948317163	28.760525256047792	27.15650986204348	22.175835398737092
68-69	22.02719275302753	28.75671411905859	27.34222598630814	21.873867141605743
70-71	22.567402739202816	28.55831081109132	26.8476915220884	22.026594927617456
72-73	21.755331647797803	28.831915573767088	27.71759391878708	21.695158859648032
74-75	21.965608432659533	28.241787165850884	27.440302566082543	22.35230183540704
76-77	22.138288641732366	28.90853686382451	26.923648561690182	22.029525932752943
78-79	21.878849020023	28.66792874253237	27.519215520211898	21.93400671723273
80-81	22.223382815572158	28.648977452678793	27.140057563954045	21.987582167795004
82-83	21.486720702405933	29.093394900575692	27.838881520311894	21.58100287670648
84-85	21.74977799384486	28.232673244719074	27.438300140697308	22.57924862073876
86-87	22.11549072102135	29.070761994444645	27.097965518864804	21.715781765669202
88-89	21.387165405700557	28.643469938710215	27.537649837722768	22.431714817866464
90-91	21.869316626358124	28.539684919972036	27.00008876265605	22.590909691013792
92-93	21.391717288349003	29.049581667936103	27.626655644329688	21.93204539938521
94-95	21.59155769027339	28.132408082279525	27.528186728137506	22.747847499309582
96-97	21.541928347874723	29.055878474578567	27.27668567092838	22.125507506618334
98-99	21.061575560751955	28.687091383466868	27.64523287561584	22.60610018016534
100	21.824740266674105	28.533325627813078	26.94057466242265	22.701359443090166
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	14392.0
1	10235.5
2	5038.0
3	3404.5
4	2421.0
5	1862.5
6	1579.5
7	1555.0
8	1710.0
9	1708.0
10	1541.0
11	1385.0
12	1351.0
13	1410.0
14	1578.5
15	1740.5
16	2010.5
17	2612.5
18	3372.5
19	4479.0
20	6374.5
21	8883.5
22	12328.0
23	17938.0
24	24781.0
25	31976.0
26	41913.0
27	55824.5
28	73406.5
29	90347.0
30	103008.0
31	116591.5
32	131139.5
33	143172.5
34	155965.0
35	171991.5
36	186209.5
37	196982.0
38	209615.0
39	220396.0
40	226745.5
41	223924.0
42	215839.0
43	210761.5
44	201964.0
45	191270.5
46	186398.0
47	181153.5
48	176343.5
49	178671.0
50	180497.5
51	175580.5
52	167835.0
53	160567.0
54	157884.0
55	156954.5
56	144101.5
57	127553.5
58	117704.0
59	109715.5
60	97279.0
61	80524.0
62	64769.5
63	51296.0
64	41274.0
65	34229.5
66	26639.0
67	19276.5
68	14441.0
69	10686.0
70	7984.0
71	6563.0
72	4945.0
73	3360.0
74	2504.0
75	1872.0
76	1289.5
77	913.5
78	693.0
79	501.5
80	349.0
81	271.5
82	227.5
83	191.0
84	162.5
85	143.5
86	121.0
87	107.5
88	88.5
89	74.0
90	77.5
91	69.0
92	55.5
93	43.5
94	36.5
95	33.5
96	32.5
97	28.0
98	25.0
99	30.5
100	69.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	3.3212522781714846E-4
2	6.642504556342969E-5
3	1.660626139085742E-5
4	3.321252278171484E-5
5	3.321252278171484E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	1.660626139085742E-5
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	1.660626139085742E-5
82-83	1.3285009112685937E-4
84-85	5.397034952028662E-4
86-87	0.0011375289052737334
88-89	0.0030804614880040517
90-91	0.0024660298165423275
92-93	0.0018848106678623178
94-95	0.0017934762302126016
96-97	0.001751960576735458
98-99	0.002225239026374895
100	0.002789851913664047
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6021825.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.38544648030214
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.26953734430305	34.70292475712671
2	15.38079249239429	15.191746089155425
3	5.928126378856958	8.78289504034523
4	2.9145149324086757	5.757384848420402
5	1.60405704395809	3.9608536647871966
6	0.9907859252023585	2.935824316951063
7	0.6348530686242175	2.194675157037782
8	0.439950344201895	1.7381715342058555
9	0.3206404489025955	1.4251474555829269
>10	1.377879594857784	11.56145162039251
>50	0.07575206991144214	2.5887755374733055
>100	0.05567245468869477	5.521131984558503
>500	0.005283961507898306	1.7561773855886438
>1k	0.0021202848667263184	1.5865244914849714
