##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431364_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6021825
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.79128569827253	34.0	31.0	34.0	31.0	34.0
2	32.902284772473465	34.0	31.0	34.0	31.0	34.0
3	32.980666990488764	34.0	31.0	34.0	31.0	34.0
4	36.368019661813484	37.0	37.0	37.0	35.0	37.0
5	36.32958081644684	37.0	37.0	37.0	35.0	37.0
6	36.364600266530495	37.0	37.0	37.0	35.0	37.0
7	36.23253133393946	37.0	37.0	37.0	35.0	37.0
8	36.28681471148697	37.0	37.0	37.0	35.0	37.0
9	38.10628472265468	39.0	39.0	39.0	37.0	39.0
10-11	38.101970000788796	39.0	39.0	39.0	36.0	39.0
12-13	38.07343911521839	39.0	39.0	39.0	35.0	39.0
14-15	39.54304633562084	41.0	39.5	41.0	37.0	41.0
16-17	39.562122280205756	41.0	39.5	41.0	37.0	41.0
18-19	39.65048320069082	41.0	40.0	41.0	37.0	41.0
20-21	39.65759566244452	41.0	40.0	41.0	37.0	41.0
22-23	39.577710660804655	41.0	40.0	41.0	37.0	41.0
24-25	39.54677261461434	41.0	40.0	41.0	36.0	41.0
26-27	39.45692086701291	41.0	39.5	41.0	36.0	41.0
28-29	39.36466552913776	41.0	39.0	41.0	36.0	41.0
30-31	39.182186629468646	41.0	39.0	41.0	35.5	41.0
32-33	38.95470251958501	41.0	39.0	41.0	35.0	41.0
34-35	38.845921294624134	41.0	38.5	41.0	35.0	41.0
36-37	38.723952290211024	41.0	38.0	41.0	35.0	41.0
38-39	38.5837168134245	41.0	38.0	41.0	35.0	41.0
40-41	38.4618673740934	41.0	38.0	41.0	35.0	41.0
42-43	38.296050449822104	41.0	38.0	41.0	35.0	41.0
44-45	38.04311840679529	41.0	38.0	41.0	34.0	41.0
46-47	37.65897730339225	40.0	38.0	41.0	33.0	41.0
48-49	37.31909055809493	40.0	38.0	41.0	33.0	41.0
50-51	37.125991373047206	40.0	37.5	41.0	32.5	41.0
52-53	36.974333030269065	40.0	37.0	41.0	32.0	41.0
54-55	36.81214291348553	40.0	37.0	41.0	32.0	41.0
56-57	36.62582373283847	40.0	36.0	41.0	31.0	41.0
58-59	36.424348598639114	40.0	35.5	41.0	31.0	41.0
60-61	36.21227152898	39.0	35.0	41.0	31.0	41.0
62-63	35.970199731808876	39.0	35.0	41.0	30.5	41.0
64-65	35.69496772157942	39.0	35.0	41.0	30.0	41.0
66-67	35.406799018569956	38.0	35.0	41.0	29.5	41.0
68-69	35.13672765316163	37.0	35.0	40.0	29.0	41.0
70-71	34.883695806503844	37.0	35.0	40.0	29.0	41.0
72-73	34.6320888601047	36.5	35.0	39.0	29.0	41.0
74-75	34.33977531728338	36.0	35.0	39.0	29.0	41.0
76-77	34.03032975883557	35.5	35.0	39.0	29.0	40.5
78-79	33.723726943243946	35.0	35.0	37.0	29.0	39.5
80-81	33.43088523827909	35.0	35.0	37.0	29.0	39.0
82-83	33.133467511925375	35.0	34.0	36.5	29.0	39.0
84-85	32.887123504917525	35.0	34.0	36.0	29.0	38.0
86-87	32.676853113466436	35.0	34.0	36.0	29.0	37.0
88-89	32.47038057399543	35.0	34.0	35.5	28.0	37.0
90-91	32.282228726341266	35.0	34.0	35.0	27.0	36.5
92-93	32.142697853225556	35.0	34.0	35.0	27.0	36.0
94-95	32.01699185878036	35.0	34.0	35.0	27.0	36.0
96-97	31.89267971088499	35.0	34.0	35.0	27.0	36.0
98-99	31.777828731323147	35.0	34.0	35.0	26.5	36.0
100	31.67176777804071	35.0	34.0	35.0	26.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.26411193460112514
1101	2	0.24693894775686687
1101	3	0.25551179754322817
1101	4	0.17890974731346176
1101	5	0.20767498652207195
1101	6	0.2289068012441149
1101	7	0.24367255356732187
1101	8	0.26757348158468375
1101	9	0.3275080601726188
1101	10-11	0.3378093406860003
1101	12-13	0.3130452916925677
1101	14-15	0.3740420488954115
1101	16-17	0.4028835491781706
1101	18-19	0.3902943817057789
