FastQCFastQC Report
Wed 25 May 2016
SRR2431364_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431364_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6021825
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT370210.6147804029509326No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT245450.40760068583859543No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191210.31752832405458475No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA124750.065.7122341
GTATCAA287350.051.9692341
TATCAAC397450.037.344812
ATCAACG400350.036.992123
TCAACGC404100.036.6485334
CAACGCA420950.035.1918335
AACGCAG433150.034.2440346
CGCAGAG475050.030.9466518
ACGCAGA476800.030.9316397
GTGGTAT37750.030.2572611
GCAGAGT507950.028.6368949
TGGTATC39450.028.4742032
GTACATG319350.025.9198651
TACATGG315350.025.5904642
ACATGGG325200.024.1794283
AGAGTAC487850.023.97865510-11
AGTACTT337750.023.39848312-13
GAGTACT327050.022.79880712-13
CAGAGTA502350.022.11705610-11
CATGGGG216000.020.8453314
Produced by FastQC (version 0.11.5)