>5k	0.0	0.0
>10k+	3.36553153231191E-5	0.2963161168894581
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	17828	0.29605642807620614	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	1.660626139085742E-5
3	0.0	0.0	0.0	0.0	1.660626139085742E-5
4	0.0	0.0	0.0	0.0	1.660626139085742E-5
5	0.0	0.0	0.0	1.660626139085742E-5	1.660626139085742E-5
6	0.0	0.0	0.0	1.660626139085742E-5	1.660626139085742E-5
7	0.0	0.0	0.0	1.660626139085742E-5	1.660626139085742E-5
8	0.0	0.0	0.0	1.660626139085742E-5	1.660626139085742E-5
9	0.0	0.0	0.0	1.660626139085742E-5	1.660626139085742E-5
10-11	0.0	0.0	0.0	2.4909392086286132E-5	3.321252278171484E-5
12-13	0.0	0.0	0.0	3.321252278171484E-5	3.321252278171484E-5
14-15	0.0	0.0	0.0	6.642504556342969E-5	3.321252278171484E-5
16-17	0.0	0.0	0.0	6.642504556342969E-5	3.321252278171484E-5
18-19	0.0	0.0	0.0	1.1624382973600196E-4	4.151565347714356E-5
20-21	0.0	0.0	0.0	2.6570018225371874E-4	4.981878417257227E-5
22-23	0.0	0.0	0.0	5.895222793754385E-4	8.303130695428711E-5
24-25	0.0	0.0	0.0	0.0010461944676240175	1.3285009112685937E-4
26-27	0.0	0.0	0.0	0.0014862603944817393	1.3285009112685937E-4
28-29	0.0	0.0	0.0	0.003205008448435483	1.494563525177168E-4
30-31	0.0	0.0	0.0	0.007223723705022979	1.6606261390857423E-4
32-33	0.0	0.0	0.0	0.015418913701411118	1.6606261390857423E-4
34-35	0.0	0.0	0.0	0.026827415276930167	1.6606261390857423E-4
36-37	0.0	0.0	0.0	0.04398168329368589	1.6606261390857423E-4
38-39	0.0	0.0	0.0	0.06929792878404803	1.6606261390857423E-4
40-41	0.0	0.0	0.0	0.09791882029119078	1.6606261390857423E-4
42-43	0.0	0.0	0.0	0.12418992581152724	1.6606261390857423E-4
44-45	0.0	0.0	0.0	0.15520211895895347	1.7436574460400294E-4
46-47	0.0	0.0	0.0	0.19212614116152493	1.8266887529943166E-4
48-49	0.0	0.0	0.0	0.23347573202475994	1.8266887529943166E-4
50-51	0.0	0.0	0.0	0.2796162292992573	1.9927513669028908E-4
52-53	0.0	0.0	0.0	0.3245859851456992	2.1588139808114649E-4
54-55	0.0	0.0	0.0	0.3739231877379366	2.1588139808114649E-4
56-57	0.0	0.0	0.0	0.43259310923183586	2.3248765947200391E-4
58-59	0.0	0.0	0.0	0.4992423393240421	2.3248765947200391E-4
60-61	0.0	0.0	0.0	0.5743607627255857	2.490939208628613E-4
62-63	0.0	0.0	1.660626139085742E-5	0.6511481153969103	2.490939208628613E-4
64-65	0.0	0.0	1.660626139085742E-5	0.7330003777924466	2.740033129491475E-4
66-67	0.0	0.0	1.660626139085742E-5	0.8246337281471978	2.989127050354336E-4
68-69	0.0	0.0	1.660626139085742E-5	0.9328152179779385	2.989127050354336E-4
70-71	0.0	0.0	1.660626139085742E-5	1.0571546001419834	2.989127050354336E-4
72-73	0.0	0.0	1.660626139085742E-5	1.1875635708443868	2.989127050354336E-4
74-75	0.0	0.0	1.660626139085742E-5	1.3245237116654835	2.989127050354336E-4
76-77	0.0	0.0	1.660626139085742E-5	1.4695794049146231	2.989127050354336E-4
78-79	0.0	0.0	1.660626139085742E-5	1.6359492346589282	2.989127050354336E-4
80-81	0.0	0.0	1.660626139085742E-5	1.8277432505926359	3.0721583573086234E-4
82-83	0.0	0.0	1.660626139085742E-5	2.0341590796809936	3.1551896642629103E-4
84-85	0.0	0.0	1.660626139085742E-5	2.2490441020786887	3.1551896642629103E-4
86-87	0.0	0.0	1.660626139085742E-5	2.476915220884034	3.1551896642629103E-4
88	0.0	0.0	1.660626139085742E-5	2.6616515757266277	3.1551896642629103E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7210	0.0	50.129528	1
GTATCAA	14085	0.0	44.414394	1
TCAACGC	17295	0.0	35.30104	4
ATCAACG	17355	0.0	35.04359	3
TATCAAC	18540	0.0	33.336113	2
CAACGCA	19165	0.0	31.807274	5
AACGCAG	19985	0.0	30.431644	6
CGCAGAG	21125	0.0	28.277704	8
ACGCAGA	21500	0.0	28.003092	7
GTACATG	33775	0.0	26.453875	1
TACATGG	33585	0.0	25.777641	2
GCAGAGT	23055	0.0	24.850431	9
ACATGGG	35550	0.0	23.51989	3
CATGGGG	22940	0.0	20.73414	4
AGAGTAC	20970	0.0	19.68968	10-11
GTGGTAT	3500	0.0	19.471638	1
ATGCGGG	875	0.0	19.33703	8
CAGAGTA	22460	0.0	19.220503	10-11
ATGGGGG	13590	0.0	18.813725	5
CTGTCGC	1575	0.0	18.79989	9
>>END_MODULE