1101	20-21	0.38255948986448374
1101	22-23	0.40122811764905464
1101	24-25	0.360812591654728
1101	26-27	0.35613854914338106
1101	28-29	0.3739258430870436
1101	30-31	0.41374319001158
1101	32-33	0.4493075099600077
1101	34-35	0.4314697092289137
1101	36-37	0.4058761755121765
1101	38-39	0.41262120002037506
1101	40-41	0.4090052409074616
1101	42-43	0.42964301084502665
1101	44-45	0.4630435630560328
1101	46-47	0.48744770998305853
1101	48-49	0.5169340942706953
1101	50-51	0.5295443077424196
1101	52-53	0.5106251520419249
1101	54-55	0.5027865650987806
1101	56-57	0.5011341268432616
1101	58-59	0.505584969800843
1101	60-61	0.5239946224469492
1101	62-63	0.46892125473214463
1101	64-65	0.4808303523271391
1101	66-67	0.47674859407778314
1101	68-69	0.43841465849423145
1101	70-71	0.418065732842841
1101	72-73	0.4009200094739782
1101	74-75	0.3821829796507501
1101	76-77	0.3854728721327376
1101	78-79	0.35321757358341443
1101	80-81	0.32338826525523245
1101	82-83	0.390001162817434
1101	84-85	0.36139517559782774
1101	86-87	0.3002049046283659
1101	88-89	0.3585472973249537
1101	90-91	0.37597732622754165
1101	92-93	0.41536785050261216
1101	94-95	0.38391626548730784
1101	96-97	0.38656790070742986
1101	98-99	0.3974385511494063
1101	100	0.42081565578136093
1102	1	0.1428922166849702
1102	2	0.18169477496174835
1102	3	0.21809086353452045
1102	4	0.1326490947189356
1102	5	0.11949553147923098
1102	6	0.14530515848219494
1102	7	0.17384177057338945
1102	8	0.21548723753035404
1102	9	0.24773396358992272
1102	10-11	0.24538223535448367
1102	12-13	0.27417730495880477
1102	14-15	0.34209156278644315
1102	16-17	0.3475452670466197
1102	18-19	0.34212970716498603
1102	20-21	0.3003051995061625
1102	22-23	0.3246127560240524
1102	24-25	0.3184703593992708
1102	26-27	0.32344648187572034
1102	28-29	0.30923463284673147
1102	30-31	0.29953772157321623
1102	32-33	0.3604061878730178
1102	34-35	0.3061592631164345
1102	36-37	0.3146192991732377
1102	38-39	0.331647133810975
1102	40-41	0.354819401287628
1102	42-43	0.3766042262478706
1102	44-45	0.41080891945100007
1102	46-47	0.37770918311404955
1102	48-49	0.3903877878279758
1102	50-51	0.3313122415742882
1102	52-53	0.28072284098706035
1102	54-55	0.3352849623737484
1102	56-57	0.2971131860475893
1102	58-59	0.26587478790269614
1102	60-61	0.26974011246548457
1102	62-63	0.30754336296209317
1102	64-65	0.31375906381529717
1102	66-67	0.3023569294991475
1102	68-69	0.2969228502101515
1102	70-71	0.2862782186304216
1102	72-73	0.2542458745094933
1102	74-75	0.22766458297144254
1102	76-77	0.22068032908411794
1102	78-79	0.22087468197837978
1102	80-81	0.18161369499716074
1102	82-83	0.253611970383119
1102	84-85	0.27503251178091404
1102	86-87	0.25893932993065505
1102	88-89	0.27721903372000867
1102	90-91	0.3053790060988746
1102	92-93	0.2714589802116123
1102	94-95	0.29158300696687434
1102	96-97	0.2903140470817043
1102	98-99	0.30181653065856295
1102	100	0.3427877552536458
1103	1	0.18090071169630306
1103	2	0.1944749955664662
1103	3	0.16602846863034415
1103	4	0.13761716750475017
1103	5	0.12123255644616648
1103	6	0.17894790069983912
1103	7	0.15929633826789313
1103	8	0.18145543721245616
1103	9	0.2436890273303618
1103	10-11	0.22485822745789363
1103	12-13	0.24515545865860844
1103	14-15	0.2867125032092446
1103	16-17	0.3135135157126214
1103	18-19	0.2254263774510008
1103	20-21	0.26169943684179486
