FastQCFastQC Report
Wed 25 May 2016
SRR2431363_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431363_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3062293
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA299450.9778620138569366No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49150.044.0830961
GTATCAA89250.039.8114741
ATCAACG110800.030.2867833
TCAACGC111750.030.029314
CAACGCA127550.026.3831885
TATCAAC135050.026.1708582
AACGCAG132900.025.3918386
GTACATG202650.025.326241
TACATGG195650.025.031252
ACGCAGA133500.024.5031917
CGCAGAG131950.024.18558
ACATGGG208350.022.4226843
GCAGAGT140650.021.3194319
CGTCACT5150.020.9901391
CATGGGG110150.020.609024
GTACAAA24650.020.4014741
ATGCGGG4600.019.41298
CGCAGAA6000.018.7998642
CATGGGA61550.018.555514
GTCGGGA14550.018.4122372
Produced by FastQC (version 0.11.5)