1103	22-23	0.2849638392882383
1103	24-25	0.2624205913121287
1103	26-27	0.28868504541986084
1103	28-29	0.3330061611469688
1103	30-31	0.331681157675753
1103	32-33	0.32407218638533664
1103	34-35	0.3260564730678368
1103	36-37	0.35718163114710677
1103	38-39	0.34438786639706365
1103	40-41	0.34887674488837206
1103	42-43	0.40008975156930404
1103	44-45	0.4397134213272409
1103	46-47	0.4477761831483278
1103	48-49	0.4366363609795343
1103	50-51	0.4346403642805683
1103	52-53	0.4676827943980584
1103	54-55	0.4270104443553464
1103	56-57	0.41200406259105193
1103	58-59	0.39737356373212407
1103	60-61	0.346764407344736
1103	62-63	0.384605893271754
1103	64-65	0.42663626833732593
1103	66-67	0.4451788369396539
1103	68-69	0.38122889527228665
1103	70-71	0.4035221626217904
1103	72-73	0.40429921867301744
1103	74-75	0.3854281044758494
1103	76-77	0.3782041094015298
1103	78-79	0.3520863561901777
1103	80-81	0.34195183111761196
1103	82-83	0.3843483048590528
1103	84-85	0.3988843904666126
1103	86-87	0.37005393289384614
1103	88-89	0.3868051991448169
1103	90-91	0.46529169608135135
1103	92-93	0.47287893801184566
1103	94-95	0.4740705976313393
1103	96-97	0.4602769451386699
1103	98-99	0.47542287896040847
1103	100	0.47988122687159773
1104	1	0.07330306901353367
1104	2	0.12912568117985757
1104	3	0.1431679953701348
1104	4	0.03141949788810905
1104	5	0.08783610435184386
1104	6	0.09069935461690193
1104	7	0.07826028910314164
1104	8	0.12010314053152626
1104	9	0.13615257223441546
1104	10-11	0.1648907756373461
1104	12-13	0.1834858812630742
1104	14-15	0.19036942238564336
1104	16-17	0.2673641481222262
1104	18-19	0.21975998720809287
1104	20-21	0.20251655995179618
1104	22-23	0.2328064957461038
1104	24-25	0.26807749437516293
1104	26-27	0.28290746758811025
1104	28-29	0.288951388783083
1104	30-31	0.284911034324729
1104	32-33	0.30426006682479567
1104	34-35	0.31504022430874556
1104	36-37	0.2891466283926505
1104	38-39	0.2895738358377855
1104	40-41	0.2820869783516784
1104	42-43	0.2744098072097856
1104	44-45	0.29221106042971456
1104	46-47	0.24611838820941045
1104	48-49	0.2742661114908671
1104	50-51	0.19674959891387545
1104	52-53	0.2220668802705532
1104	54-55	0.24223912108525525
1104	56-57	0.23483106621427652
1104	58-59	0.17632132806199508
1104	60-61	0.2034415607864588
1104	62-63	0.2057075526955714
1104	64-65	0.2316118070003057
1104	66-67	0.25825850548539364
1104	68-69	0.2911484395335435
1104	70-71	0.27125972136959575
1104	72-73	0.23335825358768858
1104	74-75	0.23333230209680522
1104	76-77	0.23153440035957118
1104	78-79	0.25394003642716
1104	80-81	0.19768625172764587
1104	82-83	0.2403705999746606
1104	84-85	0.22733370875740633
1104	86-87	0.20650487768943293
1104	88-89	0.22012030710629205
1104	90-91	0.19006617211809385
1104	92-93	0.2529865471929966
1104	94-95	0.2382687864652766
1104	96-97	0.20973274471178271
1104	98-99	0.2147937658445187
1104	100	0.18399561435261447
1105	1	0.003704520148062329
1105	2	0.043689270740031816
1105	3	0.07270870259182516
1105	4	-0.0562961962806412
1105	5	-0.10705090621117108
1105	6	-0.09185862121088917
1105	7	-0.45560354859505026
1105	8	-0.39270939054810583
1105	9	-0.344269026163218
1105	10-11	-0.313201981130689
1105	12-13	-0.234388927329789
1105	14-15	-0.20247627322896022
1105	16-17	-0.14134234035966387
1105	18-19	-0.15874427303558036
1105	20-21	-0.13587320709908113
1105	22-23	-0.093160728154146
1105	24-25	-0.0981883239792154
1105	26-27	-0.07003520176571953
1105	28-29	-0.11901068700800721
1105	30-31	-0.14384108593841205
1105	32-33	-0.14755946956820765
1105	34-35	-0.12013907901008736
1105	36-37	-0.0921289077582017
1105	38-39	-0.0950706544017379
1105	40-41	-0.12674645266905316
1105	42-43	-0.14908025258325353
1105	44-45	-0.17751441198950602
1105	46-47	-0.15878427882606871
1105	48-49	-0.10106950784782498
1105	50-51	-0.08402353172943577
1105	52-53	-0.10957471308626054
1105	54-55	-0.11470040753577848
1105	56-57	-0.09234696656719876
1105	58-59	-0.07905125961276838
1105	60-61	-0.07469714264433946
1105	62-63	-0.07748261216374175
1105	64-65	-0.05870110086582514
1105	66-67	-0.03497470457043761
1105	68-69	-0.025135893365046513
1105	70-71	-0.06571605550964676
1105	72-73	-0.024018333814453285
1105	74-75	-0.04355351332682034
1105	76-77	-0.06483784740048293
1105	78-79	-0.07184041473646374
1105	80-81	-0.09535291022865522
1105	82-83	-0.08159961548971495
1105	84-85	-0.04751221153232876
1105	86-87	-0.06045429156366566
1105	88-89	-0.03203097634230545
1105	90-91	-0.006390865826588765
1105	92-93	0.0019782686843470287
1105	94-95	-0.008893638933777481
1105	96-97	-0.020823790309375312
1105	98-99	-0.029597363306173463
1105	100	-0.00873814644750226
1106	1	0.05898497679524439
1106	2	0.0356026024947127
1106	3	0.07270900170569661
1106	4	0.040039683674990556
1106	5	0.06718940686135255
1106	6	0.06468063125368673
1106	7	0.1155023003275133
1106	8	0.10036502649140999
1106	9	0.0853484321166249
1106	10-11	0.10980805164720664
1106	12-13	0.06845301591335584
1106	14-15	0.1037018615467673
1106	16-17	0.14646941730824636
1106	18-19	0.13994238025404826
1106	20-21	0.11899014771228877
1106	22-23	0.12276850648822801
1106	24-25	0.12437960602813547
1106	26-27	0.12027162675478564
1106	28-29	0.1208555717005737
1106	30-31	0.18710544745639623
1106	32-33	0.13193657340448794
1106	34-35	0.14865213950506018
1106	36-37	0.12105251727015087
1106	38-39	0.0855645886906018
1106	40-41	0.1532349025660622
1106	42-43	0.1991362809361945
1106	44-45	0.262057867632123
1106	46-47	0.24549707516273855
1106	48-49	0.26094906213859304
1106	50-51	0.2896789372063395
1106	52-53	0.24341739778527938
1106	54-55	0.25099792365806195
1106	56-57	0.24372813594163034
1106	58-59	0.2575559789430102
1106	60-61	0.2065099438618887
1106	62-63	0.19923082428078942
1106	64-65	0.2378146836226236
1106	66-67	0.2942153199818307
1106	68-69	0.29426238922279424
1106	70-71	0.26244362144736755
1106	72-73	0.2831219630560753
1106	74-75	0.33020106373862745
1106	76-77	0.26444669713633573
1106	78-79	0.2430512312622639
1106	80-81	0.2505591004397374
1106	82-83	0.2876879326489288
1106	84-85	0.2812763149579425
1106	86-87	0.2902141531688116
1106	88-89	0.3127685176567212
1106	90-91	0.2912280755595589
1106	92-93	0.30684942538262305
1106	94-95	0.2972822774140411
1106	96-97	0.2864186913387421
1106	98-99	0.23787263183322693
1106	100	0.22494068002906076
1107	1	-0.01981869906106226
1107	2	-0.004727389151071293
1107	3	0.017513512983896362
1107	4	-0.050906013902285
1107	5	-0.07639455303839782
1107	6	-0.0844964781865798
1107	7	-0.06592627110105553
1107	8	-0.04835844886363816
1107	9	-0.015943130122302307
1107	10-11	-0.012298046932571083
1107	12-13	0.0391514286117669
1107	14-15	0.010853072908503236
1107	16-17	0.03327016918795067
1107	18-19	0.009490417012614216
1107	20-21	0.02769858547335957
1107	22-23	0.010918882428079257
1107	24-25	0.02704418414008103
1107	26-27	-0.003167387447390979
1107	28-29	0.009477961007171132
1107	30-31	0.05753819599441812
1107	32-33	0.048837993621702935
1107	34-35	-0.0044021629176711485
1107	36-37	-0.006675552905285542
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2216	54-55	-0.8645478359685228
2216	56-57	-0.873026136465306
2216	58-59	-0.9647903745748252
2216	60-61	-0.9574240314977231
2216	62-63	-1.0270515181320974
2216	64-65	-0.9575326715782424
2216	66-67	-0.8745537285209863
2216	68-69	-0.944832079147929
2216	70-71	-0.9580696572086538
2216	72-73	-1.0038973028170446
2216	74-75	-0.9303875496168104
2216	76-77	-0.8774188129338825
2216	78-79	-0.920378221269651
2216	80-81	-0.9188855197088373
2216	82-83	-0.90573820466809
2216	84-85	-0.950136286926444
2216	86-87	-0.9105821492479365
2216	88-89	-0.8582330077489075
2216	90-91	-0.8757915716649656
2216	92-93	-1.0210082390179664
2216	94-95	-0.9742880717513032
2216	96-97	-1.092911325084792
2216	98-99	-1.0823589755883845
2216	100	-1.0981049245146153
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	82.0
3	587.0
4	321.0
5	481.0
6	603.0
7	1080.0
8	2090.0
9	3135.0
10	4394.0
11	6864.0
12	11640.0
13	17770.0
14	18813.0
15	24901.0
16	33614.0
17	54852.0
18	66955.0
19	32087.0
20	17670.0
21	15236.0
22	14655.0
23	14779.0
24	16358.0
25	19612.0
26	24315.0
27	30630.0
28	37352.0
29	46459.0
30	58936.0
31	74203.0
32	93594.0
33	119635.0
34	168009.0
35	273652.0
36	500636.0
37	1161902.0
38	1948154.0
39	1064405.0
40	41364.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.27382190449017	21.60237862808709	14.824042571636689	23.29975689578605
2	13.570138620766961	22.191162978000854	40.78353323120483	23.45516517002736
3	15.283585143130626	29.26257849180487	31.82026591021599	23.63357045484852
4	10.39432582655642	18.34873050167146	40.50779933141471	30.74914434035741
5	12.080806067927913	38.355315871849484	34.94588434569255	14.617993714530064
6	27.729052239146768	40.10551618487751	18.58380142232629	13.581630153649431
7	24.058769559062245	34.18383961672748	24.56247067956973	17.194920144640538
8	24.659119120864524	34.238607066794536	21.93072698060804	19.171546831732904
9	25.9742021729293	15.045189788809871	22.72536980068335	36.25523823757748
10-11	23.44769235240147	26.92073416281609	29.040988404677986	20.590585080104454
12-13	24.626271935833405	25.744388121541228	29.63536801551025	19.99397192711512
14-15	22.303404698741662	25.67365375114687	28.4969506752521	23.525990874859364
16-17	20.224616291572737	29.488659999252718	28.43889518542967	21.847828523744877
18-19	19.76559431733735	27.046426955283493	32.703989239142615	20.48398948823654
20-21	22.356411885101277	26.136893715775532	31.608523993971925	19.898170405151262
22-23	21.11320119448888	26.57620678789519	32.39798250446605	19.91260951314988
24-25	20.88717191088642	26.151427130715703	31.980901788753087	20.980499169644794
26-27	20.60100191064308	26.772542636584497	32.11320977492672	20.5132456778457
28-29	20.32093891346546	26.375496967751506	32.54061223537469	20.76295188340835
30-31	21.532822866216648	26.9184676620435	31.377278940744613	20.171430530995238
32-33	20.01701875093362	26.8589784415039	32.78583152449597	20.338171283066515
34-35	20.787267436868692	26.039315211382075	32.217787584797705	20.955629766951528
36-37	21.2176387177805	27.033728795418433	31.34225581533031	20.406376671470756
38-39	20.702635557987506	26.694438516793888	31.775026425327795	20.827899499890812
40-41	20.970028451610464	26.6737688440542	31.342465139200325	21.013737565135006
42-43	20.4265703764649	26.83461654673983	31.92442653948867	20.8143865373066
44-45	21.13209680926773	26.314916330243143	31.118787664717168	21.43419919577196
46-47	21.447185866488176	27.288493160034783	30.324323233661843	20.939997739815198
48-49	21.22178827300871	26.9485782913723	30.079165378270385	21.750468057348606
50-51	22.035913874124166	27.161319330246474	28.91015802254072	21.892608773088632
52-53	21.426343816482152	27.48117970255851	29.520645160111812	21.571831320847522
54-55	21.96073898821964	27.249472375457113	29.03013566871439	21.75965296760886
56-57	21.846888792885633	27.376983557952446	29.09165743421823	21.684470214943687
58-59	21.441568876611615	26.59711532449714	29.763376698924816	22.19793909996643
60-61	22.314037578082864	27.238480536078203	28.74175828920484	21.7057235966341
62-63	21.356905921377656	27.19766017777003	29.843220286208915	21.6022136146434
64-65	21.66660439318645	26.613393780124795	29.536419607012824	22.18358221967593
66-67	21.736075222890758	27.168634743458085	29.18231540686282	21.91297462678834
68-69	21.847258808119683	27.099328895282337	29.137976057773546	21.915436238824434
70-71	22.36886943040081	27.01436203814599	28.58238891689866	22.034379614554542
72-73	21.67154254502085	27.22826699764075	29.370775052928344	21.72941540441006
74-75	22.009496952781436	26.695482902645274	28.96597999143434	22.329040153138948
76-77	22.227251161734845	27.37682986834696	28.573620602300338	21.822298367617858
78-79	21.949232210922666	27.20059431021206	29.034315652322217	21.815857826543056
80-81	22.201474397323384	27.19196516978154	28.632867427543257	21.973693005351823
82-83	21.540522654940293	27.49191058088547	29.41599251381034	21.55157425036389
84-85	21.759016621032227	26.78249975754629	28.895824282835207	22.562659338586275
86-87	21.93182802293229	27.555598067658842	28.677602074922763	21.8349718344861
88-89	21.219772268745267	27.14565225008623	29.0480403776512	22.586535103517303
90-91	21.599308504079602	27.14952644403122	28.49512868192724	22.756036369961944
92-93	21.087076956494734	27.541706924101682	29.225469646087742	22.14574647331584
94-95	21.193582915052282	26.649031758693482	29.069632949190922	23.087752377063318
96-97	21.124649652361317	27.56774148817672	28.806279292508467	22.501329566953505
98-99	20.623096048815547	27.103455044499153	29.183145988300957	23.09030291838435
100	21.406553229169052	27.050694810485247	28.41964035723147	23.123111603114232
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1104.0
1	791.0
2	1370.0
3	2112.5
4	1305.5
5	1000.5
6	2471.5
7	6930.0
8	12410.0
9	12694.0
10	7923.5
11	4474.0
12	4130.5
13	4487.5
14	4966.0
15	5434.5
16	6007.0
17	6876.0
18	7864.5
19	8986.0
20	10827.5
21	13357.0
22	16719.0
23	22077.0
24	28745.5
25	35703.0
26	45409.5
27	58960.0
28	76368.5
29	93350.5
30	106103.5
31	119136.5
32	132958.0
33	144691.0
34	156945.0
35	173429.5
36	187834.0
37	198253.0
38	211050.0
39	221179.0
40	227578.5
41	226297.0
42	218360.0
43	213155.0
44	205341.0
45	194274.5
46	188108.0
47	183210.0
48	175232.5
49	175317.0
50	179195.0
51	173760.0
52	163141.0
53	154886.5
54	151370.0
55	147982.0
56	134759.5
57	119142.5
58	111027.0
59	105598.0
60	93885.0
61	75947.5
62	60175.0
63	47298.0
64	37034.5
65	29644.0
66	22272.5
67	15416.0
68	10911.0
69	7248.0
70	5099.5
71	4380.5
72	3162.5
73	1822.5
74	1258.5
75	961.0
76	598.5
77	409.0
78	294.0
79	135.5
80	65.0
81	34.0
82	19.0
83	15.0
84	14.5
85	11.5
86	7.5
87	5.5
88	4.0
89	5.5
90	6.0
91	4.5
92	5.0
93	4.0
94	3.5
95	9.0
96	14.0
97	15.0
98	8.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008950774889672152
2	0.0
3	2.9891270503543365E-4
4	0.0025739705155829002
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	4.151565347714356E-5
24-25	7.472817625885841E-5
26-27	5.064909724211514E-4
28-29	9.299506378880158E-4
30-31	0.0014198353489183098
32-33	0.0038443495119834933
34-35	0.0047826032805669385
36-37	0.0036450743752932044
38-39	0.0023248765947200393
40-41	0.0032797366246943406
42-43	4.732784496394365E-4
44-45	4.234596654668643E-4
46-47	0.0032133115791309116
48-49	0.002108995196638893
50-51	9.548600299743019E-4
52-53	3.321252278171484E-5
54-55	3.9855027338057817E-4
56-57	6.393410635480108E-4
58-59	6.393410635480108E-4
60-61	2.8230644364457617E-4
62-63	0.0
64-65	0.0
66-67	6.808567170251543E-4
68-69	6.227348021571534E-4
70-71	0.0019429325827303183
72-73	6.642504556342969E-5
74-75	0.001727051184649172
76-77	1.9927513669028908E-4
78-79	8.967381151063008E-4
80-81	7.057661091114404E-4
82-83	0.0011624382973600197
84-85	9.46556899278873E-4
86-87	1.7436574460400294E-4
88-89	0.001295288388486879
90-91	0.0016191104856085987
92-93	0.00270682060670976
94-95	9.382537685834444E-4
96-97	0.002117298327334321
98-99	0.002275057810547467
100	0.0030389458345269084
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6021825.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.18065238366577
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.15919343615543	30.342961730063074
2	16.85086157973699	15.226658387987321
3	6.468992987159177	8.768199702756313
4	3.235320927205968	5.846956406467683
5	1.7856218208258179	4.033777938770981
6	1.1301011505241496	3.063522434412741
7	0.7496764904638407	2.3709611041097314
8	0.5114899187672571	1.8487558574058311
9	0.35718352292141625	1.452400612765706
>10	1.5828968253186328	12.163054239482417
>50	0.09113832197558755	2.8581086667831475
>100	0.06694591509385707	6.080799015627458
>500	0.0072234112385665674	2.161521265023107
>1k	0.0032431313185227245	2.438821842900393
>5k	0.0	0.0
>10k+	1.1056129492256783E-4	1.3435007954440648
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37021	0.6147804029509326	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24545	0.40760068583859543	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19121	0.31752832405458475	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	1.660626139085742E-5	0.0
6	0.0	0.0	0.0	1.660626139085742E-5	0.0
7	0.0	0.0	0.0	1.660626139085742E-5	0.0
8	0.0	0.0	0.0	1.660626139085742E-5	0.0
9	0.0	0.0	0.0	1.660626139085742E-5	0.0
10-11	0.0	0.0	0.0	1.660626139085742E-5	8.30313069542871E-6
12-13	0.0	0.0	0.0	4.151565347714356E-5	1.660626139085742E-5
14-15	0.0	0.0	0.0	4.981878417257227E-5	2.4909392086286132E-5
16-17	0.0	0.0	0.0	4.981878417257227E-5	3.321252278171484E-5
18-19	0.0	0.0	0.0	1.0794069904057324E-4	3.321252278171484E-5
20-21	0.0	0.0	0.0	2.6570018225371874E-4	3.321252278171484E-5
22-23	0.0	1.660626139085742E-5	0.0	5.895222793754385E-4	4.981878417257227E-5
24-25	0.0	1.660626139085742E-5	0.0	0.0010295882062331602	4.981878417257227E-5
26-27	0.0	1.660626139085742E-5	0.0	0.001469654133090882	4.981878417257227E-5
28-29	0.0	1.660626139085742E-5	0.0	0.0031551896642629104	5.812191486800098E-5
30-31	0.0	1.660626139085742E-5	0.0	0.0071656017901549785	6.642504556342969E-5
32-33	0.0	1.660626139085742E-5	0.0	0.01533588239445683	6.642504556342969E-5
34-35	0.0	1.660626139085742E-5	0.0	0.026835718407625594	6.642504556342969E-5
36-37	0.0	1.660626139085742E-5	0.0	0.04418095843037617	6.642504556342969E-5
38-39	0.0	1.660626139085742E-5	0.0	0.06970478218812404	6.642504556342969E-5
40-41	0.0	1.660626139085742E-5	0.0	0.09841700813291651	6.642504556342969E-5
42-43	0.0	1.660626139085742E-5	0.0	0.12479605435229352	6.642504556342969E-5
44-45	0.0	1.660626139085742E-5	0.0	0.1559577038522375	1.0794069904057326E-4
46-47	0.0	1.660626139085742E-5	0.0	0.19305609179941297	1.3285009112685937E-4
48-49	0.0	1.660626139085742E-5	0.0	0.23466307971420625	1.4115322182228809E-4
50-51	0.0	1.660626139085742E-5	0.0	0.2815010399671196	1.6606261390857423E-4
52-53	0.0	1.660626139085742E-5	0.0	0.3270852274850232	1.8266887529943166E-4
54-55	0.0	1.660626139085742E-5	0.0	0.37706177114080863	1.8266887529943166E-4
56-57	0.0	1.660626139085742E-5	0.0	0.4364042462210377	1.8266887529943166E-4
58-59	0.0	1.660626139085742E-5	0.0	0.5036596048540102	1.8266887529943166E-4
60-61	0.0	1.660626139085742E-5	0.0	0.5794505818418835	2.1588139808114649E-4
62-63	0.0	1.660626139085742E-5	0.0	0.6569852162757968	2.1588139808114649E-4
64-65	0.0	1.660626139085742E-5	0.0	0.7395266385190535	2.1588139808114649E-4
66-67	0.0	1.660626139085742E-5	0.0	0.8323307303018603	2.1588139808114649E-4
68-69	0.0	1.660626139085742E-5	0.0	0.9420399961805599	2.241845287765752E-4
70-71	0.0	1.660626139085742E-5	0.0	1.0683804328422033	2.490939208628613E-4
72-73	0.0	1.660626139085742E-5	0.0	1.2007655486501185	2.490939208628613E-4
74-75	0.0	1.660626139085742E-5	0.0	1.3400754754580215	2.490939208628613E-4
76-77	0.0	1.660626139085742E-5	0.0	1.4872982858186679	2.5739705155829E-4
78-79	0.0	1.660626139085742E-5	0.0	1.6567236676588908	2.6570018225371874E-4
80-81	0.0	1.660626139085742E-5	0.0	1.8517725108252066	2.6570018225371874E-4
82-83	0.0	1.660626139085742E-5	0.0	2.062348208391974	3.0721583573086234E-4
84-85	0.0	1.660626139085742E-5	0.0	2.281359886745297	3.1551896642629103E-4
86-87	0.0	1.660626139085742E-5	0.0	2.513747908648956	3.1551896642629103E-4
88	0.0	1.660626139085742E-5	0.0	2.702586010055091	3.1551896642629103E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12475	0.0	65.712234	1
GTATCAA	28735	0.0	51.969234	1
TATCAAC	39745	0.0	37.34481	2
ATCAACG	40035	0.0	36.99212	3
TCAACGC	40410	0.0	36.648533	4
CAACGCA	42095	0.0	35.191833	5
AACGCAG	43315	0.0	34.244034	6
CGCAGAG	47505	0.0	30.946651	8
ACGCAGA	47680	0.0	30.931639	7
GTGGTAT	3775	0.0	30.257261	1
GCAGAGT	50795	0.0	28.636894	9
TGGTATC	3945	0.0	28.474203	2
GTACATG	31935	0.0	25.919865	1
TACATGG	31535	0.0	25.590464	2
ACATGGG	32520	0.0	24.179428	3
AGAGTAC	48785	0.0	23.978655	10-11
AGTACTT	33775	0.0	23.398483	12-13
GAGTACT	32705	0.0	22.798807	12-13
CAGAGTA	50235	0.0	22.117056	10-11
CATGGGG	21600	0.0	20.845331	4
>>END_MODULE